FastQCFastQC Report
Mon 15 Aug 2016
SRR2921093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654355
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107940.2953736021815067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93420.25564018821378875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63990.17510614048169923No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT39450.10795338712303539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99150.049.048951
GTATCAA156250.043.6124881
TCAACGC200800.033.454024
ATCAACG202850.033.2334753
CAACGCA205000.032.710475
TATCAAC209200.032.5105252
AACGCAG207400.032.347296
GTACATG201600.031.4339371
TACATGG202150.030.9610442
ACATGGG205200.030.238593
CATGGGG114700.028.685594
ACGCAGA240000.027.7356557
CGCAGAG247000.026.925548
GCAGAGT259450.024.9918449
GAGTACT145700.023.08944112-13
AGTACTT151350.022.99396112-13
ATGGGGC46850.022.519145
AGAGTAC247250.022.03818110-11
CAGAGTA251850.021.96633310-11
GTACTTT155900.021.57907314-15
Produced by FastQC (version 0.11.5)