FastQCFastQC Report
Mon 15 Aug 2016
SRR2921091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020946
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72990.3611674928474091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57740.2857077823949774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.20356803200085502No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT32290.15977665904977173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22730.11247207990713261No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20910.10346639643018665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93300.041.4882551
GGTATCA61050.040.1530231
TATCAAC115450.033.2404062
ATCAACG115400.033.151533
TCAACGC116000.032.9286884
CAACGCA118000.032.3705755
AACGCAG119750.031.9108946
ACGCAGA132450.028.8810377
CGCAGAG137000.027.8356888
GTACATG94300.025.9951421
TATACTG8100.025.7488255
GCAGAGT144550.025.4757129
AGTACTT80950.025.09857712-13
TACATGG94600.025.0706922
GAGTACT81500.025.002212-13
CATGGGG51350.024.4859184
GTACTTT86400.023.27438514-15
ACATGGG98750.023.2326093
CAGAGTA136100.021.98769610-11
AGAGTAC132950.021.92691810-11
Produced by FastQC (version 0.11.5)