FastQCFastQC Report
Mon 15 Aug 2016
SRR2921097_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921097_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63250.032.3466451
CATGGGG63250.030.9610354
TACATGG104600.030.5572952
GTACATG108800.029.7691611
ACATGGG107750.029.1120953
AACGCAG72050.027.5896196
TCAACGC72450.027.4402584
CAACGCA74000.026.8654965
ATCAACG74350.026.7390273
GGTATCA47300.026.2299731
TATCAAC77350.026.086252
ATGGGGA33800.024.8303325
ACGCAGA82650.023.9787627
CGCAGAG83200.023.319528
ATGGGGC27700.020.6286875
GCAGAGT93550.019.6580339
GTATAGG6200.019.241281
TAGACCA28300.018.7190464
GTAGACC25450.018.4764863
TACGGTA1959.88818E-418.3145715
Produced by FastQC (version 0.11.5)