##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921092_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2700907
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.07701190748145	31.0	31.0	34.0	26.0	34.0
2	30.165374446435955	31.0	31.0	34.0	26.0	34.0
3	30.259291415809578	31.0	31.0	34.0	26.0	34.0
4	33.61582794224311	37.0	35.0	37.0	28.0	37.0
5	33.700611313162575	37.0	35.0	37.0	28.0	37.0
6	33.69617576614078	37.0	35.0	37.0	28.0	37.0
7	33.580779345605016	37.0	35.0	37.0	28.0	37.0
8	33.57230034207028	37.0	35.0	37.0	28.0	37.0
9	35.02844229734678	39.0	35.0	39.0	27.0	39.0
10-11	34.993740621206136	39.0	35.0	39.0	27.0	39.0
12-13	34.94520507370302	39.0	35.0	39.0	27.0	39.0
14-15	36.19360440770453	40.0	36.5	41.0	27.0	41.0
16-17	36.19121521029787	40.0	36.0	41.0	27.0	41.0
18-19	36.21590376862291	40.0	36.5	41.0	27.0	41.0
20-21	36.25168267548642	40.0	37.0	41.0	27.0	41.0
22-23	36.198283021222124	40.0	36.0	41.0	27.0	41.0
24-25	36.1884085605317	40.0	36.0	41.0	27.0	41.0
26-27	36.13106245420519	40.0	36.0	41.0	27.0	41.0
28-29	36.00862099287387	40.0	36.0	41.0	27.0	41.0
30-31	35.9101586985409	40.0	36.0	41.0	27.0	41.0
32-33	35.78909159034354	40.0	36.0	41.0	27.0	41.0
34-35	35.67247965220572	40.0	35.5	41.0	26.0	41.0
36-37	35.559985404902875	40.0	35.0	41.0	25.5	41.0
38-39	35.42783257624198	40.0	35.0	41.0	25.0	41.0
40-41	35.29014697655269	40.0	35.0	41.0	25.0	41.0
42-43	35.117060306037935	40.0	35.0	41.0	24.0	41.0
44-45	34.9444294083432	39.5	35.0	41.0	24.0	41.0
46-47	34.76343798583217	39.0	34.5	41.0	23.0	41.0
48-49	34.53462596083464	39.0	34.0	41.0	22.5	41.0
50-51	34.35556092823633	39.0	34.0	41.0	22.0	41.0
52-53	34.16812019073593	39.0	34.0	41.0	20.5	41.0
54-55	33.935096247297665	38.0	33.0	40.0	19.5	41.0
56-57	33.67893859359097	38.0	33.0	40.0	18.0	41.0
58-59	33.39135334907866	37.5	33.0	40.0	16.5	41.0
60-61	33.077986580063666	37.0	32.5	40.0	13.5	41.0
62-63	32.776012280319165	36.5	32.0	40.0	12.0	41.0
64-65	32.43858507530989	36.0	32.0	39.0	8.0	41.0
66-67	32.0775491344204	35.5	31.5	39.0	7.5	41.0
68-69	31.707142637639876	35.0	31.0	38.5	7.0	40.0
70-71	31.339829546148756	35.0	31.0	37.5	4.0	40.0
72-73	30.97721691268896	35.0	31.0	37.0	2.0	39.5
74-75	30.91354515353546	35.0	31.0	37.0	2.0	39.0
76-77	30.952501696652273	35.0	31.5	36.0	2.0	39.0
78-79	30.776333838965947	35.0	32.0	36.0	2.0	38.5
80-81	30.56308343826722	35.0	32.0	36.0	2.0	37.0
82-83	30.326693218241132	35.0	31.5	35.0	2.0	37.0
84-85	30.083403464095582	35.0	31.0	35.0	2.0	36.5
86-87	29.941138847061374	35.0	31.0	35.0	2.0	36.0
88-89	29.770067425498176	35.0	31.0	35.0	2.0	36.0
90-91	29.627973862113727	35.0	31.0	35.0	2.0	35.5
92-93	29.516457989853038	35.0	31.0	35.0	2.0	35.0
94-95	29.400109666863763	35.0	31.0	35.0	2.0	35.0
96-97	29.296349892832296	35.0	31.0	35.0	2.0	35.0
98-99	29.191811676596046	35.0	30.5	35.0	2.0	35.0
100-101	29.056543413009038	35.0	30.0	35.0	2.0	35.0
102-103	27.806454276285706	33.0	28.0	34.5	2.0	35.0
104-105	28.718380899453408	34.0	29.5	35.0	2.0	35.0
106-107	28.83612079201542	35.0	30.0	35.0	2.0	35.0
108-109	28.76261400336998	35.0	30.0	35.0	2.0	35.0
110-111	28.65277867768124	35.0	30.0	35.0	2.0	35.0
112-113	28.528141657598724	35.0	29.5	35.0	2.0	35.0
114-115	28.37286400457328	35.0	29.0	35.0	2.0	35.0
116-117	28.21212003967556	35.0	29.0	35.0	2.0	35.0
118-119	27.991591528327337	34.0	29.0	35.0	2.0	35.0
120-121	27.75857276833301	34.0	29.0	35.0	2.0	35.0
122-123	27.43491852922	34.0	28.5	35.0	2.0	35.0
124-125	26.17010822660684	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91521.0
3	13710.0
4	9186.0
5	9525.0
6	13765.0
7	13870.0
8	11666.0
9	12721.0
10	13928.0
11	15129.0
12	15837.0
13	15617.0
14	15591.0
15	13567.0
16	10405.0
17	9493.0
18	9149.0
19	9252.0
20	9475.0
21	10396.0
22	12400.0
23	16347.0
24	22795.0
25	28376.0
26	31215.0
27	34011.0
28	40152.0
29	47002.0
30	56058.0
31	68278.0
32	86286.0
33	111451.0
34	153385.0
35	235451.0
36	452498.0
37	737443.0
38	243576.0
39	380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24176206062838	17.77398165584512	11.74385310418981	25.24040317933669
2	16.544358879834313	20.544558949117565	37.61547054257678	25.29561162847134
3	19.522220291335053	24.408546411260936	28.561069535281568	27.50816376212244
4	13.244791641577342	16.290898068730357	35.177424907112375	35.286885382579925
5	14.15278299673818	37.98263031157379	33.373837407087066	14.490749284600964
6	33.503330438267064	35.6100935132352	16.666981750703286	14.21959429779445
7	28.744103434843026	32.6024502134754	20.392636090333134	18.260810261348446
8	26.67619735265861	34.882533121419506	19.472362740330443	18.96890678559144
9	26.607047474802513	16.05329401224484	18.553415407950734	38.78624310500191
10-11	25.463807952137014	26.25667579193209	26.9405518667017	21.338964389229197
12-13	25.87904821300001	23.899468952568135	26.76483132731472	23.456651507117133
14-15	23.409999866587412	25.150515343189067	24.10428269238466	27.335202097838863
16-17	23.506347505423232	27.72588071162919	24.974841045997294	23.79293073695028
18-19	23.65360350261529	26.840253394872445	26.4413361965941	23.064806905918168
20-21	24.762291033075627	26.03040569960494	25.85425704622406	23.353046221095372
22-23	24.349468548630913	25.883838009591614	25.71753715417129	24.049156287606184
24-25	23.88459170539937	25.99326757774903	26.08399538820368	24.03814532864792
26-27	23.719612920559776	26.06186044572503	26.144538687098436	24.07398794661676
28-29	23.834725762405174	26.281659457014733	26.056578243051025	23.827036537529068
30-31	23.951377681155822	26.33460053925575	25.765423194222297	23.948598585366135
32-33	23.73362322428206	26.431691696751436	25.738469645571627	24.096215433394875
34-35	23.909273103181732	26.33463403553713	25.890878489982384	23.865214371298755
36-37	24.038994210824125	26.186970037318563	25.816730321785986	23.957305430071326
38-39	23.915469166283334	26.30240498006518	25.836125962213412	23.945999891438078
40-41	23.993977557768496	26.28441031611893	25.935133124062236	23.786479002050342
42-43	23.82022226281524	26.332619085641916	25.819405626858877	24.02775302468397
44-45	23.731575130119204	26.285070124038175	25.90307219334801	24.080282552494612
46-47	23.765257894186	26.278282502243705	25.840322241538523	24.116137362031775
48-49	23.636799125061778	26.362404331418414	25.752603956339044	24.24819258718076
50-51	23.521603029763007	26.516557994197303	25.842645425919635	24.11919355012006
52-53	23.81441396643636	26.296783067222517	25.653389101060352	24.235413865280773
54-55	23.733662484527034	26.275110392811836	25.616227470055957	24.374999652605172
56-57	23.612548662104796	26.471184437020444	25.64309947729349	24.27316742358127
58-59	23.68206517963903	26.20561585561822	25.71406872138654	24.398250243356213
60-61	23.654168875984375	26.18879819415836	25.839568518862293	24.317464410994972
62-63	23.574621700019122	26.141458772750248	25.894687421720175	24.389232105510455
64-65	23.60166415114823	26.212214656008797	25.754863948029456	24.431257244813516
66-67	23.53661037464842	26.30710318313792	25.714413272371157	24.441873169842506
68-69	23.732480866744353	26.121919272575017	25.771564397230655	24.37403546344997
70-71	23.538311932880593	26.068183919212757	25.87210341727335	24.5214007306333
72-73	23.649197081211362	26.05037751834801	25.813078683336922	24.487346717103705
74-75	23.48971726532637	26.238394323332376	25.84197029191342	24.429918119427835
76-77	23.449252891751016	26.003969556848933	26.009250193993932	24.537527357406123
78-79	23.486735962484	26.15490095714778	25.982337588043908	24.37602549232431
80-81	23.541780572810413	26.100979498654247	25.979456967146675	24.377782961388668
82-83	23.52481781984129	25.93291249238021	26.11817762403351	24.42409206374499
84-85	23.620189532801366	25.908553990166677	25.924118322276176	24.547138154755785
86-87	23.43960396773345	25.83873394175865	26.140816001363582	24.580846089144316
88-89	23.43584330944303	25.82379314202814	26.094957844345274	24.645405704183556
90-91	23.49070744238355	25.859675061558214	26.003523964626957	24.646093531431283
92-93	23.55820472857502	25.861994871986756	25.983749065958435	24.59605133347979
94-95	23.39254434804456	25.905813280011795	26.048572647194078	24.653069724749564
96-97	23.47324687527905	25.77581227182673	26.069860064226617	24.6810807886676
98-99	23.392223883508493	26.00375725279279	25.89466317086423	24.70935569283449
100-101	23.320271101525964	26.141101748300983	25.93605250090787	24.60257464926518
102-103	23.284511395963374	26.257857033570886	25.858869580003653	24.598761990462087
104-105	23.21953637618072	26.435364176752714	25.744313162349847	24.600786284716722
106-107	23.154425024127434	26.51918531629125	25.798317443842695	24.52807221573863
108-109	23.102046306814657	26.618960702704737	25.748917610956575	24.530075379524035
110-111	23.19749924871485	26.8473370044714	25.601704370497153	24.353459376316604
112-113	23.284126019038982	27.078472717006257	25.414460748080113	24.222940515874647
114-115	23.378472795428358	27.220505614594632	25.307817484866895	24.093204105110118
116-117	23.2712484361131	27.188001205719054	25.46753571813001	24.073214640037836
118-119	23.4744903790916	27.272075514131856	25.19707824200261	24.056355864773934
120-121	23.506174843611337	27.394279557721717	25.24001956143906	23.859526037227887
122-123	23.599848520106658	27.48187280728408	25.066680055789657	23.851598616819604
124-125	23.790153484084076	27.670220010840712	24.805566751446587	23.734059753628628
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2433.0
1	1781.0
2	985.0
3	795.5
4	639.5
5	492.5
6	466.0
7	497.5
8	584.5
9	644.5
10	614.0
11	594.5
12	647.0
13	744.0
14	823.0
15	885.0
16	1051.5
17	1248.0
18	1486.0
19	1811.5
20	2162.5
21	2679.5
22	3488.0
23	4369.0
24	5183.0
25	6231.5
26	7837.0
27	10028.0
28	12095.5
29	14410.0
30	16983.5
31	20199.0
32	24212.5
33	27582.5
34	31536.0
35	36293.5
36	41553.0
37	46831.0
38	52381.5
39	57623.5
40	62761.0
41	68170.5
42	73131.0
43	80050.0
44	88080.5
45	95239.5
46	102181.0
47	107811.5
48	112555.0
49	116945.0
50	120701.0
51	122395.0
52	120606.5
53	119590.5
54	116766.5
55	110427.5
56	102820.5
57	94072.0
58	86486.5
59	77778.5
60	65753.5
61	53065.0
62	41986.0
63	31551.5
64	22485.5
65	16735.5
66	12590.5
67	9305.5
68	6934.0
69	5041.5
70	3697.5
71	2709.0
72	2041.0
73	1691.5
74	1394.0
75	1017.0
76	722.0
77	501.5
78	341.0
79	259.5
80	173.0
81	103.0
82	90.5
83	77.0
84	61.5
85	46.5
86	38.5
87	29.0
88	23.0
89	20.5
90	18.0
91	20.5
92	16.0
93	10.0
94	12.0
95	12.0
96	8.5
97	8.0
98	9.5
99	8.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27823986534893647
2	0.06819931230508862
3	0.07738141298460109
4	0.08204651252338567
5	0.08934035862767581
6	0.11899706283852055
7	0.1087782733726115
8	0.10940769156435226
9	0.11544270128516086
10-11	0.09396843356694622
12-13	0.08434203769326377
14-15	0.0929687693800638
16-17	0.09491256085455738
18-19	0.08708185805731185
20-21	0.08641541526605692
22-23	0.06971732088516931
24-25	0.08054701624306206
26-27	0.09235786348808012
28-29	0.08610070617018653
30-31	0.08099131143723201
32-33	0.08963655542378912
34-35	0.082805516813426
36-37	0.08330534890686721
38-39	0.07354936693488522
40-41	0.08641541526605692
42-43	0.0751599370137513
44-45	0.07180921075771954
46-47	0.06255306087917874
48-49	0.06460792615221479
50-51	0.06731072191674871
52-53	0.07752951138265776
54-55	0.08324981200759597
56-57	0.09078431800872817
58-59	0.06199769188646629
60-61	0.07812190497488436
62-63	0.08349047190443802
64-65	0.07380853913148434
66-67	0.068736168998044
68-69	0.07671497019334617
70-71	0.07462308032079594
72-73	0.05805457203820791
74-75	0.08447162379156335
76-77	0.08771127624905263
78-79	0.07029120217763885
80-81	0.08258336921634102
82-83	0.08671161206217022
84-85	0.0899512645196595
86-87	0.07912156916176677
88-89	0.060164974210515205
90-91	0.08300915211075391
92-93	0.05983175281488774
94-95	0.08552682487771701
96-97	0.07501183861569465
98-99	0.06888426739610064
100-101	0.08467525908889126
102-103	0.05827671963529288
104-105	0.06507073364614184
106-107	0.08202800022362859
108-109	0.08747061635221057
110-111	0.08138006973213073
112-113	0.07101318186816502
114-115	0.08406435319690755
116-117	0.07784422047852814
118-119	0.07092062036937961
120-121	0.10150293956807843
122-123	0.08141709433164489
124-125	0.10161401336662092
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2700907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.795622578999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.1218998254345	31.878067135954108
2	14.639545389115499	13.70133281514348
3	6.326898023563386	8.882133960194608
4	3.373211894358656	6.314062027495917
5	1.9593815914298727	4.584524072039538
6	1.3160304215239338	3.6950677744868985
7	0.8760297432713374	2.8696050063872027
8	0.6422102311178562	2.4042102073410336
9	0.4770264992779711	2.0090476818353764
>10	2.131666277674412	17.081816477838725
>50	0.09929627012652388	3.1736812266438283
>100	0.035137284429748214	2.6753032069477474
>500	0.0012697513723734174	0.37808928986569706
>1k	3.967973038437951E-4	0.35305911782592847
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2831	0.10481664122459604	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.404919902832641E-5
2	0.0	0.0	0.0	0.0	7.404919902832641E-5
3	0.0	0.0	0.0	0.0	7.404919902832641E-5
4	0.0	0.0	0.0	0.0	7.404919902832641E-5
5	0.0	0.0	0.0	0.0	7.404919902832641E-5
6	0.0	0.0	0.0	0.0	7.404919902832641E-5
7	0.0	0.0	0.0	0.0	7.404919902832641E-5
8	0.0	0.0	0.0	0.0	7.404919902832641E-5
9	0.0	0.0	0.0	0.0	7.404919902832641E-5
10-11	0.0	0.0	0.0	0.0	7.404919902832641E-5
12-13	0.0	0.0	0.0	0.0	1.1107379854248962E-4
14-15	0.0	0.0	0.0	0.0	1.1107379854248962E-4
16-17	0.0	0.0	0.0	1.8512299757081602E-5	1.1107379854248962E-4
18-19	0.0	0.0	0.0	7.404919902832641E-5	1.1107379854248962E-4
20-21	0.0	0.0	0.0	1.1107379854248962E-4	1.4809839805665282E-4
22-23	0.0	0.0	0.0	3.3322139562746886E-4	1.4809839805665282E-4
24-25	0.0	0.0	0.0	6.664427912549377E-4	1.4809839805665282E-4
26-27	0.0	0.0	0.0	0.0015550331795948545	1.4809839805665282E-4
28-29	0.0	0.0	0.0	0.004665099538784564	1.4809839805665282E-4
30-31	0.0	0.0	0.0	0.014735790606636955	1.4809839805665282E-4
32-33	0.0	0.0	0.0	0.042171018846631894	1.4809839805665282E-4
34-35	0.0	0.0	0.0	0.08902564953180543	1.4809839805665282E-4
36-37	0.0	0.0	0.0	0.16879514918507005	1.4809839805665282E-4
38-39	0.0	0.0	0.0	0.30511972459621894	1.85122997570816E-4
40-41	0.0	0.0	0.0	0.4637701335144083	1.85122997570816E-4
42-43	0.0	0.0	0.0	0.6183663487857968	1.85122997570816E-4
44-45	0.0	0.0	0.0	0.8044890105434952	1.85122997570816E-4
46-47	0.0	0.0	0.0	1.034226650528878	2.0363529732789762E-4
48-49	0.0	0.0	0.0	1.281069655489804	2.2214759708497924E-4
50-51	0.0	0.0	0.0	1.5418339098680554	2.2214759708497924E-4
52-53	0.0	0.0	0.0	1.8015799877596672	3.70245995141632E-4
54-55	0.0	0.0	0.0	2.0971288533814754	3.70245995141632E-4
56-57	0.0	0.0	0.0	2.455952759572988	3.70245995141632E-4
58-59	0.0	0.0	0.0	2.8482098791257897	3.70245995141632E-4
60-61	0.0	0.0	0.0	3.2692536248008537	3.70245995141632E-4
62-63	0.0	0.0	0.0	3.6974431181821514	3.70245995141632E-4
64-65	0.0	0.0	0.0	4.151716441921177	3.8875829489871364E-4
66-67	0.0	0.0	0.0	4.6807794566788115	4.0727059465579525E-4
68-69	0.0	0.0	0.0	5.280503919609227	4.0727059465579525E-4
70-71	0.0	0.0	0.0	5.934395371628864	4.0727059465579525E-4
72-73	0.0	0.0	0.0	6.596487772440888	4.0727059465579525E-4
74-75	0.0	0.0	0.0	7.261523628914286	4.0727059465579525E-4
76-77	0.0	0.0	0.0	8.000034803123544	4.0727059465579525E-4
78-79	0.0	0.0	0.0	8.83210714030509	4.0727059465579525E-4
80-81	0.0	0.0	0.0	9.712237407656021	4.0727059465579525E-4
82-83	3.7024599514163204E-5	0.0	0.0	10.627559556845164	4.442951941699585E-4
84-85	3.7024599514163204E-5	0.0	0.0	11.542659558437222	4.442951941699585E-4
86-87	3.7024599514163204E-5	1.8512299757081602E-5	0.0	12.49111872419154	4.442951941699585E-4
88-89	3.7024599514163204E-5	3.7024599514163204E-5	0.0	13.519180778901308	4.442951941699585E-4
90-91	3.7024599514163204E-5	3.7024599514163204E-5	0.0	14.608240861310662	4.442951941699585E-4
92-93	3.7024599514163204E-5	3.7024599514163204E-5	0.0	15.71377689050382	4.442951941699585E-4
94-95	3.7024599514163204E-5	3.7024599514163204E-5	0.0	16.815147652251632	4.442951941699585E-4
96-97	3.7024599514163204E-5	3.7024599514163204E-5	0.0	17.935437984351182	5.183443931982849E-4
98-99	3.7024599514163204E-5	3.7024599514163204E-5	0.0	19.11974384901072	5.183443931982849E-4
100-101	3.7024599514163204E-5	3.7024599514163204E-5	0.0	20.372267538275104	5.183443931982849E-4
102-103	3.7024599514163204E-5	3.7024599514163204E-5	0.0	21.64395145778807	5.183443931982849E-4
104-105	3.7024599514163204E-5	3.7024599514163204E-5	0.0	22.89108806782314	5.183443931982849E-4
106-107	3.7024599514163204E-5	3.7024599514163204E-5	0.0	24.11104862181482	5.183443931982849E-4
108-109	3.7024599514163204E-5	3.7024599514163204E-5	0.0	25.35783349815451	5.183443931982849E-4
110-111	3.7024599514163204E-5	3.7024599514163204E-5	0.0	26.653268698255808	5.183443931982849E-4
112-113	3.7024599514163204E-5	3.7024599514163204E-5	0.0	27.978786385462364	5.183443931982849E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7475	0.0	33.030144	1
GTACATG	12230	0.0	31.501219	1
TACATGG	12100	0.0	30.792728	2
CATGGGG	8200	0.0	30.775928	4
ACATGGG	12715	0.0	28.601185	3
AACGCAG	8455	0.0	27.240072	6
TATCAAC	8970	0.0	26.873348	2
TCAACGC	8610	0.0	26.82179	4
CAACGCA	8860	0.0	26.132145	5
ATCAACG	8915	0.0	26.037691	3
ATGGGGA	4125	0.0	25.53927	5
GGTATCA	5605	0.0	24.68503	1
ACGCAGA	9495	0.0	24.192856	7
CGCAGAG	9990	0.0	22.87497	8
CTATACT	600	0.0	21.823814	4
GTATAGG	525	0.0	21.583187	1
CTATGCG	240	8.864883E-6	19.836521	9
GCAGAGT	11140	0.0	19.605036	9
GTATAGC	790	0.0	18.872707	1
ATGGGGG	3860	0.0	18.657663	5
>>END_MODULE