FastQCFastQC Report
Mon 15 Aug 2016
SRR2921094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719677
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69600.032.7451861
GTACATG122700.029.4173931
TACATGG123400.029.088332
TCAACGC80800.027.7745254
CATGGGG79100.027.5436334
AACGCAG81900.027.3252776
GTATAGG5500.027.1188141
CAACGCA83650.027.041725
ACATGGG130800.026.896543
GGTATCA53050.026.878551
TATCAAC84700.026.8470522
ATCAACG85850.026.140733
ACGCAGA94550.023.415867
GTATACG1552.068981E-423.0947321
CGCAGAG97750.022.5884198
GCAGAGT106250.019.6610589
GTATAGA6800.019.3022141
ATGGGGA42350.019.2567865
TAATGCG1550.00567502719.2023754
ATGGGGC35100.019.1640825
Produced by FastQC (version 0.11.5)