FastQCFastQC Report
Mon 15 Aug 2016
SRR2921096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768007
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70350.040.69851
GGTATCA46500.037.841671
TCAACGC81050.034.663874
GTACATG162000.034.5373541
CAACGCA83800.033.4553035
TACATGG169450.032.2459752
AACGCAG87850.031.773126
ATCAACG89200.031.6301753
ACATGGG167500.031.3075583
TATCAAC92100.030.8917642
CATGGGG130100.029.4643364
ACGCAGA103700.026.5119637
CGCAGAG114500.023.9587948
CCGCGTA1501.6864162E-423.802639
GCAGAGT128250.020.2763169
TACCGTA3902.746674E-1019.8361517
AGAGTAC105700.019.61683710-11
GAGTACA113450.019.2432231
ATGGGGG72400.018.9093845
GTACCGT8850.018.8297066
Produced by FastQC (version 0.11.5)