FastQCFastQC Report
Mon 15 Aug 2016
SRR2921095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616303
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56000.3464696903983968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.30068619559575155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.21140838073059323No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT26850.16611984262851706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.039.8876151
GTATCAA79000.037.991111
TCAACGC96850.030.4688454
TATCAAC97400.030.2967952
ATCAACG97750.030.1883133
CAACGCA98050.029.9743525
AACGCAG98800.029.7637796
TATAGCG1004.44429E-429.7444387
CATGGGG42150.029.7010754
TACATGG73800.027.2222482
GTACATG76650.026.4671941
ACGCAGA111200.026.4276127
CGCAGAG115650.025.3592878
ACATGGG77600.025.274623
GAGTACT67650.024.79840712-13
GCAGAGT121550.023.981539
ATGGGGC25300.023.7985925
AGTACTT70500.023.03647612-13
GTACTTT76800.022.19351214-15
AGAGTAC107000.021.84931610-11
Produced by FastQC (version 0.11.5)