##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921093_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3654355
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.9346535845587	31.0	31.0	34.0	26.0	34.0
2	30.10293526490995	31.0	31.0	34.0	26.0	34.0
3	30.29282540968242	31.0	31.0	34.0	26.0	34.0
4	33.64209798993256	37.0	35.0	37.0	28.0	37.0
5	33.81180290365879	37.0	35.0	37.0	28.0	37.0
6	33.77886932167236	37.0	35.0	37.0	29.0	37.0
7	33.663073784566635	37.0	35.0	37.0	28.0	37.0
8	33.653939204045585	37.0	35.0	37.0	28.0	37.0
9	35.103215752164196	39.0	35.0	39.0	27.0	39.0
10-11	35.09416381276586	39.0	35.0	39.0	27.0	39.0
12-13	35.0249428421705	39.0	35.0	39.0	27.0	39.0
14-15	36.27208125647344	40.0	37.0	41.0	27.0	41.0
16-17	36.27261212991075	40.0	37.0	41.0	27.0	41.0
18-19	36.309610040622765	40.0	37.0	41.0	27.0	41.0
20-21	36.35329640935267	40.0	37.0	41.0	27.0	41.0
22-23	36.31665300716543	40.0	37.0	41.0	27.0	41.0
24-25	36.31230613883982	40.0	36.5	41.0	27.0	41.0
26-27	36.25688869855282	40.0	36.0	41.0	28.0	41.0
28-29	36.128242466864876	40.0	36.0	41.0	27.0	41.0
30-31	36.01821580005227	40.0	36.0	41.0	27.0	41.0
32-33	35.877509300546876	40.0	36.0	41.0	27.0	41.0
34-35	35.74995026482102	40.0	35.5	41.0	27.0	41.0
36-37	35.62505120602678	40.0	35.0	41.0	26.0	41.0
38-39	35.48022482763716	40.0	35.0	41.0	25.5	41.0
40-41	35.32365068527825	40.0	35.0	41.0	25.0	41.0
42-43	35.150106379922036	40.0	35.0	41.0	24.0	41.0
44-45	34.96238665920525	39.5	35.0	41.0	24.0	41.0
46-47	34.75892093132714	39.0	34.5	41.0	23.0	41.0
48-49	34.51510417022976	39.0	34.0	41.0	22.5	41.0
50-51	34.32932049568255	39.0	34.0	41.0	21.5	41.0
52-53	34.13392951697358	38.5	34.0	41.0	20.5	41.0
54-55	33.89256065707902	38.0	33.0	40.0	19.5	41.0
56-57	33.637371711286946	38.0	33.0	40.0	18.0	41.0
58-59	33.33831483257647	37.5	33.0	40.0	15.5	41.0
60-61	33.03083430591719	37.0	32.5	40.0	13.0	41.0
62-63	32.730711986109725	36.5	32.0	40.0	10.5	41.0
64-65	32.395838663731354	36.0	32.0	39.0	8.0	41.0
66-67	32.03991183122603	35.5	31.5	39.0	7.0	41.0
68-69	31.666961611556623	35.0	31.0	38.5	6.0	40.0
70-71	31.296641951862913	35.0	31.0	37.5	2.0	40.0
72-73	30.939991599064676	35.0	31.0	37.0	2.0	39.5
74-75	30.874187510518272	35.0	31.0	37.0	2.0	39.0
76-77	30.91480001258772	35.0	31.5	36.0	2.0	39.0
78-79	30.73367913079052	35.0	32.0	36.0	2.0	38.5
80-81	30.513883161323953	35.0	32.0	35.5	2.0	37.0
82-83	30.268969216181787	35.0	31.5	35.0	2.0	37.0
84-85	30.01596273487387	35.0	31.0	35.0	2.0	36.5
86-87	29.864739057918566	35.0	31.0	35.0	2.0	36.0
88-89	29.684898018939048	35.0	31.0	35.0	2.0	36.0
90-91	29.525218814264075	35.0	31.0	35.0	2.0	35.5
92-93	29.396972242707673	35.0	31.0	35.0	2.0	35.0
94-95	29.262546194882546	35.0	31.0	35.0	2.0	35.0
96-97	29.14715414895378	35.0	30.5	35.0	2.0	35.0
98-99	29.025842727375966	35.0	30.0	35.0	2.0	35.0
100-101	28.862525808247966	35.0	30.0	35.0	2.0	35.0
102-103	27.573337976195525	33.0	27.5	34.5	2.0	35.0
104-105	28.478950320918464	34.0	29.0	35.0	2.0	35.0
106-107	28.5785228857076	35.0	29.5	35.0	2.0	35.0
108-109	28.482756738193196	35.0	29.0	35.0	2.0	35.0
110-111	28.347650269336174	35.0	29.0	35.0	2.0	35.0
112-113	28.194605067104867	35.0	29.0	35.0	2.0	35.0
114-115	28.004334280604922	34.0	29.0	35.0	2.0	35.0
116-117	27.818059000835987	34.0	29.0	35.0	2.0	35.0
118-119	27.55945645674818	34.0	28.5	35.0	2.0	35.0
120-121	27.291375906281683	34.0	27.0	35.0	2.0	35.0
122-123	26.9266101678682	34.0	26.0	35.0	2.0	35.0
124-125	25.609082177292574	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116679.0
3	17033.0
4	12118.0
5	13516.0
6	20946.0
7	21297.0
8	17663.0
9	18058.0
10	19863.0
11	21037.0
12	21557.0
13	21454.0
14	22844.0
15	20247.0
16	15388.0
17	13824.0
18	12997.0
19	12923.0
20	13631.0
21	14524.0
22	17381.0
23	22718.0
24	30974.0
25	39712.0
26	44025.0
27	46199.0
28	53929.0
29	63386.0
30	76075.0
31	93303.0
32	117992.0
33	154438.0
34	213129.0
35	326269.0
36	614033.0
37	966186.0
38	326429.0
39	578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81971467159733	17.80658147415558	11.412998879402215	24.960704974844873
2	16.755007872937632	20.919203121790922	37.19616622167454	25.129622783596904
3	19.11987182998302	25.24954811852988	28.67500136933779	26.955578682149312
4	13.045050108169887	16.42193897295712	35.06703684623224	35.465974072640755
5	13.915388146714887	38.51479496750881	33.12430358416538	14.445513301610918
6	32.77650549292772	35.971182611145	16.848111109101758	14.404200786825522
7	28.824338740883082	32.967615522062246	20.46992368938561	17.738122047669066
8	26.771441487102916	34.72579827673594	19.61121080533143	18.891549430829716
9	27.018251999018027	15.954936610086273	18.42477598723434	38.60203540366136
10-11	25.370809843630866	26.55866140163142	26.729645845525884	21.340882909211835
12-13	25.79155671016539	24.337104379133212	26.67718871069126	23.19415020001013
14-15	23.49506193857106	25.505225268707264	24.052452239292286	26.947260553429388
16-17	23.467054766030294	28.07729609135346	24.94036957917872	23.51527956343752
18-19	23.702885131328216	27.00420253576456	26.37413716268166	22.91877517022557
20-21	24.87264825884601	26.168502554978048	25.82158923578547	23.13725995039048
22-23	24.469570177707496	26.044281252237894	25.626121001245895	23.860027568808714
24-25	23.914452818158214	26.309903733332458	25.91800899891211	23.857634449597217
26-27	23.667245372906688	26.41243858664925	25.946455885382196	23.973860155061864
28-29	23.78303358885636	26.449810364062103	26.05891135523069	23.708244691850844
30-31	23.898406260745816	26.47510134189945	25.827425231719324	23.79906716563541
32-33	23.581263882356776	26.64360667224175	25.747781219672465	24.027348225729003
34-35	23.7688547986208	26.532291981794927	25.987887297055117	23.710965922529155
36-37	23.992524752922705	26.38881090380317	25.72342845748665	23.89523588578747
38-39	23.806599272821284	26.451796749276713	25.830016771777842	23.911587206124164
40-41	23.744427438095123	26.510425853838644	25.92929899389853	23.815847714167703
42-43	23.73292675979808	26.4946479746547	25.854784889223865	23.917640376323355
44-45	23.600283448070464	26.448320364785342	25.864458419058117	24.086937768086074
46-47	23.67477227659872	26.570767419886916	25.872491872962595	23.881968430551773
48-49	23.553040177886828	26.590465967095316	25.68086347405087	24.175630380966986
50-51	23.513895972752323	26.687442449552435	25.71957485409143	24.07908672360381
52-53	23.590808993044615	26.496936143861767	25.619238280460984	24.29301658263264
54-55	23.444690252761372	26.439478246055252	25.694434363473167	24.42139713771021
56-57	23.574712015546716	26.47120415693045	25.5559075453043	24.398176282218532
58-59	23.34744588469568	26.167633122224682	25.8231621827072	24.66175881037244
60-61	23.36475602564977	26.302940718117934	25.794845122799927	24.53745813343237
62-63	23.275269168857186	26.18802282467548	25.917998028464282	24.61870997800305
64-65	23.253687282106743	26.176289790445896	25.907289745119584	24.662733182327777
66-67	23.33567207644787	26.263222525108628	25.867334112184086	24.533771286259416
68-69	23.57991989858806	25.941332638045665	25.97082941400971	24.50791804935657
70-71	23.28461713742344	25.862080298327932	26.206205816912625	24.647096747336008
72-73	23.380750273822564	25.94819277108434	26.074055312157725	24.59700164293538
74-75	23.272007451302148	26.030720324040157	26.174465485343294	24.522806739314397
76-77	23.180338160929804	25.746552691818962	26.428002269124644	24.645106878126587
78-79	23.319693619240144	25.78385222716498	26.3824468477691	24.51400730582578
80-81	23.31331234548762	25.79300148288059	26.33301762091612	24.56066855071567
82-83	23.247832953784105	25.675080844807006	26.406244949767476	24.670841251641413
84-85	23.13555824882032	25.61463709200817	26.364636304481603	24.885168354689913
86-87	23.129603376932693	25.543736668488936	26.463398668464833	24.863261286113534
88-89	23.322901849996665	25.410225372888906	26.381767645588607	24.88510513152582
90-91	23.28142200057026	25.470443600041527	26.4163979480516	24.831736451336617
92-93	23.261544940755034	25.445906645926854	26.417301505087675	24.875246908230437
94-95	23.116092262880567	25.3044582477021	26.63705969246985	24.942389796947488
96-97	23.255877326929035	25.22545061708869	26.491151024765575	25.027521031216704
98-99	23.07944257254744	25.52365457222045	26.356950364156123	25.03995249107599
100-101	23.03144082328768	25.665439882965025	26.48718820655161	24.815931087195683
102-103	22.99556080457285	25.764023417395954	26.39110786287852	24.84930791515267
104-105	22.742407187086535	26.030336062183217	26.346329293207845	24.880927457522407
106-107	22.7563056559138	26.042095636243566	26.323556741487863	24.87804196635477
108-109	22.750311788067933	26.156026336744258	26.310721975984443	24.782939899203363
110-111	22.81730111208209	26.426202863396032	26.254000848273645	24.502495176248228
112-113	22.82121834798667	26.593059021133612	26.237317490586452	24.348405140293263
114-115	22.786683610505893	26.778127327774953	26.114630577877772	24.32055848384138
116-117	22.721972335753378	26.670875441048285	26.14469191155274	24.462460311645593
118-119	22.86260924910233	26.661245006371527	26.02821436034313	24.44793138418301
120-121	22.94013413596108	26.86171108962661	26.019957388367743	24.178197386044566
122-123	23.059891831841405	26.928289430076617	25.939596524989717	24.07222221309226
124-125	23.195401306926325	27.259365494871123	25.681629678638036	23.863603519564514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2856.0
1	2192.5
2	1376.0
3	1135.5
4	907.0
5	734.0
6	686.5
7	699.0
8	787.5
9	875.0
10	885.0
11	913.5
12	991.0
13	1056.5
14	1203.0
15	1434.5
16	1659.5
17	1967.0
18	2310.0
19	2717.0
20	3313.5
21	4140.0
22	5156.0
23	6320.5
24	7827.0
25	9928.5
26	12797.5
27	16135.0
28	18926.5
29	22058.5
30	26209.0
31	31351.5
32	37649.0
33	42659.0
34	47008.5
35	52860.0
36	61367.0
37	69212.0
38	74947.0
39	81162.0
40	87236.5
41	92712.0
42	98204.0
43	107326.0
44	114892.5
45	120201.0
46	128561.5
47	136436.0
48	143870.0
49	151207.0
50	158572.5
51	160355.0
52	156365.5
53	154696.0
54	151053.5
55	144175.0
56	134940.0
57	122825.0
58	112683.5
59	102096.0
60	88378.5
61	74038.5
62	61173.0
63	47571.0
64	34950.0
65	26804.5
66	20172.5
67	14750.0
68	10928.5
69	8339.5
70	6697.5
71	5333.0
72	4354.5
73	3906.0
74	3292.0
75	2585.5
76	1804.0
77	1100.5
78	769.5
79	531.5
80	331.0
81	221.0
82	170.5
83	136.5
84	110.5
85	92.0
86	72.5
87	58.5
88	48.5
89	29.5
90	26.5
91	23.5
92	18.0
93	16.5
94	13.0
95	11.0
96	12.0
97	10.0
98	11.5
99	19.5
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29455266387638857
2	0.07128480949442514
3	0.08086242305413678
4	0.0863079804780871
5	0.09629606319035781
6	0.13006399214088396
7	0.11692897925899373
8	0.11780464678445307
9	0.12486471620846908
10-11	0.1020836782414407
12-13	0.09002956746128934
14-15	0.10074281234308106
16-17	0.10243941817365855
18-19	0.09479100963097455
20-21	0.09421635281739185
22-23	0.07564946481663659
24-25	0.08885288922395335
26-27	0.10078385925833698
28-29	0.09336804990210311
30-31	0.08823718549511474
32-33	0.09818422129212953
34-35	0.09288916922411752
36-37	0.09227346549527891
38-39	0.0816286321389137
40-41	0.0938879774953446
42-43	0.08283267498642032
44-45	0.07915213491847398
46-47	0.06845257234176756
48-49	0.0710111633927191
50-51	0.07299509763008794
52-53	0.08634902739334302
54-55	0.09199981939357287
56-57	0.10071544773291045
58-59	0.06904091146043556
60-61	0.08680054346115798
62-63	0.09287548691903222
64-65	0.08358520176611194
66-67	0.07719556529127576
68-69	0.0850628907153246
70-71	0.08403671783392692
72-73	0.06444365695177398
74-75	0.09439422278350078
76-77	0.09873151349554163
78-79	0.07896058264727975
80-81	0.09175353790203744
82-83	0.09667916773274628
84-85	0.10082490617359288
86-87	0.08841505546122366
88-89	0.06648232040948403
90-91	0.09265657003766738
92-93	0.06681069573153128
94-95	0.09648761546155205
96-97	0.08363993098645316
98-99	0.07745552908789649
100-101	0.09524252569878953
102-103	0.06716643566374915
104-105	0.07373394210469426
106-107	0.09321754454616478
108-109	0.09955245180065977
110-111	0.0933270029868472
112-113	0.08121816298635463
114-115	0.09496887959708347
116-117	0.08900339457989166
118-119	0.08078032922362496
120-121	0.11432934129278628
122-123	0.09230083010544952
124-125	0.11458930508940703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3654355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.8849434010733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85062816934885	24.94747321120905
2	13.429213083325847	10.175299551655057
3	6.314695866657178	7.176956865098962
4	3.7628616703947833	5.70223205635898
5	2.432544515869478	4.607840565215322
6	1.6434074105121543	3.735623803929445
7	1.2266241955400923	3.2529411755696422
8	0.8783979050853594	2.6622443934224163
9	0.662967738238868	2.2604845715925386
>10	3.5187440383035034	23.868054385349453
>50	0.19481856849528165	5.05908246003661
>100	0.08118222459668588	5.167532820159417
>500	0.003262178028902477	0.8272942556087041
>1k	6.524356028711465E-4	0.5569398847944623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.7364610170604663E-5	4.104691525590699E-5
14-15	0.0	0.0	0.0	5.4729220341209326E-5	8.209383051181399E-5
16-17	0.0	0.0	0.0	5.4729220341209326E-5	8.209383051181399E-5
18-19	0.0	0.0	0.0	6.841152542651166E-5	8.209383051181399E-5
20-21	0.0	0.0	0.0	9.577613559711633E-5	8.209383051181399E-5
22-23	0.0	0.0	0.0	4.378337627296746E-4	8.209383051181399E-5
24-25	0.0	0.0	0.0	0.0010809021017388841	8.209383051181399E-5
26-27	0.0	0.0	0.0	0.002763825627231071	8.209383051181399E-5
28-29	0.0	0.0	0.0	0.00838725301729033	8.209383051181399E-5
30-31	0.0	0.0	0.0	0.027788761628249038	8.209383051181399E-5
32-33	0.0	0.0	0.0	0.07053228271473352	8.209383051181399E-5
34-35	0.0	0.0	0.0	0.1403804501752019	8.209383051181399E-5
36-37	0.0	0.0	0.0	0.2558591050951536	8.209383051181399E-5
38-39	0.0	0.0	0.0	0.4436076954756722	9.577613559711633E-5
40-41	0.0	0.0	0.0	0.6613889455184294	1.0945844068241865E-4
42-43	0.0	0.0	0.0	0.8619304911537057	1.0945844068241865E-4
44-45	0.0	0.0	0.0	1.1049829586890163	1.0945844068241865E-4
46-47	0.0	0.0	0.0	1.4001239616840728	1.0945844068241865E-4
48-49	0.0	0.0	0.0	1.7246819206125295	1.0945844068241865E-4
50-51	0.0	0.0	0.0	2.0774117457116237	1.0945844068241865E-4
52-53	0.0	0.0	0.0	2.417129698674595	1.0945844068241865E-4
54-55	0.0	0.0	0.0	2.787044498960829	1.0945844068241865E-4
56-57	0.0	0.0	0.0	3.2272726650804318	1.0945844068241865E-4
58-59	0.0	0.0	0.0	3.7157172743206393	1.0945844068241865E-4
60-61	0.0	0.0	0.0	4.252419373596709	1.0945844068241865E-4
62-63	0.0	0.0	0.0	4.781582522770776	1.0945844068241865E-4
64-65	0.0	0.0	0.0	5.318202528216334	1.0945844068241865E-4
66-67	0.0	0.0	0.0	5.942662932309531	1.0945844068241865E-4
68-69	0.0	0.0	0.0	6.65016124596543	1.0945844068241865E-4
70-71	0.0	0.0	0.0	7.436209673116049	1.0945844068241865E-4
72-73	0.0	0.0	0.0	8.218659654029235	1.0945844068241865E-4
74-75	0.0	0.0	0.0	8.983213179890843	1.0945844068241865E-4
76-77	0.0	0.0	0.0	9.820392381145236	1.0945844068241865E-4
78-79	0.0	0.0	0.0	10.76140659569199	1.0945844068241865E-4
80-81	0.0	0.0	0.0	11.776565221495996	1.0945844068241865E-4
82-83	0.0	0.0	0.0	12.824397191843705	1.0945844068241865E-4
84-85	0.0	0.0	0.0	13.823068640019923	1.0945844068241865E-4
86-87	0.0	0.0	0.0	14.853715635180489	1.2314074576772098E-4
88-89	0.0	0.0	0.0	15.969192921869933	1.3682305085302332E-4
90-91	0.0	0.0	0.0	17.167502883545797	1.5050535593832563E-4
92-93	0.0	0.0	0.0	18.400579582443413	1.6418766102362797E-4
94-95	0.0	0.0	0.0	19.589804493542637	1.6418766102362797E-4
96-97	0.0	0.0	0.0	20.774377420912856	1.9155227119423262E-4
98-99	0.0	0.0	0.0	22.0046355649629	1.9155227119423262E-4
100-101	0.0	0.0	0.0	23.332530638101662	1.9155227119423262E-4
102-103	0.0	0.0	0.0	24.69460410934351	2.0523457627953496E-4
104-105	0.0	0.0	0.0	25.99944449841354	2.189168813648373E-4
106-107	0.0	0.0	0.0	27.25827129548169	2.189168813648373E-4
108-109	0.0	0.0	0.0	28.53153292441484	2.189168813648373E-4
110-111	0.0	0.0	0.0	29.865653446367418	2.189168813648373E-4
112-113	0.0	0.0	0.0	31.24090297740641	2.189168813648373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	20810	0.0	33.25317	1
GTATCAA	12120	0.0	33.076065	1
TACATGG	20765	0.0	32.3291	2
GGTATCA	8770	0.0	32.17427	1
CATGGGG	11985	0.0	31.583004	4
ACATGGG	21740	0.0	30.551956	3
GTATAGG	805	0.0	28.901201	1
AACGCAG	13630	0.0	28.42313	6
TCAACGC	13835	0.0	28.26315	4
CAACGCA	14345	0.0	27.341305	5
ATCAACG	14500	0.0	27.00799	3
TATCAAC	15270	0.0	26.346575	2
ACGCAGA	15520	0.0	24.921415	7
CGCAGAG	15930	0.0	24.09356	8
ATGGGGA	5055	0.0	23.665152	5
GCAGAGT	17045	0.0	21.85417	9
ATGGGGC	5225	0.0	21.642212	5
CATGGGA	10650	0.0	20.565184	4
ATGGGGG	5790	0.0	20.558233	5
TACCGTA	415	2.910383E-11	20.073866	7
>>END_MODULE