FastQCFastQC Report
Mon 15 Aug 2016
SRR2921100_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921100_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311981
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27230.11777778450601455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71400.028.079421
GTACATG108700.027.9954661
TACATGG105300.027.4202522
CATGGGG63200.025.9044114
ACATGGG112900.024.8889273
AACGCAG80600.024.2970286
TATCAAC84100.023.5725382
TCAACGC83850.023.3588224
TTACGTA2052.2538588E-623.2324074
ATCAACG86450.022.65633
CAACGCA86550.022.6296355
GGTATCA55150.021.5305251
ACGCAGA90400.021.5295017
CGCAGAG93400.020.7100778
ATGGGGA31000.020.1639825
GCGTAGA7300.019.6171281
GCAGAGT100050.018.738269
ATGGGGG27750.018.664015
GTATAAA12050.018.3215311
GTATAAT10850.018.1481061
Produced by FastQC (version 0.11.5)