FastQCFastQC Report
Mon 15 Aug 2016
SRR2921090_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921090_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000800
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113800.040.568511
GGTATCA76900.036.300271
TCAACGC130000.034.3835754
CAACGCA132950.033.441575
AACGCAG135550.032.7084856
TATCAAC140550.032.2255972
ATCAACG139800.031.973283
GTACATG212200.031.8193531
TACATGG220500.029.7454242
ACATGGG222300.028.5144213
ACGCAGA156050.028.0665327
CATGGGG162750.027.9400254
CGCAGAG167350.025.8867038
GCAGAGT187000.022.466259
AGAGTAC161650.019.74686210-11
ATGGGGG91450.019.5901265
GTATATA21550.019.3749731
CAGAGTA176150.019.03335610-11
GTATAGG10350.019.017961
GAGTACA160150.017.1697641
Produced by FastQC (version 0.11.5)