FastQCFastQC Report
Mon 15 Aug 2016
SRR2921105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657585
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135070.5082433863827497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109810.41319468615302996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75780.2851461006891595No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT34890.13128460613677456No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32160.12101212190767181No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30460.11461533685658219No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.10885823031060154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102850.046.9225731
GTATCAA163600.042.5889661
TATCAAC207650.033.407262
TCAACGC207100.033.3233224
ATCAACG207450.033.295833
CAACGCA210350.032.808465
AACGCAG211700.032.7311336
CATGGGG76650.029.312244
GTACATG172100.029.2202721
TACATGG167500.029.2111192
ACGCAGA239400.028.8011787
CGCAGAG241000.028.585288
ACATGGG169600.028.2871933
GCAGAGT252750.026.5723049
CGCTATA900.00941203226.5127681
GAGTACT152350.026.0253412-13
AGTACTT156950.025.31942612-13
GTACTTT169850.023.39797414-15
TATACCG1802.1513488E-523.1763695
AGAGTAC242200.023.11223410-11
Produced by FastQC (version 0.11.5)