FastQCFastQC Report
Mon 15 Aug 2016
SRR2921097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.21293221753592423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47000.16893676948326197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.12425838555396523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85300.043.98931
GGTATCA54350.041.050411
ATCAACG108000.034.213593
TCAACGC108150.034.111034
CAACGCA109200.033.673895
AACGCAG111250.033.3327876
TATCAAC111800.033.2639272
TACATGG104750.029.8131582
GTACATG108200.029.3311981
ACGCAGA126950.029.052077
CGCAGAG128200.028.5367958
ACATGGG107700.027.834473
CATGGGG64750.027.1526974
GCAGAGT142850.024.6940619
TATAGCG1701.3819828E-524.5402975
AGTACTT87850.023.43040312-13
GAGTACT81450.022.67858512-13
AGAGTAC131550.022.63284510-11
GTACTTT90350.020.70910514-15
ATACCGT3451.2787496E-920.7035946
Produced by FastQC (version 0.11.5)