##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921094_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2719677
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.448443326174395	31.0	30.0	34.0	24.0	34.0
2	29.635671074175352	31.0	30.0	34.0	25.0	34.0
3	29.841789668405475	31.0	30.0	34.0	25.0	34.0
4	33.211958993659906	37.0	35.0	37.0	26.0	37.0
5	33.34379670821204	37.0	35.0	37.0	27.0	37.0
6	33.321876090432795	37.0	35.0	37.0	27.0	37.0
7	33.16897116826741	37.0	35.0	37.0	26.0	37.0
8	33.18968759893178	37.0	35.0	37.0	26.0	37.0
9	34.620627743662205	39.0	35.0	39.0	26.0	39.0
10-11	34.57797782604331	39.0	35.0	39.0	26.0	39.0
12-13	34.49659922851133	39.0	35.0	39.0	25.5	39.0
14-15	35.68626127293793	40.0	36.0	41.0	25.5	41.0
16-17	35.66341186839466	40.0	36.0	41.0	25.5	41.0
18-19	35.670210102155515	40.0	36.0	41.0	25.0	41.0
20-21	35.68825985585788	40.0	36.0	41.0	25.5	41.0
22-23	35.62686727137083	40.0	36.0	41.0	25.0	41.0
24-25	35.603356758909236	40.0	36.0	41.0	25.0	41.0
26-27	35.52478235466933	40.0	36.0	41.0	25.0	41.0
28-29	35.387563118708584	40.0	35.0	41.0	25.0	41.0
30-31	35.286701692884854	40.0	35.0	41.0	24.5	41.0
32-33	35.15469373752839	40.0	35.0	41.0	24.0	41.0
34-35	35.039350261078795	40.0	35.0	41.0	24.0	41.0
36-37	34.92633408305471	40.0	35.0	41.0	23.5	41.0
38-39	34.78948750164082	40.0	35.0	41.0	22.5	41.0
40-41	34.638477841302475	39.5	34.5	41.0	21.5	41.0
42-43	34.460925506962774	39.0	34.0	41.0	19.5	41.0
44-45	34.275848565840725	39.0	34.0	41.0	18.0	41.0
46-47	34.08881054625236	39.0	33.5	41.0	17.0	41.0
48-49	33.851370953241876	39.0	33.0	41.0	14.5	41.0
50-51	33.670498555527	38.0	33.0	40.5	13.0	41.0
52-53	33.47324406537982	38.0	33.0	40.0	10.0	41.0
54-55	33.23262045456133	38.0	33.0	40.0	8.0	41.0
56-57	32.97350475810179	37.5	32.0	40.0	7.5	41.0
58-59	32.672493829230454	37.0	32.0	40.0	4.0	41.0
60-61	32.35288620670764	36.5	31.5	40.0	2.0	41.0
62-63	32.050922223484626	36.0	31.0	39.5	2.0	41.0
64-65	31.721996031146347	35.5	31.0	39.0	2.0	41.0
66-67	31.36564985474378	35.0	31.0	39.0	2.0	40.5
68-69	30.99442911786951	35.0	30.5	38.0	2.0	40.0
70-71	30.625357165575178	35.0	30.0	37.0	2.0	40.0
72-73	30.263559606526805	35.0	30.0	37.0	2.0	39.0
74-75	30.206667556478216	35.0	30.0	36.5	2.0	39.0
76-77	30.25958707596527	35.0	31.0	36.0	2.0	39.0
78-79	30.093997191578268	35.0	31.0	36.0	2.0	37.5
80-81	29.88472234018966	35.0	31.0	35.0	2.0	37.0
82-83	29.655160520900093	35.0	31.0	35.0	2.0	37.0
84-85	29.4171210404765	35.0	30.0	35.0	2.0	36.0
86-87	29.277523948615958	35.0	30.0	35.0	2.0	36.0
88-89	29.10786427947142	35.0	30.0	35.0	2.0	36.0
90-91	28.9676884424143	35.0	30.0	35.0	2.0	35.0
92-93	28.861536682481045	35.0	30.0	35.0	2.0	35.0
94-95	28.749420979035378	35.0	30.0	35.0	2.0	35.0
96-97	28.649369575872427	34.0	29.5	35.0	2.0	35.0
98-99	28.54806140582135	34.0	29.0	35.0	2.0	35.0
100-101	28.413946582627275	34.0	29.0	35.0	2.0	35.0
102-103	27.13882199981836	32.5	26.0	34.5	2.0	35.0
104-105	28.080017958014867	34.0	29.0	35.0	2.0	35.0
106-107	28.22031678761853	34.0	29.0	35.0	2.0	35.0
108-109	28.156944556283705	34.0	29.0	35.0	2.0	35.0
110-111	28.05694132060535	34.0	29.0	35.0	2.0	35.0
112-113	27.938510161316948	34.0	29.0	35.0	2.0	35.0
114-115	27.79335965263522	34.0	29.0	35.0	2.0	35.0
116-117	27.639791048716447	34.0	29.0	35.0	2.0	35.0
118-119	27.420105402222397	34.0	27.0	35.0	2.0	35.0
120-121	27.198095398828613	34.0	27.0	35.0	2.0	35.0
122-123	26.88433369109641	34.0	26.5	35.0	2.0	35.0
124-125	25.572394442428273	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114596.0
3	15925.0
4	10890.0
5	11251.0
6	15520.0
7	15688.0
8	13860.0
9	14674.0
10	15753.0
11	16337.0
12	16357.0
13	15901.0
14	15616.0
15	14354.0
16	11756.0
17	10987.0
18	10512.0
19	10284.0
20	10859.0
21	11730.0
22	13906.0
23	17967.0
24	23916.0
25	29278.0
26	32493.0
27	36514.0
28	42370.0
29	49563.0
30	58896.0
31	71650.0
32	89112.0
33	115953.0
34	158088.0
35	241378.0
36	459208.0
37	701241.0
38	205071.0
39	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.300222966509295	17.859447754071557	11.45858303050061	25.381746248918542
2	17.156053233483757	20.806361878184692	36.46478182926076	25.572803059070797
3	19.612338459024766	24.239002996222155	28.50922675977452	27.63943178497856
4	13.666716348427387	16.170603486187552	34.53761921322492	35.62506095216014
5	14.495119281506415	37.562872237136446	33.2810824219788	14.660926059378331
6	33.75430848177239	35.1559226759602	16.489123959834384	14.600644882433029
7	28.900509953348454	32.24934962818279	20.390918832640107	18.45922158582865
8	26.86197175632486	34.39721376976715	19.555811112968076	19.185003360939916
9	26.685665542957132	15.696566764876204	18.423510501965726	39.194257190200936
10-11	25.72308933540653	26.03500715797021	26.86580650789143	21.37609699873183
12-13	25.97081317869241	23.65329705605307	26.641112357687703	23.734777407566813
14-15	23.543766148681975	24.931651048606152	23.86791758375231	27.656665218959564
16-17	23.821736556380287	27.445796449387476	24.731151529963345	24.001315464268895
18-19	24.14837254262945	26.60131283039513	25.970715538754185	23.27959908822124
20-21	25.23457543456491	25.810824089862034	25.357682683612897	23.59691779196016
22-23	24.833753059635477	25.66450940594132	25.093040148010555	24.40869738641264
24-25	24.23821720745885	25.74570071136528	25.521287596924903	24.49479448425097
26-27	24.10983326775834	25.828554456014025	25.52888471106456	24.53272756516308
28-29	24.24594507609877	25.9672279084095	25.53179156394757	24.255035451544156
30-31	24.42023417328352	25.96917753773593	25.286920418179193	24.32366787080135
32-33	24.13829780969458	25.996480024025935	25.291546316170688	24.573675850108796
34-35	24.298425849151943	25.917733293439486	25.475220731845997	24.308620125562573
36-37	24.428486940866335	25.84992291745562	25.364536582834567	24.35705355884348
38-39	24.30134761754326	25.85612251262604	25.428854724720278	24.41367514511042
40-41	24.240015869499132	25.93038250939767	25.549948806880117	24.279652814223077
42-43	24.190503842485953	25.839349551131917	25.50090682558056	24.469239780801573
44-45	24.089173148774563	25.93832410104048	25.466004328539977	24.50649842164498
46-47	24.05953109612476	25.95063335082906	25.52265954180329	24.46717601124289
48-49	23.99518868745473	25.959121137009262	25.43950232571916	24.606187849816845
50-51	23.87739028254259	26.158292509851634	25.392018571610187	24.572298635995594
52-53	23.98135918871586	25.93304337170007	25.32280281198261	24.76279462760146
54-55	23.819758323689626	25.919047323753848	25.407515265088943	24.853679087467583
56-57	23.838006934536335	25.924311933780903	25.394090977097555	24.84359015458521
58-59	23.76097751586929	25.645681036474716	25.510444369548036	25.08289707810796
60-61	23.804974556074527	25.673413931524415	25.600916776209747	24.920694736191315
62-63	23.654044568951445	25.608994240590192	25.612288065808787	25.124673124649572
64-65	23.581409922977723	25.50436335689148	25.64138466778082	25.27284205234998
66-67	23.55299204268433	25.600147187341886	25.677494135504347	25.169366634469437
68-69	23.711377385215446	25.41306689403945	25.65484060782721	25.22071511291789
70-71	23.407902784330147	25.320266782266426	25.840539305268006	25.43129112813542
72-73	23.396835015385623	25.205011332950328	25.97961816576001	25.418535485904037
74-75	23.21324570044147	25.368632824942583	26.11459318772465	25.303528286891297
76-77	23.17097722089971	25.151358899277596	26.27816839721875	25.39949548260394
78-79	23.2209483553097	25.14258557756473	26.32766633972119	25.30879972740438
80-81	23.170666630355218	25.071092541567104	26.382279326064417	25.37596150201326
82-83	23.06910926300886	25.03934828601939	26.425633533627902	25.46590891734385
84-85	23.10229340737623	24.980828922958125	26.359895082174774	25.556982587490868
86-87	22.972984410955206	24.845300047067795	26.5109626743928	25.670752867584202
88-89	23.075595700744596	24.705725441860253	26.508322103820486	25.710356753574665
90-91	23.050810530716554	24.757455381064258	26.52037073021369	25.6713633580055
92-93	23.026728624008722	24.676040231922432	26.553782592130702	25.743448551938148
94-95	22.775833520725445	24.55908261655084	26.790637436196207	25.87444642652751
96-97	22.83198296950021	24.494223043245068	26.73457277935144	25.939221207903284
98-99	22.808916782607707	24.57246550930466	26.605073489016295	26.01354421907134
100-101	22.643250292539065	24.70203707292011	26.73390234688186	25.920810287658963
102-103	22.429694897369185	24.795914819467743	26.784621998832563	25.989768284330506
104-105	22.239125427465687	24.851605843337275	26.78801528610673	26.12125344309031
106-107	22.15112868453729	24.90103907134976	26.879632008137687	26.068200235975258
108-109	22.148040911034013	25.094453889884548	26.829983734295794	25.92752146478564
110-111	22.148456821018915	25.28801198860023	26.770504569328967	25.79302662105189
112-113	22.091796735930433	25.44653592553757	26.721927053272264	25.739740285259728
114-115	21.969053198188266	25.474766292540902	26.733185410309733	25.822995098961094
116-117	21.849845060245354	25.45680133174036	26.75567874763119	25.937674860383098
118-119	21.97126699862966	25.49923240304213	26.652265330964553	25.877235267363663
120-121	21.96212160532069	25.539738228201664	26.691436225839187	25.80670394063846
122-123	22.075564119795	25.622119781749287	26.635316250816082	25.666999847639627
124-125	22.037138231145928	25.770084271549965	26.508240250942848	25.68453724636126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1962.0
1	1466.5
2	846.0
3	641.5
4	531.0
5	457.0
6	410.0
7	400.5
8	435.5
9	483.5
10	487.5
11	476.0
12	510.0
13	574.5
14	661.0
15	748.5
16	864.5
17	1083.0
18	1246.5
19	1423.0
20	1771.0
21	2272.5
22	2798.0
23	3465.0
24	4303.5
25	5296.5
26	6726.5
27	8586.5
28	10135.5
29	12041.5
30	14446.5
31	17119.0
32	20696.5
33	24084.0
34	27059.5
35	30552.0
36	35160.5
37	40532.0
38	45827.5
39	51311.0
40	57688.5
41	63762.5
42	70054.5
43	78890.0
44	87257.5
45	94242.0
46	102903.5
47	110697.5
48	117261.0
49	123435.0
50	128458.5
51	130494.5
52	127622.5
53	125901.5
54	123485.0
55	117190.5
56	109152.0
57	98961.5
58	89233.5
59	79671.0
60	68153.5
61	56026.5
62	44979.5
63	33878.0
64	24606.5
65	18676.5
66	13872.5
67	10038.0
68	7477.0
69	5628.0
70	4330.0
71	3523.5
72	2874.5
73	2430.5
74	1999.0
75	1547.5
76	1166.0
77	760.5
78	533.0
79	398.0
80	279.0
81	216.0
82	161.5
83	119.5
84	109.0
85	88.5
86	65.0
87	58.5
88	46.0
89	32.0
90	26.0
91	21.0
92	24.0
93	26.0
94	18.0
95	14.5
96	18.5
97	20.5
98	22.0
99	34.0
100	63.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29632195293779373
2	0.07313368462504923
3	0.08291425783282352
4	0.08758393000345262
5	0.09776896300553338
6	0.12953744139469503
7	0.11596965374932391
8	0.11674180426572715
9	0.12413238777987239
10-11	0.10205255991796085
12-13	0.0903599949552833
14-15	0.10063695063788825
16-17	0.10168486919586407
18-19	0.09451490011497689
20-21	0.09317282897932365
22-23	0.07475152380227505
24-25	0.08703239392030744
26-27	0.09876172795519468
28-29	0.09256613928786396
30-31	0.08725300835356552
32-33	0.09607758568388819
34-35	0.09067253206906555
36-37	0.09045191763580748
38-39	0.0800646547365735
40-41	0.09247421660733976
42-43	0.08201341556368642
44-45	0.078410046487138
46-47	0.0687765495682024
48-49	0.07043115781763791
50-51	0.07282114751126696
52-53	0.0847894805155171
54-55	0.08999230423318652
56-57	0.0991478032133963
58-59	0.06798601451569432
60-61	0.08563516917633969
62-63	0.09087476196621878
64-65	0.08149864855275094
66-67	0.07545013617425893
68-69	0.08309810319387193
70-71	0.08291425783282352
72-73	0.06315088152012169
74-75	0.09223521763797686
76-77	0.09651881455040434
78-79	0.07715989803200896
80-81	0.08986361248045263
82-83	0.09543412692021884
84-85	0.09903749599676727
86-87	0.08530424752645259
88-89	0.06487902791397654
90-91	0.08990038155266232
92-93	0.06565117843037978
94-95	0.09405528671235591
96-97	0.08087357432518641
98-99	0.07574428875193635
100-101	0.09260290836007365
102-103	0.06454810626408945
104-105	0.07041277328153307
106-107	0.08982684340824297
108-109	0.095985663003364
110-111	0.08931207639730748
112-113	0.0778952794762025
114-115	0.09184914237977525
116-117	0.08569032278465422
118-119	0.07739889700137186
120-121	0.11163090322858192
122-123	0.08953269083056555
124-125	0.11186990219794483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2719677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.3414328419013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.25047250037592	32.7841659314648
2	13.87202938226151	13.1344349476243
3	6.1152274723261595	8.685108920822362
4	3.266763318812723	6.1861302507183655
5	1.969527343776055	4.6620123237831175
6	1.2724365464127427	3.614338158454781
7	0.8467001011082503	2.805879718169309
8	0.6325689052106638	2.3957374675124536
9	0.4662739120947945	1.9866667585829674
>10	2.178252318090335	18.043435680709877
>50	0.10042647378192213	3.2009807653846893
>100	0.028541037994548	2.0949310197152973
>500	4.684126526170897E-4	0.15718610342815803
>1k	3.122751017413149E-4	0.24899195362956178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1030721662903352E-4
2	0.0	0.0	0.0	0.0	1.1030721662903352E-4
3	0.0	0.0	0.0	0.0	1.1030721662903352E-4
4	0.0	0.0	0.0	0.0	1.1030721662903352E-4
5	0.0	0.0	0.0	0.0	1.1030721662903352E-4
6	0.0	0.0	0.0	0.0	1.4707628883871136E-4
7	0.0	0.0	0.0	0.0	1.4707628883871136E-4
8	0.0	0.0	0.0	0.0	1.4707628883871136E-4
9	0.0	0.0	0.0	0.0	1.4707628883871136E-4
10-11	0.0	0.0	0.0	0.0	1.4707628883871136E-4
12-13	0.0	0.0	0.0	0.0	3.8607525820161736E-4
14-15	0.0	0.0	0.0	3.676907220967784E-5	4.2284433041129513E-4
16-17	0.0	0.0	0.0	7.353814441935568E-5	4.7799793872581195E-4
18-19	0.0	0.0	0.0	1.1030721662903352E-4	6.066896914596843E-4
20-21	0.0	0.0	0.0	2.0222989715322812E-4	6.802278358790401E-4
22-23	3.676907220967784E-5	0.0	0.0	4.0445979430645625E-4	7.721505164032346E-4
24-25	3.676907220967784E-5	0.0	0.0	9.559958774516238E-4	7.721505164032346E-4
26-27	3.676907220967784E-5	0.0	0.0	0.00204068350763712	8.456886608225903E-4
28-29	3.676907220967784E-5	0.0	0.0	0.007776658772346863	8.824577330322681E-4
30-31	3.676907220967784E-5	0.0	0.0	0.026859807249169664	9.008422691371071E-4
32-33	3.676907220967784E-5	0.0	0.0	0.07249022586137986	0.0010295340218709796
34-35	3.676907220967784E-5	0.0	0.0	0.14825289914942105	0.001139841238500013
36-37	3.676907220967784E-5	0.0	0.0	0.275290043633858	0.001139841238500013
38-39	3.676907220967784E-5	0.0	0.0	0.48163807687457	0.0011766103107096909
40-41	3.676907220967784E-5	0.0	0.0	0.7115367008655807	0.0011766103107096909
42-43	3.676907220967784E-5	0.0	0.0	0.934633046497801	0.0011766103107096909
44-45	3.676907220967784E-5	0.0	0.0	1.20012413238778	0.0012317639190242077
46-47	3.676907220967784E-5	0.0	0.0	1.5214490544281545	0.0013053020634435633
48-49	3.676907220967784E-5	0.0	0.0	1.8535473146259647	0.0013236865995484022
50-51	3.676907220967784E-5	0.0	0.0	2.206346562477824	0.0014156092800725968
52-53	3.676907220967784E-5	0.0	0.0	2.551791995887747	0.0015810701050161472
54-55	3.676907220967784E-5	0.0	0.0	2.93777533140884	0.001617839177225825
56-57	3.676907220967784E-5	0.0	0.0	3.3992823412486115	0.0016729927855403419
58-59	3.676907220967784E-5	0.0	0.0	3.9043790861929564	0.0017281463938548585
60-61	3.676907220967784E-5	0.0	0.0	4.436740098180777	0.0017649154660645363
62-63	3.676907220967784E-5	0.0	0.0	4.967023657588751	0.0019487608271129256
64-65	3.676907220967784E-5	0.0	0.0	5.525656171670386	0.0020039144354274422
66-67	3.676907220967784E-5	0.0	0.0	6.182590800304595	0.0020406835076371204
68-69	3.676907220967784E-5	0.0	0.0	6.9137621857301435	0.002059068043741959
70-71	3.676907220967784E-5	0.0	0.0	7.717534104233701	0.002059068043741959
72-73	3.676907220967784E-5	0.0	0.0	8.51602966087517	0.0022061443325806706
74-75	3.676907220967784E-5	0.0	0.0	9.310609311326308	0.0024083742297338986
76-77	3.676907220967784E-5	0.0	0.0	10.199299402098116	0.0024819123741532546
78-79	3.676907220967784E-5	0.0	0.0	11.18059240123	0.0025002969102580932
80-81	3.676907220967784E-5	0.0	0.0	12.228860265391809	0.002518681446362932
82-83	7.353814441935568E-5	0.0	0.0	13.292993984212096	0.0026106041268871265
84-85	7.353814441935568E-5	0.0	0.0	14.33661056073938	0.0026106041268871265
86-87	7.353814441935568E-5	0.0	0.0	15.435766820839387	0.002702526807411321
88-89	7.353814441935568E-5	0.0	0.0	16.632967811986497	0.002794449487935516
90-91	7.353814441935568E-5	0.0	0.0	17.88765724753344	0.002794449487935516
92-93	7.353814441935568E-5	0.0	0.0	19.151208029482913	0.0030518329934032607
94-95	7.353814441935568E-5	0.0	0.0	20.384038251601204	0.0030702175295080998
96-97	7.353814441935568E-5	0.0	0.0	21.622659602592513	0.003290831962766167
98-99	7.353814441935568E-5	0.0	0.0	22.935701555736216	0.003456292787709717
100-101	7.353814441935568E-5	0.0	0.0	24.32698809454211	0.003456292787709717
102-103	7.353814441935568E-5	0.0	0.0	25.748296580807207	0.003511446396024234
104-105	7.353814441935568E-5	0.0	0.0	27.114984610304827	0.0036033690765484285
106-107	7.353814441935568E-5	0.0	0.0	28.45455544904781	0.0036401381487581063
108-109	7.353814441935568E-5	0.0	0.0	29.807730844508374	0.0036401381487581063
110-111	7.353814441935568E-5	0.0	0.0	31.199146075067002	0.003713676293177462
112-113	7.353814441935568E-5	0.0	0.0	32.611538061321255	0.003713676293177462
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6960	0.0	32.745186	1
GTACATG	12270	0.0	29.417393	1
TACATGG	12340	0.0	29.08833	2
TCAACGC	8080	0.0	27.774525	4
CATGGGG	7910	0.0	27.543633	4
AACGCAG	8190	0.0	27.325277	6
GTATAGG	550	0.0	27.118814	1
CAACGCA	8365	0.0	27.04172	5
ACATGGG	13080	0.0	26.89654	3
GGTATCA	5305	0.0	26.87855	1
TATCAAC	8470	0.0	26.847052	2
ATCAACG	8585	0.0	26.14073	3
ACGCAGA	9455	0.0	23.41586	7
GTATACG	155	2.068981E-4	23.094732	1
CGCAGAG	9775	0.0	22.588419	8
GCAGAGT	10625	0.0	19.661058	9
GTATAGA	680	0.0	19.302214	1
ATGGGGA	4235	0.0	19.256786	5
TAATGCG	155	0.005675027	19.202375	4
ATGGGGC	3510	0.0	19.164082	5
>>END_MODULE