FastQCFastQC Report
Mon 15 Aug 2016
SRR2921101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178446
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT18560.15749554922329917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG31900.031.3840374
GTACATG50450.029.5580251
TACATGG50300.029.2630232
ACATGGG49550.028.623553
ATGGGGG15950.026.9006045
GTATAGT1451.317678E-424.6819691
GTATCAA35050.024.67611
GGTATCA29150.024.5549621
GTATATG2052.2148142E-623.2773041
GTATAGG1802.1356556E-523.1964821
GTATAGA2403.4787809E-722.3680341
GTCCTAA2204.088495E-621.6902161
TAACACG1400.003127460421.2829694
GTATAAT3401.036824E-921.0522691
TACTACT2607.581639E-720.6281074
CTAGTAT1754.7337587E-420.4316483
TATCAAC42000.020.005992
CAACGCA41300.019.9121995
GCATAAG2701.0793683E-619.88271
TCAACGC41700.019.7211954
Produced by FastQC (version 0.11.5)