FastQCFastQC Report
Mon 15 Aug 2016
SRR2921092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700907
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28310.10481664122459604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74750.033.0301441
GTACATG122300.031.5012191
TACATGG121000.030.7927282
CATGGGG82000.030.7759284
ACATGGG127150.028.6011853
AACGCAG84550.027.2400726
TATCAAC89700.026.8733482
TCAACGC86100.026.821794
CAACGCA88600.026.1321455
ATCAACG89150.026.0376913
ATGGGGA41250.025.539275
GGTATCA56050.024.685031
ACGCAGA94950.024.1928567
CGCAGAG99900.022.874978
CTATACT6000.021.8238144
GTATAGG5250.021.5831871
CTATGCG2408.864883E-619.8365219
GCAGAGT111400.019.6050369
GTATAGC7900.018.8727071
ATGGGGG38600.018.6576635
Produced by FastQC (version 0.11.5)