##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4096849
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.382848135237595	33.0	31.0	34.0	28.0	34.0
2	31.443087114023484	33.0	31.0	34.0	28.0	34.0
3	31.52590295614996	34.0	31.0	34.0	28.0	34.0
4	35.018252808438874	37.0	35.0	37.0	32.0	37.0
5	34.7935198490352	37.0	35.0	37.0	32.0	37.0
6	34.56491147220706	37.0	35.0	37.0	31.0	37.0
7	34.516080285116686	37.0	35.0	37.0	31.0	37.0
8	34.50671845606221	37.0	35.0	37.0	31.0	37.0
9	36.06910603734724	39.0	37.0	39.0	31.0	39.0
10-11	36.03454362120742	39.0	37.0	39.0	30.5	39.0
12-13	35.98563859688263	39.0	37.0	39.0	30.0	39.0
14-15	37.25064995073043	40.0	37.0	41.0	31.0	41.0
16-17	37.24743919046077	40.0	37.0	41.0	31.0	41.0
18-19	37.26988046178904	40.0	37.5	41.0	31.0	41.0
20-21	37.281427506847336	40.0	38.0	41.0	31.0	41.0
22-23	37.23723903419433	40.0	37.5	41.0	31.0	41.0
24-25	37.20108173379102	40.0	37.0	41.0	31.0	41.0
26-27	37.09023239567775	40.0	37.0	41.0	30.0	41.0
28-29	36.971262182228344	40.0	37.0	41.0	30.0	41.0
30-31	36.846380474359684	40.0	37.0	41.0	30.0	41.0
32-33	36.729336131256	40.0	36.5	41.0	30.0	41.0
34-35	36.59238099817689	40.0	36.0	41.0	30.0	41.0
36-37	36.42565212923395	40.0	36.0	41.0	29.5	41.0
38-39	36.289831282529576	40.0	36.0	41.0	29.0	41.0
40-41	36.16241799490291	40.0	35.5	41.0	28.5	41.0
42-43	36.011678609585076	40.0	35.0	41.0	28.0	41.0
44-45	35.84671878314285	40.0	35.0	41.0	27.0	41.0
46-47	35.59037299153569	40.0	35.0	41.0	26.5	41.0
48-49	35.34288717987897	40.0	35.0	41.0	25.5	41.0
50-51	35.15595412474319	39.0	35.0	41.0	24.5	41.0
52-53	34.95904645252973	39.0	35.0	41.0	24.0	41.0
54-55	34.74485037159046	39.0	34.5	41.0	23.5	41.0
56-57	34.509198532823646	38.5	34.0	41.0	23.0	41.0
58-59	34.264281890789725	38.0	34.0	40.5	22.5	41.0
60-61	33.98264349015548	38.0	34.0	40.0	22.0	41.0
62-63	33.69182998934059	37.0	34.0	40.0	22.0	41.0
64-65	33.37031679712872	37.0	33.0	40.0	20.5	41.0
66-67	33.028019948990064	36.0	33.0	39.0	20.0	41.0
68-69	32.67479018631148	35.5	33.0	39.0	18.5	41.0
70-71	32.28296917948404	35.0	32.5	38.5	17.5	40.0
72-73	31.902450639503677	35.0	32.0	37.5	15.5	40.0
74-75	31.577811874443015	35.0	32.0	37.0	13.0	39.0
76-77	31.26797265410563	35.0	32.0	36.5	12.0	39.0
78-79	30.96837410897985	35.0	31.5	36.0	10.5	38.5
80-81	30.931439259782334	35.0	32.0	36.0	10.5	37.0
82-83	30.73833011663354	35.0	32.0	35.0	7.5	37.0
84-85	30.542035232443276	35.0	32.0	35.0	7.0	36.5
86-87	30.37490337085892	35.0	32.0	35.0	6.5	36.0
88-89	30.199208220756976	35.0	31.0	35.0	2.0	36.0
90-91	30.051550716172358	35.0	31.0	35.0	2.0	36.0
92-93	29.956467153170642	35.0	31.0	35.0	2.0	35.0
94-95	29.85600640882786	35.0	31.0	35.0	2.0	35.0
96-97	29.793033743738178	35.0	31.0	35.0	2.0	35.0
98-99	29.70342231309965	35.0	31.0	35.0	2.0	35.0
100-101	29.6153209454388	35.0	31.0	35.0	2.0	35.0
102-103	28.299747195954744	33.0	28.0	34.5	2.0	35.0
104-105	29.31493228088221	34.0	30.5	35.0	2.0	35.0
106-107	29.48061375950151	35.0	31.0	35.0	2.0	35.0
108-109	29.480441553984537	35.0	31.0	35.0	2.0	35.0
110-111	29.395723274155333	35.0	31.0	35.0	2.0	35.0
112-113	29.285362970419463	35.0	31.0	35.0	2.0	35.0
114-115	29.20122391623416	35.0	31.0	35.0	2.0	35.0
116-117	29.090489422480545	34.5	31.0	35.0	2.0	35.0
118-119	28.940183419013003	34.0	30.0	35.0	2.0	35.0
120-121	29.06069017920846	35.0	31.0	35.0	2.0	35.0
122-123	28.97107862652492	35.0	31.0	35.0	2.0	35.0
124-125	28.289003817323994	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64387.0
3	25553.0
4	11289.0
5	9793.0
6	11514.0
7	13960.0
8	15167.0
9	17138.0
10	19355.0
11	22090.0
12	24280.0
13	28332.0
14	35491.0
15	32519.0
16	19326.0
17	16599.0
18	15392.0
19	14875.0
20	15057.0
21	15958.0
22	17208.0
23	18977.0
24	24233.0
25	31526.0
26	38929.0
27	47134.0
28	56612.0
29	67610.0
30	81258.0
31	99622.0
32	125337.0
33	163006.0
34	224504.0
35	341478.0
36	664232.0
37	1212545.0
38	453747.0
39	816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82235304471978	18.2615466609296	12.418026775149375	25.49807351920124
2	16.61914549777611	20.465162263635076	37.278282012098366	25.63741022649045
3	19.23261023288874	23.951627213988118	29.625109443867714	27.19065310925543
4	13.402810510819336	16.38467905698572	35.70379816110214	34.508712271092804
5	13.834625352049335	37.238271193657134	34.480145065223375	14.446958389070149
6	33.4371067277403	36.01939100935646	16.436288916854917	14.107213346048322
7	28.54167561719595	31.871467795670156	21.00045598878287	18.58640059835102
8	26.79874612558322	34.44387945287877	19.64186803950666	19.11550638203135
9	26.604059383341855	14.683279485699149	19.080444337576456	39.63221679338254
10-11	25.452312598357345	25.317092984568685	27.77801916630316	21.45257525077081
12-13	26.281521436114456	22.94746634808561	27.626047907261274	23.14496430853866
14-15	23.469024820422685	24.276057782090483	25.167725516761568	27.087191880725264
16-17	23.29186648191385	27.12354660956125	26.014614709331678	23.56997219919322
18-19	23.241254136410706	26.068191558615545	27.943331859978294	22.747222444995458
20-21	23.999816405039777	25.307832839718426	27.811169653706212	22.88118110153558
22-23	23.683421863492608	25.189193465081377	27.583774254746878	23.54361041667913
24-25	23.374945081452797	25.410926236471482	27.772445129367284	23.44168355270844
26-27	23.283608107078752	25.496194313638554	27.82277044592058	23.397427133362108
28-29	23.186587129022758	25.595470319135277	27.848421120476875	23.36952143136509
30-31	23.497233738900256	25.491351474283313	27.55356216619589	23.457852620620546
32-33	23.263373385434026	25.531914114428645	27.682959729388916	23.521752770748417
34-35	23.504886683771463	25.58235290814509	27.718719108030974	23.19404130005247
36-37	23.636293500110536	25.407078525758596	27.5179700011487	23.43865797298216
38-39	23.436339788860263	25.593878571153923	27.545111480974903	23.424670159010912
40-41	23.525459407811848	25.497116772161828	27.592973217848776	23.384450602177548
42-43	23.675218046827286	25.349487111283747	27.41504355565408	23.560251286234884
44-45	23.57051871641694	25.574801819207742	27.226440676145224	23.628238788230085
46-47	23.620084815314463	25.525847503094905	27.074174397171962	23.77989328441867
48-49	23.63090128021919	25.682640548058178	26.85555125204019	23.830906919682445
50-51	23.478300990676203	25.91067094077499	26.75328644703882	23.857741621509984
52-53	23.65084941229087	25.826086043702475	26.647472660320037	23.875591883686617
54-55	23.502870429854667	25.883537119551015	26.588696959972424	24.024895490621894
56-57	23.4884802734761	25.894383455116017	26.457367192022947	24.159769079384944
58-59	23.3156194687403	25.65857578770997	26.765349537469053	24.260455206080678
60-61	23.364347489401137	25.762891113067397	26.77531874711012	24.097442650421343
62-63	23.209123587271243	25.793930034383607	26.784315587531964	24.212630790813186
64-65	23.136618912783803	25.71385147445785	26.793755352245668	24.355774260512682
66-67	23.112076693664786	25.798273865209925	26.665052118020903	24.424597323104386
68-69	23.27091945585014	25.561118230260554	26.724063639255487	24.44389867463382
70-71	23.031132842584746	25.51222865379763	26.785346099583933	24.67129240403369
72-73	23.131497165760987	25.464128613117587	26.707782828144715	24.696591392976714
74-75	23.042844641743525	25.51563257369959	26.644286610062263	24.79723617449462
76-77	22.9463548810665	25.37848468448808	26.806240789467584	24.868919644977836
78-79	22.954506894577715	25.382762884383236	26.665958716755345	24.996771504283707
80-81	22.807760408897927	25.39825778468703	26.689026797246967	25.10495500916808
82-83	22.68989785718706	25.377632773389575	26.734719560420082	25.197749809003277
84-85	22.689771585305223	25.326346376772502	26.57798825500919	25.40589378291309
86-87	22.621234749689613	25.29858605229766	26.635259982121212	25.44491921589152
88-89	22.62950089262554	25.21310404809497	26.568271358164093	25.5891237011154
90-91	22.456096749985228	25.223440651575157	26.56996811164391	25.7504944867957
92-93	22.436724798371593	25.115293829293115	26.62300583969867	25.824975532636618
94-95	22.213039438692288	25.02530249480402	26.716920131021272	26.04473793548242
96-97	22.313496347488798	24.907204096788984	26.653737250633895	26.125562305088323
98-99	22.23478305344896	24.939438849442507	26.498947221682066	26.32683087542646
100-101	22.123915087441222	24.91670200742852	26.58072086364765	26.37866204148261
102-103	21.978835534486247	24.766943571597547	26.650913512841818	26.603307381074394
104-105	21.807033516598402	24.89201650203407	26.519504528856043	26.781445452511488
106-107	21.673135801540823	24.99314594773538	26.523693048806958	26.810025201916837
108-109	21.708113470634416	24.920358765149533	26.51988431088423	26.851643453331825
110-111	21.670343444227484	25.04423851885923	26.374335994968725	26.91108204194456
112-113	21.448224653987356	25.03448621851244	26.440081133704	27.077207993796204
114-115	21.379699672575363	25.116087748581823	26.3520989658508	27.15211361299201
116-117	21.272017298931413	25.10802067552347	26.403731729858965	27.216230295686156
118-119	21.254220589642493	25.11627980776178	26.291502871660878	27.33799673093485
120-121	21.15237734671864	25.226113210752672	26.313260499652042	27.308248942876652
122-123	21.057417915342562	25.24465479571732	26.27130114817279	27.42662614076733
124-125	20.901429215655284	25.270358588444353	26.258342583377015	27.56986961252335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	107.5
2	250.5
3	506.0
4	400.0
5	241.0
6	422.5
7	899.0
8	1457.5
9	1642.5
10	1353.0
11	1083.5
12	1104.5
13	1252.5
14	1430.0
15	1615.5
16	1843.0
17	2108.5
18	2473.5
19	2821.5
20	3174.5
21	3814.5
22	4761.0
23	5768.5
24	7041.0
25	8877.5
26	11800.5
27	15308.5
28	18876.0
29	23443.0
30	28880.0
31	34813.0
32	42389.0
33	49910.5
34	56341.5
35	62565.5
36	70480.0
37	79007.5
38	85975.0
39	93757.5
40	100902.5
41	108861.5
42	119027.5
43	128745.0
44	138143.0
45	148172.0
46	156777.5
47	163358.0
48	169047.5
49	174072.0
50	178914.5
51	179618.0
52	175952.0
53	172691.0
54	167506.5
55	157543.5
56	146678.0
57	134362.5
58	122150.5
59	108814.0
60	91738.0
61	76104.0
62	62240.0
63	47629.0
64	34863.0
65	26285.5
66	19846.0
67	14618.0
68	10704.5
69	7892.0
70	5910.0
71	4528.5
72	3522.5
73	2908.5
74	2162.5
75	1560.0
76	1086.5
77	623.0
78	383.0
79	228.5
80	145.0
81	101.0
82	78.0
83	54.5
84	41.0
85	33.5
86	26.0
87	19.5
88	17.5
89	14.0
90	9.0
91	7.0
92	10.5
93	7.5
94	8.5
95	15.5
96	16.0
97	11.0
98	6.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1583900212089828
2	0.01047146233605388
3	0.0
4	0.0038078044858377747
5	0.13298024896695
6	0.07305614632123371
7	0.00627311380038659
8	0.010398235326710846
9	0.010105327289338708
10-11	0.004393620560582048
12-13	0.008567560093134993
14-15	0.010349417320482156
16-17	0.013791086759604759
18-19	0.018441001852887426
20-21	0.021382286728165965
22-23	0.02381098253804326
24-25	0.024665297647045326
26-27	0.02130905971882293
28-29	0.020576789625392587
30-31	0.02385980054427195
32-33	0.022798008908797955
34-35	0.02250510087142582
36-37	0.022590532382326025
38-39	0.018221320824858327
40-41	0.0245432526314736
42-43	0.021540945248409202
44-45	0.029778983799500544
46-47	0.024616479640816636
48-49	0.017440232725199293
50-51	0.013571405731575658
52-53	0.014767446884178546
54-55	0.016732371634883298
56-57	0.013412747211332416
58-59	0.012900158145931177
60-61	0.015987897039895782
62-63	0.013803291261161931
64-65	0.02493379668130312
66-67	0.01857525137001632
68-69	0.017037484173812605
70-71	0.024347980606558847
72-73	0.02970575679015751
74-75	0.015682784500966475
76-77	0.007713244984132927
78-79	0.01254622760077318
80-81	0.013913131775176484
82-83	0.014022972289191036
84-85	0.013412747211332416
86-87	0.009153376167879266
88-89	0.019197680949432114
90-91	0.016671349127097435
92-93	0.01405958579386255
94-95	0.010129736292453053
96-97	0.01862406937624501
98-99	0.019100044936974734
100-101	0.024701911151716844
102-103	0.018013844298386393
104-105	0.01233875107430125
106-107	0.017867390279700326
108-109	0.008616378099363681
110-111	0.02126024171259424
112-113	0.015804829516538197
114-115	0.011874980015128699
116-117	0.010032100279995675
118-119	0.006468385825301347
120-121	0.002880262367492676
122-123	0.005113686152455216
124-125	5.247935669584112E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4096849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.21915613554521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.66442325436392	27.947418050453354
2	15.343177281934944	13.26238349126593
3	6.891768966087697	8.935693169863475
4	3.955881012094519	6.838793564614065
5	2.4168031218896253	5.222609573691041
6	1.599050761920645	4.146577472886654
7	1.0472192348744176	3.1681952134128553
8	0.79642160901538	2.753653589580628
9	0.5748480153892055	2.2360001518182937
>10	2.5716584549343944	18.4232451341652
>50	0.09832177489066107	2.8635244023702615
>100	0.037938540260090706	2.7656489847328642
>500	0.0018094344751779085	0.5447518438519688
>1k	5.654482245002768E-4	0.5025226058668725
>5k	1.1308964490004907E-4	0.38898275142648364
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8475	0.20686630139407142	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7442	0.18165180117695331	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4728	0.11540576672462177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.763601245737884E-5
2	0.0	0.0	0.0	0.0	9.763601245737884E-5
3	0.0	0.0	0.0	0.0	9.763601245737884E-5
4	0.0	0.0	0.0	2.440900311434471E-5	9.763601245737884E-5
5	0.0	0.0	0.0	2.440900311434471E-5	9.763601245737884E-5
6	0.0	0.0	0.0	2.440900311434471E-5	9.763601245737884E-5
7	0.0	0.0	0.0	2.440900311434471E-5	9.763601245737884E-5
8	0.0	0.0	0.0	2.440900311434471E-5	9.763601245737884E-5
9	0.0	0.0	0.0	4.881800622868942E-5	9.763601245737884E-5
10-11	0.0	0.0	0.0	4.881800622868942E-5	9.763601245737884E-5
12-13	0.0	0.0	0.0	4.881800622868942E-5	2.3188552958627472E-4
14-15	0.0	0.0	0.0	6.102250778586177E-5	2.807035358149641E-4
16-17	0.0	0.0	0.0	8.543151090020649E-5	3.5393054515799825E-4
18-19	0.0	0.0	0.0	9.763601245737884E-5	4.3936205605820474E-4
20-21	0.0	0.0	0.0	1.0984051401455118E-4	4.881800622868941E-4
22-23	0.0	0.0	0.0	2.4409003114344706E-4	5.125890654012388E-4
24-25	0.0	0.0	0.0	7.56679096544686E-4	5.125890654012388E-4
26-27	0.0	0.0	0.0	0.0019039022429188871	5.369980685155835E-4
28-29	0.0	0.0	0.0	0.005223526666469767	5.614070716299283E-4
30-31	0.0	0.0	0.0	0.017794163270357294	5.736115731871007E-4
32-33	0.0	0.0	0.0	0.04674324096397012	5.980205763014454E-4
34-35	0.0	0.0	0.0	0.09047197004331865	6.590430840873071E-4
36-37	0.0	0.0	0.0	0.1655906771277145	6.590430840873071E-4
38-39	0.0	0.0	0.0	0.2820948489924818	6.834520872016518E-4
40-41	0.0	0.0	0.0	0.41534359699368956	6.834520872016518E-4
42-43	0.0	0.0	0.0	0.5450042215370887	6.834520872016518E-4
44-45	0.0	0.0	0.0	0.7041509218426161	6.834520872016518E-4
46-47	0.0	0.0	0.0	0.8999843538290038	7.078610903159965E-4
48-49	0.0	0.0	0.0	1.1106828687120274	7.566790965446859E-4
50-51	0.0	0.0	0.0	1.3349649938281836	8.543151090020648E-4
52-53	0.0	0.0	0.0	1.5600892295517848	8.787241121164095E-4
54-55	0.0	0.0	0.0	1.8143700194954708	8.787241121164095E-4
56-57	0.0	0.0	0.0	2.119433740418551	9.031331152307542E-4
58-59	0.0	0.0	0.0	2.460122401387017	9.275421183450989E-4
60-61	0.0	0.0	0.0	2.8174580024794666	9.519511214594436E-4
62-63	0.0	0.0	0.0	3.176075076235419	0.0010251781308024776
64-65	0.0	0.0	0.0	3.5683155517813816	0.0010495871339168223
66-67	0.0	0.0	0.0	4.024446592979141	0.001073996137031167
68-69	0.0	0.0	0.0	4.535949457741792	0.001073996137031167
70-71	0.0	0.0	0.0	5.090668462518389	0.0011228141432598564
72-73	0.0	0.0	0.0	5.651916875628074	0.0012326546572744078
74-75	0.0	0.0	0.0	6.229714592849285	0.0013180861681746142
76-77	0.0	0.0	0.0	6.878115351578737	0.0013546996728461312
78-79	0.0	0.0	0.0	7.597131356317989	0.0013669041744033036
80-81	0.0	0.0	0.0	8.357300940308026	0.0013913131775176483
82-83	0.0	0.0	0.0	9.136802454764625	0.0014645401868606824
84-85	0.0	0.0	0.0	9.921527495887693	0.0014645401868606824
86-87	0.0	0.0	0.0	10.757718920077357	0.0014767446884178547
88-89	0.0	0.0	0.0	11.66042487775361	0.0015011536915321994
90-91	0.0	0.0	0.0	12.618344000474512	0.0015133581930893718
92-93	0.0	0.0	0.0	13.583402756606358	0.0016231987071039231
94-95	0.0	0.0	0.0	14.550865799544967	0.0016354032086610955
96-97	0.0	0.0	0.0	15.537343455909651	0.001830675233575853
98-99	0.0	0.0	0.0	16.581523995636648	0.001977129252261921
100-101	0.0	1.2204501557172355E-5	0.0	17.70222676012711	0.0019893337538190933
102-103	0.0	2.440900311434471E-5	0.0	18.824320837795096	0.0020259472584906106
104-105	0.0	2.440900311434471E-5	0.0	19.92711959850119	0.0020503562616049553
106-107	0.0	2.440900311434471E-5	0.0	21.021277572104808	0.0020747652647193
108-109	0.0	2.440900311434471E-5	0.0	22.151023872249137	0.0020747652647193
110-111	0.0	2.440900311434471E-5	0.0	23.327305936830964	0.0020747652647193
112-113	0.0	2.440900311434471E-5	0.0	24.5241892000413	0.0020747652647193
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8765	0.0	43.56327	1
GTATCAA	13270	0.0	42.17202	1
TCAACGC	16350	0.0	33.93819	4
CAACGCA	16560	0.0	33.543804	5
ATCAACG	16590	0.0	33.339443	3
TATCAAC	17205	0.0	32.424847	2
AACGCAG	17215	0.0	32.29608	6
ACGCAGA	19270	0.0	28.710503	7
CGCAGAG	19930	0.0	27.640331	8
GTACATG	15170	0.0	25.288197	1
CATGGGG	9200	0.0	25.136257	4
GCAGAGT	22115	0.0	24.451822	9
TACATGG	15380	0.0	24.22035	2
GAGTACT	12350	0.0	24.085365	12-13
ACATGGG	15520	0.0	23.77145	3
AGAGTAC	19870	0.0	22.558853	10-11
GTACTTT	13260	0.0	22.389088	14-15
AGTACTT	13510	0.0	22.281548	12-13
GTACCGT	595	0.0	22.009783	6
CAGAGTA	21140	0.0	21.470947	10-11
>>END_MODULE