FastQCFastQC Report
Mon 15 Aug 2016
SRR2921099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834368
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94760.33432497121051324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78290.2762167791902816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.18684941404926955No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT29560.10429132702598957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121900.043.1226351
GGTATCA79100.043.0717661
TATCAAC156000.033.508492
TCAACGC156100.033.372474
ATCAACG156650.033.2553023
CAACGCA158600.032.8840075
AACGCAG160500.032.785496
GTACATG136600.028.8724631
TACATGG136800.028.3644772
ACGCAGA186300.027.9069127
CATGGGG88600.027.8513664
CGCAGAG189050.027.4380328
ACATGGG143850.026.8086093
GCAGAGT199050.025.5818779
AGTACTT123400.024.15188412-13
GAGTACT119600.023.62603812-13
AGAGTAC187750.022.74875510-11
TAGACAG10500.022.1388555
CAGAGTA193950.021.80685210-11
GTACTTT130800.021.71748214-15
Produced by FastQC (version 0.11.5)