##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921103_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4096849
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.54411426928354	31.0	30.0	34.0	24.0	34.0
2	29.652781930698445	31.0	30.0	34.0	25.0	34.0
3	29.82532307146297	31.0	30.0	34.0	25.0	34.0
4	33.23576363200108	37.0	35.0	37.0	26.0	37.0
5	33.28131205226261	37.0	35.0	37.0	27.0	37.0
6	33.273187515576	37.0	35.0	37.0	26.0	37.0
7	33.12396307503645	37.0	35.0	37.0	26.0	37.0
8	33.09546727253067	37.0	35.0	37.0	26.0	37.0
9	34.51948460878104	39.0	35.0	39.0	26.0	39.0
10-11	34.513656104972384	39.0	35.0	39.0	26.0	39.0
12-13	34.45675969507297	39.0	35.0	39.0	25.5	39.0
14-15	35.62757048160672	40.0	36.0	41.0	25.5	41.0
16-17	35.58706862273908	40.0	36.0	41.0	25.0	41.0
18-19	35.60233364715175	40.0	36.0	41.0	25.0	41.0
20-21	35.65914291691004	40.0	36.0	41.0	25.5	41.0
22-23	35.56496456178883	40.0	36.0	41.0	25.0	41.0
24-25	35.542775069327675	40.0	36.0	41.0	25.0	41.0
26-27	35.481364092257245	40.0	36.0	41.0	25.0	41.0
28-29	35.35324038059494	40.0	35.0	41.0	25.0	41.0
30-31	35.25047835543853	40.0	35.0	41.0	24.0	41.0
32-33	35.14014783068646	40.0	35.0	41.0	24.0	41.0
34-35	35.03279544840437	40.0	35.0	41.0	24.0	41.0
36-37	34.90013276056794	40.0	35.0	41.0	23.5	41.0
38-39	34.75921226288789	40.0	35.0	41.0	22.5	41.0
40-41	34.634429045346806	39.0	34.0	41.0	21.5	41.0
42-43	34.425619421169785	39.0	34.0	41.0	19.5	41.0
44-45	34.21121781642428	39.0	34.0	41.0	18.0	41.0
46-47	34.026133255094344	39.0	33.0	41.0	17.0	41.0
48-49	33.78614601123937	38.0	33.0	40.0	14.5	41.0
50-51	33.61072924581795	38.0	33.0	40.0	13.5	41.0
52-53	33.42350059765444	38.0	33.0	40.0	10.5	41.0
54-55	33.193376665822925	38.0	32.5	40.0	8.5	41.0
56-57	32.94529625085035	38.0	32.0	40.0	7.5	41.0
58-59	32.663545446756764	37.0	32.0	40.0	6.5	41.0
60-61	32.3595086125947	37.0	31.5	40.0	2.0	41.0
62-63	32.07053750333488	36.0	31.0	39.5	2.0	41.0
64-65	31.747916874651715	36.0	31.0	39.0	2.0	41.0
66-67	31.39982349849848	35.0	31.0	39.0	2.0	40.5
68-69	31.028916247584426	35.0	30.0	38.5	2.0	40.0
70-71	30.670204466896386	35.0	30.0	37.5	2.0	40.0
72-73	30.309481994576807	35.0	30.0	37.0	2.0	39.0
74-75	30.267926154954697	35.0	30.0	37.0	2.0	39.0
76-77	30.316968357876995	35.0	30.5	36.0	2.0	39.0
78-79	30.140382401206388	35.0	31.0	36.0	2.0	38.5
80-81	29.912969699395802	35.0	30.5	36.0	2.0	37.0
82-83	29.667901355407537	35.0	30.0	35.0	2.0	37.0
84-85	29.421886918458554	35.0	30.0	35.0	2.0	36.5
86-87	29.24975499463124	35.0	30.0	35.0	2.0	36.0
88-89	29.061768813056084	34.5	30.0	35.0	2.0	36.0
90-91	28.90788603631718	34.0	30.0	35.0	2.0	35.5
92-93	28.789375932576476	34.0	30.0	35.0	2.0	35.0
94-95	28.672363199131823	34.0	29.0	35.0	2.0	35.0
96-97	28.571264159357593	34.0	29.0	35.0	2.0	35.0
98-99	28.4516364894093	34.0	29.0	35.0	2.0	35.0
100-101	28.316903185838676	34.0	29.0	35.0	2.0	35.0
102-103	26.978136733865465	32.5	26.0	34.0	2.0	35.0
104-105	27.954722885808096	34.0	29.0	35.0	2.0	35.0
106-107	28.09925274277866	34.0	29.0	35.0	2.0	35.0
108-109	28.029851478538752	34.0	29.0	35.0	2.0	35.0
110-111	27.919974351019526	34.0	29.0	35.0	2.0	35.0
112-113	27.789263285027104	34.0	29.0	35.0	2.0	35.0
114-115	27.644309931852504	34.0	28.5	35.0	2.0	35.0
116-117	27.486954730330552	34.0	27.5	35.0	2.0	35.0
118-119	27.268134729886313	34.0	27.0	35.0	2.0	35.0
120-121	27.041869495312127	34.0	27.0	35.0	2.0	35.0
122-123	26.727196438042995	34.0	25.5	35.0	2.0	35.0
124-125	25.423081250980935	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	170420.0
3	25413.0
4	16752.0
5	16859.0
6	21489.0
7	21804.0
8	20174.0
9	21679.0
10	23393.0
11	24592.0
12	25182.0
13	24716.0
14	24760.0
15	22292.0
16	18378.0
17	16739.0
18	16033.0
19	15752.0
20	16300.0
21	18014.0
22	21186.0
23	27246.0
24	36412.0
25	45897.0
26	51106.0
27	57756.0
28	66864.0
29	77699.0
30	92202.0
31	111557.0
32	139801.0
33	180043.0
34	245889.0
35	370999.0
36	681710.0
37	1033088.0
38	296424.0
39	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.168343245955406	17.804831485424774	11.754803338473211	25.27202193014661
2	16.367089711388548	20.419555105688715	37.72798629035154	25.4853688925712
3	19.140049134047228	24.229065204842858	28.86025572471254	27.770629936397373
4	12.899304554644297	16.50339123662633	35.719991635503376	34.877312573225986
5	13.950893566079914	38.039627181999194	33.788646885650415	14.220832366270475
6	33.38264181708076	36.124015927951774	16.68343953434153	13.809902720625935
7	28.83982350340895	32.377012826718946	20.60700576226133	18.176157907610772
8	26.21702867598618	35.62344710024377	19.479178680971103	18.680345542798943
9	26.269057489421062	16.20669818762628	18.71416869415448	38.81007562879818
10-11	25.060494855738334	26.37404682731846	27.448587923995397	21.116870392947803
12-13	25.628560183887917	24.031230555413728	27.10345991792079	23.236749342777564
14-15	23.123941015720916	25.372292145894594	24.31288641091827	27.19088042746622
16-17	23.43145881809385	27.88551998273095	25.2618363415514	23.421184857623796
18-19	23.407184146807136	27.253532499089346	26.474876545456898	22.864406808646617
20-21	24.27631971121194	26.463142680964047	26.178295259212497	23.08224234861152
22-23	24.03469308544276	26.177668695592065	25.917150570951282	23.870487648013892
24-25	23.691016596628494	26.410205297104028	26.074965029771718	23.823813076495757
26-27	23.610544426272526	26.52176664290255	26.1152072584306	23.752481672394325
28-29	23.598874685168	26.70091732629867	26.1091876891603	23.59102029937303
30-31	23.848773409291894	26.614050216687108	25.856561441909776	23.680614932111222
32-33	23.50145434009753	26.697044668048754	25.912210410048047	23.88929058180567
34-35	23.782343876271696	26.628309387211864	26.04267745097177	23.54666928554467
36-37	23.876859980312002	26.51360808666433	25.884246827756147	23.725285105267524
38-39	23.649081246025954	26.690130884853524	25.969862358506905	23.690925510613617
40-41	23.774356576443598	26.61005218529113	26.061655891739154	23.553935346526114
42-43	23.76205904028934	26.507097590866774	25.945431445731543	23.785411923112342
44-45	23.66135145678309	26.67137942801505	25.928325641712725	23.73894347348913
46-47	23.61599552150604	26.62677885860687	25.907474500416257	23.849751119470834
48-49	23.58414928663241	26.575487511105816	25.96322180190577	23.877141400356
50-51	23.467442425663553	26.74188549005814	25.88989400484361	23.900778079434694
52-53	23.663736222932602	26.60426893050529	25.763561852247598	23.968432994314508
54-55	23.575016056346353	26.555193644789398	25.83565679068021	24.034133508184034
56-57	23.540317518022142	26.5639863081454	25.805280875172443	24.090415298660023
58-59	23.484706378887886	26.261840979166912	25.99910704167009	24.25434560027512
60-61	23.45883034348588	26.307323120082344	26.062892998796784	24.170953537634997
62-63	23.354914110720955	26.288467441519302	26.08314814613496	24.27347030162478
64-65	23.343767977165108	26.225231970315615	26.05238248201215	24.378617570507128
66-67	23.34847843843797	26.26898165879815	26.002759680621562	24.379780222142323
68-69	23.47722766859518	26.03892563412816	26.139422696408975	24.344424000867683
70-71	23.201934674210754	25.956378226365434	26.285553760797807	24.556133338626
72-73	23.291761175672082	25.96538399822001	26.23608253893665	24.506772287171255
74-75	23.211743473388157	25.97374391923461	26.30972891924988	24.504783688127354
76-77	23.09477539386375	25.771545162053588	26.58594495233984	24.547734491742826
78-79	23.125210070075468	25.803664599186295	26.53577102557188	24.535354305166358
80-81	23.056050897374526	25.77192604656236	26.586869369537187	24.585153686525928
82-83	23.025525175209058	25.76003731017848	26.632301985731154	24.582135528881306
84-85	23.045707403768503	25.749213037563234	26.52824141243159	24.676838146236673
86-87	22.968956459308288	25.644247702435013	26.65481964044613	24.731976197810567
88-89	23.022475957869027	25.5480995267898	26.594376431079226	24.835048084261945
90-91	22.95903896661648	25.612634596879342	26.6182223354661	24.810104101038082
92-93	22.990991096608155	25.502056070840258	26.693518379098357	24.813434453453226
94-95	22.714884427131622	25.461491386544616	26.81751054465768	25.00611364166609
96-97	22.813975771469888	25.353837059257117	26.809014522827912	25.02317264644508
98-99	22.717110867527488	25.469493186613214	26.691854163295343	25.12154178256396
100-101	22.625090955418806	25.571002148239653	26.75466546178602	25.049241434555526
102-103	22.442282028687043	25.5701823372066	26.873342855300614	25.114192778805744
104-105	22.261006813913507	25.719940509847895	26.74411589490594	25.274936781332656
106-107	22.172986593884037	25.754424580138963	26.780816019670368	25.291772806306632
108-109	22.201072065775126	25.705905303381034	26.837374203530278	25.255648427313567
110-111	22.219136051280923	25.948386844606002	26.72897230507655	25.10350479903653
112-113	22.180104013262657	25.909603090060067	26.793747577188604	25.116545319488676
114-115	22.096217450514718	25.974482842777096	26.744375377363873	25.184924329344316
116-117	21.973041972844104	25.925408861539033	26.82640780550627	25.27514136011059
118-119	22.06731175557035	25.8673745806572	26.75168897380643	25.313624689966012
120-121	22.034945040794707	25.986258457741013	26.783545969806923	25.195250531657358
122-123	22.021202138535784	26.057547963761664	26.773935465273226	25.14731443242933
124-125	22.0710752797863	26.015639078270524	26.605784907391516	25.307500734551663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3197.0
1	2244.5
2	1059.0
3	743.0
4	608.0
5	541.5
6	509.0
7	510.0
8	564.0
9	608.5
10	590.0
11	612.5
12	700.0
13	822.5
14	953.0
15	1117.0
16	1332.5
17	1640.5
18	2021.0
19	2426.5
20	2978.5
21	3676.5
22	4578.5
23	5849.0
24	7364.5
25	9231.5
26	12241.5
27	15935.0
28	19524.0
29	23742.0
30	28992.5
31	34779.5
32	42221.0
33	49658.5
34	55973.0
35	62618.0
36	70786.5
37	79597.5
38	86802.5
39	94543.5
40	102354.5
41	110812.5
42	120383.5
43	130183.0
44	139605.5
45	149172.0
46	157733.5
47	164112.0
48	169622.5
49	174524.5
50	178897.5
51	178627.5
52	173722.5
53	170075.0
54	164762.0
55	154555.5
56	143743.0
57	131672.5
58	119864.0
59	106275.5
60	89921.5
61	75443.5
62	62145.5
63	48067.0
64	36012.0
65	27643.0
66	20852.0
67	15454.5
68	11510.0
69	8773.0
70	6749.0
71	5159.5
72	3957.0
73	3233.0
74	2516.0
75	1917.5
76	1375.0
77	829.5
78	535.0
79	392.0
80	281.5
81	225.0
82	171.0
83	114.0
84	92.5
85	82.0
86	58.5
87	44.5
88	44.0
89	34.5
90	23.5
91	20.0
92	26.5
93	22.5
94	12.0
95	11.5
96	16.5
97	18.0
98	13.0
99	14.5
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2721359757218292
2	0.06829639071393649
3	0.07688835981018582
4	0.08194102345485518
5	0.09028890251996108
6	0.120165522331919
7	0.11062160211421021
8	0.1099625590301229
9	0.11611362781493778
10-11	0.0961470632674038
12-13	0.08508978485660565
14-15	0.09504865812725828
16-17	0.09708680988730607
18-19	0.08889758934244342
20-21	0.08799445622721266
22-23	0.07059083700668489
24-25	0.08218511348599863
26-27	0.09380379896842671
28-29	0.08773816169451205
30-31	0.08272211155451421
32-33	0.09166801119592155
34-35	0.08526064787840605
36-37	0.08524844337684889
38-39	0.0756190916482399
40-41	0.08665196105592371
42-43	0.076546633766585
44-45	0.07322700934303412
46-47	0.06384174764556859
48-49	0.06618501194454568
50-51	0.06819875470147911
52-53	0.07984184918702154
54-55	0.08425987875071793
56-57	0.09174123820526459
58-59	0.06329254507549582
60-61	0.07921941960760574
62-63	0.08466262730210461
64-65	0.07600963569806941
66-67	0.07071288202225662
68-69	0.07803558295656003
70-71	0.07655883826814217
72-73	0.05960678560522978
74-75	0.08571221443602144
76-77	0.08910506586891535
78-79	0.07266560227140419
80-81	0.08385713019933123
82-83	0.08777477519918357
84-85	0.09122864913986335
86-87	0.07996389420259326
88-89	0.0603878737048888
90-91	0.08352760865728759
92-93	0.061278802318562385
94-95	0.08650550703723764
96-97	0.076192703221427
98-99	0.070456587489556
100-101	0.08537048839242062
102-103	0.05997292065194495
104-105	0.06653894248970367
106-107	0.08253904403115662
108-109	0.08860468130507128
110-111	0.08101348133651008
112-113	0.07195774118108819
114-115	0.08507758035504848
116-117	0.07874344404687603
118-119	0.07183569616551648
120-121	0.10306701565032052
122-123	0.08284415657008594
124-125	0.10292056163163446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4096849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.54083596678958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.78879899218593	30.416177393220213
2	14.316482142907489	13.039691297832443
3	6.739865120475564	9.208172756695921
4	3.6604498830347665	6.6679979075176155
5	2.2795856284732414	5.190711758927541
6	1.4890841144680456	4.068848123864482
7	1.0168330314929834	3.2415198404984733
8	0.7227338272259833	2.633112213867884
9	0.5251618008305116	2.152467668488153
>10	2.3402825337349933	17.619840368140053
>50	0.08792761256542672	2.7047271957212273
>100	0.03128649100122995	2.355443740783744
>500	0.0010777297467555358	0.3461171847009599
>1k	4.310918570426633E-4	0.35517254974133095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.322700934303413E-5
2	0.0	0.0	0.0	0.0	7.322700934303413E-5
3	0.0	0.0	0.0	0.0	7.322700934303413E-5
4	0.0	0.0	0.0	0.0	7.322700934303413E-5
5	0.0	0.0	0.0	0.0	7.322700934303413E-5
6	0.0	0.0	0.0	0.0	7.322700934303413E-5
7	0.0	0.0	0.0	0.0	7.322700934303413E-5
8	0.0	0.0	0.0	0.0	7.322700934303413E-5
9	0.0	0.0	0.0	0.0	7.322700934303413E-5
10-11	0.0	0.0	0.0	2.440900311434471E-5	1.0984051401455118E-4
12-13	0.0	0.0	0.0	2.440900311434471E-5	2.807035358149641E-4
14-15	0.0	0.0	0.0	2.440900311434471E-5	3.417260436008259E-4
16-17	0.0	0.0	0.0	3.6613504671517064E-5	4.027485513866877E-4
18-19	0.0	0.0	0.0	7.322700934303413E-5	4.3936205605820474E-4
20-21	0.0	0.0	0.0	8.543151090020649E-5	5.492025700727559E-4
22-23	0.0	0.0	0.0	1.9527202491475767E-4	6.468385825301348E-4
24-25	0.0	0.0	0.0	7.20065591873169E-4	6.834520872016518E-4
26-27	0.0	0.0	0.0	0.0018794932398045424	7.810880996590307E-4
28-29	0.0	0.0	0.0	0.005113686152455216	8.299061058877201E-4
30-31	0.0	0.0	0.0	0.017562277740771015	8.421106074448924E-4
32-33	0.0	0.0	0.0	0.0465723779421697	8.543151090020648E-4
34-35	0.0	0.0	0.0	0.09034992502774694	8.787241121164095E-4
36-37	0.0	0.0	0.0	0.1651879285763278	8.787241121164095E-4
38-39	0.0	0.0	0.0	0.28144801040995165	9.153376167879265E-4
40-41	0.0	0.0	0.0	0.4146113269002592	9.275421183450989E-4
42-43	0.0	0.0	0.0	0.5440766794187435	9.519511214594436E-4
44-45	0.0	0.0	0.0	0.7026619726526411	0.001000769127688133
46-47	0.0	0.0	0.0	0.8983855641250141	0.0010617916354739947
48-49	0.0	0.0	0.0	1.107900242356992	0.0010984051401455117
50-51	0.0	0.0	0.0	1.331669778407747	0.0011472231463742013
52-53	0.0	0.0	0.0	1.5553294739444876	0.001171632149488546
54-55	0.0	0.0	0.0	1.8079870651810697	0.0011960411526028907
56-57	0.0	0.0	0.0	2.1114153828954887	0.0012326546572744078
58-59	0.0	0.0	0.0	2.449846211075878	0.0012448591588315801
60-61	0.0	0.0	0.0	2.8036425067167476	0.0012570636603887525
62-63	0.0	0.0	0.0	3.158512798494648	0.001342495171288959
64-65	0.0	0.0	0.0	3.5457250193990553	0.0013546996728461312
66-67	0.0	0.0	0.0	3.9989635937277646	0.001379108675960476
68-69	0.0	0.0	0.0	4.50690274403572	0.0013913131775176483
70-71	0.0	0.0	0.0	5.057606467800009	0.0014279266821891653
72-73	2.440900311434471E-5	0.0	0.0	5.6153765979659	0.0014767446884178547
74-75	2.440900311434471E-5	0.0	0.0	6.190489324844533	0.0015011536915321994
76-77	2.440900311434471E-5	0.0	0.0	6.836571228278123	0.0015133581930893718
78-79	2.440900311434471E-5	0.0	0.0	7.5515231339988365	0.0015499716977608888
80-81	2.440900311434471E-5	0.0	0.0	8.30705500739715	0.0015743807008752337
82-83	2.440900311434471E-5	0.0	0.0	9.081528267212192	0.0016598122117754402
84-85	2.440900311434471E-5	0.0	0.0	9.859614059488159	0.0016598122117754402
86-87	2.440900311434471E-5	0.0	0.0	10.689727641902351	0.0017086302180041296
88-89	2.440900311434471E-5	0.0	0.0	11.58567230571593	0.0017696527257899913
90-91	2.440900311434471E-5	0.0	0.0	12.53595141046204	0.0017818572273471636
92-93	2.440900311434471E-5	0.0	0.0	13.493821715176711	0.0019283112460332318
94-95	2.440900311434471E-5	0.0	0.0	14.452399880981702	0.0019283112460332318
96-97	2.440900311434471E-5	0.0	0.0	15.429931637704978	0.0020259472584906106
98-99	2.440900311434471E-5	0.0	0.0	16.4658131163731	0.0020503562616049553
100-101	2.440900311434471E-5	0.0	0.0	17.574872786378016	0.0020503562616049553
102-103	2.440900311434471E-5	0.0	0.0	18.68725208080649	0.002086969766276472
104-105	2.440900311434471E-5	0.0	0.0	19.781104941871178	0.0021235832709479894
106-107	2.440900311434471E-5	1.2204501557172355E-5	0.0	20.865645768247745	0.0021235832709479894
108-109	2.440900311434471E-5	2.440900311434471E-5	0.0	21.983517088376946	0.002147992274062334
110-111	2.440900311434471E-5	2.440900311434471E-5	0.0	23.14825369448569	0.002147992274062334
112-113	2.440900311434471E-5	2.440900311434471E-5	0.0	24.331699801481577	0.002147992274062334
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9305	0.0	31.989891	1
GTACATG	15295	0.0	27.84693	1
TCAACGC	10350	0.0	27.661905	4
AACGCAG	10450	0.0	27.280281	6
GGTATCA	6875	0.0	27.158152	1
ATCAACG	10790	0.0	26.86488	3
CAACGCA	10715	0.0	26.66407	5
TACATGG	15440	0.0	26.637424	2
CATGGGG	9325	0.0	25.97903	4
ACATGGG	15710	0.0	25.650148	3
TATCAAC	11550	0.0	25.508486	2
ACGCAGA	11790	0.0	24.026817	7
CGCAGAG	12390	0.0	22.86357	8
GTATAGG	1075	0.0	20.531557	1
TACACCG	555	0.0	19.304428	5
GCAGAGT	14115	0.0	19.268312	9
AGAGTAC	12160	0.0	17.639538	10-11
GTACCGT	735	0.0	17.004412	6
ATGGGGA	5640	0.0	16.88567	5
CAGAGTA	13235	0.0	16.543957	10-11
>>END_MODULE