##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921096_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4768007
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.068812398975087	31.0	31.0	34.0	26.0	34.0
2	30.27955915333178	31.0	31.0	34.0	26.0	34.0
3	30.48924991091666	31.0	31.0	34.0	26.0	34.0
4	33.93653302102954	37.0	35.0	37.0	30.0	37.0
5	34.024376642064496	37.0	35.0	37.0	30.0	37.0
6	34.01847753159758	37.0	35.0	37.0	30.0	37.0
7	33.87121830987245	37.0	35.0	37.0	29.0	37.0
8	33.8878428660025	37.0	35.0	37.0	29.0	37.0
9	35.406891600620554	39.0	37.0	39.0	29.0	39.0
10-11	35.35694735766957	39.0	36.0	39.0	29.0	39.0
12-13	35.28463454437042	39.0	35.0	39.0	29.0	39.0
14-15	36.53719164002905	40.0	37.0	41.0	28.5	41.0
16-17	36.505944202682585	40.0	37.0	41.0	28.5	41.0
18-19	36.51091179186608	40.0	37.0	41.0	28.5	41.0
20-21	36.53306675095066	40.0	37.0	41.0	28.5	41.0
22-23	36.476234200159524	40.0	37.0	41.0	28.0	41.0
24-25	36.45117341480413	40.0	37.0	41.0	28.0	41.0
26-27	36.386700564827194	40.0	37.0	41.0	29.0	41.0
28-29	36.275124700949476	40.0	36.0	41.0	28.0	41.0
30-31	36.18885962206012	40.0	36.0	41.0	28.0	41.0
32-33	36.07741777644202	40.0	36.0	41.0	27.5	41.0
34-35	35.985962583528085	40.0	36.0	41.0	27.0	41.0
36-37	35.87966596945013	40.0	35.5	41.0	27.0	41.0
38-39	35.74627019213688	40.0	35.0	41.0	27.0	41.0
40-41	35.60612106903366	40.0	35.0	41.0	26.0	41.0
42-43	35.43138464352087	40.0	35.0	41.0	26.0	41.0
44-45	35.24137863052634	39.0	35.0	41.0	25.0	41.0
46-47	35.06182771963212	39.0	35.0	41.0	24.5	41.0
48-49	34.83167390484116	39.0	34.0	41.0	24.0	41.0
50-51	34.645576652886625	39.0	34.0	41.0	24.0	41.0
52-53	34.440369424793204	38.5	34.0	40.5	23.0	41.0
54-55	34.19604365094263	38.0	33.5	40.0	22.5	41.0
56-57	33.9326750149486	38.0	33.0	40.0	22.0	41.0
58-59	33.637604768617166	37.0	33.0	40.0	21.0	41.0
60-61	33.31742749119286	37.0	33.0	40.0	20.0	41.0
62-63	33.016096872341	36.0	32.5	40.0	19.5	41.0
64-65	32.67419951354937	36.0	32.0	39.0	18.0	41.0
66-67	32.31719364086504	35.0	32.0	39.0	17.5	40.5
68-69	31.947768113595473	35.0	31.5	38.5	16.0	40.0
70-71	31.583206568278946	35.0	31.0	37.5	13.5	40.0
72-73	31.23597123494156	35.0	31.0	37.0	11.5	39.0
74-75	31.18012421122704	35.0	31.0	36.5	12.0	39.0
76-77	31.243170993666745	35.0	32.0	36.0	13.0	39.0
78-79	31.082012882950885	35.0	32.0	36.0	11.5	37.5
80-81	30.88317456329238	35.0	32.0	35.5	9.0	37.0
82-83	30.660082504073507	35.0	32.0	35.0	7.0	37.0
84-85	30.432141039222465	35.0	31.5	35.0	7.0	36.0
86-87	30.297699961430425	35.0	31.5	35.0	3.5	36.0
88-89	30.144590601481916	35.0	31.0	35.0	2.0	36.0
90-91	30.021040761894852	35.0	31.0	35.0	2.0	35.5
92-93	29.923665485390437	35.0	31.0	35.0	2.0	35.0
94-95	29.821510853486583	35.0	31.0	35.0	2.0	35.0
96-97	29.734057647146912	35.0	31.0	35.0	2.0	35.0
98-99	29.643907716578436	35.0	31.0	35.0	2.0	35.0
100-101	29.527905160374136	35.0	31.0	35.0	2.0	35.0
102-103	28.229045385210213	33.0	28.0	34.5	2.0	35.0
104-105	29.203646093640383	34.0	30.0	35.0	2.0	35.0
106-107	29.345095340673787	35.0	31.0	35.0	2.0	35.0
108-109	29.294695876075686	35.0	31.0	35.0	2.0	35.0
110-111	29.204875957606603	35.0	31.0	35.0	2.0	35.0
112-113	29.105377886399914	35.0	30.0	35.0	2.0	35.0
114-115	28.977580989289656	35.0	30.0	35.0	2.0	35.0
116-117	28.850233231620678	35.0	30.0	35.0	2.0	35.0
118-119	28.664631155113657	35.0	30.0	35.0	2.0	35.0
120-121	28.481808751539166	34.5	30.0	35.0	2.0	35.0
122-123	28.202682588343514	34.0	29.5	35.0	2.0	35.0
124-125	26.904878285623322	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	143550.0
3	22612.0
4	14890.0
5	13694.0
6	16242.0
7	18004.0
8	19006.0
9	20396.0
10	22110.0
11	23637.0
12	24801.0
13	24069.0
14	23455.0
15	21447.0
16	18258.0
17	16480.0
18	15841.0
19	16179.0
20	16678.0
21	18016.0
22	21250.0
23	27535.0
24	37215.0
25	47611.0
26	54504.0
27	60665.0
28	71149.0
29	84265.0
30	100826.0
31	122160.0
32	154100.0
33	202268.0
34	281490.0
35	440052.0
36	851070.0
37	1312907.0
38	389155.0
39	420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.840941190327705	17.07212574444121	11.134726637718074	25.952206427513012
2	17.131876623624652	19.685330051490073	36.79088868750475	26.391904637380527
3	19.541168366299623	23.24506408203239	28.5005631394088	28.713204412259184
4	13.56135951749144	15.244992655488904	34.844069249566864	36.34957857745279
5	14.667682962669385	36.822984803421875	33.65901827653439	14.850313957374345
6	34.628874944063554	34.74325723036954	16.363281669162415	14.264586156404494
7	29.995126669839966	31.15781799949062	20.382278211510926	18.464777119158487
8	27.685738017870626	33.926284298994936	19.315674633336386	19.07230304979805
9	27.065655566656545	14.991838042574887	18.22308706353896	39.71941932722961
10-11	26.26626288085453	25.06380661863182	26.920152793693354	21.749777706820304
12-13	26.50329828409041	22.48360146530696	26.8063262653743	24.20677398522833
14-15	23.796258615472123	23.921346511315694	23.78509024078239	28.49730463242979
16-17	24.410165577814517	26.67008792867288	24.6414419097318	24.278304583780805
18-19	24.530796741318373	25.985401888883313	25.726079801971842	23.75772156782647
20-21	25.18757932899777	25.26559503268882	25.40787686764588	24.138948770667522
22-23	24.926966878149123	24.990025435402075	25.347000463205976	24.736007223242822
24-25	24.58901978194054	25.155541981720454	25.45307135131901	24.802366885019996
26-27	24.47693937884665	25.39739087672819	25.388227403194406	24.73744234123075
28-29	24.5532072151859	25.50677099344884	25.3891761295861	24.55084566177916
30-31	24.650115946698627	25.47211742968288	25.139238367415906	24.738528256202585
32-33	24.37869103269563	25.53951110637144	25.15531412141981	24.926483739513124
34-35	24.75281256556548	25.414484036999944	25.30880258581068	24.52390081162389
36-37	24.880524542730143	25.195364794127933	25.219358020607096	24.704752642534828
38-39	24.614775441213528	25.42354577971747	25.23384445435839	24.727834324710614
40-41	24.73629112703872	25.36410192250853	25.3589169599914	24.54068999046135
42-43	24.63713056478074	25.30376819594556	25.268757677572644	24.790343561701054
44-45	24.56326305131533	25.379993468255552	25.3512596695844	24.70548381084471
46-47	24.660574607058166	25.39514545033664	25.312530758835827	24.631749183769365
48-49	24.56187608936777	25.526794905562767	25.112181925598215	24.799147079471247
50-51	24.495528353587893	25.715450833296956	25.174848804370818	24.61417200874433
52-53	24.681065923834904	25.448932083138693	25.16593073021066	24.70407126281574
54-55	24.36914364498235	25.475435583296	25.334813791053524	24.820606980668124
56-57	24.35624067774194	25.571291066482065	25.26874869449025	24.803719561285746
58-59	24.415910774146848	25.313925988326712	25.35323428817034	24.916928949356095
60-61	24.29089847356501	25.420687690757145	25.481129650680316	24.807284184997528
62-63	24.27265905023799	25.464663374494233	25.470142165171893	24.79253541009588
64-65	24.23306760364717	25.367949557536985	25.425765704498517	24.97321713431733
66-67	24.16719111575376	25.427222680295316	25.41610929193654	24.989476912014393
68-69	24.362009710212263	25.22688419646739	25.491505899414157	24.919600193906184
70-71	24.113414440501487	25.154042433861534	25.615474444189417	25.117068681447563
72-73	24.174986692191354	25.262360312604702	25.530436713329603	25.03221628187434
74-75	24.051410948248456	25.389574287262604	25.59067874538948	24.968336019099464
76-77	24.113183135829065	25.155875946668022	25.650450977735094	25.08048993976782
78-79	23.99695785791555	25.158487752723	25.76030464238354	25.084249746977903
80-81	23.95891376998928	25.264584653392134	25.754736022000763	25.02176555461782
82-83	23.988144320374573	25.16699470823054	25.767787298073902	25.07707367332098
84-85	23.791208618186744	25.264185463580226	25.74814497582092	25.196460942412113
86-87	23.830433707967764	25.215841174732667	25.80913742770615	25.14458768959341
88-89	23.753117520992635	25.06253929692399	25.783734913968594	25.400608268114784
90-91	23.689136395057126	25.09752220239906	25.813570170118854	25.39977123242496
92-93	23.778051512063634	25.05995064178378	25.91412333551908	25.247874510633505
94-95	23.707160132344146	24.875226743577024	26.0108286917507	25.406784432328127
96-97	23.726410381489167	24.711786224342866	26.045081915598345	25.516721478569625
98-99	23.642726811073214	24.821898510196842	25.921460404406478	25.61391427432347
100-101	23.63305831792593	24.84947275447672	26.02706210387049	25.490406823726865
102-103	23.533530698553466	24.791385074249106	26.072251284617572	25.602832942579855
104-105	23.394489443791837	24.960369488798413	25.881331568386006	25.763809499023743
106-107	23.475598556180994	24.88868550124849	25.92220842591611	25.713507516654406
108-109	23.42069630058823	24.941292673812733	25.955586905316725	25.682424120282317
110-111	23.460236528778484	25.101329817074713	25.887733374505157	25.550700279641642
112-113	23.52428605116418	25.10438247396617	25.907905823894	25.46342565097565
114-115	23.479000341017546	25.2202890430716	25.840911588498354	25.4597990274125
116-117	23.385319578600644	25.179668405722143	25.873037580202034	25.561974435475175
118-119	23.486328939766736	25.202900079286746	25.751767947480797	25.55900303346572
120-121	23.450276423474335	25.332645642763573	25.809014485383706	25.408063448378382
122-123	23.61410008816491	25.350570972752845	25.623672278433183	25.41165666064906
124-125	23.670813333638478	25.49583951057166	25.479871948762707	25.353475207027152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1361.0
1	1134.0
2	826.5
3	659.5
4	526.0
5	440.5
6	413.5
7	441.0
8	496.0
9	545.0
10	516.0
11	515.0
12	583.0
13	650.5
14	744.0
15	867.5
16	943.0
17	1090.5
18	1338.5
19	1577.0
20	1880.0
21	2345.0
22	3076.0
23	4097.5
24	5294.0
25	6877.5
26	9181.0
27	12162.0
28	15398.0
29	19389.5
30	24032.0
31	29834.0
32	38409.0
33	43745.0
34	47009.5
35	53185.0
36	61400.0
37	71124.5
38	81071.0
39	90933.5
40	100260.5
41	110820.5
42	121182.5
43	133250.5
44	147209.5
45	161605.5
46	175728.0
47	186441.5
48	195871.5
49	204016.5
50	208537.0
51	212941.0
52	215460.0
53	216560.5
54	216157.5
55	207859.5
56	193983.5
57	177246.5
58	162641.0
59	147728.0
60	128080.0
61	108989.5
62	91061.5
63	70696.5
64	52917.0
65	40639.0
66	31504.5
67	25002.5
68	19714.5
69	15629.5
70	12359.0
71	9489.0
72	7158.0
73	5853.0
74	4912.5
75	3977.0
76	3007.5
77	2041.5
78	1323.5
79	802.5
80	510.5
81	335.0
82	234.0
83	167.5
84	131.5
85	102.0
86	82.0
87	60.0
88	46.5
89	37.0
90	35.0
91	34.0
92	24.5
93	18.5
94	15.5
95	11.5
96	8.0
97	13.0
98	14.5
99	13.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2852764268173264
2	0.0672188610461352
3	0.07644703541752351
4	0.08190004754607114
5	0.09024735072746327
6	0.12386726781231656
7	0.11220621110665314
8	0.11180772175879775
9	0.11889663752590966
10-11	0.09627712375422268
12-13	0.08579056196855415
14-15	0.09556403755279722
16-17	0.09768232303350227
18-19	0.08892604394246904
20-21	0.08855901427997065
22-23	0.07105694265968988
24-25	0.08262362030928226
26-27	0.09499776321637111
28-29	0.08795079369640187
30-31	0.0831479483985657
32-33	0.09301580303887977
34-35	0.08730062686569041
36-37	0.08730062686569041
38-39	0.07708671568644929
40-41	0.08873728583032701
42-43	0.07811439874144481
44-45	0.07514670175610061
46-47	0.06535225304828621
48-49	0.06715594167542119
50-51	0.06960979713326763
52-53	0.08170080287214343
54-55	0.08735305967461877
56-57	0.09525992726101283
58-59	0.06522641430685819
60-61	0.0824768084442829
62-63	0.0877096027753315
64-65	0.07900575649322662
66-67	0.07305987596075257
68-69	0.08069409294071926
70-71	0.08004392611000781
72-73	0.06181828172651592
74-75	0.09046756852496232
76-77	0.0948719244749431
78-79	0.07552421798038468
80-81	0.08751035810140378
82-83	0.09192520061317024
84-85	0.09630858343957968
86-87	0.0845112014307026
88-89	0.06298229008472513
90-91	0.0885065814710423
92-93	0.06387364783650695
94-95	0.0915686575124575
96-97	0.08043192889607755
98-99	0.07379393528574937
100-101	0.0909080041199604
102-103	0.06433505655507636
104-105	0.07067942643540583
106-107	0.08917772142532508
108-109	0.09536479287886951
110-111	0.08874777239211268
112-113	0.07712866193359195
114-115	0.09065632663710435
116-117	0.08436438956570325
118-119	0.0766462800914512
120-121	0.10918608131238061
122-123	0.08823393086461492
124-125	0.10960554378380737
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4768007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.49183827952868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81278377413575	34.10521113404016
2	13.671789779539239	12.985968584031813
3	5.429390997588138	7.735552776413542
4	2.664357637706908	5.061409681952141
5	1.5595588640910316	3.703315868040836
6	0.9945713330933829	2.8340412545235716
7	0.685093239122312	2.2775436123156725
8	0.48817337968002195	1.8547400960107645
9	0.3724736602123478	1.5920512950770043
>10	2.132041257407393	18.526619225933725
>50	0.13710143545144246	4.429435932505836
>100	0.050627541743062096	3.909787026523205
>500	0.0012399744422605997	0.42149554248273513
>1k	7.971257868471615E-4	0.5628279701488988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.291937071401112E-5
2	0.0	0.0	0.0	0.0	6.291937071401112E-5
3	0.0	0.0	0.0	0.0	6.291937071401112E-5
4	0.0	0.0	0.0	2.097312357133704E-5	6.291937071401112E-5
5	0.0	0.0	0.0	2.097312357133704E-5	1.2583874142802225E-4
6	0.0	0.0	0.0	2.097312357133704E-5	1.677849885706963E-4
7	0.0	0.0	0.0	2.097312357133704E-5	1.677849885706963E-4
8	0.0	0.0	0.0	2.097312357133704E-5	1.677849885706963E-4
9	0.0	0.0	0.0	8.389249428534815E-5	1.8875811214203334E-4
10-11	0.0	0.0	0.0	8.389249428534815E-5	1.8875811214203334E-4
12-13	0.0	0.0	0.0	8.389249428534815E-5	3.1459685357005556E-4
14-15	0.0	0.0	0.0	8.389249428534815E-5	4.5092215678374634E-4
16-17	0.0	0.0	0.0	1.048656178566852E-4	5.03354965712089E-4
18-19	0.0	0.0	0.0	1.4681186499935928E-4	5.557877746404315E-4
20-21	0.0	0.0	0.0	1.677849885706963E-4	6.606533924971167E-4
22-23	0.0	0.0	0.0	2.516774828560445E-4	7.235727632111278E-4
24-25	0.0	0.0	0.0	3.670296624983982E-4	7.86492133925139E-4
26-27	0.0	0.0	0.0	6.816265160684538E-4	8.598980664248186E-4
28-29	0.0	0.0	0.0	0.0023070435928470743	8.808711899961556E-4
30-31	0.0	0.0	0.0	0.01004612619067044	9.018443135674927E-4
32-33	0.0	0.0	0.0	0.030536867919866727	9.437905607101668E-4
34-35	0.0	0.0	0.0	0.062080445771157636	0.0010276830549955148
36-37	0.0	0.0	0.0	0.11623305083234986	0.0010276830549955148
38-39	0.0	0.0	0.0	0.20924885387122966	0.0010486561785668519
40-41	0.0	0.0	0.0	0.31474366543505494	0.0010801158639238573
42-43	0.0	0.0	0.0	0.422493087782799	0.001090602425709526
44-45	0.0	0.0	0.0	0.5571090814254258	0.0011220621110665315
46-47	0.0	0.0	0.0	0.7184133748125789	0.0011744949199948742
48-49	0.0	0.0	0.0	0.8949126962271657	0.0012583874142802222
50-51	0.0	0.0	0.0	1.0854849835581197	0.0013108202232085648
52-53	0.0	0.0	0.0	1.2813424980290506	0.0014471455264222556
54-55	0.0	0.0	0.0	1.5018224595727312	0.0015100648971362667
56-57	0.0	0.0	0.0	1.7643220741915857	0.0015415245824932722
58-59	0.0	0.0	0.0	2.0582918607292315	0.001572984267850278
60-61	0.0	0.0	0.0	2.3892792103702867	0.001593957391421615
62-63	0.0	0.0	0.0	2.726086601802388	0.0016568767621356261
64-65	0.0	0.0	0.0	3.0883553652500932	0.0016988230092783003
66-67	0.0	0.0	0.0	3.5165741157678667	0.0017722289417779796
68-69	0.0	0.0	0.0	3.999385906941831	0.0017827155035636483
70-71	0.0	0.0	0.0	4.527237480985242	0.001835148312491991
72-73	0.0	0.0	0.0	5.071280306425725	0.0019609870539200134
74-75	0.0	0.0	0.0	5.636086524201831	0.002044879548205361
76-77	0.0	0.0	0.0	6.26869885048407	0.0020553661099910296
78-79	0.0	0.0	0.0	6.9770870722295495	0.0020553661099910296
80-81	0.0	0.0	0.0	7.727725651409488	0.0020868257953480353
82-83	0.0	0.0	0.0	8.505000097525024	0.002223151098561726
84-85	0.0	0.0	0.0	9.281697782742349	0.002223151098561726
86-87	0.0	0.0	0.0	10.10876242421624	0.0022965570310614055
88-89	0.0	0.0	0.0	11.007376876753746	0.0023804495253467537
90-91	0.0	0.0	0.0	11.972685862248104	0.0024119092107037594
92-93	0.0	0.0	0.0	12.952162192714901	0.0025167748285604445
94-95	0.0	0.0	0.0	13.920501794565318	0.0025901807610601243
96-97	0.0	0.0	0.0	14.914271308745981	0.002726506064273815
98-99	0.0	0.0	0.0	15.966230754275319	0.002789425434987826
100-101	0.0	0.0	0.0	17.08940444089113	0.002789425434987826
102-103	0.0	0.0	0.0	18.22006553262191	0.0028418582439161686
104-105	0.0	0.0	0.0	19.33305676774384	0.0028733179292731743
106-107	0.0	0.0	0.0	20.430852974838334	0.0028733179292731743
108-109	0.0	1.048656178566852E-5	0.0	21.57367847824049	0.0029152641764158484
110-111	0.0	2.097312357133704E-5	0.0	22.770058852682055	0.002967696985344191
112-113	2.097312357133704E-5	2.097312357133704E-5	0.0	23.978970668457492	0.0029991566707011966
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7035	0.0	40.6985	1
GGTATCA	4650	0.0	37.84167	1
TCAACGC	8105	0.0	34.66387	4
GTACATG	16200	0.0	34.537354	1
CAACGCA	8380	0.0	33.455303	5
TACATGG	16945	0.0	32.245975	2
AACGCAG	8785	0.0	31.77312	6
ATCAACG	8920	0.0	31.630175	3
ACATGGG	16750	0.0	31.307558	3
TATCAAC	9210	0.0	30.891764	2
CATGGGG	13010	0.0	29.464336	4
ACGCAGA	10370	0.0	26.511963	7
CGCAGAG	11450	0.0	23.958794	8
CCGCGTA	150	1.6864162E-4	23.80263	9
GCAGAGT	12825	0.0	20.276316	9
TACCGTA	390	2.746674E-10	19.836151	7
AGAGTAC	10570	0.0	19.616837	10-11
GAGTACA	11345	0.0	19.243223	1
ATGGGGG	7240	0.0	18.909384	5
GTACCGT	885	0.0	18.829706	6
>>END_MODULE