##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2834368
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40922808894258	33.0	31.0	34.0	28.0	34.0
2	31.472140526565358	33.0	31.0	34.0	28.0	34.0
3	31.56464897994897	34.0	31.0	34.0	28.0	34.0
4	35.05131514327003	37.0	35.0	37.0	32.0	37.0
5	34.829246943233905	37.0	35.0	37.0	32.0	37.0
6	34.58200734696412	37.0	35.0	37.0	31.0	37.0
7	34.530584243118746	37.0	35.0	37.0	31.0	37.0
8	34.52424667509653	37.0	35.0	37.0	31.0	37.0
9	36.09281822261612	39.0	37.0	39.0	31.0	39.0
10-11	36.066184595648835	39.0	37.0	39.0	31.0	39.0
12-13	36.006856025752484	39.0	37.0	39.0	30.0	39.0
14-15	37.278791603630864	40.0	38.0	41.0	31.0	41.0
16-17	37.27424244134848	40.0	38.0	41.0	31.0	41.0
18-19	37.30139382042134	40.0	38.0	41.0	31.0	41.0
20-21	37.30735634892858	40.0	38.0	41.0	31.0	41.0
22-23	37.25703296113984	40.0	37.5	41.0	31.0	41.0
24-25	37.20301104161492	40.0	37.0	41.0	31.0	41.0
26-27	37.06855743502608	40.0	37.0	41.0	30.0	41.0
28-29	36.93554824214781	40.0	37.0	41.0	30.0	41.0
30-31	36.77247732122294	40.0	37.0	41.0	30.0	41.0
32-33	36.60747969212184	40.0	36.0	41.0	30.0	41.0
34-35	36.43544716141305	40.0	36.0	41.0	30.0	41.0
36-37	36.233014202813465	40.0	35.5	41.0	29.0	41.0
38-39	36.07078544493869	40.0	35.0	41.0	28.0	41.0
40-41	35.90128892931334	40.0	35.0	41.0	27.5	41.0
42-43	35.70953824626866	40.0	35.0	41.0	27.0	41.0
44-45	35.4851095552871	40.0	35.0	41.0	26.0	41.0
46-47	35.13463989150315	39.5	35.0	41.0	24.0	41.0
48-49	34.81591681108452	39.0	35.0	41.0	23.0	41.0
50-51	34.579235476832935	39.0	34.5	41.0	22.0	41.0
52-53	34.34572521987265	39.0	34.0	41.0	20.5	41.0
54-55	34.11028878395466	38.5	34.0	41.0	19.5	41.0
56-57	33.84323595242397	38.0	34.0	40.5	18.0	41.0
58-59	33.572472417131436	37.5	34.0	40.0	17.5	41.0
60-61	33.26141083303227	37.0	33.0	40.0	14.0	41.0
62-63	32.94587029630591	36.5	33.0	40.0	12.5	41.0
64-65	32.6138283737327	36.0	33.0	39.0	9.0	41.0
66-67	32.25386082541152	35.5	32.5	39.0	7.5	41.0
68-69	31.889697103551832	35.0	32.0	38.5	6.0	40.5
70-71	31.496919242667147	35.0	32.0	37.5	2.0	40.0
72-73	31.123033776841964	35.0	31.5	37.0	2.0	39.0
74-75	30.816892160792108	35.0	31.0	36.5	2.0	39.0
76-77	30.53421274160589	35.0	31.0	36.0	2.0	38.5
78-79	30.263733749463725	35.0	31.0	35.5	2.0	37.0
80-81	30.24381502331384	35.0	31.0	35.0	2.0	37.0
82-83	30.079830142028136	35.0	31.0	35.0	2.0	36.5
84-85	29.90840656541423	35.0	31.0	35.0	2.0	36.0
86-87	29.763423274606545	35.0	31.0	35.0	2.0	36.0
88-89	29.61516306280624	35.0	31.0	35.0	2.0	35.5
90-91	29.479405991035744	35.0	31.0	35.0	2.0	35.0
92-93	29.38335247928286	35.0	31.0	35.0	2.0	35.0
94-95	29.285786108225892	35.0	31.0	35.0	2.0	35.0
96-97	29.227910419536208	35.0	31.0	35.0	2.0	35.0
98-99	29.138916153442317	35.0	30.5	35.0	2.0	35.0
100-101	29.044216382629216	35.0	30.0	35.0	2.0	35.0
102-103	27.70939888539526	32.5	28.0	34.5	2.0	35.0
104-105	28.72675037256983	34.0	29.5	35.0	2.0	35.0
106-107	28.889319947162825	34.0	30.0	35.0	2.0	35.0
108-109	28.880426077347753	35.0	30.0	35.0	2.0	35.0
110-111	28.773421623444804	34.5	30.0	35.0	2.0	35.0
112-113	28.636489510183576	34.0	30.0	35.0	2.0	35.0
114-115	28.51758487253596	34.0	29.5	35.0	2.0	35.0
116-117	28.37225564923115	34.0	29.0	35.0	2.0	35.0
118-119	28.19107628226116	34.0	29.0	35.0	2.0	35.0
120-121	28.290730243920336	35.0	30.0	35.0	2.0	35.0
122-123	28.160686438740488	35.0	30.0	35.0	2.0	35.0
124-125	27.445030426536004	34.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43356.0
3	17840.0
4	8311.0
5	8017.0
6	10158.0
7	11970.0
8	12461.0
9	13721.0
10	16220.0
11	19169.0
12	20551.0
13	24546.0
14	32196.0
15	29346.0
16	15717.0
17	13150.0
18	11951.0
19	11662.0
20	11727.0
21	12279.0
22	12711.0
23	13317.0
24	16943.0
25	21778.0
26	27013.0
27	32058.0
28	38773.0
29	46431.0
30	56297.0
31	69109.0
32	87735.0
33	113372.0
34	156205.0
35	243456.0
36	481998.0
37	816247.0
38	256181.0
39	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.522249069561234	17.39113839903392	11.699797036944364	25.386815494460485
2	17.295537787312725	20.38327181882979	35.92928066844976	26.391909725407725
3	19.712754307133018	23.841787657777676	28.980428794002755	27.465029241086548
4	13.536055266630441	15.700182763754913	34.192593481190855	36.57116848842379
5	14.148736212098727	37.090743999039	33.381206763756104	15.37931302510617
6	34.1085783146313	34.72586727810628	16.040191750437728	15.125362656824691
7	29.02267794759864	31.870008887985623	20.269335249948575	18.837977914467164
8	27.968987018174413	32.90222893898073	19.380820094571742	19.747963948273117
9	27.244855040655803	14.071169173574367	18.584767448261065	40.09920833750877
10-11	26.1375263740994	24.879896408888513	26.701102948959505	22.281474268052587
12-13	26.842870939138642	22.528216218428657	27.048985973310018	23.579926869122687
14-15	24.056320743415746	23.55742402181187	24.91549647880677	27.47075875596562
16-17	23.683511299916475	26.406168896586014	25.717979688485258	24.19234011501225
18-19	23.646539365789323	24.923500377138772	28.193836096812184	23.23612416025972
20-21	24.444344850439016	24.107315207145923	28.002591639914503	23.445748302500558
22-23	24.195637593899257	24.173969721795462	27.769248454796635	23.861144229508643
24-25	23.733137734702478	24.363833921376127	28.06132207641333	23.841706267508066
26-27	23.599599119191463	24.40997120433629	28.12487648918751	23.86555318728474
28-29	23.69184905283525	24.429057172181732	28.17189430656615	23.707199468416874
30-31	23.79734734362944	24.512148406752875	27.904414171379692	23.786090078237994
32-33	23.49453149605952	24.553825306067715	27.967826828797055	23.983816369075715
34-35	23.70241213258154	24.468620820832264	28.000185619237776	23.82878142734842
36-37	23.98203139019229	24.409896604092253	27.776214265385125	23.831857740330335
38-39	23.92331336458299	24.43099824284642	27.752987152259063	23.892701240311528
40-41	23.770668543160173	24.516260630267492	27.745924520071963	23.96714630650037
42-43	23.99761660345878	24.417216493499296	27.52018423507051	24.064982667971407
44-45	23.809739765760423	24.508989253975653	27.445498296874167	24.235772683389765
46-47	23.99017097395301	24.52829356363246	27.16491250854023	24.316622953874298
48-49	23.99542534273319	24.81458681906585	26.628270457379273	24.56171738082169
50-51	23.732937846708	25.166506291335462	26.46902215508453	24.631533706872002
52-53	23.869257143179762	24.927869970636333	26.479311786098847	24.72356110008506
54-55	23.562222642532095	25.092628925349203	26.36870264456464	24.97644578755406
56-57	23.526834839198447	24.971766683151174	26.252988514893126	25.24840996275725
58-59	23.391455156863643	24.662190057127937	26.462937413328913	25.483417372679508
60-61	23.441740489746515	24.88380914741621	26.481475273605593	25.192975089231684
62-63	23.121535361804384	24.908380057798368	26.593389532073154	25.376695048324095
64-65	23.058136535226385	24.781827864134577	26.61604774325192	25.543987857387112
66-67	23.078827264320743	24.84510833462281	26.562762729170412	25.513301671886033
68-69	23.260015579250837	24.606853282449347	26.48837863754776	25.644752500752055
70-71	23.097992177056557	24.5253052881799	26.541738833390504	25.834963701373038
72-73	23.169893530350212	24.54122163145941	26.36041856616684	25.92846627202354
74-75	22.97141893315276	24.647901736769917	26.3903175050698	25.990361825007525
76-77	22.809280669435516	24.395965067468225	26.563441089371093	26.231313173725162
78-79	22.836287983762922	24.39538787307159	26.474528820853298	26.293795322312192
80-81	22.74100562538771	24.42172341102734	26.477378827624314	26.35989213596063
82-83	22.516191159066096	24.357487806788676	26.461032889030662	26.665288145114562
84-85	22.49867369044685	24.34996717527205	26.254436542946586	26.896922591334523
86-87	22.35036602461664	24.24484818631953	26.36834493078753	27.0364408582763
88-89	22.301093907199093	24.156349789169052	26.251902489495503	27.290653814136352
90-91	22.245074393550972	24.170237992318047	26.158300443026693	27.42638717110428
92-93	22.22310045346137	24.17030966002218	26.103351506838973	27.50323837967748
94-95	22.016787182518975	23.933061568410345	26.301248347339705	27.748902901730975
96-97	22.005854267898922	23.82743495551956	26.2694657054234	27.897245071158117
98-99	21.933488697404947	23.88450878581002	26.123854619316855	28.05814789746818
100-101	21.94635930411822	23.944055884826916	26.06865756391971	28.04092724713515
102-103	21.787787761295416	23.914797513971777	26.026174754198152	28.271239970534655
104-105	21.60471610432338	23.946219417268814	26.041166055170827	28.407898423236983
106-107	21.399395212620398	24.042741978027717	26.113271570141166	28.444591239210716
108-109	21.24480745094843	24.165443178085248	26.19798673939499	28.391762631571332
110-111	21.2654270994671	24.30523574750038	25.981118179530803	28.44821897350172
112-113	21.193721962650624	24.456402263559994	25.859570922915527	28.490304850873855
114-115	20.992270589868795	24.62086441449158	25.834800464565582	28.552064531074045
116-117	20.70057154228912	24.55601206173701	26.056213537627297	28.687202858346573
118-119	20.731319414612802	24.5544365811426	26.080073954045112	28.63417005019948
120-121	20.66640604905703	24.661176526209534	26.068070591149183	28.60434683358425
122-123	20.60055256113905	24.911436209108103	25.82024597985398	28.66776524989887
124-125	20.404853657310344	25.082262911435837	25.79591671128289	28.716966719970927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	225.0
1	171.5
2	297.5
3	551.5
4	420.0
5	266.0
6	507.0
7	1039.5
8	1627.5
9	1788.0
10	1434.0
11	1110.0
12	1153.0
13	1308.5
14	1446.0
15	1668.5
16	1875.0
17	2051.0
18	2346.5
19	2666.0
20	2999.0
21	3459.5
22	4080.0
23	4836.0
24	5724.5
25	6883.0
26	8450.0
27	10428.0
28	12235.5
29	14367.5
30	16858.0
31	19637.5
32	23461.0
33	26416.5
34	27940.5
35	30065.5
36	34072.5
37	38547.0
38	43620.0
39	49368.0
40	54475.0
41	59560.5
42	65174.0
43	73473.5
44	82094.0
45	90433.5
46	100388.5
47	109085.5
48	116355.5
49	123540.5
50	132598.0
51	137358.0
52	135434.0
53	134753.0
54	132537.0
55	127302.5
56	119974.0
57	109955.0
58	100303.5
59	89711.0
60	76486.5
61	62755.5
62	50271.0
63	38037.0
64	27648.5
65	20608.5
66	15015.0
67	10800.5
68	7760.0
69	5517.0
70	4246.5
71	3363.0
72	2712.5
73	2264.5
74	1723.5
75	1226.5
76	807.5
77	485.0
78	302.0
79	170.5
80	101.0
81	66.0
82	41.0
83	28.5
84	23.5
85	11.5
86	10.0
87	9.0
88	7.0
89	6.5
90	4.5
91	5.0
92	5.5
93	4.0
94	5.5
95	13.5
96	13.5
97	7.5
98	6.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16896182852755887
2	0.012277869352180098
3	0.0
4	0.003810373247228306
5	0.14070156027728228
6	0.07567824643800664
7	0.007232652922979655
8	0.011607525910538081
9	0.011713369611849978
10-11	0.005221622598053605
12-13	0.009561214351841398
14-15	0.011095948020863911
16-17	0.014430024612188678
18-19	0.018716694515320525
20-21	0.02261527084697541
22-23	0.02397359834714476
24-25	0.023779551561406282
26-27	0.02145099013254454
28-29	0.020057381398604558
30-31	0.02266819269763136
32-33	0.022597630230090093
34-35	0.021521552600085803
36-37	0.022879880100255153
38-39	0.018469725878926094
40-41	0.02519080091223158
42-43	0.021062896561067582
44-45	0.031806032244225166
46-47	0.025402488314855375
48-49	0.017446570099577754
50-51	0.013794962404317294
52-53	0.014941602501862848
54-55	0.016934992209903584
56-57	0.013883165488743875
58-59	0.013336306365299073
60-61	0.015982398898096505
62-63	0.013336306365299073
64-65	0.02508495721091968
66-67	0.01878725698286179
68-69	0.016599820489082574
70-71	0.02300336441845237
72-73	0.02954803328290469
74-75	0.01513564928760133
76-77	0.007726590195768509
78-79	0.01273652539119832
80-81	0.015170930521371959
82-83	0.01460643078104184
84-85	0.013283384514643122
86-87	0.009490651884300134
88-89	0.020498396820737463
90-91	0.015982398898096505
92-93	0.013724399936776028
94-95	0.010619651364960372
96-97	0.018981303768600268
98-99	0.020957052859755687
100-101	0.026619690879942197
102-103	0.018716694515320525
104-105	0.011783932079391244
106-107	0.016917351593018267
108-109	0.009137839546593808
110-111	0.02247414591189288
112-113	0.0149945243525188
114-115	0.01137819789102897
116-117	0.009949307923318355
118-119	0.006191856526745998
120-121	0.002646092532797435
122-123	0.0039338575654255204
124-125	3.704529545916409E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2834368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.251493066649935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.14950512139404	23.896648452586344
2	13.90002541274456	10.355934005782236
3	6.5873332191068865	7.361639932178187
4	3.9858358676537406	5.939133487548317
5	2.5392725852043623	4.729584755103727
6	1.767879119827009	3.9513682064946605
7	1.2955749647719237	3.3783471272268577
8	0.9871067850733342	2.9416961248161932
9	0.7318797328635351	2.4537251514948824
>10	3.812476136930311	24.697742662974985
>50	0.17985987878113444	4.540332206859643
>100	0.059642841268798465	3.7017424330685196
>500	0.0022789480297461945	0.5858054680205976
>1k	0.001044517846950992	0.6668440723987883
>5k	2.848685037139069E-4	0.7994559134460542
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9476	0.33432497121051324	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7829	0.2762167791902816	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5296	0.18684941404926955	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	2956	0.10429132702598957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.5281233770632467E-5	0.0
8	0.0	0.0	0.0	3.5281233770632467E-5	0.0
9	0.0	0.0	0.0	7.056246754126493E-5	0.0
10-11	0.0	0.0	0.0	7.056246754126493E-5	0.0
12-13	0.0	1.7640616885316233E-5	0.0	7.056246754126493E-5	0.0
14-15	0.0	3.5281233770632467E-5	0.0	7.056246754126493E-5	0.0
16-17	0.0	3.5281233770632467E-5	0.0	1.058437013118974E-4	0.0
18-19	0.0	3.5281233770632467E-5	0.0	1.7640616885316232E-4	0.0
20-21	0.0	3.5281233770632467E-5	0.0	2.1168740262379477E-4	0.0
22-23	0.0	3.5281233770632467E-5	0.0	4.939372727888545E-4	0.0
24-25	0.0	3.5281233770632467E-5	0.0	0.0013759681170546663	0.0
26-27	0.0	3.5281233770632467E-5	0.0	0.0026813737665680676	0.0
28-29	0.0	3.5281233770632467E-5	0.0	0.009084917695937859	0.0
30-31	0.0	3.5281233770632467E-5	0.0	0.03016545487389076	0.0
32-33	0.0	3.5281233770632467E-5	0.0	0.07917108858129926	0.0
34-35	0.0	3.5281233770632467E-5	0.0	0.15950645787702938	0.0
36-37	0.0	3.5281233770632467E-5	0.0	0.2989378937385689	0.0
38-39	0.0	3.5281233770632467E-5	0.0	0.5183342459412469	0.0
40-41	0.0	3.5281233770632467E-5	0.0	0.7742819563303001	0.0
42-43	0.0	3.5281233770632467E-5	0.0	1.0060443809695847	0.0
44-45	0.0	3.5281233770632467E-5	0.0	1.28190834782216	0.0
46-47	0.0	3.5281233770632467E-5	0.0	1.6252300336441845	0.0
48-49	0.0	3.5281233770632467E-5	0.0	1.9875153826179242	0.0
50-51	0.0	3.5281233770632467E-5	0.0	2.3875516517262403	0.0
52-53	0.0	3.5281233770632467E-5	0.0	2.769594491611534	0.0
54-55	0.0	3.5281233770632467E-5	0.0	3.194680436696999	0.0
56-57	0.0	3.5281233770632467E-5	0.0	3.7047235927021474	0.0
58-59	0.0	3.5281233770632467E-5	0.0	4.2712520039740784	0.0
60-61	0.0	3.5281233770632467E-5	0.0	4.876819100413214	0.0
62-63	0.0	3.5281233770632467E-5	0.0	5.472719138799196	0.0
64-65	0.0	3.5281233770632467E-5	0.0	6.09712641407185	0.0
66-67	0.0	3.5281233770632467E-5	0.0	6.819686081694403	0.0
68-69	0.0	3.5281233770632467E-5	0.0	7.645125826992119	0.0
70-71	0.0	3.5281233770632467E-5	0.0	8.541163321064872	0.0
72-73	0.0	3.5281233770632467E-5	0.0	9.423829227538555	0.0
74-75	0.0	3.5281233770632467E-5	0.0	10.287372705308556	0.0
76-77	0.0	3.5281233770632467E-5	0.0	11.24785137286337	0.0
78-79	0.0	3.5281233770632467E-5	0.0	12.326522173549801	0.0
80-81	0.0	3.5281233770632467E-5	0.0	13.460178777067764	0.0
82-83	0.0	3.5281233770632467E-5	0.0	14.616380088965158	0.0
84-85	0.0	3.5281233770632467E-5	0.0	15.719377300336442	0.0
86-87	0.0	3.5281233770632467E-5	0.0	16.868769334116106	0.0
88-89	0.0	3.5281233770632467E-5	0.0	18.14212198274889	0.0
90-91	0.0	3.5281233770632467E-5	0.0	19.490288487592295	0.0
92-93	0.0	3.5281233770632467E-5	0.0	20.816580627497913	0.0
94-95	0.0	3.5281233770632467E-5	0.0	22.093831852462348	0.0
96-97	0.0	3.5281233770632467E-5	0.0	23.38311397814257	0.0
98-99	0.0	3.5281233770632467E-5	0.0	24.749203349741457	0.0
100-101	0.0	3.5281233770632467E-5	0.0	26.225511295639805	0.0
102-103	0.0	3.5281233770632467E-5	0.0	27.69299893309549	0.0
104-105	0.0	3.5281233770632467E-5	0.0	29.088265885022693	0.0
106-107	0.0	3.5281233770632467E-5	0.0	30.4377554361325	0.0
108-109	0.0	3.5281233770632467E-5	0.0	31.819192144421613	0.0
110-111	0.0	3.5281233770632467E-5	0.0	33.260042450380475	0.0
112-113	0.0	3.5281233770632467E-5	0.0	34.73850255153883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12190	0.0	43.122635	1
GGTATCA	7910	0.0	43.071766	1
TATCAAC	15600	0.0	33.50849	2
TCAACGC	15610	0.0	33.37247	4
ATCAACG	15665	0.0	33.255302	3
CAACGCA	15860	0.0	32.884007	5
AACGCAG	16050	0.0	32.78549	6
GTACATG	13660	0.0	28.872463	1
TACATGG	13680	0.0	28.364477	2
ACGCAGA	18630	0.0	27.906912	7
CATGGGG	8860	0.0	27.851366	4
CGCAGAG	18905	0.0	27.438032	8
ACATGGG	14385	0.0	26.808609	3
GCAGAGT	19905	0.0	25.581877	9
AGTACTT	12340	0.0	24.151884	12-13
GAGTACT	11960	0.0	23.626038	12-13
AGAGTAC	18775	0.0	22.748755	10-11
TAGACAG	1050	0.0	22.138855	5
CAGAGTA	19395	0.0	21.806852	10-11
GTACTTT	13080	0.0	21.717482	14-15
>>END_MODULE