FastQCFastQC Report
Mon 15 Aug 2016
SRR2921100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311981
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71320.3084800437373837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.2538948200698881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.18702575842967567No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT23400.10121190442308999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.044.8581541
GTATCAA95400.043.5172921
TCAACGC121400.033.868684
TATCAAC122200.033.7444842
ATCAACG121500.033.7427183
CAACGCA123450.033.257995
AACGCAG125550.032.759726
CATGGGG65600.030.7029974
TACATGG105050.029.5535452
GTACATG108800.029.2212831
ACGCAGA141750.028.8309427
CGCAGAG144150.028.2271218
ACATGGG111400.027.7619553
TACCGTA2256.5720087E-926.4388567
TACACCG2506.89397E-1026.2192675
GCAGAGT154150.025.7399379
AGTACTT90950.025.6402712-13
GAGTACT90950.024.5283212-13
AGAGTAC142400.023.4774810-11
GTACTTT98450.022.78181814-15
Produced by FastQC (version 0.11.5)