FastQCFastQC Report
Mon 15 Aug 2016
SRR2921091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020946
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28820.14260648231075942No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23190.11474824166504201No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21460.10618789418420879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG53050.029.732954
GTACATG93300.029.537571
TACATGG91250.028.7010172
GTATCAA69950.028.0557981
CGCGTAA557.755836E-427.04473510-11
AACGCAG72150.026.065366
ACATGGG100450.025.4205443
TCAACGC75000.025.2373264
TATCAAC77200.025.0578352
ATCAACG78250.024.2651983
CAACGCA78900.024.2149755
ACGCAGA81300.023.2021317
TCGCGTA1300.002051535522.8868379
ATGGGGG26700.022.5146985
CGCAGAG85000.021.912138
GGTATCA52000.021.5659681
GTATCGC1400.003152434721.2546986
GAGTACT25150.020.1088112-13
GTCTAGA8350.020.0025771
GCAGAGT91150.019.6502919
Produced by FastQC (version 0.11.5)