##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3654355
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31029579775364	33.0	31.0	34.0	28.0	34.0
2	31.37857268929811	33.0	31.0	34.0	28.0	34.0
3	31.486700115341833	34.0	31.0	34.0	28.0	34.0
4	34.96964389064554	37.0	35.0	37.0	32.0	37.0
5	34.718654865222454	37.0	35.0	37.0	32.0	37.0
6	34.46317913831579	37.0	35.0	37.0	31.0	37.0
7	34.415765299211486	37.0	35.0	37.0	30.0	37.0
8	34.397866107698896	37.0	35.0	37.0	30.0	37.0
9	35.954855234371045	39.0	37.0	39.0	30.0	39.0
10-11	35.92822796362149	39.0	37.0	39.0	30.0	39.0
12-13	35.86574525463453	39.0	37.0	39.0	30.0	39.0
14-15	37.1240918027942	40.0	37.0	41.0	31.0	41.0
16-17	37.1213654666829	40.0	37.0	41.0	31.0	41.0
18-19	37.15442588363747	40.0	37.5	41.0	31.0	41.0
20-21	37.15845162826272	40.0	38.0	41.0	31.0	41.0
22-23	37.108942070488496	40.0	37.5	41.0	30.5	41.0
24-25	37.055483388997516	40.0	37.0	41.0	30.0	41.0
26-27	36.933906804347146	40.0	37.0	41.0	30.0	41.0
28-29	36.80481822373579	40.0	37.0	41.0	30.0	41.0
30-31	36.64852443180807	40.0	36.5	41.0	30.0	41.0
32-33	36.49321603949261	40.0	36.0	41.0	30.0	41.0
34-35	36.3247006653705	40.0	36.0	41.0	29.5	41.0
36-37	36.12309942520636	40.0	35.5	41.0	28.5	41.0
38-39	35.969216729080784	40.0	35.0	41.0	27.5	41.0
40-41	35.81059530341196	40.0	35.0	41.0	27.0	41.0
42-43	35.631158576547705	40.0	35.0	41.0	26.5	41.0
44-45	35.41360541053072	40.0	35.0	41.0	25.5	41.0
46-47	35.075288799254594	39.5	35.0	41.0	23.5	41.0
48-49	34.75949216756446	39.0	35.0	41.0	22.5	41.0
50-51	34.53484021120006	39.0	34.5	41.0	21.0	41.0
52-53	34.31832019604006	39.0	34.0	41.0	20.0	41.0
54-55	34.09736054652599	38.5	34.0	41.0	18.5	41.0
56-57	33.84017152137655	38.0	34.0	41.0	17.5	41.0
58-59	33.57344565593655	38.0	33.5	40.0	15.0	41.0
60-61	33.28795136214188	37.0	33.0	40.0	13.0	41.0
62-63	32.984895282478035	37.0	33.0	40.0	11.0	41.0
64-65	32.660202689667535	36.0	33.0	39.5	8.0	41.0
66-67	32.31337007488325	35.5	32.5	39.0	7.0	41.0
68-69	31.955983066779226	35.0	32.0	39.0	3.5	41.0
70-71	31.57519138124238	35.0	32.0	37.5	2.0	40.0
72-73	31.203324663312678	35.0	31.5	37.0	2.0	39.5
74-75	30.88950019907754	35.0	31.0	37.0	2.0	39.0
76-77	30.60487432118664	35.0	31.0	36.0	2.0	39.0
78-79	30.31813151705294	35.0	31.0	36.0	2.0	37.5
80-81	30.29192388260035	35.0	31.0	35.5	2.0	37.0
82-83	30.110025024936	35.0	31.0	35.0	2.0	37.0
84-85	29.927804222632993	35.0	31.0	35.0	2.0	36.0
86-87	29.76973898266589	35.0	31.0	35.0	2.0	36.0
88-89	29.606691468125018	35.0	31.0	35.0	2.0	36.0
90-91	29.459103863746133	35.0	31.0	35.0	2.0	35.5
92-93	29.35650518354128	35.0	31.0	35.0	2.0	35.0
94-95	29.25282218065842	35.0	31.0	35.0	2.0	35.0
96-97	29.191180522965066	35.0	31.0	35.0	2.0	35.0
98-99	29.10419663661576	35.0	30.0	35.0	2.0	35.0
100-101	29.004540199296457	35.0	30.0	35.0	2.0	35.0
102-103	27.658337928307457	32.5	27.5	34.5	2.0	35.0
104-105	28.678445170214715	34.0	29.5	35.0	2.0	35.0
106-107	28.843447749329226	34.0	30.0	35.0	2.0	35.0
108-109	28.834497195811572	35.0	30.0	35.0	2.0	35.0
110-111	28.731727623616205	34.5	30.0	35.0	2.0	35.0
112-113	28.603705852332354	34.0	30.0	35.0	2.0	35.0
114-115	28.49122170670337	34.0	29.5	35.0	2.0	35.0
116-117	28.359493672618015	34.0	29.0	35.0	2.0	35.0
118-119	28.18975510042128	34.0	29.0	35.0	2.0	35.0
120-121	28.30590774568973	35.0	30.0	35.0	2.0	35.0
122-123	28.19160918958339	35.0	30.0	35.0	2.0	35.0
124-125	27.4649048874562	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66607.0
3	25998.0
4	11381.0
5	10143.0
6	12438.0
7	14987.0
8	15930.0
9	16987.0
10	20153.0
11	23692.0
12	25629.0
13	30149.0
14	40002.0
15	36562.0
16	19779.0
17	17126.0
18	15824.0
19	15200.0
20	15478.0
21	15810.0
22	16252.0
23	17428.0
24	22180.0
25	28190.0
26	34417.0
27	40704.0
28	49031.0
29	59123.0
30	71235.0
31	87759.0
32	111025.0
33	143775.0
34	199697.0
35	309238.0
36	608476.0
37	1033832.0
38	371376.0
39	742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.80009430871574	18.035609764790227	12.076806772681726	25.08748915381231
2	16.792098132389835	20.702636975457324	36.82619301293049	25.67907187922235
3	18.916717177176274	24.86460675002839	29.718404479039396	26.50027159375594
4	13.218424775806536	16.131968332416584	35.18380169722047	35.46580519455642
5	13.693240927811656	37.71846645129252	33.79733416939722	14.790958451498613
6	32.93091445657157	35.671677024961234	16.591035688875426	14.80637282959177
7	28.39236356272201	32.45338062319721	20.9395029090929	18.214752904987876
8	27.455072711269917	33.42140351799062	19.894447144752572	19.22907662598689
9	27.456001637708454	14.149346750281754	19.00642220364839	39.3882294083614
10-11	25.80042841671222	25.300161939054266	27.187220566191478	21.712189078042034
12-13	26.51661153025971	23.07473683403905	27.45412658238934	22.954525053311905
14-15	23.850414477646233	24.134012728464217	25.301438599165582	26.714134194723965
16-17	23.184903388253325	26.94468772237123	26.10047074278833	23.769938146587112
18-19	23.368018743998295	25.392022414848498	28.442328690806274	22.797630150346933
20-21	24.296975118092305	24.690296805965133	28.148188393698316	22.86453968224425
22-23	23.912267253624588	24.590140152490267	28.074320084490843	23.423272509394298
24-25	23.49014010102821	24.87913429416426	28.298436950431583	23.33228865437595
26-27	23.265086317500874	24.95447195750397	28.318339791721264	23.462101933273892
28-29	23.299275367277257	24.867082241366333	28.514372908726383	23.319269482630027
30-31	23.42715430048539	24.836090555665574	28.30225165668049	23.434503487168552
32-33	23.11609574511763	25.065636590072398	28.16896358236735	23.649304082442622
34-35	23.402401073712934	24.92833907738367	28.33386319192564	23.335396656977757
36-37	23.652842203166266	24.812868709611816	28.067202762538763	23.467086324683155
38-39	23.502946647237664	24.879038570807666	28.0510498559794	23.566964925975274
40-41	23.46330363748788	24.864652473207634	28.095091850736292	23.576952038568194
42-43	23.588500035719104	24.844621893430066	27.875777985371315	23.691100085479512
44-45	23.437004071379366	24.910020285041725	27.71048108848595	23.94249455509296
46-47	23.654369223298765	25.06773899431236	27.453158885749314	23.82473289663956
48-49	23.693031471796687	25.275612231380713	26.89842635542647	24.132929941396135
50-51	23.53911309861273	25.471508211854754	26.84650244736111	24.142876242171408
52-53	23.614651193787495	25.360057540236973	26.779268436536192	24.24602282943934
54-55	23.360908512542807	25.43054543075891	26.69922901621736	24.509317040480923
56-57	23.431345676709658	25.44739276839495	26.486089788230554	24.635171766664836
58-59	23.068219947244803	25.158845800794666	26.911773801828094	24.861160450132434
60-61	23.170970187070296	25.45392267936345	26.796371437599376	24.57873569596688
62-63	22.959152325116285	25.35724045594642	26.902888809245916	24.78071840969137
64-65	22.871231205897782	25.297440844742535	26.93829415470386	24.893033794655818
66-67	22.931485950436294	25.408521620176916	26.796741078185605	24.863251351201185
68-69	23.181902281934743	25.066399281376945	26.81237741723765	24.939321019450663
70-71	22.896657208427644	24.976073844061816	26.93059409811907	25.19667484939147
72-73	22.975003031586255	25.00590577638308	26.717554431002057	25.301536761028608
74-75	22.75075046144347	25.089387566534345	26.74547971509834	25.41438225692384
76-77	22.582489337852156	24.97579396877107	26.891751285150058	25.54996540822671
78-79	22.6798716305201	24.946302891104892	26.710069548977128	25.66375592939788
80-81	22.536513618444882	25.01464595382347	26.65488330517269	25.793957122558957
82-83	22.432716588398396	24.930363203362436	26.64131757266688	25.99560263557229
84-85	22.1990790187969	24.83228171717795	26.564646511781298	26.40399275224385
86-87	22.040666141579717	24.81115205864918	26.68039227169801	26.467789528073087
88-89	22.181199957356796	24.715199907816228	26.553852853297112	26.549747281529868
90-91	22.09877869458743	24.71970325747587	26.500062608238693	26.681455439698006
92-93	21.979540810203403	24.70959037693073	26.47849346488543	26.83237534798043
94-95	21.79720874631683	24.44096449608841	26.671509827927288	27.090316929667473
96-97	21.837808514330415	24.311000400157873	26.64717516459298	27.204015920918735
98-99	21.715720575352858	24.44178064710021	26.46439476290569	27.378104014641245
100-101	21.688167732538684	24.402969559998123	26.538700892091562	27.37016181537163
102-103	21.638449207067353	24.329611239797362	26.452797509517357	27.57914204361793
104-105	21.38731068445957	24.52836436928848	26.371829084006787	27.712495862245163
106-107	21.211208050926725	24.488500034828267	26.398649128547508	27.9016427856975
108-109	21.16147772788234	24.568042278346496	26.37989126634573	27.890588727425435
110-111	21.160589806498567	24.722346952614874	26.264853998380428	27.852209242506127
112-113	21.02984710243458	24.881311704776106	26.182996468226076	27.905844724563234
114-115	20.816231523271462	24.986952129710748	26.14497627572866	28.05184007128913
116-117	20.666833245437033	24.88246207436547	26.30476744609293	28.14593723410457
118-119	20.652001383661844	24.919664965550762	26.22578318106485	28.202550469722542
120-121	20.59020401394446	25.09317332797696	26.224470891253954	28.09215176682463
122-123	20.595342852291225	25.12429373042378	26.067236220551095	28.2131271967339
124-125	20.408611993057537	25.375209768705414	25.99320393862337	28.22297429961368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	171.0
2	363.5
3	680.0
4	529.0
5	349.5
6	639.5
7	1334.0
8	2115.0
9	2402.0
10	1984.0
11	1591.5
12	1669.0
13	1839.5
14	2104.5
15	2370.5
16	2625.5
17	3020.5
18	3417.5
19	3707.5
20	4178.5
21	4825.5
22	5491.0
23	6510.0
24	7832.0
25	9459.5
26	12031.5
27	15308.0
28	18200.0
29	21606.0
30	25726.0
31	30591.0
32	36794.0
33	41764.5
34	46067.0
35	51956.5
36	60301.5
37	67566.5
38	73141.5
39	79216.0
40	85648.0
41	90792.5
42	95595.0
43	105027.0
44	113252.0
45	118469.5
46	126905.5
47	135709.0
48	142637.5
49	149470.0
50	157213.0
51	160146.0
52	157809.5
53	156995.5
54	153884.5
55	147095.5
56	137932.0
57	126324.5
58	115978.0
59	105221.5
60	91390.0
61	76361.0
62	62017.5
63	47749.5
64	35063.5
65	26516.0
66	19675.5
67	14193.5
68	10635.0
69	8217.5
70	6526.0
71	5075.0
72	4048.0
73	3593.5
74	2966.0
75	2320.5
76	1672.0
77	977.0
78	594.5
79	362.0
80	200.0
81	121.0
82	95.5
83	69.0
84	37.5
85	30.5
86	26.0
87	14.0
88	10.0
89	13.0
90	12.0
91	8.5
92	11.0
93	10.5
94	5.5
95	12.0
96	18.5
97	12.0
98	4.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1849847647532875
2	0.011876240814042423
3	0.0
4	0.0039678684747376765
5	0.15080636665020228
6	0.0780712328167351
7	0.008072560000328376
8	0.012341439186942704
9	0.012834002170013587
10-11	0.005541333559547445
12-13	0.010590104136024004
14-15	0.01145208935639805
16-17	0.015187358644685588
18-19	0.01930573247536159
20-21	0.023451470916208195
22-23	0.02576378047562429
24-25	0.02517544135695629
26-27	0.022917861017881406
28-29	0.021344395933071636
30-31	0.024340820746752846
32-33	0.02476497220439722
34-35	0.02365670549248773
36-37	0.024737607594226614
38-39	0.019456237831299916
40-41	0.026872047187533776
42-43	0.023205189424672753
44-45	0.03203027620469276
46-47	0.027200422509581032
48-49	0.018361653424475727
50-51	0.014270644203970331
52-53	0.01543364013622103
54-55	0.017691220475295914
56-57	0.014954759458235448
58-59	0.013600211254790516
60-61	0.0170071052210308
62-63	0.015009488678576656
64-65	0.026858364882448478
66-67	0.020632916068635914
68-69	0.019182591729593867
70-71	0.02535331132306522
72-73	0.03152403091653657
74-75	0.01650085993287461
76-77	0.008400935322375631
78-79	0.014421149559908659
80-81	0.01684291756000717
82-83	0.015748333153182983
84-85	0.014626384136188194
86-87	0.009769165830905865
88-89	0.02144017206866875
90-91	0.01867634644143768
92-93	0.016240896136253867
94-95	0.011096349424180191
96-97	0.021809594305971916
98-99	0.022999954848393216
100-101	0.029731648950361966
102-103	0.020961291390683173
104-105	0.013723352000558239
106-107	0.019524649356726424
108-109	0.010412234169915073
110-111	0.025161759051870985
112-113	0.01825219498379331
114-115	0.01373703430564354
116-117	0.011712053153018796
118-119	0.007744184678281119
120-121	0.003940503864567071
122-123	0.0057055212205710725
124-125	8.893498305446515E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3654355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.29755425363797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.76499190496541	23.508108074069064
2	13.642014944972896	9.903435551881874
3	6.595563894644088	7.1820851489753865
4	3.9127729489263934	5.6809635358329125
5	2.5055067975093785	4.54718844577277
6	1.7291685496278952	3.7658753546281813
7	1.2034977624408858	3.057881772843097
8	0.9022197588277483	2.619869651580349
9	0.7013515924493305	2.291161273002447
>10	3.7376655960074388	24.211604511738685
>50	0.21059679519742144	5.2179061988973405
>100	0.0891369028478728	5.526802594036855
>500	0.004077777889033059	0.978779484243763
>1k	0.001208230479267383	0.7810171807730077
>5k	1.5102880990842287E-4	0.43145895425520864
>10k+	7.551440495421144E-5	0.29586226746907585
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10794	0.2953736021815067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9342	0.25564018821378875	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6399	0.17510614048169923	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	3945	0.10795338712303539	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.7364610170604663E-5
7	0.0	0.0	0.0	0.0	2.7364610170604663E-5
8	0.0	0.0	0.0	0.0	2.7364610170604663E-5
9	0.0	0.0	0.0	0.0	2.7364610170604663E-5
10-11	0.0	0.0	0.0	0.0	2.7364610170604663E-5
12-13	0.0	0.0	0.0	0.0	2.7364610170604663E-5
14-15	0.0	0.0	0.0	2.7364610170604663E-5	2.7364610170604663E-5
16-17	0.0	0.0	0.0	2.7364610170604663E-5	2.7364610170604663E-5
18-19	0.0	0.0	0.0	4.104691525590699E-5	2.7364610170604663E-5
20-21	0.0	0.0	0.0	6.841152542651166E-5	2.7364610170604663E-5
22-23	0.0	0.0	0.0	4.1046915255907E-4	2.7364610170604663E-5
24-25	0.0	0.0	0.0	0.0010398551864829772	2.7364610170604663E-5
26-27	0.0	0.0	0.0	0.0027501433221457684	2.7364610170604663E-5
28-29	0.0	0.0	0.0	0.008359888407119724	2.7364610170604663E-5
30-31	0.0	0.0	0.0	0.027501433221457684	2.7364610170604663E-5
32-33	0.0	0.0	0.0	0.06982080285029779	2.7364610170604663E-5
34-35	0.0	0.0	0.0	0.1385333389886861	2.7364610170604663E-5
36-37	0.0	0.0	0.0	0.2528216333662165	2.7364610170604663E-5
38-39	0.0	0.0	0.0	0.4379842680856129	2.7364610170604663E-5
40-41	0.0	0.0	0.0	0.652905916365542	2.7364610170604663E-5
42-43	0.0	0.0	0.0	0.8517645384753261	2.7364610170604663E-5
44-45	0.0	0.0	0.0	1.0926004725868177	2.7364610170604663E-5
46-47	0.0	0.0	0.0	1.3848545092088753	4.104691525590699E-5
48-49	0.0	0.0	0.0	1.7069907001372333	5.4729220341209326E-5
50-51	0.0	0.0	0.0	2.0569566996090964	1.0945844068241865E-4
52-53	0.0	0.0	0.0	2.3952516928431966	1.0945844068241865E-4
54-55	0.0	0.0	0.0	2.7618964222140434	1.0945844068241865E-4
56-57	0.0	0.0	0.0	3.1985535067063817	1.0945844068241865E-4
58-59	0.0	0.0	0.0	3.683837503471885	1.0945844068241865E-4
60-61	0.0	0.0	0.0	4.218637762341097	1.0945844068241865E-4
62-63	0.0	0.0	0.0	4.745420190430322	1.6418766102362797E-4
64-65	0.0	0.0	0.0	5.278469114248615	1.9155227119423262E-4
66-67	0.0	0.0	0.0	5.900589844172227	1.9155227119423262E-4
68-69	0.0	0.0	0.0	6.60443498237035	1.9155227119423262E-4
70-71	0.0	0.0	0.0	7.382903412503712	1.9155227119423262E-4
72-73	0.0	0.0	0.0	8.156993505009776	1.9155227119423262E-4
74-75	0.0	0.0	0.0	8.914240679955835	1.9155227119423262E-4
76-77	0.0	0.0	0.0	9.742622159040378	1.9155227119423262E-4
78-79	0.0	0.0	0.0	10.67550908436646	2.0523457627953496E-4
80-81	0.0	0.0	0.0	11.685016918170238	2.3259918645013962E-4
82-83	0.0	0.0	0.0	12.727116002687204	2.4628149153544196E-4
84-85	0.0	0.0	0.0	13.71822113615125	2.4628149153544196E-4
86-87	0.0	0.0	0.0	14.741452321955585	2.599637966207443E-4
88-89	0.0	0.0	0.0	15.850649430610874	2.87328406791349E-4
90-91	0.0	0.0	0.0	17.041584629845758	3.0101071187665126E-4
92-93	0.0	0.0	0.0	18.26907894826857	3.0101071187665126E-4
94-95	0.0	0.0	0.0	19.453884474825244	3.0101071187665126E-4
96-97	0.0	0.0	0.0	20.634489533720725	3.694222373031629E-4
98-99	0.0	0.0	0.0	21.85991782407566	3.8310454238846525E-4
100-101	0.0	0.0	0.0	23.182695715112516	3.8310454238846525E-4
102-103	0.0	0.0	0.0	24.53657348560827	3.8310454238846525E-4
104-105	0.0	0.0	0.0	25.839115247423962	3.8310454238846525E-4
106-107	0.0	0.0	0.0	27.09166186645797	3.8310454238846525E-4
108-109	0.0	0.0	0.0	28.359163244950203	3.8310454238846525E-4
110-111	0.0	0.0	0.0	29.688987523105993	3.8310454238846525E-4
112-113	0.0	0.0	0.0	31.05999553956854	3.8310454238846525E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9915	0.0	49.04895	1
GTATCAA	15625	0.0	43.612488	1
TCAACGC	20080	0.0	33.45402	4
ATCAACG	20285	0.0	33.233475	3
CAACGCA	20500	0.0	32.71047	5
TATCAAC	20920	0.0	32.510525	2
AACGCAG	20740	0.0	32.34729	6
GTACATG	20160	0.0	31.433937	1
TACATGG	20215	0.0	30.961044	2
ACATGGG	20520	0.0	30.23859	3
CATGGGG	11470	0.0	28.68559	4
ACGCAGA	24000	0.0	27.735655	7
CGCAGAG	24700	0.0	26.92554	8
GCAGAGT	25945	0.0	24.991844	9
GAGTACT	14570	0.0	23.089441	12-13
AGTACTT	15135	0.0	22.993961	12-13
ATGGGGC	4685	0.0	22.51914	5
AGAGTAC	24725	0.0	22.038181	10-11
CAGAGTA	25185	0.0	21.966333	10-11
GTACTTT	15590	0.0	21.579073	14-15
>>END_MODULE