##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4798290
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405412553222085	33.0	31.0	34.0	28.0	34.0
2	31.48497214632713	33.0	31.0	34.0	28.0	34.0
3	31.579402662198408	34.0	31.0	34.0	28.0	34.0
4	35.05132015780622	37.0	35.0	37.0	32.0	37.0
5	34.81893924710678	37.0	35.0	37.0	32.0	37.0
6	34.572734870130816	37.0	35.0	37.0	31.0	37.0
7	34.52000295938762	37.0	35.0	37.0	31.0	37.0
8	34.51914702946258	37.0	35.0	37.0	31.0	37.0
9	36.09260965885763	39.0	37.0	39.0	31.0	39.0
10-11	36.0542416152421	39.0	37.0	39.0	31.0	39.0
12-13	35.999900485381254	39.0	37.0	39.0	30.0	39.0
14-15	37.26823410006482	40.0	38.0	41.0	31.0	41.0
16-17	37.266195248724024	40.0	38.0	41.0	31.0	41.0
18-19	37.292178359373864	40.0	38.0	41.0	31.0	41.0
20-21	37.30302232670388	40.0	38.0	41.0	31.0	41.0
22-23	37.26100141925561	40.0	38.0	41.0	31.0	41.0
24-25	37.21506765952037	40.0	37.5	41.0	31.0	41.0
26-27	37.10699478355831	40.0	37.0	41.0	30.0	41.0
28-29	36.986645033960016	40.0	37.0	41.0	30.0	41.0
30-31	36.851653401524295	40.0	37.0	41.0	30.0	41.0
32-33	36.71996461239317	40.0	36.5	41.0	30.0	41.0
34-35	36.57037236182056	40.0	36.0	41.0	30.0	41.0
36-37	36.395684504271316	40.0	36.0	41.0	29.5	41.0
38-39	36.25551206784083	40.0	36.0	41.0	29.0	41.0
40-41	36.12840345206313	40.0	35.0	41.0	28.0	41.0
42-43	35.96327024835931	40.0	35.0	41.0	27.5	41.0
44-45	35.775196997263606	40.0	35.0	41.0	27.0	41.0
46-47	35.494199912885634	40.0	35.0	41.0	25.5	41.0
48-49	35.221015299200346	40.0	35.0	41.0	24.0	41.0
50-51	35.0218836293763	39.0	35.0	41.0	24.0	41.0
52-53	34.81812531130882	39.0	35.0	41.0	23.0	41.0
54-55	34.60611540778069	39.0	34.5	41.0	23.0	41.0
56-57	34.36960761021113	38.5	34.0	41.0	22.0	41.0
58-59	34.12653539073295	38.0	34.0	40.5	22.0	41.0
60-61	33.85502762442454	37.5	34.0	40.0	20.5	41.0
62-63	33.57665147375419	37.0	33.5	40.0	20.0	41.0
64-65	33.27887778771187	37.0	33.0	40.0	19.5	41.0
66-67	32.95475742399897	36.0	33.0	39.5	18.0	41.0
68-69	32.60276358869514	36.0	33.0	39.0	17.0	41.0
70-71	32.23388144526487	35.0	32.0	39.0	13.5	40.5
72-73	31.864200788197465	35.0	32.0	37.5	11.0	40.0
74-75	31.551151972890345	35.0	32.0	37.0	9.0	39.0
76-77	31.25332170419045	35.0	32.0	37.0	7.5	39.0
78-79	30.95124731935752	35.0	31.5	36.0	7.0	39.0
80-81	30.909874142663327	35.0	32.0	36.0	7.0	37.5
82-83	30.702009361668427	35.0	32.0	35.5	4.0	37.0
84-85	30.493778825373205	35.0	32.0	35.0	2.0	37.0
86-87	30.315744984150605	35.0	32.0	35.0	2.0	36.0
88-89	30.135414595616353	35.0	31.0	35.0	2.0	36.0
90-91	29.984548036904812	35.0	31.0	35.0	2.0	36.0
92-93	29.876755260728302	35.0	31.0	35.0	2.0	35.5
94-95	29.76939753537198	35.0	31.0	35.0	2.0	35.0
96-97	29.70361587148755	35.0	31.0	35.0	2.0	35.0
98-99	29.61135373226712	35.0	31.0	35.0	2.0	35.0
100-101	29.518423021534755	35.0	31.0	35.0	2.0	35.0
102-103	28.209170767085773	33.0	28.0	34.5	2.0	35.0
104-105	29.21781957739111	34.0	30.5	35.0	2.0	35.0
106-107	29.382019010939317	35.0	31.0	35.0	2.0	35.0
108-109	29.38226243099104	35.0	31.0	35.0	2.0	35.0
110-111	29.294403943905017	35.0	31.0	35.0	2.0	35.0
112-113	29.189068814098356	35.0	31.0	35.0	2.0	35.0
114-115	29.096527825537848	35.0	31.0	35.0	2.0	35.0
116-117	28.991894195640533	35.0	30.5	35.0	2.0	35.0
118-119	28.848917218425733	34.0	30.0	35.0	2.0	35.0
120-121	28.97798997142732	35.0	31.0	35.0	2.0	35.0
122-123	28.883458586288032	35.0	31.0	35.0	2.0	35.0
124-125	28.211886213630272	34.5	29.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75103.0
3	31272.0
4	13528.0
5	11994.0
6	14110.0
7	16723.0
8	18313.0
9	20575.0
10	23710.0
11	26990.0
12	29292.0
13	35074.0
14	45262.0
15	42413.0
16	24216.0
17	19930.0
18	18575.0
19	17972.0
20	17538.0
21	19025.0
22	19852.0
23	21665.0
24	28170.0
25	37384.0
26	46108.0
27	53933.0
28	64895.0
29	77808.0
30	93640.0
31	114525.0
32	143796.0
33	185837.0
34	254566.0
35	389625.0
36	755699.0
37	1389686.0
38	597511.0
39	1975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.928731642930934	18.37250600516819	12.605060435778883	25.09370191612199
2	16.098046469941483	20.552365965118884	38.49426551125794	24.855322053681693
3	18.65483328435755	24.736437355808004	30.323135950515706	26.285593409318736
4	12.681255322457009	16.51409130262068	36.96543565678338	33.83921771813893
5	13.130130498018708	37.991955450064665	34.758420366447965	14.119493685468656
6	32.16262634794605	37.031906473582296	16.84687249402738	13.958594684444279
7	27.717920746391034	32.84531569344511	21.27505828574668	18.161705274417173
8	26.424575206774183	34.61996529203505	19.882690746863215	19.072768754327555
9	26.716198620550507	14.749867176243004	19.45692435413471	39.07700984907178
10-11	25.160857283033373	25.723857773650167	28.026463711655715	21.08882123166075
12-13	25.9039074496816	23.324226771624	27.982277376872528	22.789588401821867
14-15	23.18027621543933	24.60661430104442	25.93514592963083	26.27796355388542
16-17	22.677047709986116	27.423606972749447	26.532222108970938	23.367123208293496
18-19	22.58302749936606	26.195722830369732	28.73567075843037	22.485578911833834
20-21	23.952600965328212	25.494001380171905	28.33840414314223	22.214993511357655
22-23	23.2747576910822	25.389261351134806	28.21507565825028	23.12090529953271
24-25	22.937676212314667	25.46092711780494	28.61238762800636	22.989009041874038
26-27	22.750620903830026	25.664837941729207	28.48332286029854	23.101218294142225
28-29	22.730785582061465	25.89715018743295	28.51084111520287	22.861223115302714
30-31	23.12830180891653	25.603432515887974	28.326062743932322	22.94220293126317
32-33	22.83197743003116	25.795786725134462	28.282366836620998	23.08986900821338
34-35	23.038809764002526	25.847605708581835	28.280268200411758	22.833316327003885
36-37	23.203799819289497	25.462253778290993	28.254657349934355	23.079289052485155
38-39	22.865449097078308	25.839377199356488	28.20674044789852	23.088433255666686
40-41	23.111689426002783	25.74004930917335	28.21407569064424	22.934185574179622
42-43	23.19689764994294	25.633160050624664	28.053965732360293	23.115976567072103
44-45	23.097348278234666	25.868596038099472	27.82627068160879	23.207785002057076
46-47	23.15263290759793	25.799767818589146	27.483176853735507	23.564422420077417
48-49	23.09491098714255	25.834156165782137	27.522322782526476	23.548610064548832
50-51	22.92113566312586	26.271211611965263	27.31092126072468	23.496731464184194
52-53	23.27087967610364	26.164221408108745	27.071407191619784	23.493491724167832
54-55	23.132880531555895	26.00203211551271	27.158268113973143	23.706819238958253
56-57	23.015268468353923	26.330507807757265	26.91824561385225	23.735978110036566
58-59	22.887604743814585	26.083987228768702	27.234548606841084	23.79385942057562
60-61	22.93958214261446	26.1727304220804	27.239458203630956	23.648229231674183
62-63	22.706704292263215	26.146096799444642	27.25919025900444	23.888008649287702
64-65	22.646790340074425	26.045397872921892	27.313143761737567	23.994668025266115
66-67	22.67567007857332	26.237238701691858	27.091999329215312	23.995091890519518
68-69	22.953266504594016	25.895212381110998	27.14993443374035	24.001586680554638
70-71	22.645348982644673	25.609362570729584	27.390526529951988	24.354761916673752
72-73	22.739378543104507	25.64195813321734	27.38371643969221	24.234946883985938
74-75	22.483703617085183	25.983749578168965	27.062699047448724	24.46984775729713
76-77	22.314670037028684	25.787073183237176	27.274244034163264	24.624012745570877
78-79	22.55596682051932	25.95903964929599	27.026996888485726	24.45799664169896
80-81	22.42028172889326	25.760434557854893	26.960304490653503	24.858979222598343
82-83	22.42205445245311	25.688221978580867	27.12375822810667	24.76596534085935
84-85	22.583793561146102	25.56779147344602	26.899513175688632	24.948901789719244
86-87	22.07824852430636	25.501057405449153	27.103626150872213	25.317067919372278
88-89	22.18408413757351	25.598522595937933	26.981301174913103	25.236092091575458
90-91	22.04502115216038	25.55960129655015	27.066907027940907	25.32847052334856
92-93	22.114364771877533	25.43605510061046	27.001143804047345	25.44843632346466
94-95	21.84708992128433	25.490477458921575	27.023730171533717	25.638702448260382
96-97	21.808268982856514	25.296999194244584	27.205589989413976	25.68914183348493
98-99	21.778536724468722	25.314573305106297	26.94488890815902	25.962001062265962
100-101	21.674378637640622	25.46128245909744	26.917073545967835	25.947265357294107
102-103	21.64601506492727	25.137690083395732	27.037846585877922	26.17844826579908
104-105	21.431401664387092	25.202410247281914	26.971358251755746	26.394829836575244
106-107	21.309932919383094	25.275488273789552	26.904545792320544	26.510033014506813
108-109	21.229853366859643	25.25491764599952	26.917452578502253	26.597776408638584
110-111	21.13876140860525	25.418593462596117	26.73407464600597	26.708570482792666
112-113	21.131346713531233	25.54185111555207	26.690936188285075	26.635865982631625
114-115	20.985721165505396	25.48370358227195	26.680153002623864	26.850422249598793
116-117	20.83550534528581	25.44990088122025	26.841919980057437	26.8726737934365
118-119	20.845846015357967	25.441554342147725	26.649097414310297	27.06350222818401
120-121	20.764784798049067	25.63366993037483	26.647129157434758	26.95441611414135
122-123	20.719801427477083	25.55212325518797	26.450113905756	27.277961411578943
124-125	20.549138417201895	25.595246778937874	26.565118786760706	27.290496017099525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	135.5
2	278.5
3	527.5
4	428.5
5	290.5
6	539.0
7	1161.5
8	1857.0
9	2162.5
10	1937.0
11	1651.0
12	1725.0
13	2028.5
14	2373.0
15	2624.0
16	2970.0
17	3381.0
18	3883.5
19	4474.0
20	5204.0
21	6089.5
22	7402.5
23	9413.5
24	11759.5
25	14790.0
26	20333.0
27	27853.5
28	33130.5
29	39082.5
30	44765.0
31	49967.0
32	58490.0
33	67157.5
34	77664.0
35	87951.0
36	101056.5
37	111465.5
38	115419.5
39	120176.5
40	128541.5
41	136286.5
42	139911.5
43	149659.5
44	160595.5
45	166715.0
46	173471.0
47	180117.0
48	185106.0
49	189749.0
50	192587.5
51	191799.5
52	186683.5
53	182568.5
54	177699.5
55	168092.5
56	155971.0
57	143122.5
58	134850.0
59	125400.0
60	108565.5
61	89172.0
62	71392.5
63	54120.0
64	40256.0
65	30912.0
66	22511.0
67	16687.0
68	12911.5
69	9605.5
70	7321.0
71	5517.5
72	4129.0
73	3563.5
74	2835.5
75	2042.0
76	1401.5
77	876.0
78	553.5
79	343.0
80	232.0
81	184.5
82	140.5
83	70.5
84	49.0
85	47.5
86	38.0
87	30.0
88	20.5
89	14.0
90	11.5
91	11.5
92	11.5
93	8.5
94	9.0
95	22.0
96	29.5
97	22.5
98	12.5
99	3.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16324565626504442
2	0.011775028187124996
3	0.0
4	0.004668329759143362
5	0.1401124150478608
6	0.07283844869734843
7	0.007836124952847785
8	0.011337372272205307
9	0.012066798797071458
10-11	0.005168507947623007
12-13	0.009743054296426435
14-15	0.011451996440398559
16-17	0.014567689739469685
18-19	0.018517013352673556
20-21	0.022122464461297668
22-23	0.02416485873092289
24-25	0.02427948289911614
26-27	0.02187237536705785
28-29	0.020309318528058954
30-31	0.023112400459330305
32-33	0.02332080803786349
34-35	0.022018260672031078
36-37	0.023227024627523556
38-39	0.01800641478526725
40-41	0.0252381577603688
42-43	0.021330515662871564
44-45	0.029708500319905636
46-47	0.024571253509062602
48-49	0.01744371432322765
50-51	0.013598594499290373
52-53	0.014713575044442916
54-55	0.017224886365767803
56-57	0.013702798288556965
58-59	0.01317135896329734
60-61	0.016005702031348667
62-63	0.01395288738279679
64-65	0.024863024119009064
66-67	0.019246439877539708
68-69	0.01777716644888075
70-71	0.023883508499903092
72-73	0.02885402924791957
74-75	0.01569309066354889
76-77	0.00808621404708761
78-79	0.013046314416177431
80-81	0.016047383547055306
82-83	0.01479693807585619
84-85	0.013879944730310173
86-87	0.009618009749306523
88-89	0.01993418488669922
90-91	0.016453778325195016
92-93	0.014640632391956301
94-95	0.010753831052312387
96-97	0.019350643666806302
98-99	0.02079907633761194
100-101	0.027311813166773995
102-103	0.01921517874075973
104-105	0.012671180774817697
106-107	0.017787586827807407
108-109	0.009711793159646457
110-111	0.02154934362033141
112-113	0.016203689230955193
114-115	0.012389830543797895
116-117	0.010649627263045794
118-119	0.0067628259234018785
120-121	0.0035220880772108398
122-123	0.005095565295136393
124-125	5.835412198929202E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4798290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.64275993771368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.96644081661618	26.810582180551286
2	14.773947346206379	12.00907990648576
3	6.768819973961867	8.253105757900006
4	3.714340552341561	6.038442055829315
5	2.271442897570399	4.615885419908924
6	1.4727911208732185	3.5914977578427876
7	0.9992984511453683	2.8429972939221217
8	0.7374210205212643	2.3976660408055666
9	0.5252840846043011	1.9214095454706863
>10	2.5405450403734298	17.742673094567937
>50	0.1431141050114942	4.002228119501457
>100	0.07828962754046175	5.8152746949520076
>500	0.005544199696431855	1.5671601109797397
>1k	0.0025154228931332967	1.6937833817179373
>5k	1.0267032216855907E-4	0.24365009893430276
>10k+	1.0267032216855907E-4	0.45456454063038654
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11608	0.24191951716132204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10179	0.21213807418892983	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6440	0.13421448057537164	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	5238	0.10916388963568271	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	6.252227355995573E-5
10-11	0.0	0.0	0.0	0.0	8.33630314132743E-5
12-13	0.0	0.0	0.0	0.0	8.33630314132743E-5
14-15	0.0	0.0	0.0	5.210189463329644E-5	1.1462416819325218E-4
16-17	0.0	0.0	0.0	8.33630314132743E-5	1.667260628265486E-4
18-19	0.0	0.0	0.0	1.1462416819325218E-4	1.771464417532079E-4
20-21	0.0	0.0	0.0	1.4588530497323005E-4	1.979871996065265E-4
22-23	0.0	0.0	0.0	3.647132624330751E-4	2.1882795745984507E-4
24-25	0.0	0.0	0.0	7.711080405727874E-4	2.2924833638650436E-4
26-27	0.0	0.0	0.0	0.0016047383547055304	2.2924833638650436E-4
28-29	0.0	0.0	0.0	0.00468917051699668	2.2924833638650436E-4
30-31	0.0	0.0	0.0	0.01596402051564203	2.2924833638650436E-4
32-33	0.0	0.0	0.0	0.04169193608556382	2.2924833638650436E-4
34-35	0.0	0.0	0.0	0.0822897323838284	2.2924833638650436E-4
36-37	0.0	0.0	0.0	0.15060573662700671	2.2924833638650436E-4
38-39	0.0	0.0	0.0	0.2615827721959281	2.2924833638650436E-4
40-41	0.0	0.0	0.0	0.3929733300821751	2.2924833638650436E-4
42-43	0.0	0.0	0.0	0.5176323231818002	2.2924833638650436E-4
44-45	0.0	0.0	0.0	0.6703846578676986	2.3966871531316364E-4
46-47	0.0	0.0	0.0	0.8601918600167977	2.6050947316648226E-4
48-49	0.0	0.0	0.0	1.0679221139197506	2.917706099464601E-4
50-51	0.0	0.0	0.0	1.2819775378311857	2.917706099464601E-4
52-53	0.0	0.0	0.0	1.498346285864339	3.438725045797565E-4
54-55	0.0	0.0	0.0	1.7437357892082388	3.9597439921305297E-4
56-57	0.0	0.0	0.0	2.0332034954119074	3.9597439921305297E-4
58-59	0.0	0.0	0.0	2.3574544264727644	3.9597439921305297E-4
60-61	0.0	0.0	0.0	2.7067663688522368	3.9597439921305297E-4
62-63	0.0	0.0	0.0	3.0664257475058823	4.584966727730087E-4
64-65	0.0	0.0	0.0	3.4438935537451885	4.584966727730087E-4
66-67	2.0840757853318577E-5	0.0	0.0	3.884529697037903	4.68917051699668E-4
68-69	2.0840757853318577E-5	0.0	0.0	4.381581771839551	4.793374306263273E-4
70-71	2.0840757853318577E-5	0.0	0.0	4.9313609640100955	4.897578095529866E-4
72-73	2.0840757853318577E-5	0.0	0.0	5.4801502201826064	5.105985674063051E-4
74-75	2.0840757853318577E-5	0.0	0.0	6.038151091326285	5.210189463329644E-4
76-77	2.0840757853318577E-5	0.0	0.0	6.65236990677929	5.210189463329644E-4
78-79	2.0840757853318577E-5	0.0	0.0	7.337937056743131	5.210189463329644E-4
80-81	2.0840757853318577E-5	0.0	0.0	8.08402576751301	5.418597041862831E-4
82-83	2.0840757853318577E-5	0.0	0.0	8.868774084100794	5.627004620396016E-4
84-85	2.0840757853318577E-5	0.0	0.0	9.63452604990528	5.627004620396016E-4
86-87	2.0840757853318577E-5	0.0	0.0	10.435300909282265	5.731208409662609E-4
88-89	2.0840757853318577E-5	0.0	0.0	11.307455364306868	5.835412198929202E-4
90-91	2.0840757853318577E-5	0.0	0.0	12.23916228489733	5.835412198929202E-4
92-93	2.0840757853318577E-5	0.0	0.0	13.196951413941218	6.043819777462388E-4
94-95	2.0840757853318577E-5	0.0	0.0	14.154844746774371	6.460634934528759E-4
96-97	2.0840757853318577E-5	0.0	0.0	15.121480777526994	6.564838723795352E-4
98-99	2.0840757853318577E-5	0.0	0.0	16.142636230823896	6.669042513061945E-4
100-101	2.0840757853318577E-5	0.0	0.0	17.23428554755965	6.669042513061945E-4
102-103	2.0840757853318577E-5	0.0	0.0	18.359853197701682	6.773246302328537E-4
104-105	2.0840757853318577E-5	0.0	0.0	19.458921824233215	6.877450091595131E-4
106-107	2.0840757853318577E-5	0.0	0.0	20.538504342171898	6.981653880861724E-4
108-109	2.0840757853318577E-5	0.0	0.0	21.627798653270226	7.085857670128317E-4
110-111	2.0840757853318577E-5	0.0	0.0	22.76883014573942	7.085857670128317E-4
112-113	2.0840757853318577E-5	0.0	0.0	23.94820863265872	7.085857670128317E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9480	0.0	58.454098	1
GTATCAA	16610	0.0	47.906425	1
TCAACGC	21090	0.0	37.384506	4
ATCAACG	21445	0.0	36.793434	3
CAACGCA	21570	0.0	36.663483	5
TATCAAC	21755	0.0	36.46128	2
AACGCAG	22155	0.0	35.785797	6
ACGCAGA	25235	0.0	31.212746	7
TACATGG	19860	0.0	30.427877	2
CGCAGAG	25815	0.0	30.396881	8
ACATGGG	20745	0.0	28.526218	3
GTACATG	21665	0.0	28.468817	1
CATGGGG	13350	0.0	27.94483	4
GCAGAGT	28415	0.0	26.819105	9
GAGTACT	16145	0.0	24.982939	12-13
AGAGTAC	25565	0.0	24.526615	10-11
CAGAGTA	26645	0.0	24.023668	10-11
AGTACTT	16995	0.0	23.890945	12-13
GTATAAT	3930	0.0	23.829485	1
GTACTTT	17580	0.0	23.012657	14-15
>>END_MODULE