FastQCFastQC Report
Mon 15 Aug 2016
SRR2921104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798290
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116080.24191951716132204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101790.21213807418892983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64400.13421448057537164No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT52380.10916388963568271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94800.058.4540981
GTATCAA166100.047.9064251
TCAACGC210900.037.3845064
ATCAACG214450.036.7934343
CAACGCA215700.036.6634835
TATCAAC217550.036.461282
AACGCAG221550.035.7857976
ACGCAGA252350.031.2127467
TACATGG198600.030.4278772
CGCAGAG258150.030.3968818
ACATGGG207450.028.5262183
GTACATG216650.028.4688171
CATGGGG133500.027.944834
GCAGAGT284150.026.8191059
GAGTACT161450.024.98293912-13
AGAGTAC255650.024.52661510-11
CAGAGTA266450.024.02366810-11
AGTACTT169950.023.89094512-13
GTATAAT39300.023.8294851
GTACTTT175800.023.01265714-15
Produced by FastQC (version 0.11.5)