FastQCFastQC Report
Mon 15 Aug 2016
SRR2921104_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921104_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798290
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT53340.1111646023896013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114150.037.7838131
GGTATCA75650.037.377721
TCAACGC133500.031.5670154
TACATGG204000.031.4237522
AACGCAG135100.031.4091956
CAACGCA139450.030.305185
ATCAACG139150.030.2421883
ACATGGG207800.030.104693
GTACATG223150.030.1012761
TATCAAC143250.029.7087142
CATGGGG134900.029.6068444
ACGCAGA156500.026.8461067
CGCAGAG160600.025.7896548
GTATAAT38450.023.4273951
ATGGGGA65150.023.0230645
GCAGAGT177850.022.6190669
GTGTAGC43450.020.3196091
TATACCG3251.45118975E-820.1458845
AGAGTAC157850.019.75172810-11
TATAATG44000.019.7502522
Produced by FastQC (version 0.11.5)