##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921091_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2020946
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.698646574426036	31.0	30.0	34.0	25.0	34.0
2	29.800136173851257	31.0	30.0	34.0	25.0	34.0
3	29.896665225097554	31.0	31.0	34.0	25.0	34.0
4	33.21205861017563	37.0	35.0	37.0	26.0	37.0
5	33.34731556409721	37.0	35.0	37.0	27.0	37.0
6	33.30655841373297	37.0	35.0	37.0	26.0	37.0
7	33.1978063738467	37.0	35.0	37.0	26.0	37.0
8	33.17871976787109	37.0	35.0	37.0	26.0	37.0
9	34.57450916550962	39.0	35.0	39.0	25.0	39.0
10-11	34.566694013595615	39.0	35.0	39.0	26.0	39.0
12-13	34.510972089308666	39.0	35.0	39.0	25.5	39.0
14-15	35.72398025479157	40.0	36.0	41.0	25.5	41.0
16-17	35.72034161229443	40.0	36.0	41.0	26.0	41.0
18-19	35.750040575057426	40.0	36.0	41.0	26.0	41.0
20-21	35.7822900760337	40.0	36.0	41.0	26.0	41.0
22-23	35.731517071708	40.0	36.0	41.0	26.0	41.0
24-25	35.72618986355894	40.0	36.0	41.0	26.0	41.0
26-27	35.64835849151832	40.0	36.0	41.0	26.0	41.0
28-29	35.51389794680313	40.0	35.5	41.0	25.5	41.0
30-31	35.39570453639038	40.0	35.0	41.0	25.0	41.0
32-33	35.24943640255603	40.0	35.0	41.0	24.5	41.0
34-35	35.115975884560996	40.0	35.0	41.0	24.0	41.0
36-37	34.97609807486197	40.0	35.0	41.0	23.5	41.0
38-39	34.82789718280449	40.0	35.0	41.0	22.5	41.0
40-41	34.68020199451148	40.0	35.0	41.0	21.5	41.0
42-43	34.48618072922285	39.0	34.0	41.0	19.5	41.0
44-45	34.30095633431077	39.0	34.0	41.0	18.0	41.0
46-47	34.104100257997985	39.0	34.0	41.0	16.0	41.0
48-49	33.854003521123275	39.0	33.0	41.0	13.0	41.0
50-51	33.66406524469234	39.0	33.0	41.0	9.0	41.0
52-53	33.47616190635475	38.0	33.0	40.0	8.0	41.0
54-55	33.252580969506354	38.0	33.0	40.0	7.0	41.0
56-57	33.00070734200716	38.0	32.5	40.0	3.5	41.0
58-59	32.71509110090027	37.0	32.0	40.0	2.0	41.0
60-61	32.40205700696605	37.0	32.0	40.0	2.0	41.0
62-63	32.1047022038194	36.0	31.0	40.0	2.0	41.0
64-65	31.783623857342057	36.0	31.0	39.0	2.0	41.0
66-67	31.43105530776181	35.0	31.0	39.0	2.0	41.0
68-69	31.055297123228428	35.0	30.5	38.5	2.0	40.0
70-71	30.688389744208898	35.0	30.0	37.5	2.0	40.0
72-73	30.326519115305405	35.0	30.0	37.0	2.0	39.0
74-75	30.26358027379257	35.0	30.0	37.0	2.0	39.0
76-77	30.30012528786024	35.0	31.0	36.0	2.0	39.0
78-79	30.12404067204171	35.0	31.0	36.0	2.0	38.5
80-81	29.906585331819848	35.0	31.0	35.5	2.0	37.0
82-83	29.665744408806567	35.0	31.0	35.0	2.0	37.0
84-85	29.41827837062445	35.0	30.5	35.0	2.0	36.5
86-87	29.259828070616436	35.0	30.0	35.0	2.0	36.0
88-89	29.085521582466825	35.0	30.0	35.0	2.0	36.0
90-91	28.94159987451421	35.0	30.0	35.0	2.0	35.5
92-93	28.819266323790938	35.0	30.0	35.0	2.0	35.0
94-95	28.703920589664442	35.0	30.0	35.0	2.0	35.0
96-97	28.60322096681455	35.0	29.5	35.0	2.0	35.0
98-99	28.4924047945863	34.0	29.0	35.0	2.0	35.0
100-101	28.363247706767027	34.0	29.0	35.0	2.0	35.0
102-103	27.10058507253534	32.5	26.0	34.5	2.0	35.0
104-105	28.022104994393715	34.0	29.0	35.0	2.0	35.0
106-107	28.152784141684144	34.0	29.0	35.0	2.0	35.0
108-109	28.083581401977092	34.0	29.0	35.0	2.0	35.0
110-111	27.984420167584883	34.0	29.0	35.0	2.0	35.0
112-113	27.865539455284804	34.0	29.0	35.0	2.0	35.0
114-115	27.713795667969357	34.0	29.0	35.0	2.0	35.0
116-117	27.558217042909607	34.0	27.5	35.0	2.0	35.0
118-119	27.340257235967712	34.0	27.0	35.0	2.0	35.0
120-121	27.11942253776202	34.0	27.0	35.0	2.0	35.0
122-123	26.803742158375336	34.0	26.0	35.0	2.0	35.0
124-125	25.4911519159839	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80274.0
3	11426.0
4	8069.0
5	8950.0
6	14312.0
7	14327.0
8	10984.0
9	11540.0
10	11864.0
11	12393.0
12	12508.0
13	12501.0
14	13046.0
15	11848.0
16	9067.0
17	8141.0
18	7692.0
19	7388.0
20	7678.0
21	8412.0
22	9714.0
23	12633.0
24	16927.0
25	21600.0
26	23569.0
27	25529.0
28	30073.0
29	35416.0
30	41982.0
31	51434.0
32	64843.0
33	84096.0
34	116241.0
35	177277.0
36	331029.0
37	530817.0
38	165137.0
39	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.601366777637125	17.7098038904335	11.517523127392073	25.171306204537302
2	16.557228583071847	20.813751913165202	37.53225332857322	25.09676617518973
3	19.438402890437903	24.979349770321907	28.38812329524183	27.194124043998357
4	13.139787225402207	16.806390171283454	35.063534245299934	34.99028835801441
5	14.00278640239593	38.46077269424706	33.11465406957883	14.421786833778171
6	33.093808538371945	36.226622427903514	16.499516210778516	14.180052822946026
7	28.23585826786476	33.4291232817131	20.194654554299326	18.14036389612281
8	26.28824127652808	35.56755139416535	19.242934556188924	18.901272773117654
9	26.42971830448639	16.815529343922574	18.453109619043985	38.301642732547045
10-11	25.03330306195001	26.944932250960086	26.733585658149984	21.288179028939922
12-13	25.602763101169014	24.70333880502511	26.41457759719847	23.27932049660741
14-15	23.29343366777597	25.96468218373526	23.961320091670004	26.78056405681876
16-17	23.31944567927865	28.334132845486454	24.898492500642043	23.44792897459285
18-19	23.338371909311576	27.423332998198692	26.48729204111359	22.751003051376138
20-21	24.430931906964073	26.57972252660513	26.037850788403322	22.951494778027477
22-23	23.99340121116446	26.517910839102406	25.65238307340843	23.836304876324704
24-25	23.684736392610134	26.540659545285827	25.94577769831933	23.82882636378471
26-27	23.486090852131877	26.717203361078163	26.012124844291346	23.78458094249862
28-29	23.576593134523655	26.96176100741067	26.034623055523774	23.427022802541902
30-31	23.888751872013692	26.864906973795772	25.79724011695628	23.449101037234254
32-33	23.348836587316192	27.077888806974208	25.840913880542644	23.732360725166952
34-35	23.541420226039037	27.04522093172696	25.973961012091884	23.43939783014212
36-37	23.747700783392748	26.950954007743793	25.71358669866668	23.587758510196785
38-39	23.50550378097881	27.10761620681945	25.806576211712034	23.580303800489705
40-41	23.62431121460983	27.03094494966654	25.91353520865876	23.43120862706487
42-43	23.375209782539258	27.02645352248963	25.872965251741526	23.72537144322959
44-45	23.502503935533998	26.989146889133075	25.810120821760297	23.698228353572635
46-47	23.358799452270894	27.072781076954648	25.775650415933953	23.792769054840498
48-49	23.386568877260498	27.017228351665544	25.775236212713743	23.82096655836021
50-51	23.263129722477576	27.15797062733145	25.637592499840927	23.941307150350045
52-53	23.33711353990759	27.050077007264928	25.495471180464417	24.11733827236306
54-55	23.270764653925117	26.863756809955653	25.554843775574867	24.310634760544367
56-57	23.43321922684787	26.78008965238342	25.477997870749704	24.308693250019004
58-59	23.424291263433872	26.494331840474544	25.690614300250296	24.390762595841288
60-61	23.4599922991877	26.564212022428922	25.627337636048715	24.34845804233466
62-63	23.30213014214402	26.465065475690803	25.696265002034234	24.53653938013094
64-65	23.286437287061247	26.334284129625228	25.769625029712383	24.60965355360114
66-67	23.36273619696609	26.377604668937387	25.707975020234226	24.551684113862294
68-69	23.606952005391886	26.265703839538933	25.718121496673895	24.40922265839528
70-71	23.339727274910683	26.115335085208947	25.89318543825406	24.65175220162631
72-73	23.48330854005334	26.05143683432271	25.89299750136221	24.57225712426174
74-75	23.25290631303739	26.064005499373742	26.025573276530245	24.657514911058627
76-77	23.077441210848505	26.01610634546427	26.34618241971591	24.560270023971313
78-79	23.189568045541133	25.809044942457078	26.46469408239574	24.536692929606048
80-81	23.186945542374886	25.937857659680574	26.266824088514127	24.608372709430405
82-83	23.19457366087956	25.84417575130088	26.29575984918141	24.66549073863815
84-85	23.228115290044055	25.684443937049956	26.21095927523595	24.876481497670046
86-87	23.110166322894717	25.68456839493578	26.35337610467175	24.851889177497753
88-89	23.234885415317947	25.54405721547178	26.309283699982693	24.91177366922757
90-91	23.276165836339295	25.59910973915942	26.254275552469597	24.870448872031687
92-93	23.2499828558937	25.56093411489002	26.278758464670393	24.91032456454589
94-95	23.075684918362015	25.61849115521432	26.30755115761635	24.99827276880732
96-97	22.989279010417427	25.682141811017516	26.200623812585842	25.12795536597921
98-99	22.89673937936917	25.790086792251955	26.094769562919122	25.218404265459753
100-101	22.95285955829113	26.03332676293782	26.064428836178248	24.949384842592803
102-103	22.781341730028494	26.203610615227586	26.045689832094983	24.969357822648945
104-105	22.552519445434072	26.323808410885235	25.991483794873293	25.1321883488074
106-107	22.464362945634967	26.560268698945105	26.052471472156334	24.92289688326359
108-109	22.512501937757015	26.552199088003636	26.011632980649175	24.92366599359017
110-111	22.538932529000526	26.814224527610893	25.7422815275423	24.904561415846285
112-113	22.594150961428472	26.934283364274208	25.701616732930816	24.769948941366504
114-115	22.592928996778884	26.938761158016934	25.699518021212615	24.768791823991567
116-117	22.575781623929615	26.8593583288908	25.695170938721258	24.869689108458328
118-119	22.640854015129868	26.839994473740113	25.648060436287324	24.871091074842692
120-121	22.813578313217203	26.91658022823708	25.5933044643839	24.676536994161815
122-123	22.914514953636903	26.973895176093592	25.47010003097724	24.641489839292262
124-125	23.012333435953042	27.067826343676295	25.22931542086918	24.690524799501482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2294.0
1	1644.5
2	840.0
3	624.0
4	514.0
5	432.0
6	375.5
7	384.5
8	480.5
9	507.5
10	462.5
11	473.0
12	532.0
13	597.0
14	664.5
15	769.0
16	908.0
17	1060.0
18	1288.0
19	1550.5
20	1858.5
21	2298.0
22	2889.0
23	3614.0
24	4349.5
25	5310.5
26	6865.5
27	8786.0
28	10468.5
29	12393.0
30	14929.0
31	17424.0
32	20241.5
33	23676.5
34	27301.5
35	30610.0
36	34253.5
37	38276.0
38	41282.5
39	43959.0
40	47821.0
41	51705.5
42	55002.5
43	60195.0
44	65864.0
45	70505.5
46	75274.0
47	79643.5
48	83759.5
49	86985.5
50	90728.5
51	91554.0
52	88517.5
53	86082.0
54	82367.5
55	77240.5
56	70967.5
57	64350.0
58	58686.0
59	52267.5
60	44741.0
61	37101.5
62	30096.5
63	23519.5
64	17969.5
65	13798.0
66	10359.5
67	8032.5
68	6301.0
69	4951.0
70	3998.0
71	3160.0
72	2499.0
73	2136.5
74	1828.0
75	1430.5
76	1010.5
77	656.5
78	459.5
79	327.0
80	228.5
81	161.5
82	118.0
83	92.0
84	66.5
85	52.5
86	40.0
87	28.5
88	23.5
89	22.0
90	17.5
91	13.5
92	11.0
93	9.0
94	10.5
95	13.0
96	12.0
97	12.5
98	14.5
99	21.5
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2817987219846547
2	0.06863122517870343
3	0.07907188019867924
4	0.08510865703487376
5	0.09178869697656444
6	0.1239518522513714
7	0.11346171545404975
8	0.11296689768059116
9	0.11969641939962769
10-11	0.09834503247489047
12-13	0.08736007790411025
14-15	0.0972316924846087
16-17	0.09928518624446175
18-19	0.09188766053125616
20-21	0.09072483876362852
22-23	0.07345569846992447
24-25	0.08518287970089256
26-27	0.0965389476017667
28-29	0.09050217076557217
30-31	0.08560347480833233
32-33	0.09438649028722193
34-35	0.08815178634164396
36-37	0.08807756367562518
38-39	0.07726579532555546
40-41	0.08966098055069259
42-43	0.07882447131194995
44-45	0.07545971045243167
46-47	0.06603343186804596
48-49	0.06769107140913216
50-51	0.07004145583306036
52-53	0.08194182328473892
54-55	0.08666733302126826
56-57	0.09527716227944735
58-59	0.06586024564733546
60-61	0.0830551632750207
62-63	0.08716215079472683
64-65	0.07847809887052895
66-67	0.07281243536442834
68-69	0.0799130704135588
70-71	0.07850283975920187
72-73	0.06162955368426469
74-75	0.08941357166396331
76-77	0.09233299652736886
78-79	0.07407422068674768
80-81	0.08624673791382848
82-83	0.0906258752089368
84-85	0.09483182628333463
86-87	0.08317886771838534
88-89	0.06346037944606141
90-91	0.08721163257207268
92-93	0.06440053321563267
94-95	0.08998261210344066
96-97	0.07865128509123945
98-99	0.07266399003239077
100-101	0.08847341789439203
102-103	0.06247074389914427
104-105	0.06850752073533879
106-107	0.08582614280638869
108-109	0.09198662408594788
110-111	0.08488598903681742
112-113	0.07437111135082283
114-115	0.08758274590216661
116-117	0.08117485573587814
118-119	0.07407422068674768
120-121	0.10727649328581762
122-123	0.08518287970089256
124-125	0.107152788842453
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2020946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.03589379502613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.43734356697591	35.43796891692924
2	14.358005615813937	14.655472994341375
3	6.22247832307142	9.527092285143759
4	3.2986984578420038	6.734080966249639
5	1.9130522844519633	4.881716660681125
6	1.2108117606694007	3.707691625397528
7	0.8267676331923579	2.9536377584539206
8	0.5872479728873506	2.3976580140498567
9	0.41783193261218543	1.919198352326941
>10	1.656552395149665	13.816035496167553
>50	0.053992774462733824	1.8355774179348634
>100	0.01605084781319135	1.337853450434778
>500	5.832175289274979E-4	0.20959540455459338
>1k	5.832175289274778E-4	0.5864206573348608
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2882	0.14260648231075942	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2319	0.11474824166504201	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2146	0.10618789418420879	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.948177734585684E-5
2	0.0	0.0	0.0	0.0	4.948177734585684E-5
3	0.0	0.0	0.0	0.0	4.948177734585684E-5
4	0.0	0.0	0.0	0.0	4.948177734585684E-5
5	0.0	0.0	0.0	0.0	4.948177734585684E-5
6	0.0	0.0	0.0	0.0	9.896355469171368E-5
7	0.0	0.0	0.0	0.0	9.896355469171368E-5
8	0.0	0.0	0.0	0.0	9.896355469171368E-5
9	0.0	0.0	0.0	0.0	9.896355469171368E-5
10-11	0.0	0.0	0.0	0.0	9.896355469171368E-5
12-13	0.0	0.0	0.0	4.948177734585684E-5	9.4015376957128E-4
14-15	0.0	0.0	0.0	9.896355469171368E-5	9.896355469171368E-4
16-17	0.0	0.0	0.0	9.896355469171368E-5	9.896355469171368E-4
18-19	0.0	0.0	0.0	1.237044433646421E-4	0.0010391173242629936
20-21	0.0	0.0	0.0	2.474088867292842E-4	0.001459712431702777
22-23	0.0	0.0	0.0	5.442995508044253E-4	0.001583416875067419
24-25	0.0	0.0	0.0	0.0014349715430298485	0.001583416875067419
26-27	0.0	0.0	0.0	0.002696756865349198	0.0016823804297591326
28-29	0.0	0.0	0.0	0.006704780830363602	0.0017813439844508463
30-31	0.0	0.0	0.0	0.018679370948060957	0.0017813439844508463
32-33	0.0	0.0	0.0	0.05091674888888669	0.00188030753914256
34-35	0.0	0.0	0.0	0.10641056218226513	0.0019792710938342736
36-37	0.0	0.0	0.0	0.1984714089342318	0.0019792710938342736
38-39	0.0	0.0	0.0	0.34441791121583654	0.0020782346485259872
40-41	0.0	0.0	0.0	0.5318796246906152	0.0020782346485259872
42-43	0.0	0.0	0.0	0.6976435787992357	0.0021029755371989155
44-45	0.0	4.948177734585684E-5	0.0	0.8874556766979425	0.0021277164258718443
46-47	0.0	4.948177734585684E-5	0.0	1.122988936864221	0.0021277164258718443
48-49	0.0	4.948177734585684E-5	0.0	1.3744800702245383	0.0022761617579094146
50-51	0.0	4.948177734585684E-5	0.0	1.6447990198649545	0.0023256435352552716
52-53	0.0	4.948177734585684E-5	0.0	1.906854512688612	0.002523570644638699
54-55	0.0	4.948177734585684E-5	0.0	2.2022854643320504	0.0026225341993304126
56-57	0.0	4.948177734585684E-5	0.0	2.5586779656655843	0.002696756865349198
58-59	0.0	4.948177734585684E-5	0.0	2.960494738602615	0.0027709795313679833
60-61	0.0	4.948177734585684E-5	0.0	3.3903181975174004	0.00282046130871384
62-63	0.0	4.948177734585684E-5	0.0	3.8311513518916387	0.0030678701954431242
64-65	0.0	4.948177734585684E-5	0.0	4.301772536228084	0.003117351972788981
66-67	0.0	4.948177734585684E-5	0.0	4.854483989181304	0.0031420928614619096
68-69	0.0	4.948177734585684E-5	0.0	5.479240909950093	0.003166833750134838
70-71	0.0	4.948177734585684E-5	0.0	6.168274659491149	0.003166833750134838
72-73	0.0	4.948177734585684E-5	0.0	6.873192059560226	0.003364760859518265
74-75	0.0	4.948177734585684E-5	0.0	7.567198727724541	0.003587428857574621
76-77	0.0	4.948177734585684E-5	0.0	8.353414687972862	0.0036121697462475496
78-79	0.0	4.948177734585684E-5	0.0	9.239435393127774	0.0037358741896121915
80-81	0.0	4.948177734585684E-5	0.0	10.17897064048223	0.00376061507828512
82-83	0.0	4.948177734585684E-5	0.0	11.15900672259427	0.0038595786329768335
84-85	0.0	4.948177734585684E-5	0.0	12.11504414269357	0.0038595786329768335
86-87	0.0	4.948177734585684E-5	0.0	13.11873251437693	0.004032764853687332
88-89	0.0	4.948177734585684E-5	0.0	14.195480730311449	0.004205951074397831
90-91	0.0	4.948177734585684E-5	0.0	15.347416506922997	0.00423069196307076
92-93	0.0	4.948177734585684E-5	0.0	16.52411791309614	0.004354396406435402
94-95	0.0	4.948177734585684E-5	0.0	17.671600329746564	0.004403878183781259
96-97	0.0	4.948177734585684E-5	0.0	18.842685554190957	0.004651287070510543
98-99	0.0	4.948177734585684E-5	0.0	20.097766095679944	0.004799732402548114
100-101	0.0	4.948177734585684E-5	0.0	21.414253522855137	0.004799732402548114
102-103	0.0	4.948177734585684E-5	0.0	22.74078575083154	0.004873955068566899
104-105	0.0	4.948177734585684E-5	0.0	24.026767662273016	0.004948177734585684
106-107	0.0	4.948177734585684E-5	0.0	25.30176461914371	0.004948177734585684
108-109	0.0	4.948177734585684E-5	0.0	26.58099226797747	0.004948177734585684
110-111	4.948177734585684E-5	9.896355469171368E-5	0.0	27.923482369147912	0.004948177734585684
112-113	4.948177734585684E-5	9.896355469171368E-5	0.0	29.288932015006836	0.004948177734585684
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	5305	0.0	29.73295	4
GTACATG	9330	0.0	29.53757	1
TACATGG	9125	0.0	28.701017	2
GTATCAA	6995	0.0	28.055798	1
CGCGTAA	55	7.755836E-4	27.044735	10-11
AACGCAG	7215	0.0	26.06536	6
ACATGGG	10045	0.0	25.420544	3
TCAACGC	7500	0.0	25.237326	4
TATCAAC	7720	0.0	25.057835	2
ATCAACG	7825	0.0	24.265198	3
CAACGCA	7890	0.0	24.214975	5
ACGCAGA	8130	0.0	23.202131	7
TCGCGTA	130	0.0020515355	22.886837	9
ATGGGGG	2670	0.0	22.514698	5
CGCAGAG	8500	0.0	21.91213	8
GGTATCA	5200	0.0	21.565968	1
GTATCGC	140	0.0031524347	21.254698	6
GAGTACT	2515	0.0	20.10881	12-13
GTCTAGA	835	0.0	20.002577	1
GCAGAGT	9115	0.0	19.650291	9
>>END_MODULE