##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1178446
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.497695269872356	33.0	31.0	34.0	28.0	34.0
2	31.550805891827032	33.0	31.0	34.0	28.0	34.0
3	31.670408317394262	34.0	31.0	34.0	28.0	34.0
4	35.18387775086852	37.0	35.0	37.0	32.0	37.0
5	34.96263384151671	37.0	35.0	37.0	32.0	37.0
6	34.77744843633056	37.0	35.0	37.0	32.0	37.0
7	34.72292748246419	37.0	35.0	37.0	32.0	37.0
8	34.710112300436336	37.0	35.0	37.0	32.0	37.0
9	36.32187304297354	39.0	37.0	39.0	32.0	39.0
10-11	36.2844241484124	39.0	37.0	39.0	32.0	39.0
12-13	36.21788355172829	39.0	37.0	39.0	31.5	39.0
14-15	37.50169842317764	40.0	38.0	41.0	32.0	41.0
16-17	37.47610072926549	40.0	38.0	41.0	31.5	41.0
18-19	37.47239627441563	40.0	38.0	41.0	31.5	41.0
20-21	37.47459959981195	40.0	38.0	41.0	31.0	41.0
22-23	37.438952654597664	40.0	38.0	41.0	31.0	41.0
24-25	37.40077483397627	40.0	37.5	41.0	31.0	41.0
26-27	37.29914438166874	40.0	37.0	41.0	31.0	41.0
28-29	37.19521556354725	40.0	37.0	41.0	30.5	41.0
30-31	37.09434373743048	40.0	37.0	41.0	30.0	41.0
32-33	37.0211172170808	40.0	37.0	41.0	30.0	41.0
34-35	36.89926734020905	40.0	37.0	41.0	30.0	41.0
36-37	36.74186725569097	40.0	36.5	41.0	30.0	41.0
38-39	36.62334506629918	40.0	36.0	41.0	30.0	41.0
40-41	36.51189065939381	40.0	36.0	41.0	30.0	41.0
42-43	36.39434178570762	40.0	36.0	41.0	29.5	41.0
44-45	36.25443125947222	40.0	35.5	41.0	29.0	41.0
46-47	36.058465131198204	40.0	35.0	41.0	28.0	41.0
48-49	35.8601773012934	39.5	35.0	41.0	28.0	41.0
50-51	35.68889877007517	39.0	35.0	41.0	27.5	41.0
52-53	35.48368868832344	39.0	35.0	41.0	27.0	41.0
54-55	35.271038299591154	39.0	35.0	41.0	26.5	41.0
56-57	35.009762432898924	38.5	34.5	41.0	26.0	41.0
58-59	34.74317830430923	38.0	34.0	40.0	26.0	41.0
60-61	34.43553629101376	37.5	34.0	40.0	26.0	41.0
62-63	34.11284649445116	37.0	34.0	40.0	26.0	41.0
64-65	33.771038299591154	36.5	34.0	39.5	25.0	41.0
66-67	33.39104464693334	36.0	33.0	39.0	24.0	41.0
68-69	32.99770163418604	35.0	33.0	39.0	23.0	40.5
70-71	32.569092262182565	35.0	33.0	37.5	22.5	40.0
72-73	32.15942648199409	35.0	32.0	37.0	21.5	39.0
74-75	31.829516159416723	35.0	32.0	36.5	20.5	39.0
76-77	31.529110795064007	35.0	32.0	36.0	20.0	38.5
78-79	31.242083218068544	35.0	32.0	35.5	20.0	37.0
80-81	31.225608555674167	35.0	32.0	35.0	20.0	37.0
82-83	31.04546962694939	35.0	32.0	35.0	20.0	36.5
84-85	30.865706192731786	35.0	32.0	35.0	19.5	36.0
86-87	30.715453232477348	35.0	32.0	35.0	19.0	36.0
88-89	30.557431990944004	35.0	32.0	35.0	18.0	35.5
90-91	30.40685614784216	35.0	31.5	35.0	17.0	35.0
92-93	30.30748926976713	35.0	31.0	35.0	16.0	35.0
94-95	30.21267330026153	35.0	31.0	35.0	15.0	35.0
96-97	30.14506392316661	35.0	31.0	35.0	11.5	35.0
98-99	30.04955339489463	35.0	31.0	35.0	9.0	35.0
100-101	29.941830597244167	35.0	31.0	35.0	7.0	35.0
102-103	28.51697956461306	32.5	28.0	34.5	7.0	35.0
104-105	29.588712592685624	34.0	30.5	35.0	6.0	35.0
106-107	29.75070643881858	34.5	31.0	35.0	2.0	35.0
108-109	29.719045251118846	35.0	31.0	35.0	2.0	35.0
110-111	29.568754529269903	34.5	31.0	35.0	2.0	35.0
112-113	29.376308715036583	34.0	30.5	35.0	2.0	35.0
114-115	29.202220127184443	34.0	30.0	35.0	2.0	35.0
116-117	29.00150664519206	34.0	30.0	35.0	2.0	35.0
118-119	28.75217489812855	34.0	30.0	35.0	2.0	35.0
120-121	28.790718454642807	35.0	30.5	35.0	2.0	35.0
122-123	28.57388586324702	35.0	30.5	35.0	2.0	35.0
124-125	27.732426008489146	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14638.0
3	5779.0
4	2845.0
5	2554.0
6	2964.0
7	3336.0
8	3905.0
9	4401.0
10	4945.0
11	5643.0
12	5684.0
13	6418.0
14	7169.0
15	6704.0
16	4778.0
17	4508.0
18	4525.0
19	4644.0
20	4992.0
21	5447.0
22	5763.0
23	5732.0
24	7097.0
25	8924.0
26	11532.0
27	14101.0
28	17292.0
29	20657.0
30	25629.0
31	31597.0
32	39800.0
33	51798.0
34	71098.0
35	105843.0
36	206704.0
37	350865.0
38	98036.0
39	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.160758380048534	17.4211508302485	10.957750190190021	26.460340599512943
2	18.010620592611076	20.39045148127308	34.85054872496026	26.748379201155586
3	20.11191009176492	23.05858732602088	28.718923056296177	28.110579525918034
4	14.184220218364995	15.203064509832876	33.81229028661042	36.80042498519171
5	14.799918078968815	36.60586891105365	33.62237973797168	14.971833272005867
6	35.68348977170726	33.60004144137871	15.983464210528997	14.733004576385039
7	29.962804267962024	30.640949590151067	20.207474619407805	19.188771522479104
8	28.2486930545183	32.61787969312241	19.550716274017244	19.582710978342046
9	27.445402413470898	13.546261021146513	18.251078033409062	40.75725853197352
10-11	26.712220945039117	24.25548923538315	27.030837819385944	22.00145200019179
12-13	27.036873749025638	21.668265679055512	26.847326070804357	24.447534501114497
14-15	24.38822568900461	23.029863514538818	24.029104790485224	28.55280600597134
16-17	24.59637345056885	25.81529465183683	24.937386219451646	24.650945678142676
18-19	24.702979120692582	25.28301646579528	25.94279409268376	24.07121032082838
20-21	25.506696396363516	24.565534205136604	25.520955786553905	24.406813611945974
22-23	25.18585352997571	24.555243813264152	25.32815344114967	24.930749215610465
24-25	24.859811100949162	24.5632425619142	25.67032426043539	24.90662207670125
26-27	24.784635226246003	24.57320662329169	25.779775593703995	24.86238255675831
28-29	24.913373222320914	24.82298006300361	25.647891908454223	24.615754806221258
30-31	24.96371448263218	24.867250177395864	25.469207812887255	24.699827527084697
32-33	24.72426435482591	24.89809377537046	25.475134456802156	24.902507413001473
34-35	24.944776856059946	24.64431202678381	25.695981367785503	24.714929749370743
36-37	24.91523920389995	24.55658931638386	25.681131349208535	24.847040130507647
38-39	24.787868114333726	24.490851646661163	25.828083524476593	24.893196714528514
40-41	24.78645355664588	24.44986353483607	25.837814622122423	24.925868286395627
42-43	24.65986719341153	24.318195996450456	25.73118102971576	25.290755780422252
44-45	24.642804392962702	24.33921359244728	25.886877305685758	25.131104708904257
46-47	24.46283790665933	24.262648794366694	25.96211303279078	25.312400266183193
48-49	24.362237644720302	24.3182730574315	25.96074929915525	25.358739998692947
50-51	24.034849634461604	24.58718858793715	25.98738650656645	25.390575271034795
52-53	24.138293935721293	24.418074694205956	25.918994707378097	25.524636662694654
54-55	23.903344888797225	24.25518606079201	25.951331254792677	25.89013779561809
56-57	23.90616711860235	24.457613524971038	25.705040886388037	25.931178470038574
58-59	23.73160061836605	24.328579315460466	25.95134462364839	25.9884754425251
60-61	23.899217605219302	24.411124544501277	25.847714581433433	25.84194326884599
62-63	23.47484023186536	24.478812156806164	25.82701757661657	26.21933003471191
64-65	23.291505943077006	24.53440202761058	25.76100117516194	26.41309085415048
66-67	23.293778746107417	24.5528996276533	25.67931841966113	26.474003206578157
68-69	23.45943165788799	24.370795614453495	25.52913937299382	26.640633354664693
70-71	23.260763559247586	24.291359317779897	25.63634534888705	26.811531774085466
72-73	23.266252661100566	24.232355248012873	25.628562988717274	26.872829102169288
74-75	23.10585131979967	24.367216721111955	25.61576647341347	26.911165485674903
76-77	23.116653208405594	24.278547390405695	25.592520629753267	27.012278771435444
78-79	23.135326205727406	24.413033376970294	25.4664310129362	26.9852094043661
80-81	22.895626324801558	24.44717449930723	25.420997799688944	27.236201376202267
82-83	22.78608050338592	24.366927191853076	25.47631636793546	27.370675936825545
84-85	22.825670445243453	24.20243994244082	25.293705026583567	27.67818458573216
86-87	22.683371346735267	24.39242560865645	25.44303824325041	27.48116480135787
88-89	22.812927560439856	24.209639143232536	25.280258972191383	27.69717432413622
90-91	22.79136835967686	24.161497615312246	25.169775594495718	27.877358430515176
92-93	22.67125249571935	24.14354125867004	25.19453349939636	27.99067274621425
94-95	22.41338778475394	23.991893391994214	25.49974624415895	28.094972579092897
96-97	22.49064684454071	23.940173754808132	25.496557495595017	28.072621905056135
98-99	22.607457578207388	24.030988013469727	25.23795883118068	28.1235955771422
100-101	22.488976033748827	24.07281971624162	25.33801878424432	28.100185465765225
102-103	22.29862375393077	24.095806756888656	25.364091987387592	28.241477501792982
104-105	22.200858953343158	24.419574223591813	25.215555194393254	28.16401162867178
106-107	22.12710356838528	24.70073907095013	25.213242508292055	27.95891485237253
108-109	22.14885480635076	25.013536267011567	24.999660532488736	27.83794839414894
110-111	22.11910204102419	25.383705247826526	24.80692727700196	27.690265434147328
112-113	21.88789368779787	25.644644606839464	24.68896017069158	27.77850153467109
114-115	21.760531883564756	25.872080441108952	24.727425797948406	27.63996187737789
116-117	21.75169435858789	26.095201481428674	24.629048765957013	27.52405539402642
118-119	21.836904247672408	26.327116516727017	24.488464081102343	27.347515154498232
120-121	21.923189852106052	26.559810999887983	24.393505836668332	27.123493311337633
122-123	21.72792696540777	26.85411284194126	24.18159191208761	27.236368280563354
124-125	21.447634160415465	27.226587692203253	24.221598044872884	27.1041801025084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	8.0
2	24.0
3	45.0
4	46.5
5	39.0
6	53.0
7	98.5
8	140.5
9	161.0
10	148.0
11	134.5
12	145.0
13	158.0
14	185.0
15	204.0
16	212.5
17	225.5
18	271.0
19	316.0
20	333.0
21	365.5
22	431.0
23	508.5
24	589.0
25	698.5
26	909.5
27	1253.5
28	1570.0
29	1949.0
30	2421.0
31	2978.0
32	3728.0
33	4757.5
34	5944.5
35	7205.5
36	9149.0
37	11527.0
38	14022.0
39	16802.0
40	20132.5
41	23717.0
42	27614.0
43	32791.5
44	37956.5
45	42486.5
46	47617.0
47	52455.0
48	56988.5
49	61024.5
50	64143.0
51	65632.5
52	64435.0
53	62637.0
54	60548.0
55	57260.0
56	53035.5
57	47829.0
58	42652.5
59	37156.5
60	31039.5
61	25022.5
62	19342.0
63	14489.5
64	10635.0
65	7851.0
66	5701.5
67	4234.5
68	3203.5
69	2340.0
70	1738.5
71	1329.0
72	990.0
73	761.5
74	627.5
75	450.5
76	288.5
77	190.0
78	124.5
79	71.0
80	41.0
81	26.0
82	16.5
83	8.5
84	8.0
85	7.5
86	3.5
87	3.5
88	3.0
89	2.5
90	1.5
91	0.5
92	3.0
93	4.0
94	1.5
95	3.5
96	5.5
97	3.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16895131384891626
2	0.014340920160957737
3	0.0
4	0.004412590818756227
5	0.1445123493142664
6	0.07458975634012929
7	0.007212888838351524
8	0.010692046983909319
9	0.012134624751579623
10-11	0.006067312375789812
12-13	0.009716185552838229
14-15	0.01289834239328743
16-17	0.0162077855073546
18-19	0.020874948873346764
20-21	0.023335816829960814
22-23	0.025499683481466272
24-25	0.025754256028702207
26-27	0.022953958009106906
28-29	0.022020525335908474
30-31	0.024608679566140493
32-33	0.023717675650814715
34-35	0.02303881552485222
36-37	0.023547960619324092
38-39	0.019389942347803803
40-41	0.026008828575938146
42-43	0.02261452794612566
44-45	0.03224585598321858
46-47	0.026305829881046735
48-49	0.01909294104269521
50-51	0.015613782897137416
52-53	0.017141218180553034
54-55	0.018286794643114744
56-57	0.015359210349901481
58-59	0.015231924076283512
60-61	0.01735336196991631
62-63	0.01493492277117492
64-65	0.026348258638919392
66-67	0.02062037632611083
68-69	0.017989793338006155
70-71	0.024820823355503775
72-73	0.030718420699802962
74-75	0.0168866456333171
76-77	0.009461613005602294
78-79	0.013874203824358519
80-81	0.017268504454171
82-83	0.015698640412882728
84-85	0.015316781592028823
86-87	0.011328478351999158
88-89	0.02121437893632801
90-91	0.016929074391189753
92-93	0.0151470665605382
94-95	0.012558912330306184
96-97	0.01985665868440302
98-99	0.02108709266271004
100-101	0.028087837711698285
102-103	0.020450661294620205
104-105	0.012855913635414775
106-107	0.01782007830651553
108-109	0.011031477046890566
110-111	0.02231752664101707
112-113	0.015316781592028823
114-115	0.012134624751579623
116-117	0.010522331952418694
118-119	0.0070431738068609
120-121	0.00288515553534061
122-123	0.003861016966411698
124-125	5.091450944718723E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1178446.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.03931693909693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10902803983286	42.431980495131846
2	14.340129969588103	16.645826965055328
3	5.392884504796191	9.38997998969433
4	2.5860069338904976	6.003603041710916
5	1.4237081142035424	4.13155232345117
6	0.8527949124907785	2.969738052606104
7	0.54244032435945	2.2038006134231263
8	0.3649402819565158	1.6944707750654093
9	0.2630477855963851	1.374040241852059
>10	1.0829485169800626	10.191065179385246
>50	0.02874846938809209	1.1232022052829287
>100	0.012150975493662756	1.1432420179730725
>500	4.3918928396508375E-4	0.18709840599455532
>1k	7.319821399418064E-4	0.5103996933738846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	1856	0.15749554922329917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	4.2428757872656024E-5	0.0
18-19	0.0	0.0	0.0	8.485751574531205E-5	0.0
20-21	0.0	0.0	0.0	3.394300629812482E-4	0.0
22-23	0.0	0.0	0.0	7.212888838351525E-4	0.0
24-25	0.0	0.0	0.0	0.0011880052204343687	0.0
26-27	0.0	0.0	0.0	0.001951722862142177	0.0
28-29	0.0	0.0	0.0	0.018414080916732713	0.0
30-31	0.0	0.0	0.0	0.07552318901332772	0.0
32-33	0.0	0.0	0.0	0.17306690336256392	0.0
34-35	0.0	0.0	0.0	0.3299684499756459	0.0
36-37	0.0	0.0	0.0	0.5942571827644203	0.0
38-39	0.0	0.0	0.0	1.0008943982159555	0.0
40-41	0.0	0.0	0.0	1.443171770280522	0.0
42-43	0.0	0.0	0.0	1.8665683450917565	0.0
44-45	0.0	0.0	0.0	2.384708336232632	4.2428757872656024E-5
46-47	0.0	0.0	0.0	3.0188909801552213	8.485751574531205E-5
48-49	0.0	0.0	0.0	3.674160716740521	8.485751574531205E-5
50-51	0.0	0.0	0.0	4.357136432216665	8.485751574531205E-5
52-53	0.0	0.0	0.0	5.042191156828569	8.485751574531205E-5
54-55	0.0	0.0	0.0	5.823134874232676	8.485751574531205E-5
56-57	0.0	0.0	0.0	6.7439237775850565	1.697150314906241E-4
58-59	0.0	0.0	0.0	7.731665260860489	1.697150314906241E-4
60-61	0.0	0.0	0.0	8.762556790892411	1.697150314906241E-4
62-63	0.0	0.0	0.0	9.770239790367992	1.697150314906241E-4
64-65	0.0	0.0	0.0	10.840293063916377	1.697150314906241E-4
66-67	0.0	0.0	0.0	12.052270532548796	1.697150314906241E-4
68-69	0.0	0.0	0.0	13.404984191044816	1.697150314906241E-4
70-71	0.0	0.0	0.0	14.838948920866972	1.697150314906241E-4
72-73	0.0	0.0	0.0	16.207191504744383	1.697150314906241E-4
74-75	0.0	0.0	0.0	17.562493317470633	1.697150314906241E-4
76-77	0.0	0.0	0.0	19.071090232390794	2.1214378936328012E-4
78-79	0.0	0.0	0.0	20.72585421818225	2.5457254723593615E-4
80-81	0.0	0.0	0.0	22.431532713420893	2.5457254723593615E-4
82-83	0.0	0.0	0.0	24.113451104250853	2.5457254723593615E-4
84-85	0.0	0.0	0.0	25.750522298009415	2.5457254723593615E-4
86-87	0.0	0.0	0.0	27.4423265894237	2.5457254723593615E-4
88-89	0.0	0.0	0.0	29.290183852293616	2.5457254723593615E-4
90-91	0.0	0.0	0.0	31.168547392073968	2.5457254723593615E-4
92-93	0.0	0.0	0.0	33.02917571106356	2.5457254723593615E-4
94-95	0.0	0.0	0.0	34.8071528097172	2.5457254723593615E-4
96-97	0.0	0.0	0.0	36.58398433190829	2.5457254723593615E-4
98-99	0.0	0.0	0.0	38.450934535820906	2.5457254723593615E-4
100-101	0.0	0.0	0.0	40.41097343450612	2.5457254723593615E-4
102-103	0.0	0.0	0.0	42.32315269431098	2.5457254723593615E-4
104-105	0.0	0.0	0.0	44.105499955025515	2.5457254723593615E-4
106-107	0.0	0.0	0.0	45.84070886574353	2.5457254723593615E-4
108-109	0.0	0.0	0.0	47.596241151482545	2.5457254723593615E-4
110-111	0.0	0.0	0.0	49.387074163771615	2.5457254723593615E-4
112-113	0.0	0.0	0.0	51.1797316126492	2.5457254723593615E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	3190	0.0	31.384037	4
GTACATG	5045	0.0	29.558025	1
TACATGG	5030	0.0	29.263023	2
ACATGGG	4955	0.0	28.62355	3
ATGGGGG	1595	0.0	26.900604	5
GTATAGT	145	1.317678E-4	24.681969	1
GTATCAA	3505	0.0	24.6761	1
GGTATCA	2915	0.0	24.554962	1
GTATATG	205	2.2148142E-6	23.277304	1
GTATAGG	180	2.1356556E-5	23.196482	1
GTATAGA	240	3.4787809E-7	22.368034	1
GTCCTAA	220	4.088495E-6	21.690216	1
TAACACG	140	0.0031274604	21.282969	4
GTATAAT	340	1.036824E-9	21.052269	1
TACTACT	260	7.581639E-7	20.628107	4
CTAGTAT	175	4.7337587E-4	20.431648	3
TATCAAC	4200	0.0	20.00599	2
CAACGCA	4130	0.0	19.912199	5
GCATAAG	270	1.0793683E-6	19.8827	1
TCAACGC	4170	0.0	19.721195	4
>>END_MODULE