FastQCFastQC Report
Mon 15 Aug 2016
SRR2921103_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921103_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096849
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93050.031.9898911
GTACATG152950.027.846931
TCAACGC103500.027.6619054
AACGCAG104500.027.2802816
GGTATCA68750.027.1581521
ATCAACG107900.026.864883
CAACGCA107150.026.664075
TACATGG154400.026.6374242
CATGGGG93250.025.979034
ACATGGG157100.025.6501483
TATCAAC115500.025.5084862
ACGCAGA117900.024.0268177
CGCAGAG123900.022.863578
GTATAGG10750.020.5315571
TACACCG5550.019.3044285
GCAGAGT141150.019.2683129
AGAGTAC121600.017.63953810-11
GTACCGT7350.017.0044126
ATGGGGA56400.016.885675
CAGAGTA132350.016.54395710-11
Produced by FastQC (version 0.11.5)