##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2719677
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.460881200230762	33.0	31.0	34.0	28.0	34.0
2	31.502257804879036	33.0	31.0	34.0	28.0	34.0
3	31.60136663287589	34.0	31.0	34.0	28.0	34.0
4	35.10000525797732	37.0	35.0	37.0	32.0	37.0
5	34.8713975961116	37.0	35.0	37.0	32.0	37.0
6	34.63978001799478	37.0	35.0	37.0	31.0	37.0
7	34.5740905997293	37.0	35.0	37.0	31.0	37.0
8	34.575597763999184	37.0	35.0	37.0	31.0	37.0
9	36.15590821998347	39.0	37.0	39.0	31.0	39.0
10-11	36.12717006468047	39.0	37.0	39.0	31.0	39.0
12-13	36.08498490813432	39.0	37.0	39.0	31.0	39.0
14-15	37.35872550306526	40.0	38.0	41.0	31.0	41.0
16-17	37.34843512667129	40.0	38.0	41.0	31.0	41.0
18-19	37.360939368903	40.0	38.0	41.0	31.0	41.0
20-21	37.36396932429844	40.0	38.0	41.0	31.0	41.0
22-23	37.33027855881416	40.0	38.0	41.0	31.0	41.0
24-25	37.29011588508489	40.0	38.0	41.0	31.0	41.0
26-27	37.18093288283866	40.0	37.0	41.0	31.0	41.0
28-29	37.05878124497872	40.0	37.0	41.0	30.0	41.0
30-31	36.92430902640277	40.0	37.0	41.0	30.0	41.0
32-33	36.80388902799855	40.0	37.0	41.0	30.0	41.0
34-35	36.661139355886746	40.0	36.0	41.0	30.0	41.0
36-37	36.489192282760044	40.0	36.0	41.0	30.0	41.0
38-39	36.3452161782447	40.0	36.0	41.0	29.0	41.0
40-41	36.21353565147626	40.0	35.5	41.0	29.0	41.0
42-43	36.06356214359279	40.0	35.0	41.0	28.0	41.0
44-45	35.887143951285395	40.0	35.0	41.0	27.5	41.0
46-47	35.614573899768246	40.0	35.0	41.0	26.5	41.0
48-49	35.35492100716372	39.5	35.0	41.0	26.0	41.0
50-51	35.14049094800596	39.0	35.0	41.0	24.5	41.0
52-53	34.925806630713865	39.0	35.0	41.0	24.0	41.0
54-55	34.70876026822303	39.0	34.5	41.0	24.0	41.0
56-57	34.44708158358511	38.0	34.0	41.0	23.0	41.0
58-59	34.17979671850738	38.0	34.0	40.5	22.5	41.0
60-61	33.876045206838896	37.0	34.0	40.0	22.0	41.0
62-63	33.56333196920076	37.0	33.5	40.0	22.0	41.0
64-65	33.22605313057396	36.0	33.0	39.5	20.5	41.0
66-67	32.84307254133488	36.0	33.0	39.0	19.5	41.0
68-69	32.44405089280823	35.0	33.0	39.0	18.0	41.0
70-71	32.01671411715435	35.0	32.0	37.5	14.0	40.0
72-73	31.623294604469574	35.0	32.0	37.0	11.0	39.5
74-75	31.309080820994552	35.0	32.0	37.0	8.0	39.0
76-77	31.019535775755724	35.0	32.0	36.0	7.5	39.0
78-79	30.736219043658494	35.0	31.0	36.0	7.0	37.5
80-81	30.707176808128317	35.0	32.0	35.0	6.5	37.0
82-83	30.52720690729083	35.0	32.0	35.0	2.0	37.0
84-85	30.345512169275985	35.0	32.0	35.0	2.0	36.0
86-87	30.192703765925145	35.0	31.0	35.0	2.0	36.0
88-89	30.03554576517726	35.0	31.0	35.0	2.0	36.0
90-91	29.897298282112178	35.0	31.0	35.0	2.0	35.0
92-93	29.799789460292526	35.0	31.0	35.0	2.0	35.0
94-95	29.70348427405166	35.0	31.0	35.0	2.0	35.0
96-97	29.637832176394475	35.0	31.0	35.0	2.0	35.0
98-99	29.548714240698438	35.0	31.0	35.0	2.0	35.0
100-101	29.45391897640786	35.0	31.0	35.0	2.0	35.0
102-103	28.111559203537773	33.0	28.0	34.5	2.0	35.0
104-105	29.137290200270108	34.0	30.0	35.0	2.0	35.0
106-107	29.30177811556299	35.0	31.0	35.0	2.0	35.0
108-109	29.288361264959036	35.0	31.0	35.0	2.0	35.0
110-111	29.185568175926775	35.0	31.0	35.0	2.0	35.0
112-113	29.04932901958578	35.0	30.0	35.0	2.0	35.0
114-115	28.93831381447135	35.0	30.0	35.0	2.0	35.0
116-117	28.798184122599853	34.0	30.0	35.0	2.0	35.0
118-119	28.619998073300614	34.0	30.0	35.0	2.0	35.0
120-121	28.729797876733155	35.0	31.0	35.0	2.0	35.0
122-123	28.611428305640707	35.0	31.0	35.0	2.0	35.0
124-125	27.876557032324058	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41227.0
3	16858.0
4	7328.0
5	6615.0
6	7766.0
7	9242.0
8	10125.0
9	11308.0
10	12987.0
11	15052.0
12	15735.0
13	18584.0
14	23805.0
15	21832.0
16	13402.0
17	11834.0
18	11579.0
19	11746.0
20	13324.0
21	14363.0
22	14088.0
23	12788.0
24	15916.0
25	20366.0
26	25611.0
27	30807.0
28	37713.0
29	45095.0
30	53996.0
31	66423.0
32	84089.0
33	108431.0
34	149149.0
35	229251.0
36	458033.0
37	798267.0
38	274493.0
39	449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39660650210463	18.03938599951892	11.978468505603338	25.585538992773106
2	17.227733767948276	20.495399254672638	36.501366874780736	25.775500102598347
3	19.43808033086282	24.096464396323533	29.51846855343484	26.946986719378806
4	13.724210432647352	16.108604372988413	34.59660187552468	35.57058331883956
5	14.147878709322011	37.05713311470001	33.981856330951835	14.813131845026142
6	33.77267900156093	34.92111795619815	16.31990262003045	14.986300422210464
7	28.48756203903941	31.73420282063662	20.709979970266122	19.068255170057846
8	27.238591504235384	33.552037155869684	19.865115073243473	19.344256266651467
9	26.897908045495143	14.179558662211145	18.797288884034742	40.12524440825897
10-11	26.013449412470713	24.96405620983672	27.33022396566458	21.692270412027987
12-13	26.65440405857516	22.4368587228403	27.289475755117532	23.619261463467016
14-15	23.850712771827517	23.777441545281892	24.70064061299977	27.67120506989082
16-17	23.753648056674326	26.65576540053603	25.46023618224985	24.130350360539797
18-19	24.039600537667035	25.342799509406415	27.360099001344167	23.257500951582387
20-21	24.91120989550406	24.56608973786094	26.965601626742842	23.55709873989216
22-23	24.472680838355725	24.668378577478613	26.756581214561738	24.102359369603924
24-25	24.042094988458047	24.79616078523174	27.161061620779897	24.00068260553032
26-27	23.889531782212465	24.85709593940077	27.21496466865474	24.038407609732023
28-29	24.023920375255898	24.768981846780804	27.25849631744569	23.948601460517608
30-31	24.003524797431723	24.641088715186697	27.2618293014601	24.09355718592148
32-33	23.734928371146033	24.97125798450616	27.054254186073194	24.239559458274613
34-35	24.004173538741956	24.761004407629102	27.24589289106376	23.98892916256518
36-37	24.130435941913642	24.732564539325836	27.1407663163996	23.996233202360923
38-39	24.066733844275436	24.65982141543712	27.06306359316848	24.210381147118966
40-41	23.95762265842771	24.735661116613638	27.222519109377764	24.08419711558089
42-43	24.228249910309195	24.495602989779385	27.098999342972313	24.177147756939107
44-45	23.906188248409734	24.802629389035225	26.92837831600005	24.362804046554988
46-47	24.186251360814428	24.647107717656752	26.75212213493395	24.414518786594876
48-49	24.03888958660599	24.81934388073193	26.528991861717415	24.612774670944663
50-51	23.860780487733123	25.152154582436342	26.451288101162294	24.53577682866824
52-53	23.967881579090115	25.008076677039014	26.50922616699939	24.514815576871484
54-55	23.697182159777213	25.10088932651881	26.418045063010435	24.78388345069354
56-57	23.83153950183367	25.218509730084058	26.129470290307427	24.820480477774844
58-59	23.44058013704688	25.181715731890897	26.49213204115709	24.885572089905136
60-61	23.669567682932545	25.445739117419542	26.26499993840144	24.61969326124647
62-63	23.28534940426293	25.561173671619148	26.2830011710827	24.870475753035226
64-65	23.191334499953292	25.43437468508249	26.259945876483926	25.114344938480286
66-67	23.190653436391298	25.508501473088508	26.235373468666484	25.065471621853714
68-69	23.401649698822762	25.26523786915312	26.145563910659277	25.187548521364846
70-71	23.22257660191718	25.107484685501095	26.207028192800863	25.462910519780863
72-73	23.21180367182026	25.002216038972865	26.20671137977391	25.57926890943297
74-75	23.057879140252822	25.042548997542674	26.27477826345663	25.624793598747875
76-77	23.04676267193835	24.82391556478297	26.293504457177484	25.835817306101195
78-79	23.021055989644385	24.858718106422707	26.223463964498183	25.896761939434725
80-81	22.83981376944039	24.815479495882993	26.259501840608117	26.085204894068497
82-83	22.6836459144298	24.767720110133673	26.26558293288458	26.283051042551946
84-85	22.622390159141684	24.681689053148357	26.185876750236737	26.510044037473225
86-87	22.50441780979966	24.571388331319095	26.292123179808126	26.632070679073124
88-89	22.466593799579716	24.556839637733873	26.231985807099832	26.74458075558658
90-91	22.42244966369525	24.46857707942323	26.19142754328456	26.917545713596958
92-93	22.31253923417826	24.475691633976883	26.138687556748202	27.07308157509665
94-95	22.07228716289204	24.26085157826513	26.380741809891106	27.28611944895173
96-97	22.121813643238372	24.06902987647624	26.419153308294526	27.390003171990863
98-99	22.05643408435102	24.21651405298731	26.167330987920117	27.559720874741544
100-101	21.98837466332756	24.169596478561648	26.242814620236427	27.599214237874364
102-103	21.74236918871924	24.123149944761295	26.257452811730907	27.877028054788553
104-105	21.594223364183197	24.22942456861125	26.152769518909103	28.023582548296456
106-107	21.418633978032116	24.262499581670895	26.244478515536652	28.07438792476034
108-109	21.377167999382213	24.413994340632165	26.1945991464982	28.014238513487427
110-111	21.324672368109166	24.59143820042482	26.039736015133396	28.04415341633262
112-113	21.153535684142977	24.713969708058052	25.949735308665584	28.182759299133387
114-115	21.0429329183264	24.82155412839718	25.866101425654726	28.269411527621692
116-117	20.839593221136777	24.811899986191627	25.974634882524946	28.373871910146654
118-119	20.88156256458041	24.885842062336962	25.883110655794972	28.349484717287655
120-121	20.813354069298303	24.950006260133648	25.842217789773862	28.394421880794184
122-123	20.723505194973292	25.01935980632104	25.772167621725934	28.484967376979736
124-125	20.54800363355111	25.22843443107734	25.740282723516067	28.483279211855482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	84.0
1	82.0
2	178.0
3	337.0
4	290.0
5	217.5
6	360.0
7	689.0
8	1104.5
9	1294.5
10	1056.5
11	813.0
12	823.0
13	935.0
14	1080.5
15	1221.5
16	1351.0
17	1520.5
18	1788.5
19	1973.0
20	2187.0
21	2578.0
22	3026.5
23	3588.0
24	4216.0
25	4996.0
26	6367.0
27	8179.5
28	9735.5
29	11692.5
30	14151.0
31	16951.5
32	20540.0
33	23765.5
34	26717.0
35	29996.0
36	34454.5
37	39807.0
38	45018.0
39	50514.0
40	56875.0
41	62979.5
42	69181.0
43	78077.5
44	86188.0
45	92914.5
46	102228.5
47	110612.0
48	116819.0
49	122486.0
50	128513.0
51	131205.5
52	128586.5
53	127453.0
54	125628.0
55	119893.0
56	111697.0
57	101584.0
58	92025.5
59	82419.5
60	69968.5
61	57082.5
62	45577.5
63	33599.5
64	23850.0
65	17738.5
66	12739.0
67	8962.5
68	6577.5
69	4768.5
70	3608.0
71	2859.0
72	2345.0
73	2047.5
74	1539.5
75	1132.0
76	875.0
77	523.0
78	289.0
79	174.5
80	100.0
81	72.5
82	52.5
83	29.5
84	26.0
85	16.0
86	11.5
87	10.5
88	9.0
89	7.5
90	4.5
91	3.5
92	6.0
93	6.5
94	7.0
95	16.5
96	18.5
97	9.0
98	4.5
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18222752187116337
2	0.012317639190242077
3	0.0
4	0.004338750520741986
5	0.14777490121069523
6	0.07629582483508152
7	0.008089195886129125
8	0.011839641251516264
9	0.013016251562225956
10-11	0.00562566804808071
12-13	0.010993952590693674
14-15	0.01207864022087917
16-17	0.015479779400274372
18-19	0.019193455693451832
20-21	0.02294390105883897
22-23	0.025297121680258355
24-25	0.025131660855314805
26-27	0.023256438172621234
28-29	0.021050293840040563
30-31	0.024102126833443826
32-33	0.024304356730597054
34-35	0.023587359822508334
36-37	0.0241388959056535
38-39	0.019009610332403445
40-41	0.026620808279806753
42-43	0.022557825800637355
44-45	0.032007477358524564
46-47	0.02636342477433901
48-49	0.01807199899105666
50-51	0.014817936100500171
52-53	0.015718778369637275
54-55	0.017814615485588915
56-57	0.014873089708814687
58-59	0.01428478455345984
60-61	0.01682185053592761
62-63	0.014652475275556619
64-65	0.026142810341080944
66-67	0.02059068043741959
68-69	0.01889930311577441
70-71	0.02518681446362932
72-73	0.0316949402447423
74-75	0.01687700414424213
76-77	0.008934884546951715
78-79	0.01395386290357274
80-81	0.017171156721919552
82-83	0.016031315483419538
84-85	0.014781167028290491
86-87	0.010534339188072702
88-89	0.020995140231726047
90-91	0.01807199899105666
92-93	0.015737162905742116
94-95	0.011563873209943681
96-97	0.02075614126236314
98-99	0.022465903120113158
100-101	0.028974028901226138
102-103	0.02092160208730669
104-105	0.013898709295258225
106-107	0.019340531982290545
108-109	0.01073656908522593
110-111	0.024175664977863182
112-113	0.017355002082967943
114-115	0.013420711356532411
116-117	0.011490335065524325
118-119	0.00731704536972589
120-121	0.003180524746137133
122-123	0.0045961340262097305
124-125	8.640731969274293E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2719677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.921356998962565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5624857034901	29.235146973213304
2	14.840447684651945	13.036252015640512
3	6.560703647243092	8.64465021164879
4	3.6128360080814574	6.347226403586101
5	2.204622193831311	4.841499921155054
6	1.4369808740295524	3.7868489981359943
7	0.947307606521995	2.9124924901719718
8	0.7097563114826857	2.493876827111833
9	0.49744483218548	1.9663606855536742
>10	2.4696522939477252	19.001363415073026
>50	0.11987298129414704	3.555036594312553
>100	0.03629533404722481	2.6226501996254306
>500	8.392258911240538E-4	0.25289604811117244
>1k	5.035355346642278E-4	0.5932975936941053
>5k	2.517677673321139E-4	0.7104016229663574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	8026	0.29510857355487435	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5998	0.22054089511364766	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5249	0.19300086002859898	No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT	4539	0.1668948187597277	RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3545	0.13034636098330793	No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT	3190	0.1172933403488723	RNA PCR Primer, Index 31 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.353814441935568E-5
2	0.0	0.0	0.0	0.0	7.353814441935568E-5
3	0.0	0.0	0.0	0.0	7.353814441935568E-5
4	0.0	0.0	0.0	0.0	7.353814441935568E-5
5	0.0	0.0	0.0	0.0	7.353814441935568E-5
6	0.0	0.0	0.0	0.0	7.353814441935568E-5
7	0.0	0.0	0.0	0.0	7.353814441935568E-5
8	0.0	0.0	0.0	0.0	7.353814441935568E-5
9	0.0	0.0	0.0	0.0	7.353814441935568E-5
10-11	0.0	0.0	0.0	0.0	1.1030721662903352E-4
12-13	0.0	0.0	0.0	0.0	2.0222989715322812E-4
14-15	0.0	0.0	0.0	3.676907220967784E-5	2.573835054677449E-4
16-17	0.0	0.0	0.0	7.353814441935568E-5	3.676907220967784E-4
18-19	0.0	0.0	0.0	1.1030721662903352E-4	4.0445979430645625E-4
20-21	0.0	0.0	0.0	2.0222989715322812E-4	6.434587636693622E-4
22-23	0.0	0.0	0.0	3.6769072209677837E-4	6.98612371983879E-4
24-25	0.0	0.0	0.0	9.19226805241946E-4	6.98612371983879E-4
26-27	0.0	0.0	0.0	0.0019671453632177645	7.721505164032346E-4
28-29	0.0	0.0	0.0	0.0076847360918226686	8.089195886129125E-4
30-31	0.0	0.0	0.0	0.026675961888121272	8.456886608225903E-4
32-33	0.0	0.0	0.0	0.07247184132527502	9.19226805241946E-4
34-35	0.0	0.0	0.0	0.1478484393551146	0.0010663030940806573
36-37	0.0	0.0	0.0	0.27488558383955153	0.0010663030940806573
38-39	0.0	0.0	0.0	0.4803695438833361	0.0010663030940806573
40-41	0.0	0.0	0.0	0.7098820926161452	0.0010663030940806573
42-43	0.0	0.0	0.0	0.933254206289938	0.0010846876301854962
44-45	0.0	0.0	0.0	1.1982305251689815	0.00119499484681453
46-47	0.0	0.0	0.0	1.5195002936010416	0.001342071135653241
48-49	0.0	0.0	0.0	1.8529957785428195	0.0015075319605967915
50-51	0.0	0.0	0.0	2.2061259480445656	0.0015443010328064692
52-53	0.0	0.0	0.0	2.5526744536207793	0.001617839177225825
54-55	0.0	0.0	0.0	2.9412500087326547	0.0016546082494355028
56-57	0.0	0.0	0.0	3.40687515465991	0.0016913773216451807
58-59	0.0	0.0	0.0	3.915575268680803	0.0017097618577500196
60-61	0.0	0.0	0.0	4.452256646653261	0.0017281463938548585
62-63	0.0	0.0	0.0	4.985739115343476	0.0019119917549032478
64-65	0.0	0.0	0.0	5.549372223245628	0.0019303762910080867
66-67	0.0	0.0	0.0	6.211325830236458	0.0019487608271129256
68-69	0.0	0.0	0.0	6.946210891955184	0.0019487608271129256
70-71	0.0	0.0	0.0	7.752850798091097	0.0019671453632177645
72-73	0.0	0.0	0.0	8.552136889785075	0.002059068043741959
74-75	0.0	0.0	0.0	9.348628531991116	0.0022061443325806706
76-77	0.0	0.0	0.0	10.238862923795729	0.002316451549209704
78-79	0.0	0.0	0.0	11.223373216745959	0.0023532206214193817
80-81	0.0	0.0	0.0	12.277505747925213	0.0023899896936290595
82-83	0.0	0.0	0.0	13.348625590465339	0.002537065982467771
84-85	0.0	0.0	0.0	14.400956437106318	0.002537065982467771
86-87	0.0	0.0	0.0	15.50717235907058	0.0026289886629919657
88-89	0.0	0.0	0.0	16.712462546103822	0.0027209113435161603
90-91	0.0	0.0	0.0	17.97435871980386	0.0027209113435161603
92-93	0.0	0.0	0.0	19.24465662650381	0.0028679876323548714
94-95	0.0	0.0	0.0	20.483480207392276	0.0029231412406693887
96-97	0.0	0.0	0.0	21.72881191406185	0.0031621402100322944
98-99	0.0	0.0	0.0	23.050476214638724	0.0033827546432903615
100-101	0.0	0.0	0.0	24.454153930779277	0.0033827546432903615
102-103	0.0	0.0	0.0	25.885518758293724	0.0034379082516048784
104-105	0.0	0.0	0.0	27.26222635996848	0.003493061859919395
106-107	0.0	0.0	0.0	28.610879159547256	0.003493061859919395
108-109	0.0	0.0	0.0	29.97594934986765	0.0035298309321290726
110-111	0.0	0.0	0.0	31.3807301381745	0.0035298309321290726
112-113	0.0	0.0	0.0	32.8066163739297	0.0035298309321290726
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6140	0.0	44.89149	1
GTATCAA	9385	0.0	42.846565	1
TCAACGC	11505	0.0	34.450314	4
TATCAAC	11650	0.0	34.07332	2
ATCAACG	11765	0.0	33.7903	3
CAACGCA	11825	0.0	33.66925	5
AACGCAG	12010	0.0	33.10731	6
GTACATG	12085	0.0	29.423208	1
ACGCAGA	13675	0.0	28.885212	7
TACATGG	12090	0.0	28.79073	2
CGCAGAG	14065	0.0	27.9579	8
CATGGGG	8005	0.0	27.02735	4
ACATGGG	12525	0.0	26.886164	3
GCAGAGT	15365	0.0	24.932863	9
GTATAGG	605	0.0	24.653301	1
GAGTACT	7675	0.0	23.833595	12-13
AGTACTT	7970	0.0	22.914104	12-13
CAGAGTA	14565	0.0	22.095186	10-11
AGAGTAC	14120	0.0	21.759378	10-11
GTACTTT	8645	0.0	21.401007	14-15
>>END_MODULE