FastQCFastQC Report
Mon 15 Aug 2016
SRR2921101_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921101_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178446
Sequences flagged as poor quality0
Sequence length125
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG31250.036.1833234
GTACATG49150.034.2164881
TACATGG50400.032.706622
ACATGGG51550.030.9393373
ATGGGGG17250.026.5647965
ATAGTCG1150.001003641225.8748043
GTACTAT2151.2051169E-724.9639841
GTATAGA3351.8189894E-1224.9225851
GTATAGC2651.3424142E-924.7546391
GAATATA1951.435983E-624.4661251
GTATAGT2251.8701394E-723.8544711
TATGCCG1250.001631627623.8048175
GTATAGG2102.7353617E-622.7185441
TATAAGT2102.7853166E-622.6712554
ATGGGGC11550.022.1559985
ATGGGGA16700.022.0942925
GTATAAG3859.094947E-1221.6858841
GTCCTAG4450.021.4422231
TCTATAC2306.128661E-620.6998423
GTATTAC2055.8335678E-520.3635751
Produced by FastQC (version 0.11.5)