FastQCFastQC Report
Mon 15 Aug 2016
SRR2921098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5654150
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73590.13015218910004156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60410.10684187720523862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141300.041.246231
GGTATCA95250.041.0211831
TCAACGC172850.032.824574
CAACGCA174650.032.3838925
ATCAACG179000.031.7633863
AACGCAG183100.030.982256
GTACATG214150.029.9170021
TATCAAC193200.029.8293042
TACATGG215600.029.2179662
ACATGGG216600.028.2306163
CATGGGG148150.027.9183184
ACGCAGA208200.027.0009447
CGCAGAG218350.025.7185618
AGAGTAC216100.021.81547510-11
GCAGAGT252100.021.662159
AGTACTT133250.021.00525512-13
GAGTACT122100.020.85276412-13
CAGAGTA232400.019.51748810-11
GTATAAT18400.019.4520451
GTACTTT131800.019.18411314-15
Produced by FastQC (version 0.11.5)