FastQCFastQC Report
Mon 15 Aug 2016
SRR2921092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700907
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73700.27287129841938285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59500.22029636710927106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.15691025274102366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61950.046.4034771
GTATCAA94400.042.3299371
TCAACGC118050.033.267314
ATCAACG119100.033.0740973
TATCAAC120900.032.680872
CAACGCA120000.032.5777365
AACGCAG124450.031.5214186
TACATGG110500.028.2062782
GTACATG112650.028.1130581
ACGCAGA140100.027.813337
CATGGGG79400.027.1697964
CGCAGAG147200.026.5131868
ACATGGG117700.025.7208633
GCAGAGT161050.023.8271949
AGTACTT92400.023.46839312-13
GAGTACT89500.023.46440112-13
AGAGTAC143900.022.46945810-11
GTACTTT97200.021.88223614-15
CAGAGTA151250.021.71188410-11
TACTTTT106400.020.10199714-15
Produced by FastQC (version 0.11.5)