FastQCFastQC Report
Mon 15 Aug 2016
SRR2921094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719677
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT80260.29510857355487435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59980.22054089511364766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.19300086002859898No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT45390.1668948187597277RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.13034636098330793No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT31900.1172933403488723RNA PCR Primer, Index 31 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61400.044.891491
GTATCAA93850.042.8465651
TCAACGC115050.034.4503144
TATCAAC116500.034.073322
ATCAACG117650.033.79033
CAACGCA118250.033.669255
AACGCAG120100.033.107316
GTACATG120850.029.4232081
ACGCAGA136750.028.8852127
TACATGG120900.028.790732
CGCAGAG140650.027.95798
CATGGGG80050.027.027354
ACATGGG125250.026.8861643
GCAGAGT153650.024.9328639
GTATAGG6050.024.6533011
GAGTACT76750.023.83359512-13
AGTACTT79700.022.91410412-13
CAGAGTA145650.022.09518610-11
AGAGTAC141200.021.75937810-11
GTACTTT86450.021.40100714-15
Produced by FastQC (version 0.11.5)