FastQCFastQC Report
Mon 15 Aug 2016
SRR2921098_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921098_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5654150
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111500.034.2926561
GTACATG220450.032.9034541
TACATGG221700.031.9223372
CATGGGG156050.030.9347884
ACATGGG230050.030.065883
TCAACGC122600.029.810414
GGTATCA79100.029.1845131
CAACGCA125550.029.157125
ATCAACG128500.028.5343273
AACGCAG128250.028.4928346
TATCAAC142550.026.222312
GTATAGT14850.025.30651
ACGCAGA146000.024.7822537
CGCAGAG157050.022.848518
ATGGGGG79300.021.8426825
ATGGGGA74450.020.9470465
GTATAAG20100.019.2901361
GCAGAGT183050.018.9529729
ATGGGGC74250.017.8769975
GAGTACA161850.017.7644731
Produced by FastQC (version 0.11.5)