##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921102_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3468314
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.472031367402145	31.0	30.0	34.0	23.0	34.0
2	29.49869302491066	31.0	30.0	34.0	23.0	34.0
3	29.689906969207517	31.0	30.0	34.0	23.0	34.0
4	33.052775786736724	37.0	35.0	37.0	26.0	37.0
5	33.10909335198601	37.0	35.0	37.0	26.0	37.0
6	33.0844046992285	37.0	35.0	37.0	26.0	37.0
7	33.020808957897124	37.0	35.0	37.0	26.0	37.0
8	32.94750071648645	37.0	35.0	37.0	25.0	37.0
9	34.371806301274916	39.0	35.0	39.0	25.0	39.0
10-11	34.32984859502341	39.0	35.0	39.0	25.0	39.0
12-13	34.24243076030602	39.0	35.0	39.0	25.0	39.0
14-15	35.38973936615889	40.0	36.0	41.0	25.0	41.0
16-17	35.3414956085291	40.0	36.0	41.0	25.0	41.0
18-19	35.35596603998369	40.0	36.0	41.0	25.0	41.0
20-21	35.39756579133262	40.0	36.0	41.0	25.0	41.0
22-23	35.3118572885846	40.0	35.0	41.0	24.0	41.0
24-25	35.28281522376578	40.0	35.0	41.0	24.0	41.0
26-27	35.208568053526875	40.0	35.0	41.0	24.0	41.0
28-29	35.08067349150048	40.0	35.0	41.0	24.0	41.0
30-31	34.981258040650296	40.0	35.0	41.0	23.5	41.0
32-33	34.86371389672331	40.0	35.0	41.0	23.0	41.0
34-35	34.749965401056535	40.0	34.5	41.0	22.0	41.0
36-37	34.62720892629675	39.5	34.0	41.0	20.5	41.0
38-39	34.492205578848974	39.0	34.0	41.0	19.0	41.0
40-41	34.363227493243116	39.0	34.0	41.0	18.0	41.0
42-43	34.170726612411684	39.0	33.5	41.0	16.0	41.0
44-45	33.980289414395585	39.0	33.0	41.0	15.0	41.0
46-47	33.808471926128945	39.0	33.0	41.0	14.0	41.0
48-49	33.58619649201312	38.0	33.0	40.5	10.5	41.0
50-51	33.41466372421874	38.0	33.0	40.0	8.5	41.0
52-53	33.23520116690703	38.0	33.0	40.0	8.0	41.0
54-55	33.00905512015348	38.0	32.0	40.0	7.0	41.0
56-57	32.76370262323423	38.0	32.0	40.0	2.0	41.0
58-59	32.48606008567852	37.0	31.5	40.0	2.0	41.0
60-61	32.191113319036276	37.0	31.0	40.0	2.0	41.0
62-63	31.90773283503166	36.0	31.0	40.0	2.0	41.0
64-65	31.593226276513604	36.0	31.0	39.0	2.0	41.0
66-67	31.250788711748704	35.0	30.5	39.0	2.0	40.5
68-69	30.885974856947783	35.0	30.0	38.5	2.0	40.0
70-71	30.52758054778201	35.0	30.0	38.0	2.0	40.0
72-73	30.162981062268294	35.0	29.0	37.0	2.0	39.5
74-75	30.115897522542653	35.0	30.0	37.0	2.0	39.0
76-77	30.15726243356282	35.0	30.0	36.5	2.0	39.0
78-79	29.971078310671984	35.0	30.0	36.0	2.0	39.0
80-81	29.7450612891451	35.0	30.0	36.0	2.0	37.0
82-83	29.492563822076086	35.0	30.0	35.0	2.0	37.0
84-85	29.231845213553328	35.0	30.0	35.0	2.0	36.5
86-87	29.071074879610094	35.0	30.0	35.0	2.0	36.0
88-89	28.890235860997592	34.5	29.5	35.0	2.0	36.0
90-91	28.738154186731652	34.0	29.0	35.0	2.0	36.0
92-93	28.623494297229144	34.0	29.0	35.0	2.0	35.0
94-95	28.507137329549746	34.0	29.0	35.0	2.0	35.0
96-97	28.409880852771693	34.0	29.0	35.0	2.0	35.0
98-99	28.308273703015356	34.0	29.0	35.0	2.0	35.0
100-101	28.179074472495856	34.0	29.0	35.0	2.0	35.0
102-103	26.883207806444283	32.5	26.0	34.5	2.0	35.0
104-105	27.843014502147152	34.0	28.5	35.0	2.0	35.0
106-107	27.99609925744901	34.0	29.0	35.0	2.0	35.0
108-109	27.940165452147642	34.0	29.0	35.0	2.0	35.0
110-111	27.848978927513485	34.0	29.0	35.0	2.0	35.0
112-113	27.742778047201032	34.0	29.0	35.0	2.0	35.0
114-115	27.6116825062552	34.0	28.0	35.0	2.0	35.0
116-117	27.478537410395944	34.0	27.0	35.0	2.0	35.0
118-119	27.279695408201217	34.0	27.0	35.0	2.0	35.0
120-121	27.075743286219183	34.0	27.0	35.0	2.0	35.0
122-123	26.782442564312227	34.0	26.0	35.0	2.0	35.0
124-125	25.523891579597464	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155557.0
3	22836.0
4	15164.0
5	14459.0
6	18886.0
7	19557.0
8	18305.0
9	19583.0
10	20689.0
11	21476.0
12	21668.0
13	21541.0
14	20714.0
15	18756.0
16	16016.0
17	14635.0
18	13869.0
19	13840.0
20	14071.0
21	15382.0
22	18296.0
23	23292.0
24	30807.0
25	38480.0
26	43811.0
27	48638.0
28	56460.0
29	66320.0
30	78114.0
31	94303.0
32	115753.0
33	147987.0
34	201680.0
35	300009.0
36	545838.0
37	875983.0
38	285201.0
39	338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55989981445086	18.191796570825154	11.691244145023845	25.557059469700143
2	16.169562904154677	20.985199701316493	37.86055819654252	24.98467919798631
3	19.044255325779552	24.596680064255334	29.118185892047695	27.240878717917422
4	12.910847775053998	16.491596123352085	36.17044669176263	34.42710940983129
5	13.835740533221205	38.11192837359827	34.01538372296716	14.036947370213365
6	32.850288963441265	36.24969074170892	17.002349641289253	13.897670653560562
7	28.249588736385146	32.557876387392284	21.133133371224336	18.059401504998235
8	25.89193395771645	35.571132739817216	19.986831493340983	18.550101809125355
9	26.455572035696406	15.901379347374874	18.875425723607446	38.76762289332127
10-11	24.949979833196362	26.32809044540857	27.7631154572958	20.958814264099264
12-13	25.347558598343607	24.051213724405475	27.446285873746806	23.154941803504112
14-15	23.021226954876163	25.388562455283537	24.64152510601356	26.948685483826747
16-17	23.260018999491894	27.888746040750345	25.514790843243514	23.336444116514247
18-19	23.270685826853004	27.31697364175715	26.626258043774758	22.786082487615083
20-21	24.330352343761387	26.59204597121979	26.165957384792378	22.911644300226442
22-23	23.903190209203245	26.490253392885716	25.843134137556877	23.76342226035416
24-25	23.431570703260945	26.581617134839732	26.22934025603452	23.757471905864808
26-27	23.473135687617276	26.63364217791347	26.125994562330522	23.767227572138733
28-29	23.45533988301535	26.854682279049236	26.175780567423306	23.514197270512103
30-31	23.718616137749056	26.765553271332255	25.959854743405796	23.555975847512897
32-33	23.384511798310314	26.849004167861363	26.03476830151172	23.731715732316605
34-35	23.617283795637164	26.84096543359192	26.093519290880657	23.448231479890264
36-37	23.684317606093504	26.749481559206718	25.98242582386346	23.583775010836323
38-39	23.50910508504312	26.840249242829966	26.041498342536336	23.609147329590574
40-41	23.600000981207426	26.79189759311262	26.09740474964998	23.510696676029983
42-43	23.455481277288655	26.6963872714361	26.14476365150511	23.703367799770135
44-45	23.518143084436886	26.74308016636571	26.112124879314454	23.62665186988295
46-47	23.370743757588066	26.76526442252208	26.055635828143824	23.808355991746026
48-49	23.346604993527666	26.772779358012112	26.137577571697335	23.74303807676289
50-51	23.191993219483166	26.8969541774115	26.122229635907885	23.788822967197447
52-53	23.315567277753573	26.69244043273379	26.004513174487293	23.98747911502535
54-55	23.22630905106631	26.65975126186418	26.126580188995735	23.987359498073783
56-57	23.293368050785528	26.62867930147963	26.053875929169006	24.02407671856583
58-59	23.238311902359293	26.386591353509008	26.247698156866505	24.12739858726519
60-61	23.261894359698296	26.425528267879272	26.25159361940473	24.060983753017705
62-63	23.126650892553354	26.354139521541647	26.331283506643683	24.187926079261313
64-65	23.054205742317126	26.260943586784013	26.323935939979805	24.36091473091906
66-67	23.076718877882772	26.353677332289582	26.22018331946648	24.349420470361167
68-69	23.151939971567696	26.249222858556	26.274703142428873	24.324134027447432
70-71	22.97990367580637	25.989641563827508	26.524761544886438	24.50569321547968
72-73	23.044951143072367	26.013785936579037	26.497190968284322	24.44407195206427
74-75	22.856233802963228	26.017794374830455	26.59689074611704	24.52908107608928
76-77	22.796907415688636	25.852507584058117	26.76584131277402	24.58474368747923
78-79	22.77643916038031	25.87654432131665	26.79709565951806	24.549920858784976
80-81	22.74655204531101	25.888096423690765	26.808763056400824	24.5565884745974
82-83	22.696296582754133	25.7578916822857	26.886213832299706	24.65959790266046
84-85	22.815346386063748	25.70447513939716	26.70814910589566	24.77202936864343
86-87	22.703152565541917	25.643039366777188	26.882232105226635	24.771575962454268
88-89	22.72046252020309	25.47352118576341	26.9053511572526	24.9006651367809
90-91	22.718956274109352	25.523973933979587	26.92291154250504	24.834158249406023
92-93	22.639720663258867	25.51721799993797	26.982076950319055	24.860984386484105
94-95	22.4563640878835	25.426987001252073	27.063940656804515	25.05270825405992
96-97	22.488788674779663	25.348719033412216	27.11775808700063	25.04473420480749
98-99	22.425459368288266	25.415221496429087	26.987395513849183	25.171923621433468
100-101	22.325311931384476	25.560293266726404	27.000148624644854	25.11424617724427
102-103	22.21381694636328	25.486248574396935	27.121223792777826	25.178710686461958
104-105	22.009519471955382	25.584955004459953	27.069485393671123	25.33604012991354
106-107	21.961262171855015	25.582737591507605	27.140316589074136	25.315683647563247
108-109	21.918777207886432	25.67322022471651	27.125317605717537	25.282684961679518
110-111	21.87792276976344	25.824623281039834	27.076019645341503	25.221434303855222
112-113	21.96373623329586	25.90480515972955	26.997557138603096	25.133901468371494
114-115	21.82280290718287	25.946617187205774	27.070250955119725	25.160328950491635
116-117	21.71904975171468	25.83575539452087	27.107885800295957	25.337309053468495
118-119	21.7913947815368	25.805469616097334	27.0846354099038	25.318500192462068
120-121	21.816772180854578	25.89505213362071	27.078438996173432	25.20973668935128
122-123	21.87514609403001	25.941422714887363	26.988422715464527	25.195008475618096
124-125	21.827892121560247	26.01609777006773	26.840036888919037	25.315973219452985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2132.0
1	1585.5
2	883.5
3	663.0
4	534.0
5	440.0
6	403.0
7	395.5
8	416.5
9	450.5
10	486.5
11	543.0
12	567.0
13	614.0
14	746.0
15	869.0
16	1005.5
17	1210.0
18	1484.5
19	1807.5
20	2169.5
21	2731.5
22	3497.5
23	4561.5
24	5867.5
25	7667.0
26	10297.0
27	13601.0
28	16727.0
29	20506.5
30	25420.5
31	30486.0
32	37016.5
33	43975.0
34	50486.0
35	57462.5
36	65786.5
37	73367.5
38	79382.5
39	85741.5
40	92616.0
41	98830.0
42	104159.5
43	111784.5
44	119922.0
45	126356.5
46	132596.0
47	139192.5
48	145025.5
49	149468.5
50	151883.5
51	149912.0
52	144756.0
53	141576.5
54	137408.0
55	129075.5
56	118801.0
57	107366.5
58	97134.5
59	85875.0
60	71976.0
61	58830.0
62	47939.0
63	37240.0
64	27461.0
65	20562.5
66	15418.0
67	11619.0
68	8962.5
69	6953.5
70	5690.0
71	4579.0
72	3425.0
73	2823.0
74	2277.0
75	1720.5
76	1233.0
77	820.5
78	549.5
79	371.5
80	270.5
81	187.0
82	136.0
83	109.0
84	83.5
85	61.5
86	41.5
87	28.0
88	31.5
89	32.0
90	22.0
91	19.0
92	15.0
93	12.0
94	16.5
95	16.0
96	12.0
97	16.0
98	20.0
99	20.0
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28633508961414683
2	0.07029352013687343
3	0.07972173223070345
4	0.08502690356178823
5	0.09292699565264276
6	0.1263149760950133
7	0.11507031946934446
8	0.11498382211068549
9	0.12144229155722347
10-11	0.09980353566603253
12-13	0.08854446281392053
14-15	0.09880881604145415
16-17	0.10046668208241814
18-19	0.09260983867089312
20-21	0.09196110848095068
22-23	0.07408498769142587
24-25	0.08649735865899108
26-27	0.09833308056882971
28-29	0.09106730244147446
30-31	0.08643969375321842
32-33	0.09651663603699089
34-35	0.0897986745144759
36-37	0.0898419231938054
38-39	0.08009655411822575
40-41	0.09213410319826867
42-43	0.08122101978079263
44-45	0.07773229298154666
46-47	0.06764093447133103
48-49	0.06941413032384035
50-51	0.0721243808951554
52-53	0.08372944318190337
54-55	0.08948151753272628
56-57	0.09774201528465992
58-59	0.06751118843334254
60-61	0.08453675186272061
62-63	0.09062039942173632
64-65	0.08113452242213363
66-67	0.07541128052419706
68-69	0.08331137261505159
70-71	0.08158142544187175
72-73	0.06265292012199587
74-75	0.09151420546121257
76-77	0.09559399754462831
78-79	0.07708356279160422
80-81	0.08959684734427159
82-83	0.09392171527722115
84-85	0.09785734509620524
86-87	0.08630994771522993
88-89	0.06516134352310661
90-91	0.08975542583514641
92-93	0.06477210540914116
94-95	0.09369105565413051
96-97	0.08202832846160989
98-99	0.0750652910895611
100-101	0.09252334131223412
102-103	0.06449819710672101
104-105	0.07123057485567916
106-107	0.08927969036252197
108-109	0.09589673829993478
110-111	0.08837146809660257
112-113	0.07802061751040996
114-115	0.09147095678188306
116-117	0.08494040620312925
118-119	0.07773229298154666
120-121	0.11103377606525823
122-123	0.08880395488989751
124-125	0.11126443568834886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3468314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.02947314511091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32401459839544	33.98919912063306
2	14.130242229705987	13.855966638705636
3	6.178048440737913	9.087193803430617
4	3.3049080158942514	6.481515952493959
5	1.8921208963727478	4.638484533800541
6	1.2836462807079074	3.776190050867392
7	0.8428768865873573	2.892806677289867
8	0.579868914415492	2.274453389361519
9	0.44397924834084934	1.959126177016278
>10	1.909514093098243	15.756272370940504
>50	0.08342324416552421	2.7374666145975466
>100	0.02635141379180984	2.059638045540928
>500	7.690936030056556E-4	0.2631456007565019
>1k	2.366441835097965E-4	0.22854102456560227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.883245288633036E-5	0.0
3	0.0	0.0	0.0	2.883245288633036E-5	0.0
4	0.0	0.0	0.0	2.883245288633036E-5	0.0
5	0.0	0.0	0.0	8.649735865899108E-5	2.883245288633036E-5
6	0.0	0.0	0.0	8.649735865899108E-5	8.649735865899108E-5
7	2.883245288633036E-5	0.0	0.0	8.649735865899108E-5	1.1532981154532144E-4
8	2.883245288633036E-5	0.0	0.0	8.649735865899108E-5	1.441622644316518E-4
9	2.883245288633036E-5	0.0	0.0	1.1532981154532144E-4	1.7299471731798216E-4
10-11	4.324867932949554E-5	0.0	0.0	2.0182717020431252E-4	1.7299471731798216E-4
12-13	5.766490577266072E-5	0.0	0.0	2.0182717020431252E-4	1.7299471731798216E-4
14-15	5.766490577266072E-5	0.0	0.0	2.450758495338081E-4	2.5949207597697325E-4
16-17	5.766490577266072E-5	0.0	0.0	2.5949207597697325E-4	2.883245288633036E-4
18-19	8.649735865899108E-5	0.0	0.0	2.739083024201384E-4	3.459894346359643E-4
20-21	1.1532981154532144E-4	0.0	0.0	3.892381139654599E-4	3.748218875222947E-4
22-23	1.1532981154532144E-4	0.0	0.0	6.631464163855984E-4	3.892381139654599E-4
24-25	1.441622644316518E-4	0.0	0.0	0.0010668007567942233	4.0365434040862504E-4
26-27	1.441622644316518E-4	0.0	0.0	0.0020182717020431253	4.0365434040862504E-4
28-29	1.7299471731798216E-4	0.0	0.0	0.005838571709481898	4.3248679329495543E-4
30-31	2.0182717020431252E-4	0.0	0.0	0.019000586452091708	4.3248679329495543E-4
32-33	2.0182717020431252E-4	0.0	0.0	0.04953415405871556	4.6131924618128577E-4
34-35	2.0182717020431252E-4	0.0	0.0	0.10183622359451883	4.6131924618128577E-4
36-37	2.0182717020431252E-4	0.0	0.0	0.19130332490080193	4.6131924618128577E-4
38-39	2.5949207597697325E-4	0.0	0.0	0.33832000216820046	4.6131924618128577E-4
40-41	2.5949207597697325E-4	0.0	0.0	0.5177587727062775	4.6131924618128577E-4
42-43	3.3157320819279915E-4	0.0	0.0	0.6891965375683977	4.6131924618128577E-4
44-45	3.748218875222947E-4	0.0	0.0	0.89247974664347	4.901516990676162E-4
46-47	4.180705668517902E-4	0.0	0.0	1.1355661569281215	5.478166048402768E-4
48-49	4.3248679329495543E-4	0.0	0.0	1.3917569170496096	5.766490577266072E-4
50-51	4.3248679329495543E-4	0.0	0.0	1.6643389266369768	5.766490577266072E-4
52-53	4.3248679329495543E-4	0.0	0.0	1.9360703788641973	6.343139634992678E-4
54-55	4.3248679329495543E-4	0.0	0.0	2.240915903231368	6.919788692719286E-4
56-57	4.901516990676162E-4	0.0	0.0	2.600355677138806	6.919788692719286E-4
58-59	4.901516990676162E-4	0.0	0.0	3.0014295130141044	6.919788692719286E-4
60-61	4.901516990676162E-4	0.0	0.0	3.4246322564796614	6.919788692719286E-4
62-63	4.901516990676162E-4	0.0	0.0	3.850934488630499	7.496437750445894E-4
64-65	4.901516990676162E-4	0.0	0.0	4.2998990287499925	7.640600014877546E-4
66-67	5.189841519539465E-4	0.0	0.0	4.811112834651072	7.784762279309197E-4
68-69	5.478166048402768E-4	0.0	0.0	5.394234778050661	7.784762279309197E-4
70-71	6.198977370561028E-4	0.0	0.0	6.03019219136445	7.784762279309197E-4
72-73	6.487301899424331E-4	0.0	0.0	6.673372134126264	9.082222659194064E-4
74-75	6.631464163855983E-4	0.0	0.0	7.311852387067606	9.226384923625715E-4
76-77	6.775626428287635E-4	0.0	0.0	8.023148999773376	9.226384923625715E-4
78-79	7.208113221582591E-4	0.0	0.0	8.816646935658074	9.226384923625715E-4
80-81	8.073086808172501E-4	0.0	0.0	9.663902979949334	9.370547188057367E-4
82-83	8.073086808172501E-4	0.0	0.0	10.526988040875192	9.803033981352323E-4
84-85	8.505573601467456E-4	0.0	0.0	11.38443635726177	9.803033981352323E-4
86-87	8.649735865899109E-4	0.0	0.0	12.28338898957822	9.947196245783975E-4
88-89	8.649735865899109E-4	0.0	0.0	13.257147420908257	0.0010091358510215627
90-91	8.938060394762412E-4	0.0	0.0	14.294510243305538	0.0010091358510215627
92-93	8.938060394762412E-4	0.0	0.0	15.33804321062049	0.0010091358510215627
94-95	8.938060394762412E-4	0.0	0.0	16.37766938056935	0.001037968303907893
96-97	8.938060394762412E-4	0.0	0.0	17.4263777731774	0.0010523845303510582
98-99	9.226384923625715E-4	0.0	0.0	18.534005283258665	0.0010668007567942233
100-101	9.370547188057367E-4	0.0	0.0	19.72396386255685	0.0010668007567942233
102-103	9.514709452489019E-4	0.0	0.0	20.929189225658348	0.0010956332096805537
104-105	0.001037968303907893	0.0	0.0	22.095216869060877	0.0010956332096805537
106-107	0.001037968303907893	1.441622644316518E-5	0.0	23.252018127539777	0.0010956332096805537
108-109	0.001037968303907893	2.883245288633036E-5	0.0	24.413533492065596	0.0011100494361237188
110-111	0.0010812169832373885	4.324867932949554E-5	0.0	25.63373731444154	0.0011677143418963795
112-113	0.0010956332096805537	5.766490577266072E-5	0.0	26.888295004431548	0.001182130568339545
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGG	14150	0.0	30.20096	2
GTACATG	14490	0.0	30.04769	1
GTATCAA	8430	0.0	28.653921	1
CATGGGG	8785	0.0	27.846563	4
ACATGGG	15090	0.0	27.729218	3
TCAACGC	9330	0.0	25.007826	4
AACGCAG	9350	0.0	24.824148	6
CAACGCA	9520	0.0	24.383326	5
ATCAACG	9755	0.0	23.9183	3
TATCAAC	10175	0.0	23.339481	2
GGTATCA	6485	0.0	21.980852	1
ACGCAGA	10410	0.0	21.95182	7
CGCAGAG	10725	0.0	21.195496	8
GTATAGC	1245	0.0	21.07851	1
TACCGTA	325	1.454282E-8	20.14185	7
GTGTAGC	2555	0.0	18.674788	1
ATGGGGA	4635	0.0	18.363337	5
GCAGAGT	11920	0.0	18.321766	9
ACCGTCG	360	4.7022695E-8	18.18309	8
ATGGGGG	4670	0.0	17.078636	5
>>END_MODULE