##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921105_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2657585
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.780735893677907	31.0	30.0	34.0	24.0	34.0
2	29.821738533292443	31.0	30.0	34.0	24.0	34.0
3	29.974232244688316	31.0	31.0	34.0	25.0	34.0
4	33.25919584886278	37.0	35.0	37.0	26.0	37.0
5	33.46235548439655	37.0	35.0	37.0	27.0	37.0
6	33.370276021274954	37.0	35.0	37.0	27.0	37.0
7	33.29135248731461	37.0	35.0	37.0	26.0	37.0
8	33.195780379555124	37.0	35.0	37.0	26.0	37.0
9	34.58189183036479	39.0	35.0	39.0	25.0	39.0
10-11	34.6434341704969	39.0	35.0	39.0	25.5	39.0
12-13	34.58091594436303	39.0	35.0	39.0	25.5	39.0
14-15	35.79108438676467	40.0	36.0	41.0	25.5	41.0
16-17	35.80732563586865	40.0	36.0	41.0	26.0	41.0
18-19	35.87587490146129	40.0	36.0	41.0	26.0	41.0
20-21	35.96037248103071	40.0	36.0	41.0	27.0	41.0
22-23	35.9214734429943	40.0	36.0	41.0	26.5	41.0
24-25	35.928125911306694	40.0	36.0	41.0	27.0	41.0
26-27	35.864618253038	40.0	36.0	41.0	27.0	41.0
28-29	35.71311905357684	40.0	36.0	41.0	27.0	41.0
30-31	35.57138774488868	40.0	35.5	41.0	26.0	41.0
32-33	35.41313598624315	40.0	35.0	41.0	25.0	41.0
34-35	35.25598334578198	40.0	35.0	41.0	24.5	41.0
36-37	35.08230517556353	40.0	35.0	41.0	24.0	41.0
38-39	34.907855063902005	40.0	35.0	41.0	23.0	41.0
40-41	34.76257579719934	40.0	35.0	41.0	22.0	41.0
42-43	34.544998937004834	40.0	34.5	41.0	19.5	41.0
44-45	34.34104666454695	39.0	34.0	41.0	18.0	41.0
46-47	34.12899192311817	39.0	34.0	41.0	15.0	41.0
48-49	33.87117081861916	39.0	33.0	41.0	10.5	41.0
50-51	33.69056060295343	39.0	33.0	41.0	8.0	41.0
52-53	33.51102184878376	38.5	33.0	41.0	6.0	41.0
54-55	33.29149528613384	38.0	33.0	40.0	2.0	41.0
56-57	33.055763032979186	38.0	33.0	40.0	2.0	41.0
58-59	32.792785179025316	38.0	32.0	40.0	2.0	41.0
60-61	32.50061032855017	37.0	32.0	40.0	2.0	41.0
62-63	32.21298697877961	37.0	31.5	40.0	2.0	41.0
64-65	31.89262714080641	36.0	31.0	40.0	2.0	41.0
66-67	31.55287620151378	36.0	31.0	39.0	2.0	41.0
68-69	31.18420577328665	35.0	31.0	39.0	2.0	40.5
70-71	30.807928815070824	35.0	30.5	38.5	2.0	40.0
72-73	30.441976456068197	35.0	30.0	37.5	2.0	40.0
74-75	30.38683936732033	35.0	30.0	37.0	2.0	39.0
76-77	30.395735225778292	35.0	31.0	37.0	2.0	39.0
78-79	30.189004490919388	35.0	31.0	36.0	2.0	39.0
80-81	29.94147111004916	35.0	31.0	36.0	2.0	38.0
82-83	29.665617656631866	35.0	31.0	35.5	2.0	37.0
84-85	29.389013145393278	35.0	30.5	35.0	2.0	37.0
86-87	29.209180327252	35.0	30.0	35.0	2.0	36.0
88-89	29.000253801853937	35.0	30.0	35.0	2.0	36.0
90-91	28.82419376990764	35.0	30.0	35.0	2.0	36.0
92-93	28.669824483506645	35.0	30.0	35.0	2.0	35.5
94-95	28.52617319859948	35.0	29.0	35.0	2.0	35.0
96-97	28.400475431641887	35.0	29.0	35.0	2.0	35.0
98-99	28.264527569202865	35.0	29.0	35.0	2.0	35.0
100-101	28.095090655614026	34.0	29.0	35.0	2.0	35.0
102-103	26.8376544870625	32.5	26.0	34.5	2.0	35.0
104-105	27.701350662349462	34.0	27.5	35.0	2.0	35.0
106-107	27.799179706387566	34.0	29.0	35.0	2.0	35.0
108-109	27.70385105274149	34.0	28.5	35.0	2.0	35.0
110-111	27.56039938515607	34.0	27.0	35.0	2.0	35.0
112-113	27.399263805296915	34.0	27.0	35.0	2.0	35.0
114-115	27.20807537670479	34.0	27.0	35.0	2.0	35.0
116-117	27.016486584624765	34.0	25.5	35.0	2.0	35.0
118-119	26.747903641840242	34.0	25.0	35.0	2.0	35.0
120-121	26.463511421083425	34.0	25.0	35.0	2.0	35.0
122-123	26.08629357104288	34.0	23.5	35.0	2.0	35.0
124-125	24.78065894411656	32.5	10.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94768.0
3	14469.0
4	10584.0
5	12845.0
6	22998.0
7	22901.0
8	16246.0
9	16400.0
10	17345.0
11	18155.0
12	18658.0
13	19117.0
14	20314.0
15	17766.0
16	12088.0
17	10511.0
18	9885.0
19	9852.0
20	10301.0
21	10945.0
22	13045.0
23	17075.0
24	22677.0
25	28819.0
26	31428.0
27	33397.0
28	38616.0
29	44926.0
30	53211.0
31	65389.0
32	82626.0
33	107147.0
34	148474.0
35	222597.0
36	408265.0
37	686276.0
38	266977.0
39	492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.87214403372158	18.479132507023756	11.68481491055441	23.963908548700253
2	16.054437188930617	22.068162837125975	37.79836114585742	24.079038828085995
3	19.008951945990077	26.73668957248169	29.05933017813514	25.19502830339309
4	12.239637958882861	17.836256759367462	36.13127869400679	33.79282658774289
5	12.808370085531246	40.49533551524008	32.94327670168012	13.753017697548556
6	30.951430998788744	37.75346470680207	17.335345189722958	13.959759104686231
7	27.093630018503895	35.05313089548011	20.50033602353069	17.352903062485307
8	25.634660320973822	36.455575943575866	19.561750901921904	18.3480128335284
9	26.465996535571424	17.583444280942704	18.72336032995706	37.22719885352881
10-11	24.10081006473512	28.417539462736535	26.895358257880588	20.58629221464776
12-13	24.858013417475483	26.49165235078863	26.63173234646515	22.018601885270737
14-15	22.63879224219186	27.400934650720664	24.705984269318012	25.254288837769458
16-17	22.216505436355757	29.792826567887637	25.56464821647267	22.426019779283937
18-19	22.270455775060903	28.59785174886489	27.5273983089005	21.604294167173702
20-21	23.825854071537577	27.652397366038407	26.985338758735804	21.53640980368821
22-23	23.203518236031943	27.633810040408203	26.542438402062924	22.62023332149693
24-25	22.52131161802591	27.854079039265045	27.061607528036845	22.5630018146722
26-27	22.46206078447731	27.989492292571367	26.95228085386705	22.596166069084276
28-29	22.53413312483617	28.311737908219065	26.942590215698146	22.211538751246614
30-31	22.68511465941682	28.27199202506816	26.763417071498523	22.279476244016493
32-33	22.025434786989166	28.54183015139557	26.75757971228194	22.67515534933332
34-35	22.35332864822602	28.489557558652344	26.906055312889094	22.251058480232537
36-37	22.597502327009906	28.249363567734115	26.647751806198443	22.505382299057537
38-39	22.29389057280328	28.329320935003665	26.744489495273804	22.632298996919246
40-41	22.402380540340626	28.390527175870854	26.92057891782357	22.286513365964954
42-43	22.28501707323273	28.47092184159316	26.76523669516442	22.478824390009688
44-45	22.306786520272652	28.46779207349977	26.682810075264356	22.542611330963222
46-47	22.175890391049315	28.502303318929233	26.623990927169412	22.69781536285204
48-49	22.019170613869694	28.41378071668869	26.61615677633361	22.950891893108004
50-51	21.98840093804646	28.536990931313856	26.446060822402263	23.028547308237428
52-53	22.096687577530748	28.382656761811226	26.306922728720895	23.213732931937134
54-55	22.04745731050886	28.348032830813075	26.300748762027826	23.303761096650238
56-57	22.161706118475006	28.33468140220506	26.218247799226564	23.28536468009337
58-59	22.032378790391828	27.941443085784236	26.47339724648685	23.55278087733709
60-61	22.17353063625088	28.06262240500449	26.424341868033352	23.339505090711278
62-63	22.089453307422303	27.956625389795274	26.52114309817568	23.43277820460674
64-65	22.021480311283753	27.925590906266535	26.560796548990663	23.49213223345905
66-67	22.169581299755272	28.15030597340059	26.40079182926191	23.279320897582227
68-69	22.580311700342882	27.994791813770625	26.317213563879367	23.10768292200713
70-71	22.149258823675616	27.95196730034672	26.538944002145033	23.359829873832634
72-73	22.389505895685847	27.901828618008317	26.518227350297018	23.19043813600882
74-75	22.353531364769566	27.894031469376234	26.391838307281475	23.36059885857273
76-77	22.256280596983448	27.69686585951018	26.708365316024135	23.33848822748224
78-79	22.68189999924687	27.648799885524067	26.574270027639912	23.09503008758915
80-81	22.668162570725436	27.43002967140012	26.610483643633625	23.291324114240822
82-83	22.57767769509532	27.53344647246207	26.47743733094099	23.411438501501618
84-85	22.63507908352117	27.324572661604414	26.45491475642554	23.585433498448875
86-87	22.597111638181993	27.21231056141083	26.461617431400654	23.728960369006526
88-89	22.724969967858407	27.11520689091042	26.431993319036884	23.727829822194284
90-91	22.787984650711028	27.211563412230245	26.424778654410137	23.575673282648594
92-93	22.830745828348313	27.247241725647598	26.32688504436866	23.595127401635434
94-95	22.52525714750581	27.365078181391223	26.413715738852893	23.695948932250076
96-97	22.63683446854454	27.457669181115236	26.194363071008976	23.711133279331246
98-99	22.474953489961532	27.696165418899128	26.010996722221396	23.817884368917948
100-101	22.434193702194843	28.064944020723274	25.97227908174929	23.528583195332594
102-103	22.29924821683766	28.328348492433125	25.949179661547912	23.423223629181305
104-105	22.097669218769003	28.5852155292032	25.864483403552864	23.452631848474933
106-107	22.092799258826155	28.855125557975292	25.690524141346522	23.36155104185203
108-109	22.120232574562745	29.033685647701326	25.558758033002988	23.28732374473294
110-111	22.237173772795938	29.377744334511004	25.377658466663878	23.00742342602918
112-113	22.33462271618439	29.56217092306105	25.209543541901553	22.893662818853006
114-115	22.280046234101377	29.77790984651529	25.151806862276537	22.7902370571068
116-117	22.380424874390357	29.76205203872409	25.101780387101584	22.755742699783966
118-119	22.536267521833565	29.735338597289296	24.926286290116074	22.802107590761064
120-121	22.66293140256794	29.92111910260242	24.793625340128077	22.62232415470156
122-123	22.94673682204569	30.02902929254469	24.61624126400198	22.407992621407637
124-125	23.04852185574156	30.280523452920878	24.38943676827339	22.281517923064172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3906.0
1	2941.5
2	1743.0
3	1419.5
4	1180.5
5	940.5
6	851.5
7	876.0
8	956.5
9	1017.0
10	984.5
11	976.5
12	1113.5
13	1255.5
14	1350.0
15	1634.5
16	1993.5
17	2340.0
18	2869.0
19	3503.5
20	4271.5
21	5321.0
22	6568.0
23	8058.0
24	9823.0
25	12131.5
26	15804.5
27	19873.0
28	22573.0
29	26053.5
30	30379.0
31	34549.5
32	39775.0
33	43923.5
34	47895.0
35	52389.0
36	57656.0
37	62418.5
38	65540.0
39	68842.0
40	73615.0
41	78499.5
42	81459.5
43	85624.5
44	90535.5
45	94857.0
46	98554.0
47	102049.0
48	105538.0
49	107885.5
50	111544.5
51	110659.0
52	101671.5
53	95026.0
54	91148.5
55	84841.5
56	76447.5
57	68043.0
58	62325.0
59	55796.0
60	47085.0
61	38634.0
62	30910.5
63	23387.0
64	17144.5
65	13216.5
66	9963.5
67	7438.5
68	5719.5
69	4416.0
70	3637.0
71	2993.5
72	2417.5
73	2179.0
74	1858.0
75	1464.0
76	985.5
77	635.0
78	442.0
79	307.0
80	212.0
81	126.5
82	83.0
83	64.0
84	53.5
85	43.5
86	41.0
87	34.5
88	28.5
89	27.5
90	19.0
91	15.0
92	14.0
93	10.5
94	12.0
95	11.0
96	11.0
97	14.5
98	14.0
99	15.0
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2878553272990328
2	0.06874662522553371
3	0.07800314947593398
4	0.08263141160113412
5	0.09087197587283191
6	0.12492544923304429
7	0.11322309540428623
8	0.11198136654142765
9	0.11961988045537583
10-11	0.098284720902624
12-13	0.08716560335793587
14-15	0.09717469055552315
16-17	0.09916898236556873
18-19	0.09213251880937016
20-21	0.09109774475698801
22-23	0.07284809328770293
24-25	0.08596150264243665
26-27	0.0971370624081638
28-29	0.0900441566309262
30-31	0.08520893969524963
32-33	0.09484174541924341
34-35	0.08891531221014569
36-37	0.08855784481023184
38-39	0.07773975244441853
40-41	0.08991245811516847
42-43	0.07948946129662833
44-45	0.07570783248701358
46-47	0.06560467492102792
48-49	0.06778710746787027
50-51	0.06972495705687683
52-53	0.08229275827489996
54-55	0.08788053815776353
56-57	0.09604584613474264
58-59	0.06552941862630922
60-61	0.08313939159048535
62-63	0.08823800555767737
64-65	0.08014795387541697
66-67	0.07382642511904605
68-69	0.08090051682260398
70-71	0.08067474793844788
72-73	0.06086352835374974
74-75	0.09032636773612134
76-77	0.09418325284045477
78-79	0.07544443545549813
80-81	0.08784291001040419
82-83	0.09207607658833114
84-85	0.0969112935240077
86-87	0.0842494219375862
88-89	0.06323410163738882
90-91	0.08835088999975542
92-93	0.06443820235288805
94-95	0.09201963436729212
96-97	0.08022321017013567
98-99	0.07433440510839728
100-101	0.09053332254659777
102-103	0.0639490364372165
104-105	0.07021412297254838
106-107	0.08795579445248224
108-109	0.09491700171396211
110-111	0.08812512111559931
112-113	0.07717533023402826
114-115	0.09038280995716036
116-117	0.08411772342182847
118-119	0.07679904876043477
120-121	0.10861364735276575
122-123	0.08789935223144321
124-125	0.10930976807891374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2657585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.94190016863169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.87606231281765	28.717851915082083
2	13.591398288959782	11.672809369532452
3	6.400757133362313	8.245820214735048
4	3.6195945983795017	6.217290795781243
5	2.3924141794125884	5.136740542717715
6	1.5964870540259468	4.113371261669707
7	1.1772980172042586	3.5388789746459324
8	0.818098409823966	2.8104560194221664
9	0.615104855236936	2.3772394158142642
>10	2.77667333921793	19.573879131648777
>50	0.0894906701469643	2.559906871574546
>100	0.04309692309928203	3.2881579071642344
>500	0.0027312691924892647	0.858267868355762
>1k	7.929491203938298E-4	0.8893297118561889
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4898	0.1843026657660997	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3689	0.1388102356086447	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3283	0.1235332077807483	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3156	0.11875443306611076	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2864	0.10776701403718038	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.762814735935069E-5	0.0	0.0	0.0	0.0
7	3.762814735935069E-5	0.0	0.0	0.0	0.0
8	3.762814735935069E-5	0.0	0.0	0.0	0.0
9	3.762814735935069E-5	0.0	0.0	0.0	0.0
10-11	5.644222103902603E-5	0.0	0.0	0.0	0.0
12-13	7.525629471870137E-5	0.0	0.0	0.0	0.0
14-15	1.1288444207805206E-4	0.0	0.0	3.762814735935069E-5	0.0
16-17	1.693266631170781E-4	0.0	0.0	7.525629471870137E-5	0.0
18-19	1.8814073679675344E-4	0.0	0.0	1.316985157577274E-4	0.0
20-21	1.8814073679675344E-4	0.0	0.0	3.574673999138315E-4	0.0
22-23	2.633970315154548E-4	0.0	0.0	8.842614629447411E-4	0.0
24-25	3.010251788748055E-4	0.0	0.0	0.002389387357318769	0.0
26-27	3.1983925255448084E-4	0.0	0.0	0.004214352504247277	0.0
28-29	3.386533262341562E-4	0.0	0.0	0.01172116790243774	0.0
30-31	3.7628147359350687E-4	0.0	0.0	0.03450501112852458	0.0
32-33	3.7628147359350687E-4	0.0	0.0	0.08596150264243665	0.0
34-35	4.1390962095285756E-4	0.0	0.0	0.17228047268478713	0.0
36-37	4.5153776831220824E-4	0.0	0.0	0.31825887036538814	0.0
38-39	4.5153776831220824E-4	0.0	0.0	0.5578184705286943	0.0
40-41	4.5153776831220824E-4	0.0	0.0	0.8360409921037333	0.0
42-43	4.5153776831220824E-4	0.0	0.0	1.083859970612417	0.0
44-45	4.5153776831220824E-4	0.0	0.0	1.3693823527751698	0.0
46-47	4.5153776831220824E-4	0.0	0.0	1.724159340152808	0.0
48-49	4.5153776831220824E-4	0.0	0.0	2.111371790554206	0.0
50-51	4.5153776831220824E-4	0.0	0.0	2.524077310791564	0.0
52-53	4.5153776831220824E-4	0.0	0.0	2.9195491395383404	0.0
54-55	4.5153776831220824E-4	0.0	0.0	3.348735788319094	0.0
56-57	4.5153776831220824E-4	0.0	0.0	3.845314449020445	0.0
58-59	4.5153776831220824E-4	0.0	0.0	4.404995512843428	0.0
60-61	5.079799893512343E-4	0.0	0.0	5.020065209579374	0.0
62-63	5.267940630309096E-4	0.0	0.0	5.618935988877119	0.0
64-65	5.267940630309096E-4	0.0	0.0	6.220497180711059	0.0
66-67	5.267940630309096E-4	0.0	0.0	6.9077752922296	0.0
68-69	5.267940630309096E-4	0.0	0.0	7.693639149829639	0.0
70-71	5.267940630309096E-4	0.0	0.0	8.569584792207962	0.0
72-73	5.267940630309096E-4	0.0	0.0	9.430178150463673	0.0
74-75	5.267940630309096E-4	0.0	0.0	10.259728287147919	0.0
76-77	5.267940630309096E-4	0.0	0.0	11.162051260825148	0.0
78-79	5.267940630309096E-4	0.0	0.0	12.178839058769523	0.0
80-81	5.267940630309096E-4	0.0	0.0	13.253687088089375	3.762814735935069E-5
82-83	5.45608136710585E-4	0.0	0.0	14.38516547918505	3.762814735935069E-5
84-85	5.644222103902603E-4	0.0	0.0	15.45950552851555	3.762814735935069E-5
86-87	5.644222103902603E-4	0.0	0.0	16.558115732892833	3.762814735935069E-5
88-89	5.644222103902603E-4	0.0	0.0	17.733430915662154	3.762814735935069E-5
90-91	6.02050357749611E-4	0.0	0.0	18.995478978094773	3.762814735935069E-5
92-93	6.02050357749611E-4	0.0	0.0	20.29327378051878	3.762814735935069E-5
94-95	6.02050357749611E-4	0.0	0.0	21.550392555647328	3.762814735935069E-5
96-97	6.02050357749611E-4	0.0	0.0	22.789750845222258	3.762814735935069E-5
98-99	6.02050357749611E-4	0.0	0.0	24.092192723845145	3.762814735935069E-5
100-101	6.02050357749611E-4	0.0	0.0	25.506427828272663	3.762814735935069E-5
102-103	6.02050357749611E-4	1.8814073679675344E-5	0.0	26.91936476161628	3.762814735935069E-5
104-105	6.396785051089617E-4	3.762814735935069E-5	0.0	28.263893723060598	3.762814735935069E-5
106-107	6.396785051089617E-4	3.762814735935069E-5	0.0	29.558847600358973	3.762814735935069E-5
108-109	6.396785051089617E-4	5.644222103902603E-5	0.0	30.867347610706716	3.762814735935069E-5
110-111	6.396785051089617E-4	7.525629471870137E-5	0.0	32.23189474654621	3.762814735935069E-5
112-113	6.396785051089617E-4	7.525629471870137E-5	0.0	33.62863276245162	3.762814735935069E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12165	0.0	30.983618	1
GTACATG	17865	0.0	30.545359	1
TACATGG	17830	0.0	29.373075	2
ACATGGG	18630	0.0	27.121962	3
AACGCAG	13345	0.0	27.067446	6
CATGGGG	8665	0.0	26.924257	4
TATCAAC	14255	0.0	25.968164	2
GGTATCA	8890	0.0	25.961893	1
TCAACGC	13945	0.0	25.948446	4
CAACGCA	14555	0.0	24.983614	5
ATCAACG	14525	0.0	24.912292	3
CGCAGAG	14750	0.0	24.325031	8
ACGCAGA	14900	0.0	24.19995	7
TATCCCG	185	2.6869719E-5	22.51918	5
GCAGAGT	15470	0.0	22.269802	9
GAGTACT	4255	0.0	22.162825	12-13
GTATAGA	815	0.0	21.952845	1
ATGGGAG	2270	0.0	20.450079	5
AGTACTT	4615	0.0	20.305058	12-13
TAAGGCG	1000	0.0	20.235094	5
>>END_MODULE