##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921096_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4768007 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44008848980297 33.0 31.0 34.0 28.0 34.0 2 31.54727939787001 33.0 31.0 34.0 28.0 34.0 3 31.639276536297032 34.0 31.0 34.0 28.0 34.0 4 35.11351556321121 37.0 35.0 37.0 32.0 37.0 5 34.87232925622802 37.0 35.0 37.0 32.0 37.0 6 34.66159424682053 37.0 35.0 37.0 32.0 37.0 7 34.60641165166075 37.0 35.0 37.0 31.0 37.0 8 34.604858591860285 37.0 35.0 37.0 31.0 37.0 9 36.19582899102287 39.0 37.0 39.0 32.0 39.0 10-11 36.15281426809986 39.0 37.0 39.0 31.0 39.0 12-13 36.103103351148604 39.0 37.0 39.0 31.0 39.0 14-15 37.39709736164397 40.0 38.0 41.0 31.0 41.0 16-17 37.37758868642601 40.0 38.0 41.0 31.0 41.0 18-19 37.375439046125564 40.0 38.0 41.0 31.0 41.0 20-21 37.36934866077168 40.0 38.0 41.0 31.0 41.0 22-23 37.33506410540085 40.0 38.0 41.0 31.0 41.0 24-25 37.2928683829533 40.0 38.0 41.0 31.0 41.0 26-27 37.187540727184334 40.0 37.0 41.0 31.0 41.0 28-29 37.07068487944754 40.0 37.0 41.0 30.0 41.0 30-31 36.96172237163242 40.0 37.0 41.0 30.0 41.0 32-33 36.86721884007301 40.0 37.0 41.0 30.0 41.0 34-35 36.741823785074146 40.0 37.0 41.0 30.0 41.0 36-37 36.58246799973238 40.0 36.0 41.0 30.0 41.0 38-39 36.45127398093166 40.0 36.0 41.0 30.0 41.0 40-41 36.32584400568204 40.0 36.0 41.0 29.0 41.0 42-43 36.178691432290265 40.0 35.5 41.0 28.5 41.0 44-45 36.023450468927585 40.0 35.0 41.0 28.0 41.0 46-47 35.792508693884045 40.0 35.0 41.0 27.0 41.0 48-49 35.571287332422116 39.5 35.0 41.0 26.5 41.0 50-51 35.37980302881267 39.0 35.0 41.0 26.0 41.0 52-53 35.16236112908391 39.0 35.0 41.0 26.0 41.0 54-55 34.93625449794851 39.0 35.0 41.0 26.0 41.0 56-57 34.67798621101018 38.0 34.0 41.0 25.0 41.0 58-59 34.40776995503572 38.0 34.0 40.0 24.0 41.0 60-61 34.10939109359529 37.0 34.0 40.0 23.5 41.0 62-63 33.807736544849874 37.0 34.0 40.0 23.0 41.0 64-65 33.479961543680616 36.0 33.5 39.5 22.5 41.0 66-67 33.14334332982313 36.0 33.0 39.0 22.0 41.0 68-69 32.791803158007106 35.0 33.0 39.0 22.0 40.5 70-71 32.417219500726404 35.0 33.0 37.5 20.5 40.0 72-73 32.05193511670599 35.0 32.0 37.0 20.0 39.5 74-75 31.73856519086486 35.0 32.0 36.5 20.0 39.0 76-77 31.45551726329261 35.0 32.0 36.0 19.0 39.0 78-79 31.175370233307124 35.0 32.0 36.0 18.0 37.0 80-81 31.15944439678885 35.0 32.0 35.0 19.0 37.0 82-83 30.986643266253594 35.0 32.0 35.0 18.0 37.0 84-85 30.813769988173256 35.0 32.0 35.0 18.0 36.0 86-87 30.664746192696448 35.0 32.0 35.0 17.0 36.0 88-89 30.509863555149984 35.0 32.0 35.0 15.5 36.0 90-91 30.377423313346647 35.0 32.0 35.0 13.0 35.0 92-93 30.288039950444706 35.0 32.0 35.0 10.5 35.0 94-95 30.191971509270015 35.0 31.5 35.0 8.5 35.0 96-97 30.127230203311363 35.0 31.0 35.0 7.0 35.0 98-99 30.039941006798017 35.0 31.0 35.0 7.0 35.0 100-101 29.95435765929035 35.0 31.0 35.0 3.5 35.0 102-103 28.601722166095815 33.0 28.0 34.5 2.0 35.0 104-105 29.641554846710587 34.0 31.0 35.0 2.0 35.0 106-107 29.815524075363143 35.0 31.0 35.0 2.0 35.0 108-109 29.814074413061896 35.0 31.0 35.0 2.0 35.0 110-111 29.72616179045039 35.0 31.0 35.0 2.0 35.0 112-113 29.621073542886997 35.0 31.0 35.0 2.0 35.0 114-115 29.536151373099912 35.0 31.0 35.0 2.0 35.0 116-117 29.43308325679891 35.0 31.0 35.0 2.0 35.0 118-119 29.287364196403235 35.0 31.0 35.0 2.0 35.0 120-121 29.44741744296936 35.0 31.0 35.0 2.0 35.0 122-123 29.376009724817937 35.0 31.5 35.0 2.0 35.0 124-125 28.67986980723812 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73926.0 3 28820.0 4 13616.0 5 11483.0 6 12597.0 7 14844.0 8 16591.0 9 18592.0 10 20723.0 11 22928.0 12 24253.0 13 26945.0 14 31718.0 15 29265.0 16 19975.0 17 17834.0 18 16838.0 19 16245.0 20 16625.0 21 17146.0 22 18292.0 23 21488.0 24 27794.0 25 36348.0 26 45201.0 27 54254.0 28 65334.0 29 78802.0 30 94148.0 31 116400.0 32 146009.0 33 189836.0 34 264318.0 35 419740.0 36 840574.0 37 1426920.0 38 470868.0 39 717.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.756525467902684 17.381253470546547 11.681646961980983 26.18057409956978 2 17.248532060577293 19.945181825637764 36.13175072266899 26.67453539111595 3 19.520587952156948 23.217184874099388 28.97929889784138 28.28292827590228 4 13.735305159789673 15.380204411650583 34.73582321517336 36.14866721338638 5 14.571244223061045 36.693297364598465 33.739927900105265 14.995530512235222 6 34.716139103079286 34.72438791408559 16.150584248800932 14.408888734034194 7 29.78498617871667 30.961520431357435 20.567839858949288 18.6856535309766 8 28.035286610897092 33.416626918154144 19.31337223863203 19.234714232316737 9 27.27119223564898 14.207717785054003 18.41598093731843 40.105109041978594 10-11 26.464709874875624 24.592636840630437 27.044030961445852 21.898622323048087 12-13 26.782756580660582 22.011263108646933 27.142545959249393 24.063434351443096 14-15 23.960662617729973 23.3669248458995 24.279671340364175 28.392741196006355 16-17 24.308901810152793 26.271364651671785 25.0821883771764 24.33754516099902 18-19 24.408157306017706 25.3264735901601 26.587056809577515 23.678312294244677 20-21 24.968223523466342 24.656615811018128 26.403582544681637 23.971578120833893 22-23 24.733797896396577 24.450430091804247 26.328535048763595 24.48723696303558 24-25 24.35661857258419 24.666461636060326 26.419190109524944 24.55772968183054 26-27 24.242355904997765 24.88488059898905 26.374129845642823 24.498633650370362 28-29 24.344554831799194 24.95266890723232 26.38722319570728 24.315553065261213 30-31 24.40108457935838 24.8731073618184 26.22791900269047 24.497889056132752 32-33 24.18846521127659 24.996307820372106 26.156260594038137 24.65896637431317 34-35 24.52583636170256 24.89862838631747 26.28395128288664 24.291583969093328 36-37 24.674771612445763 24.690389917434864 26.169377318424093 24.46546115169528 38-39 24.506070885895916 24.91029315685542 26.13229487258959 24.451341084659074 40-41 24.570439814581718 24.82981016779616 26.219377368497014 24.380372649125103 42-43 24.53896263682597 24.80046310893444 26.068159834257514 24.592414419982074 44-45 24.4530522506729 24.87035229077592 26.09032105385228 24.5862744046989 46-47 24.60791873303796 24.889035088041343 25.966602328083976 24.536443850836726 48-49 24.54416331226048 25.151270800587273 25.639018835928578 24.665547051223665 50-51 24.520305396928745 25.337874404485216 25.605719710763147 24.536100487822885 52-53 24.649125383084098 25.117930118748205 25.61796892535078 24.61497557281692 54-55 24.296512312096496 25.14996934221161 25.780284241645045 24.77323410404685 56-57 24.319045396064627 25.229026556246293 25.680907407374388 24.771020640314696 58-59 24.295452028312813 24.974776142195015 25.781394211245246 24.948377618246926 60-61 24.204199038736355 25.1250612917013 25.940105880594587 24.730633788967758 62-63 24.14167746769498 25.16771048055993 25.878176566869293 24.8124354848758 64-65 24.03381281463633 25.054736350317675 25.90583637971949 25.00561445532651 66-67 23.961190522865223 25.147788513669354 25.86587690893169 25.025144054533726 68-69 24.150940388204468 24.992044474586056 25.885171508961268 24.97184362824821 70-71 23.889435844548604 24.90689224968842 25.961060999343584 25.242610906419394 72-73 23.946162534941546 24.993706115893062 25.875521238502124 25.184610110663264 74-75 23.781948106895776 25.11322629547642 25.87442416685773 25.230401430770073 76-77 23.891166362197577 24.838238451158272 25.88238267627334 25.38821251037081 78-79 23.71300099080399 24.87899700052891 25.953338134752492 25.45466387391461 80-81 23.657708829167177 24.97623353810668 25.88975298402298 25.476304648703163 82-83 23.588567134996826 24.922772668095373 25.90605309363992 25.582607103267886 84-85 23.35997706033524 24.97746884838205 25.849096399347342 25.813457691935366 86-87 23.354456227240416 24.97661770946054 25.901946031638822 25.766980031660218 88-89 23.25172015942941 24.8064610866373 25.83716173694147 26.104657016991816 90-91 23.08843054945039 24.821189827478193 25.914173005115337 26.176206617956083 92-93 23.117873689425902 24.813651639162867 25.94213556952066 26.126339101890572 94-95 22.966984849811716 24.646704489261253 26.07296485057733 26.313345810349702 96-97 22.921231453548412 24.4456019351305 26.099917411664357 26.533249199656723 98-99 22.833827460551113 24.54665846862308 25.91944268407471 26.700071386751095 100-101 22.74860741414555 24.48312994894551 26.022484869947 26.745777766961936 102-103 22.55816317101498 24.38026813905438 26.08510960099676 26.97645908893388 104-105 22.39697625644346 24.563532396660566 25.88198376220847 27.157507584687508 106-107 22.40335046815723 24.448673668135417 25.904749118698074 27.243226745009274 108-109 22.309158081715584 24.473542157686644 25.936573249771712 27.280726510826064 110-111 22.26254545279093 24.570923660708807 25.879185755344174 27.287345131156087 112-113 22.130251814833255 24.64402648655435 25.860281779129707 27.365439919482686 114-115 22.002075980204246 24.76764785066199 25.773723191941748 27.45655297719201 116-117 21.888040219303267 24.68034354547556 25.801694906010788 27.629921329210383 118-119 21.876288633845718 24.676192401868764 25.69640237551194 27.751116588773577 120-121 21.72686756961904 24.780230716586395 25.83160805907747 27.661293654717095 122-123 21.660886024430027 24.794136391136295 25.652984653970538 27.89199293046314 124-125 21.54256568620789 24.881396115581218 25.693187795565915 27.882850402644983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 59.0 1 69.5 2 181.5 3 302.5 4 260.0 5 229.0 6 364.0 7 727.5 8 1162.0 9 1295.0 10 1102.0 11 977.5 12 1033.5 13 1170.5 14 1335.0 15 1577.0 16 1789.0 17 2008.0 18 2349.0 19 2604.0 20 2921.0 21 3371.0 22 4039.0 23 5002.5 24 6103.0 25 7668.0 26 9845.5 27 12573.0 28 15848.0 29 19918.0 30 24515.5 31 30300.5 32 38556.5 33 43657.5 34 46737.0 35 52461.5 36 60610.5 37 70159.5 38 79625.5 39 89440.0 40 98541.0 41 108494.5 42 119023.5 43 131627.0 44 145623.5 45 159724.5 46 173922.0 47 185370.5 48 195064.0 49 202874.5 50 208079.0 51 212914.5 52 215901.5 53 218065.5 54 218317.5 55 210068.5 56 195687.5 57 178757.5 58 164057.5 59 149260.0 60 129744.5 61 109979.0 62 91299.5 63 70584.5 64 52439.0 65 39967.5 66 30714.0 67 24226.5 68 19054.0 69 15096.0 70 11886.0 71 9064.5 72 6901.5 73 5815.0 74 4730.5 75 3658.0 76 2763.0 77 1848.5 78 1148.0 79 632.5 80 374.0 81 223.0 82 146.0 83 91.5 84 56.5 85 43.0 86 30.0 87 22.0 88 20.5 89 19.0 90 12.0 91 12.5 92 15.5 93 12.0 94 11.5 95 16.0 96 21.0 97 21.0 98 11.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1742866568778108 2 0.012793605378515592 3 0.0 4 0.0037122428721266558 5 0.14335129961008866 6 0.07726498723680565 7 0.007550324485681334 8 0.011178674863522642 9 0.012101492300661472 10-11 0.005683716487832337 12-13 0.010392182729597502 14-15 0.012216844480303825 16-17 0.015887141105287807 18-19 0.020291497055268587 20-21 0.023406005905612134 22-23 0.025880834487029904 24-25 0.025985700104886593 26-27 0.023248707478827107 28-29 0.022147618491331914 30-31 0.024790232061320378 32-33 0.02464342019632102 34-35 0.023909360871324222 36-37 0.02442320239882198 38-39 0.01995592707812719 40-41 0.02665684005916938 42-43 0.02294459718704272 44-45 0.032141311873074015 46-47 0.02721262783380981 48-49 0.018749972472775316 50-51 0.014744105870649938 52-53 0.01629611701492888 54-55 0.018162725012777874 56-57 0.014901404297434966 58-59 0.014576320882079243 60-61 0.01771180285599413 62-63 0.014974810229934646 64-65 0.027128735339524458 66-67 0.02045928204383928 68-69 0.018456348742776595 70-71 0.025545264509888515 72-73 0.03093535726772213 74-75 0.016442928879928237 76-77 0.00877725221460455 78-79 0.013978586860296136 80-81 0.016463902003499575 82-83 0.01574032924028845 84-85 0.01506918928600566 86-87 0.010696293021381888 88-89 0.021119935436336397 90-91 0.01825710406884889 92-93 0.01522648771279069 94-95 0.012049059491733129 96-97 0.02076339233562367 98-99 0.02130869354847843 100-101 0.027999119967734947 102-103 0.02024955080812591 104-105 0.014083452478152821 106-107 0.01912748869705938 108-109 0.010643860212453546 110-111 0.023290653725969782 112-113 0.017481098496709424 114-115 0.013013823176014632 116-117 0.011430352346378686 118-119 0.00757129760925267 120-121 0.0034815385128419483 122-123 0.004750412488907839 124-125 7.340593249967964E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4768007.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.97662504029268 #Duplication Level Percentage of deduplicated Percentage of total 1 70.71713772921895 32.51335325299035 2 14.238410097359758 13.092680844324537 3 5.412571781777356 7.465553499433393 4 2.8479799149908067 5.237620186952679 5 1.6146705897393032 3.71185521340161 6 1.0640063834013922 2.9351653518074223 7 0.7300582278722447 2.3495929380323926 8 0.5197764456100699 1.9118053395672212 9 0.388850013399389 1.6090210136678849 >10 2.261857847530479 19.05413362700778 >50 0.14650015094098076 4.588058028793185 >100 0.055667490412061954 4.191298191431906 >500 0.001507997227636606 0.46919267196988007 >1k 9.596336776805873E-4 0.7488966595616983 >5k 4.569684179419238E-5 0.12177318105800733 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5796 0.12156022421946948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4846 0.1016357568266993 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.291937071401112E-5 2 0.0 0.0 0.0 0.0 6.291937071401112E-5 3 0.0 0.0 0.0 0.0 6.291937071401112E-5 4 0.0 0.0 0.0 4.194624714267408E-5 6.291937071401112E-5 5 0.0 0.0 0.0 4.194624714267408E-5 6.291937071401112E-5 6 0.0 0.0 0.0 4.194624714267408E-5 1.048656178566852E-4 7 0.0 0.0 0.0 4.194624714267408E-5 1.048656178566852E-4 8 0.0 0.0 0.0 4.194624714267408E-5 1.048656178566852E-4 9 0.0 0.0 0.0 4.194624714267408E-5 1.048656178566852E-4 10-11 0.0 1.048656178566852E-5 0.0 1.048656178566852E-4 1.2583874142802225E-4 12-13 0.0 2.097312357133704E-5 0.0 1.048656178566852E-4 2.726506064273815E-4 14-15 0.0 2.097312357133704E-5 0.0 1.2583874142802225E-4 3.7751622428406674E-4 16-17 0.0 2.097312357133704E-5 0.0 1.2583874142802225E-4 4.404355949980778E-4 18-19 0.0 2.097312357133704E-5 0.0 1.2583874142802225E-4 5.243280892834259E-4 20-21 0.0 2.097312357133704E-5 0.0 1.4681186499935928E-4 5.872474599974371E-4 22-23 0.0 2.097312357133704E-5 0.0 2.3070435928470743E-4 6.711399542827852E-4 24-25 0.0 2.097312357133704E-5 0.0 3.880027860697352E-4 7.340593249967963E-4 26-27 0.0 2.097312357133704E-5 0.0 7.445458867824648E-4 7.86492133925139E-4 28-29 0.0 2.097312357133704E-5 0.0 0.002328016716418411 8.07465257496476E-4 30-31 0.0 2.097312357133704E-5 0.0 0.010109045561384453 8.703846282104872E-4 32-33 0.0 2.097312357133704E-5 0.0 0.030547354481652397 9.752502460671723E-4 34-35 0.0 2.097312357133704E-5 0.0 0.06194412046794394 9.85736807852841E-4 36-37 0.0 2.097312357133704E-5 0.0 0.1153941258894964 0.001006709931424178 38-39 0.0 2.097312357133704E-5 0.0 0.20760246367087967 0.001006709931424178 40-41 0.0 2.097312357133704E-5 0.0 0.3126882993250639 0.0010381696167811832 42-43 0.0 2.097312357133704E-5 0.0 0.41946247142674076 0.0010591427403525205 44-45 0.0 2.097312357133704E-5 0.0 0.5531766207558001 0.001090602425709526 46-47 0.0 2.097312357133704E-5 0.0 0.7135895563911714 0.0011325486728522 48-49 0.0 2.097312357133704E-5 0.0 0.8891346006832624 0.0011535217964235372 50-51 0.0 2.097312357133704E-5 0.0 1.078993801812791 0.0011744949199948742 52-53 0.0 2.097312357133704E-5 0.0 1.273865579475869 0.0013108202232085648 54-55 0.0 2.097312357133704E-5 0.0 1.4941043500984792 0.001394712717493913 56-57 0.0 2.097312357133704E-5 0.0 1.7554923891680527 0.0014366589646365871 58-59 0.0 2.097312357133704E-5 0.0 2.0493048772789133 0.0014890917735649297 60-61 0.0 2.097312357133704E-5 0.0 2.3795371944714008 0.0015310380207076038 62-63 0.0 2.097312357133704E-5 0.0 2.715484687837077 0.001593957391421615 64-65 0.0 2.097312357133704E-5 0.0 3.0768935532183574 0.0016254170767786204 66-67 0.0 2.097312357133704E-5 0.0 3.504514569714348 0.0016463902003499577 68-69 0.0 2.097312357133704E-5 0.0 3.985386346957964 0.0016568767621356261 70-71 0.0 2.097312357133704E-5 0.0 4.5113293667563825 0.0016988230092783003 72-73 0.0 2.097312357133704E-5 0.0 5.05356850356973 0.0018875811214203336 74-75 0.0 2.097312357133704E-5 0.0 5.614463233799782 0.0019505004921343445 76-77 0.0 2.097312357133704E-5 0.0 6.241905685121687 0.0019505004921343445 78-79 0.0 2.097312357133704E-5 0.0 6.946623610242183 0.002002933301062687 80-81 0.0 2.097312357133704E-5 0.0 7.6951229308178455 0.0020553661099910296 82-83 0.0 2.097312357133704E-5 0.0 8.47058320174446 0.002181204851419052 84-85 0.0 2.097312357133704E-5 0.0 9.244785085256797 0.002181204851419052 86-87 0.0 2.097312357133704E-5 0.0 10.069479763767125 0.002254610783918732 88-89 0.0 2.097312357133704E-5 0.0 10.966059823318211 0.002328016716418411 90-91 0.0 2.097312357133704E-5 0.0 11.928180894029728 0.00233850327820408 92-93 0.0 2.097312357133704E-5 0.0 12.904217632230825 0.0024958017049891076 94-95 0.0 2.097312357133704E-5 0.0 13.871214954172675 0.002569207637488787 96-97 0.0 2.097312357133704E-5 0.0 14.864030191230842 0.002768452311416489 98-99 0.0 2.097312357133704E-5 0.0 15.913598700673049 0.0029152641764158484 100-101 0.0 2.097312357133704E-5 0.0 17.033836149988872 0.0029152641764158484 102-103 0.0 2.097312357133704E-5 0.0 18.160564781050027 0.003030616356058202 104-105 0.0 2.097312357133704E-5 0.0 19.27132237851161 0.003083049164986545 106-107 0.0 2.097312357133704E-5 0.0 20.36818528160718 0.003083049164986545 108-109 0.0 2.097312357133704E-5 0.0 21.50959509916827 0.00311450885034355 110-111 0.0 2.097312357133704E-5 0.0 22.705335793340907 0.0031354819739148874 112-113 0.0 2.097312357133704E-5 0.0 23.913136033567064 0.003145968535700556 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6040 0.0 52.052826 1 GTATCAA 9900 0.0 48.208702 1 TCAACGC 12465 0.0 37.579315 4 CAACGCA 12900 0.0 36.31249 5 ATCAACG 13380 0.0 35.187595 3 TATCAAC 13575 0.0 34.94591 2 AACGCAG 13510 0.0 34.676224 6 GTACATG 15760 0.0 32.024677 1 ACGCAGA 15605 0.0 29.848242 7 TACATGG 16425 0.0 29.644209 2 ACATGGG 16370 0.0 29.233814 3 CGCAGAG 16870 0.0 27.610643 8 CATGGGG 12570 0.0 26.645243 4 TATACCG 415 0.0 25.849228 5 GCAGAGT 18615 0.0 24.414682 9 AGAGTAC 16510 0.0 22.98788 10-11 AGTACTT 11000 0.0 21.497389 12-13 CAGAGTA 17590 0.0 21.475002 10-11 GAGTACT 10465 0.0 20.91943 12-13 GTACTTT 11315 0.0 19.349045 14-15 >>END_MODULE