##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921090_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6000800
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.177092221037196	31.0	31.0	34.0	26.0	34.0
2	30.26585338621517	31.0	31.0	34.0	26.0	34.0
3	30.35908295560592	31.0	31.0	34.0	26.0	34.0
4	33.7545018997467	37.0	35.0	37.0	28.0	37.0
5	33.80345103986135	37.0	35.0	37.0	28.0	37.0
6	33.81183992134382	37.0	35.0	37.0	29.0	37.0
7	33.678089588054924	37.0	35.0	37.0	28.0	37.0
8	33.680824056792424	37.0	35.0	37.0	28.0	37.0
9	35.164845353952806	39.0	35.0	39.0	28.0	39.0
10-11	35.114494984002135	39.0	35.0	39.0	27.5	39.0
12-13	35.055074490067994	39.0	35.0	39.0	27.0	39.0
14-15	36.30808342221037	40.0	37.0	41.0	27.0	41.0
16-17	36.297565991201175	40.0	36.5	41.0	27.0	41.0
18-19	36.31866967737635	40.0	37.0	41.0	27.0	41.0
20-21	36.34015522930276	40.0	37.0	41.0	27.0	41.0
22-23	36.28068590854553	40.0	37.0	41.0	27.0	41.0
24-25	36.26218512531663	40.0	36.0	41.0	27.0	41.0
26-27	36.20307633982136	40.0	36.0	41.0	27.0	41.0
28-29	36.078645680575924	40.0	36.0	41.0	27.0	41.0
30-31	35.984682209038795	40.0	36.0	41.0	27.0	41.0
32-33	35.863449456739104	40.0	36.0	41.0	27.0	41.0
34-35	35.752932608985475	40.0	35.5	41.0	26.5	41.0
36-37	35.64903004599387	40.0	35.0	41.0	26.0	41.0
38-39	35.525188224903346	40.0	35.0	41.0	25.5	41.0
40-41	35.38447731969071	40.0	35.0	41.0	25.0	41.0
42-43	35.2183383882149	40.0	35.0	41.0	24.5	41.0
44-45	35.04408653846154	39.0	35.0	41.0	24.0	41.0
46-47	34.864109785361954	39.0	35.0	41.0	23.5	41.0
48-49	34.63785028662845	39.0	34.0	41.0	23.0	41.0
50-51	34.45904904346087	39.0	34.0	41.0	22.5	41.0
52-53	34.26512931609119	38.5	34.0	41.0	22.0	41.0
54-55	34.02245225636582	38.0	33.5	40.0	20.5	41.0
56-57	33.766407812291696	38.0	33.0	40.0	20.0	41.0
58-59	33.472678309558724	37.5	33.0	40.0	18.5	41.0
60-61	33.15766114518064	37.0	33.0	40.0	17.5	41.0
62-63	32.85668227569657	36.5	32.0	40.0	15.5	41.0
64-65	32.512566241167846	36.0	32.0	39.0	13.0	41.0
66-67	32.15307467337688	35.5	32.0	39.0	11.0	41.0
68-69	31.777060225303295	35.0	31.0	38.5	8.0	40.0
70-71	31.407127549660046	35.0	31.0	37.5	7.5	40.0
72-73	31.047537078389546	35.0	31.0	37.0	7.0	39.0
74-75	30.988513198240234	35.0	31.0	37.0	6.5	39.0
76-77	31.030371783762163	35.0	31.5	36.0	3.5	39.0
78-79	30.85317157712305	35.0	32.0	36.0	2.0	38.0
80-81	30.646097686975068	35.0	32.0	35.5	2.0	37.0
82-83	30.409572473670174	35.0	31.5	35.0	2.0	37.0
84-85	30.169522563658177	35.0	31.0	35.0	2.0	36.0
86-87	30.025957372350355	35.0	31.0	35.0	2.0	36.0
88-89	29.851906412478336	35.0	31.0	35.0	2.0	36.0
90-91	29.70927892947607	35.0	31.0	35.0	2.0	35.5
92-93	29.595992117717635	35.0	31.0	35.0	2.0	35.0
94-95	29.479225686575123	35.0	31.0	35.0	2.0	35.0
96-97	29.368030429276097	35.0	31.0	35.0	2.0	35.0
98-99	29.252861951739767	35.0	30.0	35.0	2.0	35.0
100-101	29.107701889748032	35.0	30.0	35.0	2.0	35.0
102-103	27.849638381549127	33.0	28.0	34.5	2.0	35.0
104-105	28.750489684708704	34.0	29.5	35.0	2.0	35.0
106-107	28.86309283762165	35.0	30.0	35.0	2.0	35.0
108-109	28.77447890281296	35.0	30.0	35.0	2.0	35.0
110-111	28.645718320890545	35.0	29.5	35.0	2.0	35.0
112-113	28.50890906212505	34.0	29.0	35.0	2.0	35.0
114-115	28.34408003932809	34.0	29.0	35.0	2.0	35.0
116-117	28.174867017730968	34.0	29.0	35.0	2.0	35.0
118-119	27.930392864284762	34.0	29.0	35.0	2.0	35.0
120-121	27.680454106119186	34.0	29.0	35.0	2.0	35.0
122-123	27.331779429409412	34.0	28.0	35.0	2.0	35.0
124-125	26.035818474203438	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	197036.0
3	29834.0
4	19976.0
5	19390.0
6	25036.0
7	26953.0
8	25571.0
9	27569.0
10	29963.0
11	32536.0
12	33271.0
13	32975.0
14	32833.0
15	28252.0
16	22834.0
17	20803.0
18	19999.0
19	20476.0
20	21717.0
21	24254.0
22	28896.0
23	37511.0
24	50324.0
25	62559.0
26	70374.0
27	79696.0
28	93039.0
29	109386.0
30	128848.0
31	158389.0
32	198307.0
33	257919.0
34	356196.0
35	545569.0
36	1031313.0
37	1607651.0
38	493084.0
39	461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.6767979368353	17.267941182024785	11.50720351240688	25.548057368733033
2	16.846756359366026	19.884853771727705	37.33535738639007	25.933032482516193
3	19.53298788023329	23.664999441293265	28.67052813630443	28.131484542169016
4	13.467931815903675	15.742174945448486	34.9615866947512	35.828306543896645
5	14.451046193021	37.268200479282385	33.53031827226155	14.75043505543506
6	34.21478281617337	35.010169672518195	16.489452406099467	14.285595105208976
7	29.29661888168939	31.872940209218186	20.390114405664125	18.4403265034283
8	27.16363278428154	34.45105664526182	19.30757077529584	19.077739795160802
9	26.630395610233354	15.649722302678734	18.549677187708973	39.17020489937894
10-11	25.8346363285835	25.63006044446713	26.9606377416317	21.574665485317666
12-13	26.124649715811337	23.107823231766417	26.904611116791305	23.862915935630937
14-15	23.517667487918917	24.5956236207049	23.926097305881157	27.960611585495027
16-17	23.93312381214355	27.29858537749387	24.854375377776613	23.91391543258597
18-19	24.016644836893857	26.558747942642952	26.10808302069012	23.31652419977307
20-21	24.74826021412185	25.85245421480038	25.76237498911643	23.636910581961338
22-23	24.54037591761175	25.513527792492773	25.692678726299818	24.253417563595665
24-25	24.234117414634078	25.71118011153317	25.814513956073398	24.240188517759353
26-27	24.03592509522232	25.850562092759233	25.845858108484553	24.267654703533893
28-29	24.108388911081995	26.092166352117594	25.770242129114955	24.02920260768545
30-31	24.2610532720474	26.001959933528045	25.52696251249768	24.210024281926874
32-33	23.985809324621833	26.100532546485955	25.505000906162213	24.40865722273
34-35	24.317409124863094	25.97395958124969	25.660011103493247	24.04862019039397
36-37	24.392270439060418	25.784217549623666	25.621727675009577	24.201784336306336
38-39	24.09582623733112	25.916577781621054	25.718280255111686	24.269315725936142
40-41	24.233782179046848	25.87306327754641	25.801315905023927	24.091838638382818
42-43	24.182170736240842	25.868900986069082	25.620084258126635	24.32884401956344
44-45	24.0621545217593	25.93761199769788	25.724027332566518	24.276206147976296
46-47	24.199920207344174	25.86212546123615	25.715989005789346	24.221965325630325
48-49	24.01248227243662	26.042344039757438	25.520370532651942	24.424803155154002
50-51	23.994261031991986	26.144856495365627	25.589945616215726	24.27093685642666
52-53	24.192110285089278	25.89396654928462	25.532372128117554	24.381551037508554
54-55	23.927715519567116	25.926679576893058	25.610534424495153	24.535070479044677
56-57	23.905773862975874	26.07078586584592	25.559119900177222	24.464320371000987
58-59	23.924771593927826	25.762434005763545	25.648415442123966	24.66437895818466
60-61	23.82071101496966	25.83659047715941	25.841535609539683	24.50116289833125
62-63	23.74725001626222	25.821258979666382	25.86664309339187	24.56484791067953
64-65	23.741233963713544	25.827212458416106	25.762511109469276	24.669042468401077
66-67	23.66821271107325	25.915602718841274	25.72165230410758	24.694532265977895
68-69	23.83279870719068	25.807836960193608	25.78103546661492	24.57832886600079
70-71	23.66479956097402	25.70893714902125	25.84523633712172	24.78102695288301
72-73	23.693657422387652	25.788687909224073	25.80485406562993	24.71280060275835
74-75	23.650980830399153	25.901786860992456	25.806272444178312	24.640959864430073
76-77	23.7528276778664	25.713337098566484	25.84357457847125	24.69026064509586
78-79	23.677526792175865	25.728777285501454	25.92408126786989	24.669614654452786
80-81	23.64799484150616	25.76339762742308	25.928828696823985	24.659778834246776
82-83	23.69153205831107	25.65920870602843	25.990443504522048	24.658815731138457
84-85	23.573168521547103	25.72779116337913	25.915029264606304	24.784011050467466
86-87	23.631710812219012	25.677243897866695	26.01330570942324	24.677739580491053
88-89	23.65438945864587	25.547167498825445	25.899446427164463	24.898996615364222
90-91	23.571802539451365	25.65921841660152	25.91968672749959	24.849292316447524
92-93	23.65433476834273	25.639630271500085	25.986290343693558	24.719744616463625
94-95	23.61614697849625	25.521848186403872	26.0465902938389	24.815414541260974
96-97	23.733865377317244	25.37378488692995	25.972676263682988	24.919673472069817
98-99	23.615859242712816	25.547337891150544	25.889912129257652	24.946890736878988
100-101	23.639065260718123	25.650713223825733	25.929978118161927	24.780243397294218
102-103	23.46634370389343	25.686846170469916	25.985004775740308	24.86180534989635
104-105	23.43384062248815	25.8627417628048	25.813046769992226	24.89037084471482
106-107	23.515297856108518	25.918356726810206	25.690629343849587	24.875716073231697
108-109	23.459267115577703	26.032603387941418	25.713621847037142	24.79450764944373
110-111	23.57131860948922	26.26070767787142	25.602206024737217	24.56576768790214
112-113	23.642840888595074	26.338735033407247	25.626612523753465	24.391811554244214
114-115	23.600603995591293	26.61125814957047	25.41593499558329	24.372202859254948
116-117	23.5798088493686	26.617596165312523	25.39127838760331	24.411316597715565
118-119	23.819833032284016	26.695572515417552	25.18744635187765	24.297148100420777
120-121	23.792772990966178	26.858991421107913	25.207048589699006	24.141186998226903
122-123	23.911141718782734	27.0047394819394	25.066548891252104	24.017569908025763
124-125	24.08121790489422	27.198507447249	24.81192113700129	23.90835351085549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2429.0
1	1972.5
2	1368.5
3	1040.5
4	801.0
5	713.0
6	668.5
7	705.5
8	820.0
9	885.5
10	868.5
11	863.5
12	974.0
13	1125.5
14	1274.5
15	1455.5
16	1675.0
17	1987.5
18	2405.0
19	2864.0
20	3454.5
21	4277.0
22	5312.5
23	6698.5
24	8449.0
25	10847.5
26	14326.0
27	18969.0
28	24039.5
29	29773.0
30	36615.5
31	44924.0
32	56210.5
33	64547.0
34	70228.0
35	78299.0
36	88744.0
37	100207.5
38	112026.5
39	123850.5
40	135504.5
41	148081.0
42	159550.5
43	174370.5
44	191318.0
45	208161.0
46	224694.5
47	236759.0
48	248757.0
49	258087.0
50	262818.0
51	267569.0
52	267457.5
53	266399.5
54	263916.0
55	250916.0
56	233084.5
57	213227.5
58	192946.0
59	170633.0
60	145874.0
61	124057.0
62	102485.0
63	78497.0
64	57754.5
65	44479.0
66	34860.5
67	27124.5
68	20499.0
69	15126.0
70	11802.5
71	9274.0
72	6898.5
73	5345.0
74	4286.0
75	3288.0
76	2295.5
77	1416.0
78	953.5
79	710.5
80	469.0
81	331.0
82	261.5
83	177.5
84	140.0
85	118.5
86	94.0
87	73.5
88	54.5
89	44.0
90	40.0
91	41.5
92	35.0
93	21.5
94	17.5
95	14.5
96	12.0
97	13.0
98	17.0
99	19.5
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28301226503132915
2	0.07084055459272097
3	0.08015597920277297
4	0.08483868817491001
5	0.09310425276629783
6	0.12481669110785229
7	0.11441807758965472
8	0.11381815757898947
9	0.12038394880682576
10-11	0.09929509398746833
12-13	0.08850486601786428
14-15	0.09815357952273031
16-17	0.10032828956139182
18-19	0.09244600719904013
20-21	0.09202106385815224
22-23	0.07431509132115718
24-25	0.08639681375816557
26-27	0.09802026396480469
28-29	0.09073790161311825
30-31	0.08646347153712838
32-33	0.09559558725503267
34-35	0.08981302493000932
36-37	0.08914644714038128
38-39	0.07931442474336756
40-41	0.09096287161711772
42-43	0.08033095587255032
44-45	0.07637315024663378
46-47	0.06678276229836022
48-49	0.06891581122516997
50-51	0.07084888681509131
52-53	0.083222237035062
54-55	0.08822990267964272
56-57	0.09624550059992001
58-59	0.06614951339821357
60-61	0.0834055459272097
62-63	0.08848820157312358
64-65	0.07980602586321824
66-67	0.07369017464338089
68-69	0.08108085588588188
70-71	0.0799810025329956
72-73	0.06171677109718704
74-75	0.08948806825756567
76-77	0.09328756165844554
78-79	0.07509832022397013
80-81	0.08751333155579256
82-83	0.09175443274230104
84-85	0.09597886948406878
86-87	0.08378882815624583
88-89	0.06411645113984801
90-91	0.08753832822290361
92-93	0.06430809225436608
94-95	0.09122117051059858
96-97	0.08006432475669911
98-99	0.07391514464738035
100-101	0.09027129716037863
102-103	0.06372483668844155
104-105	0.07032395680575923
106-107	0.08755499266764431
108-109	0.09374583388881483
110-111	0.08697173710171978
112-113	0.07616484468737503
114-115	0.08985468604186109
116-117	0.0834055459272097
118-119	0.07583988801493134
120-121	0.1082272363684842
122-123	0.08678842820957205
124-125	0.10841887748300226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	6000800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.68611327384289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.00116550707916	29.707066190955985
2	14.46301795108317	12.63666080985285
3	6.260781388796372	8.205276148011771
4	3.3529683463536473	5.859126199296604
5	1.9333310288293433	4.2229859160636956
6	1.3003865721340526	3.40853010540195
7	0.8794083865289506	2.68925540715098
8	0.632765484522854	2.211445170611478
9	0.46780391185102227	1.8392881214763517
>10	2.5012725638876234	19.471495966727094
>50	0.14454855208842637	4.290539360140533
>100	0.059907633479593714	4.324655604716894
>500	0.0017617970270843153	0.5087095569069636
>1k	8.808763386665443E-4	0.6249654426869322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1665111318490868E-4
2	0.0	0.0	0.0	0.0	1.1665111318490868E-4
3	0.0	0.0	0.0	0.0	1.1665111318490868E-4
4	0.0	0.0	0.0	0.0	1.333155579256099E-4
5	0.0	0.0	0.0	0.0	1.333155579256099E-4
6	0.0	0.0	0.0	0.0	2.3330222636981735E-4
7	0.0	0.0	0.0	0.0	2.499666711105186E-4
8	0.0	0.0	0.0	0.0	2.499666711105186E-4
9	0.0	0.0	0.0	0.0	2.666311158512198E-4
10-11	0.0	0.0	0.0	0.0	3.2495667244367415E-4
12-13	0.0	0.0	0.0	0.0	6.332489001466471E-4
14-15	0.0	0.0	0.0	0.0	7.748966804426076E-4
16-17	0.0	0.0	0.0	0.0	9.748700173310225E-4
18-19	0.0	0.0	0.0	1.666444474070124E-5	0.0010498600186641782
20-21	0.0	0.0	0.0	4.9993334222103715E-5	0.0011915077989601386
22-23	0.0	0.0	0.0	1.5831222503666177E-4	0.0012165044660711905
24-25	0.0	0.0	0.0	3.0829222770297295E-4	0.0012415011331822425
26-27	0.0	0.0	0.0	8.082255699240102E-4	0.0013164911345153979
28-29	0.0	0.0	0.0	0.003341221170510599	0.0013331555792560992
30-31	0.0	0.0	0.0	0.014556392481002533	0.0013664844687375018
32-33	0.0	0.0	0.0	0.04309425409945341	0.0014164778029596055
34-35	0.0	1.666444474070124E-5	0.0	0.08963804826023197	0.0014831355819224104
36-37	0.0	1.666444474070124E-5	0.0	0.17307692307692307	0.0014998000266631115
38-39	0.0	1.666444474070124E-5	0.0	0.3088671510465271	0.0015747900279962672
40-41	0.0	1.666444474070124E-5	0.0	0.4654379416077856	0.001599786695107319
42-43	0.0	1.666444474070124E-5	0.0	0.6240334622050393	0.0016081189174776698
44-45	0.0	1.666444474070124E-5	0.0	0.8249233435541927	0.0016997733635515265
46-47	0.0	1.666444474070124E-5	0.0	1.0685908545527263	0.0018247566991067858
48-49	0.0	1.666444474070124E-5	0.0	1.3226569790694573	0.0018997467004399414
50-51	0.0	1.666444474070124E-5	0.0	1.5910961871750433	0.001974736701773097
52-53	0.0	1.666444474070124E-5	0.0	1.8668844154112785	0.0021413811491801096
54-55	0.0	1.666444474070124E-5	0.0	2.1837838288228237	0.0021997067057725637
56-57	0.0	1.666444474070124E-5	0.0	2.561925076656446	0.0022247033728836157
58-59	0.0	1.666444474070124E-5	0.0	2.971187175043328	0.002249700039994667
60-61	0.0	1.666444474070124E-5	0.0	3.4101369817357687	0.0022913611518464204
62-63	0.0	1.666444474070124E-5	0.0	3.847886948406879	0.002383015597920277
64-65	0.0	1.666444474070124E-5	0.0	4.320715571257166	0.0024163444874016797
66-67	0.0	1.666444474070124E-5	0.0	4.873075256632449	0.002483002266364485
68-69	0.0	1.666444474070124E-5	0.0	5.488184908678843	0.0025246633782162377
70-71	0.0	1.666444474070124E-5	0.0	6.143630849220104	0.0025746567124383416
72-73	0.0	1.666444474070124E-5	0.0	6.807350686575123	0.0027413011598453538
74-75	8.33222237035062E-6	1.666444474070124E-5	0.0	7.498708505532596	0.002866284495400613
76-77	1.666444474070124E-5	1.666444474070124E-5	0.0	8.26995567257699	0.0029246100519930676
78-79	1.666444474070124E-5	1.666444474070124E-5	0.0	9.121783762165045	0.0030162644980669243
80-81	1.666444474070124E-5	1.666444474070124E-5	0.0	10.0094987335022	0.00309125449940008
82-83	1.666444474070124E-5	1.666444474070124E-5	0.0	10.922935275296627	0.003216237834955339
84-85	1.666444474070124E-5	1.666444474070124E-5	0.0	11.828939474736702	0.0032245700573256897
86-87	1.666444474070124E-5	1.666444474070124E-5	0.0	12.79636048526863	0.003266231169177443
88-89	1.666444474070124E-5	1.666444474070124E-5	0.0	13.830397613651513	0.003316224503399547
90-91	1.666444474070124E-5	2.4996667111051858E-5	0.0	14.927168044260766	0.003332888948140248
92-93	1.666444474070124E-5	3.332888948140248E-5	0.0	16.030137648313556	0.003424543394214105
94-95	1.666444474070124E-5	3.332888948140248E-5	0.0	17.129274430075988	0.0034912011731769096
96-97	3.332888948140248E-5	3.332888948140248E-5	0.0	18.25525763231569	0.003724503399546727
98-99	3.332888948140248E-5	3.332888948140248E-5	0.0	19.433383882149048	0.003857818957472337
100-101	3.332888948140248E-5	3.332888948140248E-5	0.0	20.6725769897347	0.0038661511798426876
102-103	3.332888948140248E-5	3.332888948140248E-5	0.0	21.913803159578723	0.003924476736435142
104-105	3.332888948140248E-5	3.332888948140248E-5	0.0	23.133290561258498	0.003949473403546194
106-107	3.332888948140248E-5	3.332888948140248E-5	0.0	24.343995800559924	0.003949473403546194
108-109	3.332888948140248E-5	3.332888948140248E-5	0.0	25.577631315824554	0.003957805625916544
110-111	3.332888948140248E-5	3.332888948140248E-5	0.0	26.86238501533129	0.00404112784962005
112-113	3.332888948140248E-5	3.332888948140248E-5	0.0	28.15475436608452	0.004066124516731102
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11380	0.0	40.56851	1
GGTATCA	7690	0.0	36.30027	1
TCAACGC	13000	0.0	34.383575	4
CAACGCA	13295	0.0	33.44157	5
AACGCAG	13555	0.0	32.708485	6
TATCAAC	14055	0.0	32.225597	2
ATCAACG	13980	0.0	31.97328	3
GTACATG	21220	0.0	31.819353	1
TACATGG	22050	0.0	29.745424	2
ACATGGG	22230	0.0	28.514421	3
ACGCAGA	15605	0.0	28.066532	7
CATGGGG	16275	0.0	27.940025	4
CGCAGAG	16735	0.0	25.886703	8
GCAGAGT	18700	0.0	22.46625	9
AGAGTAC	16165	0.0	19.746862	10-11
ATGGGGG	9145	0.0	19.590126	5
GTATATA	2155	0.0	19.374973	1
CAGAGTA	17615	0.0	19.033356	10-11
GTATAGG	1035	0.0	19.01796	1
GAGTACA	16015	0.0	17.169764	1
>>END_MODULE