FastQCFastQC Report
Mon 15 Aug 2016
SRR2921093_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921093_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654355
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG208100.033.253171
GTATCAA121200.033.0760651
TACATGG207650.032.32912
GGTATCA87700.032.174271
CATGGGG119850.031.5830044
ACATGGG217400.030.5519563
GTATAGG8050.028.9012011
AACGCAG136300.028.423136
TCAACGC138350.028.263154
CAACGCA143450.027.3413055
ATCAACG145000.027.007993
TATCAAC152700.026.3465752
ACGCAGA155200.024.9214157
CGCAGAG159300.024.093568
ATGGGGA50550.023.6651525
GCAGAGT170450.021.854179
ATGGGGC52250.021.6422125
CATGGGA106500.020.5651844
ATGGGGG57900.020.5582335
TACCGTA4152.910383E-1120.0738667
Produced by FastQC (version 0.11.5)