##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921097_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2782106
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.258054869224967	31.0	31.0	34.0	26.0	34.0
2	30.442106447417892	31.0	31.0	34.0	26.0	34.0
3	30.638178775359385	33.0	31.0	34.0	26.0	34.0
4	34.036302714562275	37.0	35.0	37.0	30.0	37.0
5	34.168037450765716	37.0	35.0	37.0	30.0	37.0
6	34.165077462900406	37.0	35.0	37.0	30.0	37.0
7	34.03522331643726	37.0	35.0	37.0	30.0	37.0
8	34.052766860788196	37.0	35.0	37.0	30.0	37.0
9	35.56779863887285	39.0	37.0	39.0	30.0	39.0
10-11	35.54388384195282	39.0	37.0	39.0	30.0	39.0
12-13	35.482361024346304	39.0	37.0	39.0	30.0	39.0
14-15	36.77104970119758	40.0	37.0	41.0	30.0	41.0
16-17	36.75951293732158	40.0	37.0	41.0	30.0	41.0
18-19	36.791268017825345	40.0	37.0	41.0	30.0	41.0
20-21	36.82812283212789	40.0	37.0	41.0	30.0	41.0
22-23	36.77887506802401	40.0	37.0	41.0	30.0	41.0
24-25	36.7704949056578	40.0	37.0	41.0	30.0	41.0
26-27	36.71879324511719	40.0	37.0	41.0	30.0	41.0
28-29	36.59958463121103	40.0	37.0	41.0	29.5	41.0
30-31	36.50848332162757	40.0	37.0	41.0	29.0	41.0
32-33	36.38963522597629	40.0	36.5	41.0	29.0	41.0
34-35	36.286444873056595	40.0	36.0	41.0	28.5	41.0
36-37	36.18383267927247	40.0	36.0	41.0	28.0	41.0
38-39	36.05739896323145	40.0	36.0	41.0	27.0	41.0
40-41	35.93554397280334	40.0	36.0	41.0	27.0	41.0
42-43	35.77895306648992	40.0	35.0	41.0	27.0	41.0
44-45	35.61930206828927	40.0	35.0	41.0	26.0	41.0
46-47	35.45574000415513	40.0	35.0	41.0	26.0	41.0
48-49	35.253810422751684	39.5	35.0	41.0	25.5	41.0
50-51	35.09855807075647	39.0	35.0	41.0	25.0	41.0
52-53	34.939370929792034	39.0	35.0	41.0	24.0	41.0
54-55	34.723237540194376	39.0	34.0	41.0	24.0	41.0
56-57	34.50102763877437	38.5	34.0	41.0	23.0	41.0
58-59	34.2458414596712	38.0	34.0	40.0	22.5	41.0
60-61	33.965613998891484	38.0	34.0	40.0	22.0	41.0
62-63	33.690694387632966	37.0	33.0	40.0	21.5	41.0
64-65	33.376745170744755	37.0	33.0	40.0	20.5	41.0
66-67	33.03978173369383	36.0	33.0	39.5	19.5	41.0
68-69	32.67266380217001	36.0	32.0	39.0	18.5	41.0
70-71	32.304564419903485	35.0	32.0	39.0	17.5	40.0
72-73	31.932562418541924	35.0	32.0	38.0	16.0	40.0
74-75	31.86204389768039	35.0	32.0	37.0	17.5	39.5
76-77	31.86671895319589	35.0	32.5	37.0	18.0	39.0
78-79	31.654288513809323	35.0	33.0	36.5	18.0	39.0
80-81	31.41058823783134	35.0	33.0	36.0	16.0	38.0
82-83	31.137354040428367	35.0	32.0	36.0	12.5	37.0
84-85	30.861216646669824	35.0	32.0	35.0	8.5	37.0
86-87	30.690800602133777	35.0	32.0	35.0	7.0	36.5
88-89	30.501910782694836	35.0	32.0	35.0	6.5	36.0
90-91	30.346987857400116	35.0	32.0	35.0	2.0	36.0
92-93	30.217511302588758	35.0	32.0	35.0	2.0	36.0
94-95	30.092158781872435	35.0	32.0	35.0	2.0	35.0
96-97	29.98561593267834	35.0	32.0	35.0	2.0	35.0
98-99	29.880390970006175	35.0	31.0	35.0	2.0	35.0
100-101	29.743660737585124	35.0	31.0	35.0	2.0	35.0
102-103	28.488522004553385	33.5	28.5	34.5	2.0	35.0
104-105	29.401205238046288	34.5	30.5	35.0	2.0	35.0
106-107	29.515266851802195	35.0	31.0	35.0	2.0	35.0
108-109	29.442927767669527	35.0	31.0	35.0	2.0	35.0
110-111	29.33476654016777	35.0	31.0	35.0	2.0	35.0
112-113	29.21290292318122	35.0	31.0	35.0	2.0	35.0
114-115	29.06014867873474	35.0	31.0	35.0	2.0	35.0
116-117	28.904295343168087	35.0	30.0	35.0	2.0	35.0
118-119	28.69180038431318	35.0	30.0	35.0	2.0	35.0
120-121	28.471009372036868	35.0	30.0	35.0	2.0	35.0
122-123	28.153745939227335	35.0	29.5	35.0	2.0	35.0
124-125	26.88495783410122	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75503.0
3	11298.0
4	7517.0
5	7909.0
6	11813.0
7	12093.0
8	10475.0
9	11114.0
10	12421.0
11	13396.0
12	14195.0
13	14088.0
14	14534.0
15	12840.0
16	10103.0
17	9239.0
18	8801.0
19	8729.0
20	9138.0
21	9943.0
22	11830.0
23	15366.0
24	20602.0
25	26945.0
26	30024.0
27	32292.0
28	37841.0
29	44954.0
30	53638.0
31	65729.0
32	82980.0
33	108742.0
34	150680.0
35	231114.0
36	437453.0
37	809934.0
38	336038.0
39	795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57331296846967	18.490089792610757	11.642362328991561	25.294234909928015
2	16.211299026149437	21.071163678402705	38.16207198451277	24.55546531093508
3	19.05851728955892	24.883014344772413	29.381026451996313	26.677441913672357
4	12.654796547969077	16.57029525160476	36.89916562848396	33.87574257194219
5	13.477364071275897	38.18204517219755	34.613969007570915	13.726621748955639
6	32.30276763432013	36.91796288178331	17.252599808384026	13.52666967551253
7	27.842803497174156	32.79221538677458	21.521691800281776	17.843289315769493
8	25.800924719697942	35.49122855000093	20.298381884476253	18.40946484582487
9	26.273810907680133	15.76902992178466	19.205962979344715	38.75119619119049
10-11	24.706910147057208	26.45802812978944	28.243256347229234	20.59180537592411
12-13	25.234876149016927	24.166915507905955	27.73330215322624	22.864906189850874
14-15	22.71996370248995	25.643535999228572	25.112206369047236	26.52429392923425
16-17	22.904647714840703	28.059414123717772	26.003951850250672	23.031986311190845
18-19	22.81445313589198	27.51976173484835	27.254311099294114	22.411474029965557
20-21	23.886420674309786	26.817990397019265	26.69789467302788	22.59769425564307
22-23	23.41831595910762	26.75215289318628	26.438517543291677	23.39101360441442
24-25	23.02584513998079	26.74618251782127	26.857708814552815	23.370263527645122
26-27	23.03896620242564	26.88165137037007	26.746273973268135	23.333108453936156
28-29	23.06135577194986	27.151272857577673	26.73105248653172	23.056318883940747
30-31	23.347580517938916	27.06065069837947	26.53009132384772	23.06167745983389
32-33	22.903429149570332	27.16295332574402	26.612590434878975	23.321027089806673
34-35	23.162144980574062	27.165140449953608	26.68365634346226	22.98905822601007
36-37	23.265539168412925	26.933371325552486	26.601389126490165	23.199700379544428
38-39	23.094590353091633	27.195780995232578	26.583597215989265	23.126031435686528
40-41	23.207640034193645	27.061710459282796	26.727509332734655	23.003140173788903
42-43	23.010359679361674	26.962812537379477	26.753172702393275	23.273655080865577
44-45	23.05485383850965	27.104442176167197	26.705109469358025	23.135594515965124
46-47	22.905201548681163	27.077731161956887	26.568536047410017	23.448531241951937
48-49	22.873228675993587	27.078868172721837	26.695031471120434	23.35287168016414
50-51	22.78161965586764	27.186096577920782	26.626463335893252	23.40582043031832
52-53	22.934282219024013	27.068730716091032	26.49911509645979	23.49787196842516
54-55	22.86356120382856	27.060184951999112	26.545242148678266	23.531011695494062
56-57	22.846189480312127	27.084809642672425	26.51202410668106	23.55697677033439
58-59	22.794352241086532	26.879010354488447	26.725906939453203	23.600730464971818
60-61	22.833697135184092	26.922038477554462	26.669742568593964	23.57452181866749
62-63	22.73945772241688	26.833900641806313	26.804524844225526	23.622116791551285
64-65	22.71174392202988	26.821781168432995	26.721681572482808	23.744793337054315
66-67	22.778104926305247	26.997553520559812	26.6497188940805	23.574622659054437
68-69	22.98069131374434	26.838759114043476	26.588912682359567	23.59163688985262
70-71	22.765913626580975	26.653625843493934	26.769626214033188	23.810834315891906
72-73	22.916710127360815	26.640800060425352	26.756057821306413	23.686431990907423
74-75	22.774827763244573	26.772008509569666	26.68783745325979	23.765326273925968
76-77	22.68352847291732	26.666901731250658	26.878118053751592	23.771451742080423
78-79	22.736427015728616	26.722279589023874	26.82883135341425	23.71246204183326
80-81	22.72594401064504	26.716214959432605	26.759172163587795	23.79866886633456
82-83	22.761574560872504	26.594051565380845	26.867761373978265	23.776612499768383
84-85	22.85128063051961	26.51041001405033	26.742483255645766	23.895826099784298
86-87	22.735157212781797	26.3950580375155	26.864527924545207	24.0052568251575
88-89	22.78221698361763	26.410570119679615	26.831976453615358	23.975236443087393
90-91	22.806009853563697	26.51554127322089	26.763967070360085	23.91448180285533
92-93	22.96441928570966	26.433954364664014	26.74371469246612	23.857911657160205
94-95	22.733312273692814	26.43685273986403	26.859928445194086	23.969906541249074
96-97	22.812930626822673	26.394972338255197	26.778659094739567	24.013437940182566
98-99	22.740195786597035	26.493045019909783	26.598875612901047	24.167883580592136
100-101	22.7953517539577	26.628204717684067	26.66535226125103	23.91109126710721
102-103	22.815519925042242	26.657392861927914	26.591319251177737	23.93576796185211
104-105	22.6274431585087	26.85124118108649	26.43473540876396	24.08658025164085
106-107	22.622142928839946	26.91116298999371	26.508939753219135	23.95775432794721
108-109	22.669331295464374	27.019195689389047	26.395314196650038	23.916158818496537
110-111	22.72479522860248	27.29083672505354	26.237361772336154	23.74700627400782
112-113	22.846485533098875	27.405749708032335	26.090856488247276	23.656908270621514
114-115	22.85550380529606	27.501280364701113	26.06630049525424	23.576915334748584
116-117	22.85550956268721	27.554055211108704	26.031457826826177	23.55897739937791
118-119	22.93564203016297	27.50800353470339	25.930535128408188	23.62581930672545
120-121	22.91960280631527	27.744272874630727	25.93043802750431	23.405686291549692
122-123	23.17708675305425	27.854806257538378	25.644232697823643	23.32387429158373
124-125	23.320501166985853	28.022054341309715	25.43095351704274	23.22649097466169
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1665.0
1	1267.0
2	777.0
3	640.5
4	509.5
5	399.0
6	385.0
7	412.0
8	478.0
9	516.0
10	508.5
11	516.5
12	545.0
13	620.0
14	679.0
15	774.5
16	972.5
17	1161.0
18	1357.5
19	1688.0
20	2134.0
21	2743.5
22	3517.5
23	4707.0
24	6262.5
25	7801.0
26	10036.0
27	13063.5
28	15779.0
29	18843.0
30	22636.5
31	26957.5
32	32449.0
33	38117.0
34	43918.5
35	49664.0
36	56567.5
37	63063.5
38	68202.5
39	73914.5
40	80136.0
41	85626.5
42	90426.0
43	96966.0
44	103339.5
45	108677.0
46	114364.0
47	118553.0
48	121000.0
49	122090.5
50	121546.0
51	118226.5
52	111831.5
53	106411.0
54	100557.5
55	93168.0
56	84225.5
57	74657.5
58	66990.5
59	59142.0
60	49694.0
61	40618.5
62	32930.0
63	24763.0
64	18403.0
65	14414.5
66	10716.0
67	8003.5
68	6165.5
69	4783.5
70	3856.5
71	3097.0
72	2494.0
73	2187.5
74	1830.0
75	1401.5
76	978.0
77	646.0
78	465.5
79	319.0
80	217.5
81	171.5
82	128.5
83	88.0
84	67.0
85	48.5
86	38.5
87	33.0
88	21.5
89	19.0
90	18.0
91	12.0
92	13.0
93	15.0
94	13.5
95	8.0
96	7.5
97	9.5
98	8.5
99	14.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2892413157514487
2	0.07217553896221064
3	0.08188041720912144
4	0.08658908035854852
5	0.09535941477427531
6	0.13054858441770373
7	0.11829168263179045
8	0.11926217045648153
9	0.12562425730723417
10-11	0.1035726172906424
12-13	0.09187284740408884
14-15	0.10251226948218364
16-17	0.10429149716078395
18-19	0.09638381858922701
20-21	0.09548521875155008
22-23	0.07641693019604573
24-25	0.08959040381638946
26-27	0.10202702556983811
28-29	0.09388571104048515
30-31	0.08959040381638946
32-33	0.0991335340925184
34-35	0.09255578328072331
36-37	0.09307697118657593
38-39	0.0827969890435519
40-41	0.09496403084569746
42-43	0.0840550288162996
44-45	0.07974174959545036
46-47	0.06951568344268695
48-49	0.07251700690052787
50-51	0.0744400105531565
52-53	0.08714621225790821
54-55	0.09251983928721623
56-57	0.10040954586201963
58-59	0.07041428328036387
60-61	0.0874697081994719
62-63	0.09370599107294977
64-65	0.08534901258255437
66-67	0.0788251777610199
68-69	0.08621166842672422
70-71	0.08531306858904729
72-73	0.06527429220885186
74-75	0.09514375081323286
76-77	0.09805521428730608
78-79	0.08038874147857775
80-81	0.09329263514761837
82-83	0.09713864245287564
84-85	0.10103856574839348
86-87	0.08919501988781162
88-89	0.06798806371863617
90-91	0.09359815909242854
92-93	0.06800603571538971
94-95	0.09697689448209379
96-97	0.0849895726474836
98-99	0.07877126177075927
100-101	0.09521563880024701
102-103	0.06734107183550878
104-105	0.0744939265434171
106-107	0.09183690341058177
108-109	0.0981989902613344
110-111	0.09140557548849684
112-113	0.08125139732274758
114-115	0.09433501095932362
116-117	0.08799089610532453
118-119	0.08053251745260605
120-121	0.11356504748560982
122-123	0.09268158725799808
124-125	0.11329546753430675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2782106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.94417150125021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06576899667152	35.18498380632164
2	14.881553151271	15.162567918866404
3	6.358403923136491	9.717708598034628
4	3.256394766104362	6.635773337607767
5	1.8802094447699444	4.789285620631524
6	1.1923858711570292	3.6447066189534807
7	0.7673136261442035	2.7363109875875544
8	0.5346201901249352	2.1788626123004557
9	0.3992299871803899	1.8304596841823733
>10	1.5794046471419632	13.389711797140505
>50	0.05978586353150217	2.07580269967705
>100	0.023656559491048345	2.067721288508612
>500	9.19369587983279E-4	0.306923331227158
>1k	3.5360368764275927E-4	0.2791816989607687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.5943993507077013E-5
5	0.0	0.0	0.0	0.0	3.5943993507077013E-5
6	0.0	0.0	0.0	0.0	3.5943993507077013E-5
7	0.0	0.0	0.0	0.0	3.5943993507077013E-5
8	0.0	0.0	0.0	0.0	7.188798701415403E-5
9	0.0	0.0	0.0	0.0	7.188798701415403E-5
10-11	0.0	0.0	0.0	0.0	7.188798701415403E-5
12-13	0.0	0.0	0.0	0.0	7.188798701415403E-5
14-15	0.0	0.0	0.0	1.7971996753538507E-5	1.0783198052123104E-4
16-17	0.0	0.0	0.0	5.391599026061552E-5	1.0783198052123104E-4
18-19	0.0	0.0	0.0	7.188798701415403E-5	1.0783198052123104E-4
20-21	0.0	0.0	0.0	7.188798701415403E-5	1.0783198052123104E-4
22-23	0.0	0.0	0.0	2.336359577960006E-4	1.7971996753538507E-4
24-25	0.0	0.0	0.0	5.751038961132322E-4	1.7971996753538507E-4
26-27	0.0	0.0	0.0	0.001240067775994157	1.7971996753538507E-4
28-29	0.0	0.0	0.0	0.00420544724032801	1.7971996753538507E-4
30-31	0.0	0.0	0.0	0.015869273133374502	1.9769196428892356E-4
32-33	0.0	0.0	0.0	0.047823483361165965	2.1566396104246208E-4
34-35	0.0	0.0	0.0	0.10139800568346426	2.1566396104246208E-4
36-37	0.0	0.0	0.0	0.193234909094046	2.1566396104246208E-4
38-39	0.0	0.0	0.0	0.34171954627178114	2.1566396104246208E-4
40-41	0.0	0.0	0.0	0.515023510966153	2.1566396104246208E-4
42-43	0.0	0.0	0.0	0.6845533563422816	2.3363595779600057E-4
44-45	0.0	0.0	0.0	0.8888050994462469	2.5160795454953907E-4
46-47	0.0	0.0	0.0	1.1370522906028744	2.5160795454953907E-4
48-49	0.0	0.0	0.0	1.4070815418247902	2.875519480566161E-4
50-51	0.0	0.0	0.0	1.7001868368782498	2.875519480566161E-4
52-53	0.0	0.0	0.0	1.9832817297399883	3.414679383172316E-4
54-55	0.0	1.7971996753538507E-5	0.0	2.300020200524351	4.1335592533138564E-4
56-57	0.0	3.5943993507077013E-5	0.0	2.6763358405466935	4.3132792208492416E-4
58-59	0.0	3.5943993507077013E-5	0.0	3.0963054606833817	4.3132792208492416E-4
60-61	0.0	3.5943993507077013E-5	0.0	3.5525785142622173	4.3132792208492416E-4
62-63	0.0	3.5943993507077013E-5	0.0	4.008096743977404	4.3132792208492416E-4
64-65	0.0	3.5943993507077013E-5	0.0	4.482629346257834	4.3132792208492416E-4
66-67	0.0	3.5943993507077013E-5	0.0	5.0350885264616085	4.492999188384627E-4
68-69	0.0	3.5943993507077013E-5	0.0	5.663766944897139	4.6727191559200115E-4
70-71	0.0	3.5943993507077013E-5	0.0	6.363866078431231	5.032159090990781E-4
72-73	0.0	3.5943993507077013E-5	0.0	7.054691661640498	5.032159090990781E-4
74-75	0.0	3.5943993507077013E-5	0.0	7.740556973745788	5.032159090990781E-4
76-77	0.0	3.5943993507077013E-5	0.0	8.509039554927096	5.032159090990781E-4
78-79	0.0	3.5943993507077013E-5	0.0	9.367795475801426	5.032159090990781E-4
80-81	0.0	3.5943993507077013E-5	0.0	10.286793529793616	5.032159090990781E-4
82-83	0.0	3.5943993507077013E-5	0.0	11.232390138981046	5.391599026061552E-4
84-85	0.0	3.5943993507077013E-5	0.0	12.154281684450556	5.391599026061552E-4
86-87	0.0	3.5943993507077013E-5	0.0	13.12651279282673	5.391599026061552E-4
88-89	0.0	3.5943993507077013E-5	0.0	14.175969571252857	5.391599026061552E-4
90-91	0.0	3.5943993507077013E-5	0.0	15.302903627683488	5.391599026061552E-4
92-93	0.0	3.5943993507077013E-5	0.0	16.43934487039674	5.391599026061552E-4
94-95	0.0	3.5943993507077013E-5	0.0	17.55276398526871	6.469918831273862E-4
96-97	0.0	3.5943993507077013E-5	0.0	18.674540078631082	6.469918831273862E-4
98-99	0.0	3.5943993507077013E-5	0.0	19.866604651296534	6.649638798809247E-4
100-101	0.0	3.5943993507077013E-5	0.0	21.14338921665817	6.829358766344632E-4
102-103	0.0	3.5943993507077013E-5	0.0	22.433113619682356	7.548238636486173E-4
104-105	0.0	3.5943993507077013E-5	0.0	23.676146775140847	7.548238636486173E-4
106-107	0.0	3.5943993507077013E-5	0.0	24.891413195615122	7.548238636486173E-4
108-109	0.0	3.5943993507077013E-5	0.0	26.135991942794416	7.907678571556942E-4
110-111	0.0	3.5943993507077013E-5	0.0	27.44650994606244	8.446838474163098E-4
112-113	0.0	5.391599026061552E-5	0.0	28.787202931879662	8.626558441698483E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6325	0.0	32.346645	1
CATGGGG	6325	0.0	30.961035	4
TACATGG	10460	0.0	30.557295	2
GTACATG	10880	0.0	29.769161	1
ACATGGG	10775	0.0	29.112095	3
AACGCAG	7205	0.0	27.589619	6
TCAACGC	7245	0.0	27.440258	4
CAACGCA	7400	0.0	26.865496	5
ATCAACG	7435	0.0	26.739027	3
GGTATCA	4730	0.0	26.229973	1
TATCAAC	7735	0.0	26.08625	2
ATGGGGA	3380	0.0	24.830332	5
ACGCAGA	8265	0.0	23.978762	7
CGCAGAG	8320	0.0	23.31952	8
ATGGGGC	2770	0.0	20.628687	5
GCAGAGT	9355	0.0	19.658033	9
GTATAGG	620	0.0	19.24128	1
TAGACCA	2830	0.0	18.719046	4
GTAGACC	2545	0.0	18.476486	3
TACGGTA	195	9.88818E-4	18.314571	5
>>END_MODULE