FastQCFastQC Report
Mon 15 Aug 2016
SRR2921103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096849
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84750.20686630139407142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74420.18165180117695331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.11540576672462177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87650.043.563271
GTATCAA132700.042.172021
TCAACGC163500.033.938194
CAACGCA165600.033.5438045
ATCAACG165900.033.3394433
TATCAAC172050.032.4248472
AACGCAG172150.032.296086
ACGCAGA192700.028.7105037
CGCAGAG199300.027.6403318
GTACATG151700.025.2881971
CATGGGG92000.025.1362574
GCAGAGT221150.024.4518229
TACATGG153800.024.220352
GAGTACT123500.024.08536512-13
ACATGGG155200.023.771453
AGAGTAC198700.022.55885310-11
GTACTTT132600.022.38908814-15
AGTACTT135100.022.28154812-13
GTACCGT5950.022.0097836
CAGAGTA211400.021.47094710-11
Produced by FastQC (version 0.11.5)