FastQCFastQC Report
Mon 15 Aug 2016
SRR2921096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768007
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.12156022421946948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48460.1016357568266993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.052.0528261
GTATCAA99000.048.2087021
TCAACGC124650.037.5793154
CAACGCA129000.036.312495
ATCAACG133800.035.1875953
TATCAAC135750.034.945912
AACGCAG135100.034.6762246
GTACATG157600.032.0246771
ACGCAGA156050.029.8482427
TACATGG164250.029.6442092
ACATGGG163700.029.2338143
CGCAGAG168700.027.6106438
CATGGGG125700.026.6452434
TATACCG4150.025.8492285
GCAGAGT186150.024.4146829
AGAGTAC165100.022.9878810-11
AGTACTT110000.021.49738912-13
CAGAGTA175900.021.47500210-11
GAGTACT104650.020.9194312-13
GTACTTT113150.019.34904514-15
Produced by FastQC (version 0.11.5)