FastQCFastQC Report
Mon 15 Aug 2016
SRR2921102_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921102_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468314
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG141500.030.200962
GTACATG144900.030.047691
GTATCAA84300.028.6539211
CATGGGG87850.027.8465634
ACATGGG150900.027.7292183
TCAACGC93300.025.0078264
AACGCAG93500.024.8241486
CAACGCA95200.024.3833265
ATCAACG97550.023.91833
TATCAAC101750.023.3394812
GGTATCA64850.021.9808521
ACGCAGA104100.021.951827
CGCAGAG107250.021.1954968
GTATAGC12450.021.078511
TACCGTA3251.454282E-820.141857
GTGTAGC25550.018.6747881
ATGGGGA46350.018.3633375
GCAGAGT119200.018.3217669
ACCGTCG3604.7022695E-818.183098
ATGGGGG46700.017.0786365
Produced by FastQC (version 0.11.5)