##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921101_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1178446
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.464361540537283	31.0	31.0	34.0	27.0	34.0
2	30.696448543251027	31.0	31.0	34.0	27.0	34.0
3	30.785704224037417	33.0	31.0	34.0	27.0	34.0
4	34.250490900728586	37.0	35.0	37.0	30.0	37.0
5	34.32780373474898	37.0	35.0	37.0	30.0	37.0
6	34.327632322567176	37.0	35.0	37.0	30.0	37.0
7	34.226243714179525	37.0	35.0	37.0	30.0	37.0
8	34.177977607798745	37.0	35.0	37.0	30.0	37.0
9	35.76917737427086	39.0	37.0	39.0	30.0	39.0
10-11	35.73146372426059	39.0	37.0	39.0	30.0	39.0
12-13	35.66343472675031	39.0	37.0	39.0	30.0	39.0
14-15	36.932053738567575	40.0	37.0	41.0	30.0	41.0
16-17	36.88055031796111	40.0	37.0	41.0	30.0	41.0
18-19	36.8959816571994	40.0	37.0	41.0	30.0	41.0
20-21	36.91589729185724	40.0	37.0	41.0	30.0	41.0
22-23	36.81757840410167	40.0	37.0	41.0	30.0	41.0
24-25	36.77205998408073	40.0	37.0	41.0	30.0	41.0
26-27	36.69632719700351	40.0	37.0	41.0	30.0	41.0
28-29	36.56138422973984	40.0	37.0	41.0	30.0	41.0
30-31	36.46318499108148	40.0	36.5	41.0	29.0	41.0
32-33	36.351571900621664	40.0	36.0	41.0	29.0	41.0
34-35	36.24432430505938	40.0	36.0	41.0	28.5	41.0
36-37	36.140492224505834	40.0	36.0	41.0	28.0	41.0
38-39	36.01951001573258	40.0	36.0	41.0	27.5	41.0
40-41	35.894886146671126	40.0	35.5	41.0	27.0	41.0
42-43	35.73460005804254	40.0	35.0	41.0	27.0	41.0
44-45	35.57026117446196	40.0	35.0	41.0	26.5	41.0
46-47	35.40691215380255	39.0	35.0	41.0	26.0	41.0
48-49	35.18336478718584	39.0	35.0	41.0	26.0	41.0
50-51	35.00923801345161	39.0	35.0	41.0	25.0	41.0
52-53	34.818494440984146	39.0	34.0	41.0	24.5	41.0
54-55	34.58724413337565	38.0	34.0	40.0	24.0	41.0
56-57	34.32275174254909	38.0	34.0	40.0	23.0	41.0
58-59	34.02396248958374	38.0	33.5	40.0	23.0	41.0
60-61	33.69810411338322	37.0	33.0	40.0	22.0	41.0
62-63	33.38096908980131	36.5	33.0	40.0	22.0	41.0
64-65	33.03000689043028	36.0	32.5	39.0	21.0	41.0
66-67	32.647942714388265	35.5	32.0	39.0	20.0	40.5
68-69	32.24337135515755	35.0	32.0	38.5	19.5	40.0
70-71	31.843445520626318	35.0	31.5	37.5	18.0	40.0
72-73	31.454758639767967	35.0	31.0	37.0	18.0	39.0
74-75	31.39908871513841	35.0	31.0	36.5	18.0	39.0
76-77	31.4481961837878	35.0	32.0	36.0	19.0	39.0
78-79	31.259381846940805	35.0	32.0	36.0	18.0	37.0
80-81	31.02369985557251	35.0	32.0	35.0	17.5	37.0
82-83	30.76613947520718	35.0	32.0	35.0	14.0	36.5
84-85	30.497708847074875	35.0	32.0	35.0	9.0	36.0
86-87	30.32725258518422	35.0	31.5	35.0	7.0	36.0
88-89	30.113909334835874	35.0	31.0	35.0	3.5	35.5
90-91	29.919320868329986	35.0	31.0	35.0	2.0	35.0
92-93	29.741611834568573	35.0	31.0	35.0	2.0	35.0
94-95	29.562706309835157	35.0	31.0	35.0	2.0	35.0
96-97	29.393752450260767	35.0	31.0	35.0	2.0	35.0
98-99	29.20491986904788	35.0	30.0	35.0	2.0	35.0
100-101	28.967894583205343	34.5	30.0	35.0	2.0	35.0
102-103	27.573918533390582	32.5	26.0	34.5	2.0	35.0
104-105	28.45524105474498	34.0	29.0	35.0	2.0	35.0
106-107	28.496666796781525	34.0	29.0	35.0	2.0	35.0
108-109	28.3116782610319	34.0	29.0	35.0	2.0	35.0
110-111	28.080378736064276	34.0	29.0	35.0	2.0	35.0
112-113	27.82275598542487	34.0	28.0	35.0	2.0	35.0
114-115	27.528992418829546	34.0	27.0	35.0	2.0	35.0
116-117	27.223094227482633	34.0	27.0	35.0	2.0	35.0
118-119	26.818345091756434	34.0	25.0	35.0	2.0	35.0
120-121	26.381505813588404	34.0	24.5	35.0	2.0	35.0
122-123	25.86059692170876	34.0	21.5	35.0	2.0	35.0
124-125	24.306664879001666	32.5	9.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28692.0
3	4542.0
4	3128.0
5	3491.0
6	4985.0
7	4908.0
8	4587.0
9	4850.0
10	5040.0
11	5108.0
12	5364.0
13	5344.0
14	5303.0
15	5071.0
16	4582.0
17	4556.0
18	4564.0
19	5009.0
20	5647.0
21	6358.0
22	7669.0
23	9440.0
24	11518.0
25	13368.0
26	15565.0
27	17609.0
28	20379.0
29	23690.0
30	28347.0
31	34609.0
32	43263.0
33	55490.0
34	76222.0
35	111696.0
36	206295.0
37	306082.0
38	76034.0
39	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05222734254993	16.909421261132458	10.659586147031415	26.3787652492862
2	17.810150176415892	20.353256369866212	35.02494448525197	26.811648968465924
3	20.119455765443433	23.00504370674654	28.296307368024138	28.579193159785888
4	13.96423099527451	15.0243238744263	33.94980992726618	37.06163520303301
5	14.820439513584354	36.7692787258132	33.31277864554799	15.097503115054439
6	35.62439356786857	33.448884674663496	16.23768231197843	14.689039445489504
7	30.049377010295093	30.8507764220528	20.124954760395763	18.97489180725635
8	27.95929220982234	32.874454578819304	19.45199613957345	19.714257071784903
9	27.308318075810416	14.213327782568616	18.020755895209494	40.45759824641147
10-11	26.513431441252045	24.610689940753012	26.82346945276167	22.05240916523327
12-13	26.756505173648236	22.19823357898572	26.46132312560886	24.583938121757182
14-15	24.234623779124856	23.655195172263536	23.61289429324617	28.497286755365437
16-17	24.616557479746444	26.16595704016734	24.561854301823086	24.655631178263132
18-19	24.692232556164267	25.850384337707567	25.379921009045738	24.07746209708243
20-21	25.674461901985325	25.110617824811264	24.838994211223458	24.375926061979953
22-23	25.266632075335504	24.991146940408083	24.548069353637484	25.194151630618933
24-25	24.866275415739498	25.030617791442307	24.892434305515447	25.210672487302748
26-27	24.7585725947086	25.122720465755915	24.93682412430804	25.181882815227446
28-29	24.838007730783247	25.378236766490765	24.94018452977014	24.84357097295585
30-31	25.080621120228024	25.4414945885966	24.547973919269317	24.929910371906054
32-33	24.66514396961854	25.508898774056167	24.7143244228092	25.111632833516097
34-35	24.988183373656376	25.26660135140587	24.875431736846597	24.869783538091156
36-37	24.89626150721169	25.058998547172717	24.990497804621274	25.05424214099432
38-39	24.768341784933874	25.200617585387207	25.046093848859734	24.984946780819183
40-41	24.837888948674426	25.116012015052362	25.10102080380921	24.945078232464
42-43	24.568060604001765	25.032059992526413	25.078260352617455	25.321619050854366
44-45	24.624124003410568	24.88280414024098	25.296683866716034	25.196387989632413
46-47	24.37329796294771	24.864070555088045	25.44977934551766	25.312852136446583
48-49	24.30148953858356	24.93981469294242	25.444861766981177	25.31383400149285
50-51	24.08106889403876	25.067447016833516	25.50834712561492	25.343136963512798
52-53	24.198699029835343	24.908338422104432	25.294943474298666	25.598019073761556
54-55	24.056495601197927	24.715933702858756	25.438266435416008	25.789304260527313
56-57	23.96850865897207	24.784502529568258	25.441442788267153	25.805546023192523
58-59	23.906701578283016	24.460775593230704	25.62356678460022	26.008956043886062
60-61	24.018202325059963	24.312614231649896	25.716729288825174	25.95245415446497
62-63	23.735011646696353	24.261947684356482	25.745462884516158	26.257577784431014
64-65	23.699354465028073	24.283062116923276	25.75129111240767	26.266292305640977
66-67	23.658624200770927	24.298474532946198	25.611549831385915	26.43135143489696
68-69	23.635835531229947	24.154880264707295	25.631416665569667	26.577867538493095
70-71	23.337356058759536	23.884712822416066	25.918000946090668	26.859930172733737
72-73	23.24760517468225	23.714449108449507	26.03992288329199	26.99802283357625
74-75	23.055636952007237	23.78718579200163	26.188395272494088	26.96878198349705
76-77	22.975866042030045	23.720563022344308	26.271091784805296	27.032479150820354
78-79	22.99442942473057	23.727109326202	26.264461988753546	27.013999260313888
80-81	22.878360570942995	23.514558315861713	26.30637968617755	27.300701427017742
82-83	22.792086314130945	23.438423038619245	26.46727439900962	27.302216248240185
84-85	22.76799062245175	23.441959431910846	26.34713067409622	27.44291927154118
86-87	22.621807872753152	23.319684940395504	26.577845948966733	27.480661237884608
88-89	22.778284189248915	23.064907892787197	26.488640980778676	27.668166937185212
90-91	22.877346371360392	23.029888909792238	26.388330325940768	27.704434392906602
92-93	22.856832314222853	22.753406965162302	26.620691353454006	27.76906936716084
94-95	22.598984745704406	22.643067150072557	26.80622130980799	27.951726794415038
96-97	22.655191405103825	22.53115632878255	26.736629073974754	28.077023192138867
98-99	22.806467789073746	22.56843290588111	26.54339465937361	28.08170464567153
100-101	22.69955506972924	22.6688936537239	26.6238341123966	28.007717164150264
102-103	22.53992325700074	22.57974674300775	26.762061888584242	28.118268111407264
104-105	22.211400022333173	22.582232561338735	26.80073928740201	28.405628128926086
106-107	22.1374593650005	22.728081518427214	26.915477077719814	28.218982038852474
108-109	22.270353095161955	23.00773222473777	26.729409506385338	27.99250517371494
110-111	22.343264428029926	23.35854114061931	26.41745080103583	27.880743630314935
112-113	22.31467457465478	23.485459348210917	26.352614574323578	27.84725150281072
114-115	22.0235213581134	23.715472592954974	26.464797799894342	27.796208249037285
116-117	21.96145025055936	23.89815242994381	26.32467787803549	27.81571944146134
118-119	22.050730926859757	24.083560217605086	26.1999531228504	27.665755732684755
120-121	22.153459038285757	24.445991498095395	26.012659410711315	27.387890052907537
122-123	22.137786123716367	24.762680065465226	25.82916531837859	27.270368492439818
124-125	22.218040769447718	25.007305743673736	25.47585026963291	27.29880321724563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	282.0
1	232.0
2	162.0
3	146.0
4	126.5
5	93.5
6	83.5
7	87.5
8	93.5
9	90.5
10	95.5
11	106.5
12	105.0
13	109.5
14	134.0
15	153.0
16	164.5
17	201.0
18	224.0
19	257.5
20	305.5
21	329.5
22	401.5
23	533.0
24	634.0
25	763.0
26	971.5
27	1270.5
28	1563.0
29	1915.0
30	2369.0
31	2887.0
32	3636.5
33	4662.0
34	5762.0
35	7126.0
36	9198.0
37	11625.5
38	14276.0
39	17234.0
40	20375.5
41	23942.5
42	28017.5
43	32935.5
44	37715.5
45	42218.0
46	47491.5
47	52179.5
48	56557.5
49	60392.5
50	63117.5
51	64328.0
52	63614.5
53	62735.5
54	60992.5
55	57673.5
56	53005.0
57	47746.5
58	42784.5
59	37167.5
60	31050.0
61	25033.5
62	19443.5
63	14678.5
64	10824.0
65	7916.0
66	5751.0
67	4362.5
68	3379.0
69	2586.5
70	2023.5
71	1477.0
72	1101.5
73	896.5
74	726.5
75	562.0
76	396.5
77	258.0
78	171.0
79	130.0
80	90.5
81	60.0
82	47.0
83	39.0
84	31.0
85	21.0
86	18.0
87	17.0
88	13.0
89	8.5
90	5.5
91	4.5
92	5.5
93	7.0
94	6.5
95	3.0
96	1.0
97	4.5
98	7.5
99	8.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28775183589235315
2	0.07051659558435432
3	0.07968120728484801
4	0.08502723077680267
5	0.09325840980409794
6	0.12558912330306182
7	0.11710337172853062
8	0.11659422663405875
9	0.12143110503154153
10-11	0.09936815093776041
12-13	0.0879972438278886
14-15	0.09890143460116119
16-17	0.1004288698845768
18-19	0.09300383725686201
20-21	0.09177340327855497
22-23	0.07526861646609179
24-25	0.08706381115469015
26-27	0.09987729603223228
28-29	0.09122182942621045
30-31	0.08697895363894485
32-33	0.09750128559136355
34-35	0.09160368824706434
36-37	0.09219769085728154
38-39	0.08120864256826363
40-41	0.09245226340451748
42-43	0.08197236020997144
44-45	0.0789174896431402
46-47	0.0689891603009387
48-49	0.07068631061584493
50-51	0.0725531759622418
52-53	0.08630009351298236
54-55	0.09164611700493701
56-57	0.0992832934220151
58-59	0.06835272893284886
60-61	0.08604552096574641
62-63	0.09109454315259248
64-65	0.08256636282018863
66-67	0.0767960517495074
68-69	0.08439079940871283
70-71	0.08222693275720738
72-73	0.0636855655668567
74-75	0.09173097452068232
76-77	0.09516770390836746
78-79	0.07802648572781443
80-81	0.08999139544790342
82-83	0.09491313136113153
84-85	0.09894386335903384
86-87	0.08638495102872766
88-89	0.06529785836601762
90-91	0.09075511308961123
92-93	0.06661314986006996
94-95	0.09380998365644247
96-97	0.08116621381039096
98-99	0.07531104522396444
100-101	0.09083997060535655
102-103	0.06355827929323873
104-105	0.07004987924775509
106-107	0.08854881768023312
108-109	0.09338569607771591
110-111	0.08765781376490735
112-113	0.07658390796014412
114-115	0.08893067650108703
116-117	0.08320279418827846
118-119	0.07637176417078084
120-121	0.11014505543741504
122-123	0.08714866867043547
124-125	0.10946619531145255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1178446.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.34017141242638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05336008974045	43.943390813913325
2	13.891802516194417	16.486838850771054
3	5.207876215383592	9.27108801946682
4	2.503103350705085	5.941383275754342
5	1.3218499432354958	3.92194011065502
6	0.8023119297233667	2.8565596461611507
7	0.5340086980220465	2.2181717373448415
8	0.3423094141293824	1.6250159448419839
9	0.25926731639789713	1.38464702970219
>10	1.0460919432181546	10.03245893997821
>50	0.027131244926959088	1.0791851620664703
>100	0.01060083101473366	1.0631010331254693
>500	0.0	0.0
>1k	2.8650730829698367E-4	0.17621943621917743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	4.2428757872656024E-5
14-15	0.0	0.0	0.0	0.0	8.485751574531205E-5
16-17	0.0	0.0	0.0	4.2428757872656024E-5	8.485751574531205E-5
18-19	0.0	0.0	0.0	8.485751574531205E-5	8.485751574531205E-5
20-21	0.0	0.0	0.0	3.394300629812482E-4	8.485751574531205E-5
22-23	0.0	0.0	0.0	7.212888838351525E-4	8.485751574531205E-5
24-25	0.0	0.0	0.0	0.0012304339783070248	8.485751574531205E-5
26-27	0.0	0.0	0.0	0.002079009135760145	8.485751574531205E-5
28-29	0.0	0.0	0.0	0.018414080916732713	8.485751574531205E-5
30-31	0.0	0.0	0.0	0.07552318901332772	8.485751574531205E-5
32-33	0.0	0.0	0.0	0.1736609059727811	8.485751574531205E-5
34-35	0.0	0.0	0.0	0.33166560029055214	8.485751574531205E-5
36-37	0.0	0.0	0.0	0.5975666258784874	8.485751574531205E-5
38-39	0.0	0.0	0.0	1.0060707066764196	8.485751574531205E-5
40-41	0.0	0.0	0.0	1.451063519244836	8.485751574531205E-5
42-43	0.0	0.0	0.0	1.8760299580973587	8.485751574531205E-5
44-45	0.0	0.0	0.0	2.395994385826758	8.485751574531205E-5
46-47	0.0	0.0	0.0	3.033231900316179	8.485751574531205E-5
48-49	0.0	0.0	0.0	3.692023223804909	8.485751574531205E-5
50-51	0.0	0.0	0.0	4.377205234690431	8.485751574531205E-5
52-53	0.0	0.0	0.0	5.064211682164478	8.485751574531205E-5
54-55	0.0	0.0	0.0	5.844476539442622	8.485751574531205E-5
56-57	0.0	0.0	0.0	6.7625924310490255	8.485751574531205E-5
58-59	0.0	0.0	0.0	7.74829733394657	8.485751574531205E-5
60-61	0.0	0.0	0.0	8.778170573789549	8.485751574531205E-5
62-63	0.0	0.0	0.0	9.780083262194449	8.485751574531205E-5
64-65	0.0	0.0	0.0	10.84593608871344	8.485751574531205E-5
66-67	0.0	0.0	0.0	12.057064982188407	8.485751574531205E-5
68-69	0.0	0.0	0.0	13.408972494284846	8.485751574531205E-5
70-71	0.0	0.0	0.0	14.843149367896364	8.485751574531205E-5
72-73	0.0	0.0	0.0	16.217289549118078	1.2728627361796807E-4
74-75	0.0	0.0	0.0	17.57785252782054	1.697150314906241E-4
76-77	0.0	0.0	0.0	19.091116606106688	1.697150314906241E-4
78-79	0.0	0.0	0.0	20.74375915400451	1.697150314906241E-4
80-81	0.0	4.2428757872656024E-5	0.0	22.447528355138886	2.1214378936328012E-4
82-83	0.0	8.485751574531205E-5	0.0	24.128470884537773	2.5457254723593615E-4
84-85	0.0	8.485751574531205E-5	0.0	25.76804537501082	2.5457254723593615E-4
86-87	0.0	8.485751574531205E-5	0.0	27.4621832481081	2.5457254723593615E-4
88-89	0.0	8.485751574531205E-5	0.0	29.308555504452475	2.5457254723593615E-4
90-91	0.0	8.485751574531205E-5	0.0	31.186664471685596	2.5457254723593615E-4
92-93	0.0	8.485751574531205E-5	0.0	33.0474200769488	2.5457254723593615E-4
94-95	0.0	8.485751574531205E-5	0.0	34.82526988932883	2.5457254723593615E-4
96-97	0.0	8.485751574531205E-5	0.0	36.60231355530928	2.5457254723593615E-4
98-99	0.0	8.485751574531205E-5	0.0	38.464596595855895	2.5457254723593615E-4
100-101	0.0	8.485751574531205E-5	0.0	40.42039261875385	2.5457254723593615E-4
102-103	0.0	8.485751574531205E-5	0.0	42.3265469949408	2.5457254723593615E-4
104-105	0.0	8.485751574531205E-5	0.0	44.10176622433272	2.5457254723593615E-4
106-107	0.0	8.485751574531205E-5	0.0	45.83646598995627	2.5457254723593615E-4
108-109	0.0	8.485751574531205E-5	0.0	47.58724625481354	2.5457254723593615E-4
110-111	0.0	1.2728627361796807E-4	0.0	49.370569376959146	2.5457254723593615E-4
112-113	0.0	1.697150314906241E-4	0.0	51.15783837358691	2.5457254723593615E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	3125	0.0	36.183323	4
GTACATG	4915	0.0	34.216488	1
TACATGG	5040	0.0	32.70662	2
ACATGGG	5155	0.0	30.939337	3
ATGGGGG	1725	0.0	26.564796	5
ATAGTCG	115	0.0010036412	25.874804	3
GTACTAT	215	1.2051169E-7	24.963984	1
GTATAGA	335	1.8189894E-12	24.922585	1
GTATAGC	265	1.3424142E-9	24.754639	1
GAATATA	195	1.435983E-6	24.466125	1
GTATAGT	225	1.8701394E-7	23.854471	1
TATGCCG	125	0.0016316276	23.804817	5
GTATAGG	210	2.7353617E-6	22.718544	1
TATAAGT	210	2.7853166E-6	22.671255	4
ATGGGGC	1155	0.0	22.155998	5
ATGGGGA	1670	0.0	22.094292	5
GTATAAG	385	9.094947E-12	21.685884	1
GTCCTAG	445	0.0	21.442223	1
TCTATAC	230	6.128661E-6	20.699842	3
GTATTAC	205	5.8335678E-5	20.363575	1
>>END_MODULE