FastQCFastQC Report
Mon 15 Aug 2016
SRR2921099_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921099_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834368
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30870.10891316864994242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG145050.032.1610641
TACATGG143950.031.1361262
CATGGGG92850.029.6816524
GTATCAA96650.029.0093121
ACATGGG155800.027.8897323
AACGCAG104500.026.0827736
ATGGGGA44500.024.478235
TCAACGC111950.024.298594
TATCAAC114050.024.1638982
CAACGCA115650.023.7785455
ATCAACG116250.023.3998033
GGTATCA74400.022.7712481
ACGCAGA119150.022.724747
GTACTAG3204.8748916E-1022.3703441
CGCAGAG119500.022.2099978
GTCTTAG14450.020.641611
ATGGGGC39400.020.5462065
GCAGAGT127500.020.5363889
GTACAAA20750.018.3993851
GAGTACT36600.018.28939412-13
Produced by FastQC (version 0.11.5)