##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921100_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2311981
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.00050778964014	31.0	31.0	34.0	26.0	34.0
2	30.174358266785063	31.0	31.0	34.0	26.0	34.0
3	30.27192913782596	31.0	31.0	34.0	26.0	34.0
4	33.6704531741394	37.0	35.0	37.0	28.0	37.0
5	33.800509606264065	37.0	35.0	37.0	28.0	37.0
6	33.85046589915748	37.0	35.0	37.0	29.0	37.0
7	33.828796603432295	37.0	35.0	37.0	29.0	37.0
8	33.78485030802589	37.0	35.0	37.0	29.0	37.0
9	35.26573877553492	39.0	37.0	39.0	28.0	39.0
10-11	35.25931679369337	39.0	36.0	39.0	28.0	39.0
12-13	35.18537825354101	39.0	35.0	39.0	27.5	39.0
14-15	36.4353221328376	40.0	37.0	41.0	27.5	41.0
16-17	36.41964747980195	40.0	37.0	41.0	27.5	41.0
18-19	36.45612701834487	40.0	37.0	41.0	28.0	41.0
20-21	36.50295871808635	40.0	37.0	41.0	28.0	41.0
22-23	36.45076754523502	40.0	37.0	41.0	28.0	41.0
24-25	36.4413035833772	40.0	37.0	41.0	28.0	41.0
26-27	36.379704244974334	40.0	36.5	41.0	28.5	41.0
28-29	36.246350207895304	40.0	36.0	41.0	28.0	41.0
30-31	36.13953791142747	40.0	36.0	41.0	28.0	41.0
32-33	36.0100392261009	40.0	36.0	41.0	27.0	41.0
34-35	35.881892411745596	40.0	36.0	41.0	27.0	41.0
36-37	35.75445797348681	40.0	35.5	41.0	26.5	41.0
38-39	35.61466119314994	40.0	35.0	41.0	26.0	41.0
40-41	35.476465420779846	40.0	35.0	41.0	25.5	41.0
42-43	35.302751406694085	40.0	35.0	41.0	25.0	41.0
44-45	35.120135286578915	40.0	35.0	41.0	24.0	41.0
46-47	34.93841255615855	39.0	35.0	41.0	24.0	41.0
48-49	34.703476153134474	39.0	34.5	41.0	23.0	41.0
50-51	34.52446516645249	39.0	34.0	41.0	23.0	41.0
52-53	34.34364750402361	39.0	34.0	41.0	22.0	41.0
54-55	34.11805676603744	38.5	34.0	40.5	20.5	41.0
56-57	33.86686395779204	38.0	33.0	40.0	19.5	41.0
58-59	33.583112707241106	38.0	33.0	40.0	18.5	41.0
60-61	33.27689176511399	37.0	33.0	40.0	17.5	41.0
62-63	32.981605385165366	37.0	32.5	40.0	14.0	41.0
64-65	32.650219876374415	36.0	32.0	39.5	12.5	41.0
66-67	32.29434843971468	36.0	32.0	39.0	9.0	41.0
68-69	31.914252539272596	35.0	31.5	39.0	7.5	40.5
70-71	31.53836147442388	35.0	31.0	38.0	7.0	40.0
72-73	31.17283078883434	35.0	31.0	37.0	6.0	39.5
74-75	31.112641496621297	35.0	31.0	37.0	2.0	39.0
76-77	31.144323850412267	35.0	32.0	36.5	2.0	39.0
78-79	30.95867786110699	35.0	32.0	36.0	2.0	39.0
80-81	30.734761661103615	35.0	32.0	36.0	2.0	37.0
82-83	30.484266306686777	35.0	32.0	35.0	2.0	37.0
84-85	30.229918844488772	35.0	31.5	35.0	2.0	36.5
86-87	30.073655665855384	35.0	31.0	35.0	2.0	36.0
88-89	29.893229010099997	35.0	31.0	35.0	2.0	36.0
90-91	29.74545292543494	35.0	31.0	35.0	2.0	36.0
92-93	29.62091340716035	35.0	31.0	35.0	2.0	35.0
94-95	29.491730252108475	35.0	31.0	35.0	2.0	35.0
96-97	29.378174388111322	35.0	31.0	35.0	2.0	35.0
98-99	29.2664840238739	35.0	31.0	35.0	2.0	35.0
100-101	29.120479580065755	35.0	30.0	35.0	2.0	35.0
102-103	27.851905357353715	33.0	28.0	34.5	2.0	35.0
104-105	28.764554726012022	34.0	29.5	35.0	2.0	35.0
106-107	28.87388218155772	35.0	30.0	35.0	2.0	35.0
108-109	28.786185959140667	35.0	30.0	35.0	2.0	35.0
110-111	28.66571589472405	35.0	30.0	35.0	2.0	35.0
112-113	28.523336048176866	35.0	29.5	35.0	2.0	35.0
114-115	28.351066682641424	35.0	29.0	35.0	2.0	35.0
116-117	28.173154104640133	35.0	29.0	35.0	2.0	35.0
118-119	27.929260880604126	34.0	29.0	35.0	2.0	35.0
120-121	27.67859792100368	34.0	29.0	35.0	2.0	35.0
122-123	27.328359316101647	34.0	28.0	35.0	2.0	35.0
124-125	26.017499711286554	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68174.0
3	10135.0
4	7520.0
5	8461.0
6	13917.0
7	13737.0
8	10623.0
9	11091.0
10	12148.0
11	12624.0
12	13247.0
13	13202.0
14	13885.0
15	12109.0
16	8865.0
17	8202.0
18	7880.0
19	7899.0
20	8327.0
21	9061.0
22	10502.0
23	14101.0
24	19058.0
25	23725.0
26	26109.0
27	28476.0
28	33429.0
29	39779.0
30	47580.0
31	57799.0
32	73136.0
33	95289.0
34	131584.0
35	200700.0
36	380604.0
37	636911.0
38	221713.0
39	379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10702402211834	18.168253292137603	11.421447833515586	25.30327485222847
2	16.637466056454706	21.17527550862298	37.121751828861335	25.06550660606097
3	19.41586029126907	24.858683207552478	28.664659655835877	27.060796845342573
4	12.965208263443245	16.72730420855563	35.19633793976647	35.11114958823466
5	13.898220411329662	38.42843116713103	33.45906357306496	14.214284848474353
6	33.18762826239558	35.83114898422895	16.782296790576602	14.198925962798873
7	28.344862464451364	32.78510784962364	20.668955173214083	18.20107451271091
8	26.44562112552928	35.11958602456193	19.685656128640332	18.74913672126846
9	26.52568904670145	16.06400069283565	18.47286032866391	38.93744993179899
10-11	25.115391667123042	26.56364556926953	27.135414175073237	21.185548588534193
12-13	25.51882649392724	24.303797851925633	26.934909736462416	23.24246591768471
14-15	23.247811408017448	25.59936671911538	24.234531255039503	26.918290617827672
16-17	23.288735766342462	28.027155104115348	25.145962364525364	23.53814676501683
18-19	23.399376880689147	27.252725227067987	26.536856637103778	22.811041255139088
20-21	24.47719719601853	26.49762922180553	26.068495208443633	22.956678373732306
22-23	24.086249645355075	26.379609328995574	25.77988880326193	23.754252222387418
24-25	23.62303109663386	26.480068827071985	26.071002267181065	23.825897809113094
26-27	23.50087320629055	26.577810602984226	26.167853886655486	23.75346230406974
28-29	23.51588749565207	26.88563700090281	26.151635372770272	23.446840130674843
30-31	23.65593422578794	26.862550432429472	25.944729455352487	23.5367858864301
32-33	23.4073847036982	26.931962394865174	25.93882423021619	23.721828671220443
34-35	23.522839552267072	26.96920997410266	25.99824898028534	23.509701493344934
36-37	23.69832148306873	26.816813306927262	25.937989026330317	23.54687618367369
38-39	23.538526897528453	26.8946716994591	25.97384468651346	23.592956716498986
40-41	23.52577544514612	26.87919800106319	26.04124279013068	23.553783763660007
42-43	23.45821959329093	26.845997371309178	26.102947462326465	23.59283557307343
44-45	23.411472135553225	26.84971072621106	26.011138117548626	23.727679020687084
46-47	23.308103578972332	26.933384922435653	26.027675908776445	23.730835589815566
48-49	23.228377979500877	26.900942551042224	26.002796751232832	23.867882718224067
50-51	23.149718693964395	27.009664254894993	25.962973815318335	23.877643235822273
52-53	23.21872022723505	26.890231048559716	25.88289707372543	24.008151650479807
54-55	23.16880855786829	26.763142675580536	25.901581224773906	24.166467541777266
56-57	23.20627438688878	26.7842879710325	25.79357365841537	24.215863983663354
58-59	23.165905025619097	26.560895289972525	25.972125625546127	24.301074058862252
60-61	23.303584028008036	26.54978326495524	26.020953218647435	24.125679488389288
62-63	23.17595851243891	26.48206346938864	26.12328956880468	24.21868844936777
64-65	23.152213504440684	26.61646217165029	25.976173064399642	24.25515125950938
66-67	23.139440820525785	26.633587558284834	25.97333992376281	24.253631697426574
68-69	23.384186195493147	26.498446062347963	25.987356401443996	24.130011340714898
70-71	23.209870611517704	26.45352108774281	26.014131791683333	24.322476509056155
72-73	23.292617298338993	26.450845498410963	26.019283946252152	24.23725325699789
74-75	23.20946358365288	26.53276964754631	26.106585444196924	24.15118132460388
76-77	23.125872183821663	26.34936185419976	26.224423246633183	24.30034271534539
78-79	23.178274794401194	26.407367947903065	26.1953903363746	24.21896692132114
80-81	23.26373873259648	26.339329195837568	26.20043868363741	24.196493387928538
82-83	23.210068732702645	26.224900572000504	26.262628449027105	24.302402246269743
84-85	23.31354237760312	26.20014732280947	26.169886187393487	24.316424112193918
86-87	23.21185627410232	26.06764427195359	26.31424597751799	24.406253476426098
88-89	23.244567095534336	26.029621060499142	26.23145005051676	24.494361793449766
90-91	23.34754135191393	26.107921347329672	26.17034341443945	24.37419388631695
92-93	23.38626262101678	26.083016320224356	26.105088222692512	24.425632836066356
94-95	23.242792207792206	26.158116883116882	26.143961038961038	24.45512987012987
96-97	23.239091563472954	26.108958191125907	26.16563916618585	24.486311079215287
98-99	23.177075499648005	26.340183729970747	25.940058470356774	24.542682300024477
100-101	23.17958945403755	26.52354101466155	25.912940637132596	24.3839288941683
102-103	23.039813522073278	26.761776699087953	25.833974574811457	24.364435204027313
104-105	22.956550078435047	26.91082771534689	25.743574850666118	24.389047355551945
106-107	22.898715724608586	26.98426337872542	25.872559449277777	24.244461447388215
108-109	22.958948219672436	27.21748707310062	25.729681477043282	24.09388323018366
110-111	22.995977810970754	27.51813210086922	25.5235090163223	23.96238107183773
112-113	23.018744524586985	27.67961951722872	25.368692237638985	23.932943720545307
114-115	23.10594065685125	27.835712945272206	25.262861928250818	23.795484469625723
116-117	23.05594213375293	27.829517932779403	25.31821457867546	23.79632535479221
118-119	23.244994114542113	27.904233785666317	25.17233819643773	23.678433903353834
120-121	23.32584649123282	28.06533144700993	25.117752691745242	23.491069370012006
122-123	23.523869591453856	28.18634220079356	24.898575555799894	23.391212651952692
124-125	23.69394567867024	28.387462709831617	24.642425711922897	23.27616589957525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2284.0
1	1659.5
2	952.0
3	800.0
4	647.0
5	500.0
6	466.0
7	506.5
8	550.0
9	589.0
10	598.5
11	590.0
12	620.0
13	698.0
14	760.0
15	886.5
16	1056.5
17	1257.5
18	1494.0
19	1776.5
20	2230.5
21	2810.0
22	3517.0
23	4388.0
24	5384.5
25	6755.5
26	8717.5
27	10980.5
28	12709.0
29	14693.5
30	17155.0
31	19873.5
32	23470.0
33	26915.5
34	29943.5
35	33372.0
36	38077.5
37	42815.0
38	47286.5
39	51462.5
40	55666.0
41	60327.5
42	64519.5
43	69880.5
44	76368.5
45	82712.5
46	88600.0
47	93328.0
48	97181.5
49	100912.0
50	104833.5
51	105269.0
52	101029.0
53	97246.0
54	94280.0
55	89550.5
56	81632.5
57	73107.0
58	66386.0
59	59654.0
60	50984.0
61	41442.5
62	33724.0
63	25875.5
64	18695.0
65	14274.5
66	10897.0
67	8098.0
68	5932.5
69	4451.5
70	3500.5
71	2745.5
72	2229.0
73	1793.0
74	1294.0
75	941.0
76	670.0
77	460.0
78	352.0
79	241.5
80	150.0
81	111.0
82	100.0
83	81.5
84	55.5
85	44.0
86	33.0
87	28.0
88	28.0
89	24.5
90	14.5
91	10.0
92	13.0
93	11.5
94	11.0
95	11.5
96	8.5
97	6.0
98	7.5
99	10.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28391236779194984
2	0.06604725557865744
3	0.07551965176184405
4	0.07811482879833355
5	0.08689517777178965
6	0.11894561417243482
7	0.10679153505154237
8	0.10692129390336685
9	0.113798513050064
10-11	0.09215041127068085
12-13	0.08269964156279831
14-15	0.0910690875054769
16-17	0.0925180613508502
18-19	0.08607337171023464
20-21	0.08564084220415306
22-23	0.06797201188072047
24-25	0.07973681444613948
26-27	0.09096095512895651
28-29	0.08531644507459188
30-31	0.0793907908412742
32-33	0.08782511620986505
34-35	0.08211572672958818
36-37	0.08244012385914935
38-39	0.0720810421884955
40-41	0.08447301253773279
42-43	0.07376790726221366
44-45	0.07035092416416917
46-47	0.06122455158584781
48-49	0.062414007727572156
50-51	0.06522544951710244
52-53	0.07673073437887248
54-55	0.08101277648908015
56-57	0.09005264316618519
58-59	0.060554130851421355
60-61	0.07729302273677854
62-63	0.081791329600027
64-65	0.07337863070674024
66-67	0.06747460294872665
68-69	0.07443832799664012
70-71	0.07478435160150539
72-73	0.056336968167125946
74-75	0.08421349483408384
76-77	0.08851716341959558
78-79	0.06901008269531626
80-81	0.08166157074820252
82-83	0.08728445432726306
84-85	0.09029053439453005
86-87	0.07690374618130512
88-89	0.05888889225300727
90-91	0.08220223263080449
92-93	0.058434736271621615
94-95	0.08568409515476122
96-97	0.07275146292292195
98-99	0.06831803548558574
100-101	0.08475415671668582
102-103	0.05878075987648687
104-105	0.0636034638692965
106-107	0.08319705049479213
108-109	0.08949035480827913
110-111	0.08306729164296765
112-113	0.07149712735528535
114-115	0.08544620392641634
116-117	0.07897988781049671
118-119	0.070307671213561
120-121	0.10266087826846329
122-123	0.08161831779759436
124-125	0.10341780490410604
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2311981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.18759373564386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.71647782348992	32.63094122567069
2	14.493678857228181	13.96831017014005
3	6.586401038200888	9.521484522265421
4	3.6377163864048767	7.011711974142906
5	2.0780635188245222	5.006844030098929
6	1.3644217845795206	3.9448921583628067
7	0.8929006609804968	3.0118714008331238
8	0.6362164493192226	2.452619183018284
9	0.48923580400445876	2.121758654786815
>10	2.0191813777270546	15.853144937730764
>50	0.0638614647500337	2.079779484432272
>100	0.02067314160310154	1.705862894717668
>500	6.309115549401775E-4	0.20265177689365255
>1k	5.407813328054435E-4	0.48812758690663777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2723	0.11777778450601455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.3252950608158113E-5
6	0.0	0.0	0.0	0.0	1.7301180243263245E-4
7	0.0	0.0	0.0	0.0	1.7301180243263245E-4
8	0.0	0.0	0.0	0.0	1.7301180243263245E-4
9	0.0	0.0	0.0	0.0	1.7301180243263245E-4
10-11	0.0	0.0	0.0	4.3252950608158113E-5	1.7301180243263245E-4
12-13	0.0	0.0	0.0	4.3252950608158113E-5	3.027706542571068E-4
14-15	0.0	0.0	0.0	4.3252950608158113E-5	3.6765008016934395E-4
16-17	0.0	0.0	0.0	8.650590121631623E-5	3.89276555473423E-4
18-19	0.0	0.0	0.0	1.2975885182447435E-4	4.325295060815811E-4
20-21	0.0	0.0	0.0	2.8114417895302773E-4	5.190354072978974E-4
22-23	0.0	0.0	0.0	5.839148332101345E-4	5.190354072978974E-4
24-25	0.0	0.0	0.0	0.0011029502405080318	5.190354072978974E-4
26-27	0.0	0.0	0.0	0.0021410210551038268	6.271677838182926E-4
28-29	0.0	0.0	0.0	0.007288122177474642	6.487942591223716E-4
30-31	0.0	0.0	0.0	0.022815931445803406	6.487942591223716E-4
32-33	0.0	0.0	0.0	0.06416575222720255	6.487942591223716E-4
34-35	0.0	0.0	0.0	0.13086180206498238	8.650590121631622E-4
36-37	0.0	0.0	0.0	0.24807729821309085	8.650590121631622E-4
38-39	0.0	0.0	0.0	0.43955811055540683	9.083119627713203E-4
40-41	0.0	0.0	0.0	0.6629379739712393	9.515649133794785E-4
42-43	0.0	0.0	0.0	0.8648860003607296	9.948178639876367E-4
44-45	0.0	0.0	0.0	1.109849086130033	0.0010380708145957948
46-47	0.0	0.0	0.0	1.4170747942997801	0.0010380708145957948
48-49	0.0	0.0	0.0	1.7366059669175482	0.0010380708145957948
50-51	0.0	0.0	0.0	2.086868360942413	0.0010380708145957948
52-53	0.0	0.0	0.0	2.431399739011696	0.001124576715812111
54-55	0.0	0.0	0.0	2.8111822718266284	0.0012543355676365852
56-57	0.0	0.0	0.0	3.268128068526515	0.0012543355676365852
58-59	0.0	0.0	0.0	3.7632445941381008	0.0012543355676365852
60-61	0.0	0.0	0.0	4.29808895488328	0.0012975885182447433
62-63	0.0	0.0	0.0	4.827742961555479	0.0015138532712855339
64-65	0.0	0.0	0.0	5.375195557402938	0.0015138532712855339
66-67	2.1626475304079057E-5	0.0	0.0	6.019469883186757	0.0015138532712855339
68-69	4.3252950608158113E-5	0.0	0.0	6.7517855899334815	0.0015138532712855339
70-71	4.3252950608158113E-5	0.0	0.0	7.566130517508578	0.0015354797465896128
72-73	4.3252950608158113E-5	0.0	0.0	8.366656127364369	0.001578732697197771
74-75	4.3252950608158113E-5	0.0	0.0	9.167419628448503	0.0016003591725018502
76-77	4.3252950608158113E-5	0.0	0.0	10.03801501828951	0.0016003591725018502
78-79	4.3252950608158113E-5	0.0	0.0	11.017975493743243	0.0016436121231100083
80-81	4.3252950608158113E-5	0.0	0.0	12.057754799888063	0.0017084915490222456
82-83	4.3252950608158113E-5	0.0	0.0	13.13179044291454	0.0017733709749344826
84-85	4.3252950608158113E-5	0.0	0.0	14.1627894000859	0.0017949974502385617
86-87	4.3252950608158113E-5	0.0	0.0	15.250492975504557	0.001924756302063036
88-89	4.3252950608158113E-5	0.0	0.0	16.43108226235423	0.0020328886785834313
90-91	4.3252950608158113E-5	0.0	0.0	17.672550077184887	0.0020328886785834313
92-93	4.3252950608158113E-5	0.0	0.0	18.929805218987525	0.00205451515388751
94-95	4.3252950608158113E-5	0.0	0.0	20.149538426137585	0.0020761416291915896
96-97	4.3252950608158113E-5	0.0	0.0	21.370828739509538	0.002335659332840538
98-99	4.3252950608158113E-5	0.0	0.0	22.670342014056345	0.002422165234056854
100-101	4.3252950608158113E-5	0.0	0.0	24.063043770688424	0.002422165234056854
102-103	4.3252950608158113E-5	0.0	0.0	25.46904580963252	0.002422165234056854
104-105	4.3252950608158113E-5	0.0	0.0	26.816007570996476	0.002422165234056854
106-107	4.3252950608158113E-5	0.0	0.0	28.122635955918323	0.002422165234056854
108-109	4.3252950608158113E-5	0.0	0.0	29.469554464331672	0.002422165234056854
110-111	4.3252950608158113E-5	0.0	0.0	30.84458739064032	0.002422165234056854
112-113	4.3252950608158113E-5	2.1626475304079057E-5	0.0	32.25683948094729	0.002422165234056854
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7140	0.0	28.07942	1
GTACATG	10870	0.0	27.995466	1
TACATGG	10530	0.0	27.420252	2
CATGGGG	6320	0.0	25.904411	4
ACATGGG	11290	0.0	24.888927	3
AACGCAG	8060	0.0	24.297028	6
TATCAAC	8410	0.0	23.572538	2
TCAACGC	8385	0.0	23.358822	4
TTACGTA	205	2.2538588E-6	23.232407	4
ATCAACG	8645	0.0	22.6563	3
CAACGCA	8655	0.0	22.629635	5
GGTATCA	5515	0.0	21.530525	1
ACGCAGA	9040	0.0	21.529501	7
CGCAGAG	9340	0.0	20.710077	8
ATGGGGA	3100	0.0	20.163982	5
GCGTAGA	730	0.0	19.617128	1
GCAGAGT	10005	0.0	18.73826	9
ATGGGGG	2775	0.0	18.66401	5
GTATAAA	1205	0.0	18.321531	1
GTATAAT	1085	0.0	18.148106	1
>>END_MODULE