FastQCFastQC Report
Mon 15 Aug 2016
SRR2921102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468314
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65880.18994819961514442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.16091391955860976No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT44570.12850624251437442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.11622361758479768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75400.041.2195241
GTATCAA113050.040.736471
TCAACGC144900.031.544094
CAACGCA146300.031.2422335
ATCAACG146300.031.1607673
TATCAAC149750.030.3632832
AACGCAG149850.030.2285546
GTACATG144100.028.2330021
TACATGG142500.028.2279872
ACGCAGA169850.026.6879867
ACATGGG149900.026.2381573
CATGGGG89650.026.0571424
CGCAGAG175650.025.773628
GAGTACT98450.023.29351612-13
GCAGAGT190650.023.2452059
AGTACTT105400.022.88635412-13
AGAGTAC173800.021.92199710-11
GTACTTT110600.020.92466214-15
CAGAGTA179500.020.51336710-11
GTATAAT14500.019.7473961
Produced by FastQC (version 0.11.5)