##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616303
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26151655970446	33.0	31.0	34.0	28.0	34.0
2	31.308986619464296	33.0	31.0	34.0	28.0	34.0
3	31.411808305744653	33.0	31.0	34.0	28.0	34.0
4	34.903170383275906	37.0	35.0	37.0	32.0	37.0
5	34.66161852078478	37.0	35.0	37.0	32.0	37.0
6	34.399566789147826	37.0	35.0	37.0	30.0	37.0
7	34.33976612058506	37.0	35.0	37.0	30.0	37.0
8	34.32584051381455	37.0	35.0	37.0	30.0	37.0
9	35.86864653471533	39.0	37.0	39.0	30.0	39.0
10-11	35.842938483687774	39.0	37.0	39.0	30.0	39.0
12-13	35.78095165324818	39.0	37.0	39.0	30.0	39.0
14-15	37.00483479892075	40.0	37.0	41.0	30.0	41.0
16-17	36.994975261445404	40.0	37.0	41.0	30.0	41.0
18-19	37.034334527622605	40.0	37.0	41.0	30.0	41.0
20-21	37.049527223546576	40.0	37.0	41.0	30.0	41.0
22-23	37.011813688398775	40.0	37.0	41.0	30.0	41.0
24-25	36.965437482947195	40.0	37.0	41.0	30.0	41.0
26-27	36.83903482205997	40.0	37.0	41.0	30.0	41.0
28-29	36.69916377065439	40.0	37.0	41.0	30.0	41.0
30-31	36.53980472720771	40.0	36.5	41.0	30.0	41.0
32-33	36.381575731778014	40.0	36.0	41.0	30.0	41.0
34-35	36.20792326686271	40.0	35.5	41.0	29.0	41.0
36-37	36.0077386480134	40.0	35.0	41.0	27.5	41.0
38-39	35.85297156535624	40.0	35.0	41.0	27.0	41.0
40-41	35.706353944773966	40.0	35.0	41.0	27.0	41.0
42-43	35.52538632917219	40.0	35.0	41.0	26.0	41.0
44-45	35.31924923730266	40.0	35.0	41.0	24.5	41.0
46-47	34.992493672287935	39.5	35.0	41.0	23.5	41.0
48-49	34.672931993568035	39.0	35.0	41.0	22.0	41.0
50-51	34.449571336562514	39.0	34.5	41.0	20.5	41.0
52-53	34.233871990585925	39.0	34.0	41.0	18.5	41.0
54-55	34.01586800247231	38.5	34.0	41.0	18.0	41.0
56-57	33.76261907575498	38.0	34.0	41.0	15.0	41.0
58-59	33.505818525363125	38.0	33.0	40.0	13.0	41.0
60-61	33.208958035714836	37.0	33.0	40.0	10.5	41.0
62-63	32.92054893172877	37.0	33.0	40.0	8.0	41.0
64-65	32.60635938929767	36.0	33.0	39.5	7.0	41.0
66-67	32.25888648353681	36.0	32.0	39.0	3.5	41.0
68-69	31.891563648647562	35.0	32.0	39.0	2.0	41.0
70-71	31.4992702482146	35.0	32.0	38.0	2.0	40.0
72-73	31.113209590033552	35.0	31.0	37.0	2.0	39.5
74-75	30.80133891974463	35.0	31.0	37.0	2.0	39.0
76-77	30.510474830523734	35.0	31.0	36.0	2.0	39.0
78-79	30.216428479066117	35.0	31.0	36.0	2.0	37.5
80-81	30.18295826958188	35.0	31.0	35.5	2.0	37.0
82-83	29.9932166184187	35.0	31.0	35.0	2.0	37.0
84-85	29.803384637657665	35.0	31.0	35.0	2.0	36.5
86-87	29.636037921107615	35.0	31.0	35.0	2.0	36.0
88-89	29.465922540513752	35.0	31.0	35.0	2.0	36.0
90-91	29.31870787841141	35.0	31.0	35.0	2.0	35.5
92-93	29.209779045141907	35.0	30.0	35.0	2.0	35.0
94-95	29.105006610765432	35.0	30.0	35.0	2.0	35.0
96-97	29.031833140197104	35.0	30.0	35.0	2.0	35.0
98-99	28.940988168678768	35.0	30.0	35.0	2.0	35.0
100-101	28.8425824242113	34.0	30.0	35.0	2.0	35.0
102-103	27.502765261216492	32.5	27.5	34.5	2.0	35.0
104-105	28.524831049623742	34.0	29.0	35.0	2.0	35.0
106-107	28.679931918705837	34.0	30.0	35.0	2.0	35.0
108-109	28.66397142120011	34.0	30.0	35.0	2.0	35.0
110-111	28.561437428501957	34.0	29.5	35.0	2.0	35.0
112-113	28.43643240159797	34.0	29.0	35.0	2.0	35.0
114-115	28.326420231850094	34.0	29.0	35.0	2.0	35.0
116-117	28.18817604124969	34.0	29.0	35.0	2.0	35.0
118-119	28.022364618515216	34.0	29.0	35.0	2.0	35.0
120-121	28.137451331835678	34.5	29.5	35.0	2.0	35.0
122-123	28.01623581717042	35.0	30.0	35.0	2.0	35.0
124-125	27.287271940966512	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29489.0
3	11860.0
4	5001.0
5	4773.0
6	5882.0
7	7091.0
8	7462.0
9	8037.0
10	9186.0
11	10722.0
12	11536.0
13	13541.0
14	17637.0
15	16410.0
16	9297.0
17	7676.0
18	7090.0
19	7039.0
20	7260.0
21	7515.0
22	7699.0
23	8082.0
24	9969.0
25	12808.0
26	15477.0
27	18448.0
28	22051.0
29	26779.0
30	32311.0
31	39933.0
32	49979.0
33	64466.0
34	88333.0
35	135687.0
36	262126.0
37	452546.0
38	164725.0
39	380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.345904325601346	18.317120694912354	12.251015768898013	25.085959210588292
2	16.804693233274385	20.962320727374433	36.99132610694543	25.241659932405756
3	19.197761805800027	24.49027193539825	30.14323428218595	26.16873197661577
4	13.308464945224788	16.658643940696997	35.71866094310134	34.31423017097687
5	13.619886037308934	37.565495710445305	34.16625517258898	14.648363079656788
6	32.36057321705535	36.21786953708606	16.59617545717125	14.825381788687352
7	27.641936366077584	32.69631761769405	21.070275501150242	18.59147051507813
8	26.86903512593062	33.72876405829241	20.084176500871216	19.31802431490575
9	26.6640884832547	14.611431665248666	19.452270090494235	39.272209761002394
10-11	25.38926864641055	25.36269455698302	27.599960649311118	21.64807614729532
12-13	26.200287037435217	23.256065050268678	27.577219040995494	22.966428871300618
14-15	23.681939036335276	24.452013219127267	25.59832267317929	26.267725071358168
16-17	22.942699019490153	26.9036593077895	26.49294782865891	23.660693844061438
18-19	22.727163030907207	25.763150583126187	28.84950845621155	22.66017792975506
20-21	23.827802656173084	24.882338580847495	28.61736732361926	22.67249143936016
22-23	23.509905804431025	24.830060896199225	28.319161556400534	23.340871742969213
24-25	23.04397723056911	25.064227382415854	28.706940270313503	23.184855116701534
26-27	22.937884362859577	25.10888089377012	28.81330655092309	23.13992819244721
28-29	22.89139739766623	25.15824459115687	28.882971712445027	23.067386298731876
30-31	23.210623031936734	25.02972690235931	28.62798935988844	23.13166070581552
32-33	22.7664035936433	25.326298937878892	28.57111031832692	23.336187150150884
34-35	23.214730494270462	25.18039502650889	28.604226260738535	23.000648218482116
36-37	23.32139000535284	24.953727339391634	28.56178815754054	23.163094497714987
38-39	23.077027796341675	25.236875324868198	28.468230984381577	23.217865894408554
40-41	23.21437025251536	25.14234138230106	28.49813558393771	23.14515278124587
42-43	23.349701110162254	24.993347689946656	28.249607049592196	23.40734415029889
44-45	23.129670177332134	25.280226111776056	28.05937228855863	23.53073142233318
46-47	23.432691870336043	25.21728394832527	27.68353295701415	23.66649122432454
48-49	23.370722760501813	25.318460893014716	27.489242314922645	23.82157403156083
50-51	23.08388658862155	25.7132143629497	27.320833615645903	23.88206543278285
52-53	23.29672022810639	25.587870214988296	27.17970803570103	23.93570152120429
54-55	23.026615616660866	25.683350736744405	27.081780562323548	24.208253084271185
56-57	23.14942636842734	25.680446901102467	26.818057220221185	24.35206951024901
58-59	22.8099219577845	25.508558346030984	27.287982736350347	24.39353695983417
60-61	23.03996990245214	25.708311817773705	27.088411856230994	24.163306423543162
62-63	22.6613427683405	25.684870265557585	27.190430843464146	24.463356122637766
64-65	22.494056010267848	25.621782788483078	27.2274335702678	24.656727630981276
66-67	22.576353455912223	25.76669045968167	27.00606211369225	24.65089397071386
68-69	22.75108652840908	25.52134335659078	26.945533267555323	24.78203684744482
70-71	22.520892644805084	25.244076278845835	27.240933215630907	24.994097860718174
72-73	22.5698727156491	25.135301897290002	27.233169805505653	25.061655581555247
74-75	22.367721262272326	25.33937715547847	27.12712291521705	25.165778667032157
76-77	22.243008348928083	25.00334891416292	27.25146803094056	25.50217470596843
78-79	22.31518971794073	25.11158832408832	27.053149363296065	25.520072594674886
80-81	22.10490393665122	25.18964227393756	27.065490396759053	25.63996339265217
82-83	22.03451236396544	25.021881550349224	27.135257218436504	25.80834886724883
84-85	21.954545777993385	24.946298502669528	26.95689865074118	26.14225706859591
86-87	21.808685730691362	24.93840368080546	27.078558664693276	26.1743519238099
88-89	21.827482471548077	24.86297315836389	26.984301821362482	26.32524254872555
90-91	21.754814348880604	24.801511854524843	26.904909164658914	26.538764631935635
92-93	21.71812536527668	24.77956548279638	26.920902844834334	26.58140630709261
94-95	21.489184699901216	24.734532699278752	26.965964683560006	26.810317917260022
96-97	21.41333314356383	24.594189773424493	26.973125853382633	27.01935122962904
98-99	21.43186003683241	24.54822744014733	26.775406443691956	27.2445060793283
100-101	21.28437431635062	24.66227913967009	26.858497458178064	27.194849085801227
102-103	21.11361386138614	24.562097772277227	26.812716584158412	27.51157178217822
104-105	21.00173966203592	24.67572767714744	26.64578742214757	27.67674523866907
106-107	20.784810103083405	24.730394985462755	26.792001220280792	27.692793691173044
108-109	20.734061948252396	24.7629041843641	26.712264137809633	27.79076972957387
110-111	20.702840720626167	24.960177681183815	26.53206083439361	27.80492076379641
112-113	20.555015141895712	24.98917110032759	26.52319302792123	27.93262072985546
114-115	20.423739196293326	25.092846287881038	26.491790172958307	27.991624342867325
116-117	20.273502433102443	25.111522996213527	26.495617816825394	28.11935675385864
118-119	20.26591609322315	25.035353283794908	26.514761755527594	28.183968867454347
120-121	20.256629740849004	25.159620196893005	26.500742300813883	28.0830077614441
122-123	20.128224681518763	25.251550603551298	26.33357783304372	28.286646881886224
124-125	19.978661100032976	25.43669263755362	26.283226411977502	28.301419850435906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	139.5
2	213.5
3	457.5
4	377.0
5	212.5
6	378.5
7	830.5
8	1298.5
9	1404.5
10	1065.5
11	728.0
12	752.0
13	809.5
14	869.0
15	974.5
16	1104.5
17	1273.0
18	1461.0
19	1649.0
20	1894.5
21	2156.0
22	2503.5
23	2965.0
24	3515.5
25	4178.0
26	5397.0
27	7105.0
28	8314.0
29	9763.0
30	11711.0
31	13460.5
32	15694.0
33	18448.5
34	21229.0
35	24208.5
36	28067.0
37	31625.0
38	33963.0
39	36338.0
40	39213.0
41	42096.0
42	44471.0
43	48485.5
44	53656.0
45	57267.0
46	60615.0
47	64367.5
48	68448.0
49	71508.0
50	72871.5
51	72563.5
52	70059.5
53	68138.0
54	66376.0
55	62456.0
56	57104.5
57	51402.5
58	46803.5
59	42407.5
60	36170.5
61	29760.0
62	24174.5
63	18060.5
64	12790.0
65	9369.5
66	6816.0
67	4989.0
68	3657.5
69	2695.0
70	2159.0
71	1635.0
72	1221.5
73	1104.0
74	879.5
75	628.5
76	469.0
77	303.0
78	169.5
79	95.5
80	48.5
81	35.5
82	28.0
83	17.0
84	13.5
85	8.5
86	5.5
87	6.0
88	3.0
89	4.5
90	5.5
91	4.0
92	2.0
93	3.5
94	5.5
95	11.0
96	10.5
97	3.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16995575705792787
2	0.011693352050945892
3	0.0
4	0.00377404484183968
5	0.1401964854362084
6	0.07473846178593989
7	0.007486220096108217
8	0.0108271778249499
9	0.011012786587663328
10-11	0.004145262367266533
12-13	0.008507068291032066
14-15	0.009899134011382767
16-17	0.01268326545208417
18-19	0.0168285278193507
20-21	0.02050976827983367
22-23	0.022056507969112227
24-25	0.022551464669681366
26-27	0.019612659260052106
28-29	0.01806591957077355
30-31	0.021190333743116234
32-33	0.020942855392831666
34-35	0.02075724663011824
36-37	0.020911920599046094
38-39	0.016271701531210422
40-41	0.023603247658390784
42-43	0.01998387678547896
44-45	0.028769358220581166
46-47	0.02397446518381764
48-49	0.015405527305214432
50-51	0.011538678082018037
52-53	0.013301961327795594
54-55	0.014879635810859722
56-57	0.012126439163943889
58-59	0.011786156432302608
60-61	0.013889722409721444
62-63	0.012002699988801605
64-65	0.023819791214889783
66-67	0.017478158488847696
68-69	0.016178897149853707
70-71	0.022489595082110223
72-73	0.029202445333579165
74-75	0.01345663529672345
76-77	0.006650980663897797
78-79	0.012776069833440883
80-81	0.014477483491647297
82-83	0.013332896121581164
84-85	0.011971765195016033
86-87	0.008352394322104209
88-89	0.017787506426703407
90-91	0.016983201788278557
92-93	0.014446548697861723
94-95	0.00887828581645892
96-97	0.019024898178126255
98-99	0.020726311836332667
100-101	0.026882335799661327
102-103	0.018746485034056116
104-105	0.011414938906875753
106-107	0.017416288901276555
108-109	0.008135850765605211
110-111	0.02320109533917836
112-113	0.015900484005783568
114-115	0.011817091226088178
116-117	0.00968259045488377
118-119	0.007207806952038077
120-121	0.002753196646915832
122-123	0.004794893036763528
124-125	3.7121752542685374E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1616303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.0407676809691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4253265714864	33.35245984996188
2	14.537379482533364	13.967737408209441
3	6.143799569817879	8.854585434361757
4	3.099354379006182	5.95581454731321
5	1.7917307775456564	4.303806101545651
6	1.169208063471215	3.3701791768761846
7	0.7428386740177255	2.498057811404728
8	0.5539839306798617	2.1291050650225065
9	0.4009284951362122	1.7334821422367388
>10	2.018524079088231	17.002750682234304
>50	0.08733297688363006	2.841311403004749
>100	0.02726705555964673	2.2126138435514413
>500	0.0012921915411203604	0.4235573317520857
>1k	9.045340787842523E-4	1.0069031102182073
>5k	1.2921915411203604E-4	0.3476360923071493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5600	0.3464696903983968	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4860	0.30068619559575155	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3417	0.21140838073059323	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	2685	0.16611984262851706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.18695875711423E-5	0.0
6	0.0	0.0	0.0	6.18695875711423E-5	0.0
7	0.0	0.0	0.0	6.18695875711423E-5	0.0
8	0.0	0.0	0.0	6.18695875711423E-5	0.0
9	0.0	0.0	0.0	1.237391751422846E-4	0.0
10-11	0.0	0.0	0.0	1.237391751422846E-4	0.0
12-13	0.0	0.0	0.0	1.237391751422846E-4	0.0
14-15	0.0	0.0	0.0	1.237391751422846E-4	0.0
16-17	0.0	0.0	0.0	2.474783502845692E-4	0.0
18-19	0.0	0.0	0.0	3.402827316412826E-4	0.0
20-21	0.0	0.0	0.0	5.258914943547095E-4	0.0
22-23	0.0	0.0	0.0	0.0010517829887094188	0.0
24-25	0.0	0.0	0.0	0.0021035659774188377	0.0
26-27	0.0	0.0	0.0	0.0040833927796953915	0.0
28-29	0.0	0.0	0.0	0.009651655661098197	0.0
30-31	0.0	0.0	0.0	0.030377967497430866	0.0
32-33	0.0	0.0	0.0	0.07319172209666133	0.0
34-35	0.0	0.0	0.0	0.1403202246113507	0.0
36-37	0.0	0.0	0.0	0.2531394175473287	0.0
38-39	0.0	0.0	0.0	0.42977709006293996	0.0
40-41	0.0	0.0	0.0	0.628502205341449	0.0
42-43	0.0	0.0	0.0	0.8113887062017455	0.0
44-45	0.0	0.0	0.0	1.0414198327912527	0.0
46-47	0.0	0.0	0.0	1.3269479794320742	0.0
48-49	0.0	0.0	0.0	1.6257780874006915	0.0
50-51	0.0	0.0	0.0	1.939240352829884	0.0
52-53	0.0	0.0	0.0	2.255146466968137	0.0
54-55	0.0	0.0	0.0	2.6099685516886377	0.0
56-57	0.0	0.0	0.0	3.0382298368560843	0.0
58-59	0.0	0.0	0.0	3.5065826147696315	0.0
60-61	0.0	0.0	0.0	4.004075968429187	0.0
62-63	0.0	0.0	0.0	4.5111591081622695	0.0
64-65	0.0	0.0	0.0	5.059230849661233	0.0
66-67	0.0	0.0	0.0	5.694353100872794	0.0
68-69	0.0	0.0	0.0	6.402914552531302	0.0
70-71	3.093479378557115E-5	0.0	0.0	7.179037593817496	0.0
72-73	6.18695875711423E-5	0.0	0.0	7.9534282866516985	0.0
74-75	6.18695875711423E-5	0.0	0.0	8.737285026384285	0.0
76-77	6.18695875711423E-5	0.0	0.0	9.605872166295551	0.0
78-79	6.18695875711423E-5	0.0	0.0	10.593620131868839	0.0
80-81	6.18695875711423E-5	0.0	0.0	11.626161678843633	0.0
82-83	6.18695875711423E-5	0.0	0.0	12.682151799507889	0.0
84-85	6.18695875711423E-5	0.0	0.0	13.731429069920678	0.0
86-87	6.18695875711423E-5	0.0	0.0	14.831872489254799	0.0
88-89	6.18695875711423E-5	0.0	0.0	16.01348880748226	0.0
90-91	6.18695875711423E-5	0.0	0.0	17.2708335008968	0.0
92-93	6.18695875711423E-5	0.0	0.0	18.560876271342686	0.0
94-95	6.18695875711423E-5	0.0	0.0	19.825397836915478	0.0
96-97	6.18695875711423E-5	0.0	0.0	21.095549534957243	0.0
98-99	6.18695875711423E-5	0.0	0.0	22.438954824683243	0.0
100-101	6.18695875711423E-5	0.0	0.0	23.84187865765268	0.0
102-103	6.18695875711423E-5	0.0	0.0	25.268715086218364	0.0
104-105	6.18695875711423E-5	0.0	0.0	26.671051158105875	0.0
106-107	6.18695875711423E-5	0.0	0.0	28.03549210760606	0.0
108-109	6.18695875711423E-5	0.0	0.0	29.401417927207955	0.0
110-111	6.18695875711423E-5	0.0	0.0	30.81943793954475	0.0
112-113	6.18695875711423E-5	0.0	0.0	32.26430935288742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5475	0.0	39.887615	1
GTATCAA	7900	0.0	37.99111	1
TCAACGC	9685	0.0	30.468845	4
TATCAAC	9740	0.0	30.296795	2
ATCAACG	9775	0.0	30.188313	3
CAACGCA	9805	0.0	29.974352	5
AACGCAG	9880	0.0	29.763779	6
TATAGCG	100	4.44429E-4	29.744438	7
CATGGGG	4215	0.0	29.701075	4
TACATGG	7380	0.0	27.222248	2
GTACATG	7665	0.0	26.467194	1
ACGCAGA	11120	0.0	26.427612	7
CGCAGAG	11565	0.0	25.359287	8
ACATGGG	7760	0.0	25.27462	3
GAGTACT	6765	0.0	24.798407	12-13
GCAGAGT	12155	0.0	23.98153	9
ATGGGGC	2530	0.0	23.798592	5
AGTACTT	7050	0.0	23.036476	12-13
GTACTTT	7680	0.0	22.193512	14-15
AGAGTAC	10700	0.0	21.849316	10-11
>>END_MODULE