FastQCFastQC Report
Mon 15 Aug 2016
SRR2921095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616303
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32760.20268476888306214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG45500.031.786274
TACATGG75900.030.2675132
GTACATG79750.029.9916971
ACATGGG82500.027.7748343
GTATCGC1107.7448966E-427.0500586
GTATCAA61650.025.8324451
ATGGGGC24750.024.0467325
TATACCG2001.8224364E-623.8062635
AACGCAG63950.023.7296056
GTATAAT8150.022.6877521
TCAACGC68050.022.3901254
GGTATCA47850.022.188371
CAACGCA69750.021.6730845
TAGACCG3056.9849193E-921.4646635
GTATACC2505.266047E-721.42633
TATCAAC71850.021.3709662
ACTATAC3358.8584784E-1021.3197023
ATCAACG71400.021.2562483
ACGCAGA73300.020.6189637
GACCGTG5800.020.5181927
Produced by FastQC (version 0.11.5)